LOCUS       NM_007066               1101 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens cAMP-dependent protein kinase inhibitor gamma (PKIG),
            transcript variant 2, mRNA.
ACCESSION   NM_007066
VERSION     NM_007066.5
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Chen,X., Hausman,B.S., Luo,G., Zhou,G., Murakami,S., Rubin,J. and
            Greenfield,E.M.
  TITLE     Protein kinase inhibitor gamma reciprocally regulates osteoblast
            and adipocyte differentiation by downregulating leukemia inhibitory
            factor
  JOURNAL   Stem Cells 31 (12), 2789-2799 (2013)
   PUBMED   23963683
  REMARK    GeneRIF: Results show that endogenous levels of Pkig reciprocally
            regulate osteoblast and adipocyte differentiation and that this
            reciprocal regulation is mediated in part by LIF.
REFERENCE   3  (bases 1 to 1101)
  AUTHORS   Zhao,L., Yang,S., Zhou,G.Q., Yang,J., Ji,D., Sabatakos,G. and
            Zhu,T.
  TITLE     Downregulation of cAMP-dependent protein kinase inhibitor gamma is
            required for BMP-2-induced osteoblastic differentiation
  JOURNAL   Int J Biochem Cell Biol 38 (12), 2064-2073 (2006)
   PUBMED   16870489
  REMARK    GeneRIF: These results indicate that the downregulation of PKIgamma
            may be prerequisite for the PKA activation during the osteoblastic
            differentiation of precursor cells.
REFERENCE   4  (bases 1 to 1101)
  AUTHORS   Yu,J., Yu,L., Chen,Z., Zheng,L., Chen,X., Wang,X., Ren,D. and
            Zhao,S.
  TITLE     Protein inhibitor of neuronal nitric oxide synthase interacts with
            protein kinase A inhibitors
  JOURNAL   Brain Res Mol Brain Res 99 (2), 145-149 (2002)
   PUBMED   11978406
REFERENCE   5  (bases 1 to 1101)
  AUTHORS   Lum,H., Hao,Z., Gayle,D., Kumar,P., Patterson,C.E. and Uhler,M.D.
  TITLE     Vascular endothelial cells express isoforms of protein kinase A
            inhibitor
  JOURNAL   Am J Physiol Cell Physiol 282 (1), C59-C66 (2002)
   PUBMED   11742798
  REMARK    GeneRIF: Functional studies of the mouse homolog
REFERENCE   6  (bases 1 to 1101)
  AUTHORS   Zheng,L., Yu,L., Tu,Q., Zhang,M., He,H., Chen,W., Gao,J., Yu,J.,
            Wu,Q. and Zhao,S.
  TITLE     Cloning and mapping of human PKIB and PKIG, and comparison of
            tissue expression patterns of three members of the protein kinase
            inhibitor family, including PKIA
  JOURNAL   Biochem J 349 (Pt 2), 403-407 (2000)
   PUBMED   10880337
REFERENCE   7  (bases 1 to 1101)
  AUTHORS   Collins,S.P. and Uhler,M.D.
  TITLE     Characterization of PKIgamma, a novel isoform of the protein kinase
            inhibitor of cAMP-dependent protein kinase
  JOURNAL   J Biol Chem 272 (29), 18169-18178 (1997)
   PUBMED   9218452
  REMARK    GeneRIF: Functional studies of the mouse homolog
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC104257.1 and Z97053.2.
            
            On May 10, 2019 this sequence version replaced NM_007066.4.
            
            Summary: This gene encodes a member of the protein kinase inhibitor
            family. Studies of a similar protein in mice suggest that this
            protein acts as a potent competitive cAMP-dependent protein kinase
            inhibitor, and is a predominant form of inhibitor in various
            tissues. The encoded protein may be involved in osteogenesis.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 4. Variants 1-5 encode the same protein.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1965299,
                              SAMEA1966682 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-626               BC104257.1         69-694
            627-1101            Z97053.2           135469-135943
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..1101
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="cAMP-dependent protein kinase inhibitor gamma"
                     /db_xref="GeneID:11142"
                     /db_xref="HGNC:HGNC:9019"
                     /db_xref="MIM:604932"
     exon            1..104
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            105..174
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            175..348
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     CDS             198..428
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="protein kinase (cAMP-dependent, catalytic)
                     inhibitor gamma"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase inhibitor gamma"
                     /protein_id="NP_008997.1"
                     /db_xref="CCDS:CCDS13334.1"
                     /db_xref="GeneID:11142"
                     /db_xref="HGNC:HGNC:9019"
                     /db_xref="MIM:604932"
                     /translation="MMEVESSYSDFISCDRTGRRNAVPDIQGDSEAVSVRKLAGDMGE
                     LALEGAEGQVEGSAPDKEAGNQPQSSDGTTSS"
     exon            349..1101
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1078..1083
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="hexamer: AATAAA"
     polyA_site      1101
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="major polyA site"
ORIGIN      
        1 aaggcaacgg gctccgctgc gggttgcggg ctgcgggcgc taggctgccc cggggaggcg
       61 ctgcggaccg gcggcccagg cacggagagg cggcgagacc gcagaattcc cgtactgatt
      121 agtcaacagt ggaaaatctg aagagatgca agcaggaaaa agaaattaaa ccaggcctga
      181 ggagcgatgc gacaggcatg atggaggtcg agtcctccta ctcggacttc atctcctgtg
      241 accggacagg ccgtcggaat gcggtccctg acatccaggg agactcagag gctgtgagcg
      301 tgaggaagct ggctggagac atgggcgagc tggcactcga gggggcagaa ggacaggtgg
      361 agggaagcgc cccagacaag gaagctggca accagcccca gagcagcgat gggaccacct
      421 cgtcttgaat ctgaccttgt ccaagaaggc tggacgagag accttctgtc ccctcccaga
      481 gggggaaccc tggcactggc ccagcagcct cttctctgag ctccatgtcc cagataaacc
      541 aggccagact gagaaggctc cccagaggcc tctgtggcct ccactccggg aaagccctct
      601 gcccacaccc acaggcttca cattcccacc accttcgcac cgtgcccagg tacactttca
      661 agacactgta accacaagat gttatttatt gagctggcgc cgggacttgg gcggggcctg
      721 ccctacagtg agcagcccac acaggaacgc tcctctcgcg agcggcccgg gcagggaccc
      781 tgtcccaaca ccaacacctc ctctccagcc caatcttctg ggtccagacc tgcttgtccc
      841 ttttttagaa aacactttta aactttttaa aaattttaaa ccttttttca gcagatatgg
      901 agagagctga caatcaattc acatttttta agccatttta gctaaactgt cattgtgcat
      961 ctctgaggtt ccctcatgga gctccacaga tccattttta gggaagggat tttggctcaa
     1021 aacgatctga ccacctctgc cctgtccacc aggataagtg acacctagga cccaggaaat
     1081 aaatgccgat gatttgtgtg a
//
