LOCUS       NM_001281445            1150 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens cAMP-dependent protein kinase inhibitor gamma (PKIG),
            transcript variant 5, mRNA.
ACCESSION   NM_001281445
VERSION     NM_001281445.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Chen,X., Hausman,B.S., Luo,G., Zhou,G., Murakami,S., Rubin,J. and
            Greenfield,E.M.
  TITLE     Protein kinase inhibitor gamma reciprocally regulates osteoblast
            and adipocyte differentiation by downregulating leukemia inhibitory
            factor
  JOURNAL   Stem Cells 31 (12), 2789-2799 (2013)
   PUBMED   23963683
  REMARK    GeneRIF: Results show that endogenous levels of Pkig reciprocally
            regulate osteoblast and adipocyte differentiation and that this
            reciprocal regulation is mediated in part by LIF.
REFERENCE   3  (bases 1 to 1150)
  AUTHORS   Zhao,L., Yang,S., Zhou,G.Q., Yang,J., Ji,D., Sabatakos,G. and
            Zhu,T.
  TITLE     Downregulation of cAMP-dependent protein kinase inhibitor gamma is
            required for BMP-2-induced osteoblastic differentiation
  JOURNAL   Int J Biochem Cell Biol 38 (12), 2064-2073 (2006)
   PUBMED   16870489
  REMARK    GeneRIF: These results indicate that the downregulation of PKIgamma
            may be prerequisite for the PKA activation during the osteoblastic
            differentiation of precursor cells.
REFERENCE   4  (bases 1 to 1150)
  AUTHORS   Yu,J., Yu,L., Chen,Z., Zheng,L., Chen,X., Wang,X., Ren,D. and
            Zhao,S.
  TITLE     Protein inhibitor of neuronal nitric oxide synthase interacts with
            protein kinase A inhibitors
  JOURNAL   Brain Res Mol Brain Res 99 (2), 145-149 (2002)
   PUBMED   11978406
REFERENCE   5  (bases 1 to 1150)
  AUTHORS   Lum,H., Hao,Z., Gayle,D., Kumar,P., Patterson,C.E. and Uhler,M.D.
  TITLE     Vascular endothelial cells express isoforms of protein kinase A
            inhibitor
  JOURNAL   Am J Physiol Cell Physiol 282 (1), C59-C66 (2002)
   PUBMED   11742798
  REMARK    GeneRIF: Functional studies of the mouse homolog
REFERENCE   6  (bases 1 to 1150)
  AUTHORS   Zheng,L., Yu,L., Tu,Q., Zhang,M., He,H., Chen,W., Gao,J., Yu,J.,
            Wu,Q. and Zhao,S.
  TITLE     Cloning and mapping of human PKIB and PKIG, and comparison of
            tissue expression patterns of three members of the protein kinase
            inhibitor family, including PKIA
  JOURNAL   Biochem J 349 (Pt 2), 403-407 (2000)
   PUBMED   10880337
REFERENCE   7  (bases 1 to 1150)
  AUTHORS   Collins,S.P. and Uhler,M.D.
  TITLE     Characterization of PKIgamma, a novel isoform of the protein kinase
            inhibitor of cAMP-dependent protein kinase
  JOURNAL   J Biol Chem 272 (29), 18169-18178 (1997)
   PUBMED   9218452
  REMARK    GeneRIF: Functional studies of the mouse homolog
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AA463287.1, DN994035.1 and
            BQ010165.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001281445.1.
            
            Summary: This gene encodes a member of the protein kinase inhibitor
            family. Studies of a similar protein in mice suggest that this
            protein acts as a potent competitive cAMP-dependent protein kinase
            inhibitor, and is a predominant form of inhibitor in various
            tissues. The encoded protein may be involved in osteogenesis.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (5) differs in the 5' UTR,
            compared to variant 4. Variants 1-5 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.351591.1,
                                           SRR5189655.54449.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372886.6/ ENSP00000361977.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AA463287.1         31-34
            5-601               DN994035.1         17-613
            602-1150            BQ010165.1         18-566              c
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..1150
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="cAMP-dependent protein kinase inhibitor gamma"
                     /db_xref="GeneID:11142"
                     /db_xref="HGNC:HGNC:9019"
                     /db_xref="MIM:604932"
     exon            1..153
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            154..223
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     exon            224..397
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     CDS             247..477
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="protein kinase (cAMP-dependent, catalytic)
                     inhibitor gamma"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase inhibitor gamma"
                     /protein_id="NP_001268374.1"
                     /db_xref="CCDS:CCDS13334.1"
                     /db_xref="GeneID:11142"
                     /db_xref="HGNC:HGNC:9019"
                     /db_xref="MIM:604932"
                     /translation="MMEVESSYSDFISCDRTGRRNAVPDIQGDSEAVSVRKLAGDMGE
                     LALEGAEGQVEGSAPDKEAGNQPQSSDGTTSS"
     exon            398..1150
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1127..1132
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="hexamer: AATAAA"
     polyA_site      1150
                     /gene="PKIG"
                     /gene_synonym="PKI-gamma"
                     /note="major polyA site"
ORIGIN      
        1 atttaggaga cacggggaag agacagagag gagggtaaag tgagaatcct gcccgcacca
       61 caggtctgga ctgctaacct tgaatcctgg tttcttaaat tctgcctccg cttctgactg
      121 aggaccactg gattttgagg aaactttggt cttaattccc gtactgatta gtcaacagtg
      181 gaaaatctga agagatgcaa gcaggaaaaa gaaattaaac caggcctgag gagcgatgcg
      241 acaggcatga tggaggtcga gtcctcctac tcggacttca tctcctgtga ccggacaggc
      301 cgtcggaatg cggtccctga catccaggga gactcagagg ctgtgagcgt gaggaagctg
      361 gctggagaca tgggcgagct ggcactcgag ggggcagaag gacaggtgga gggaagcgcc
      421 ccagacaagg aagctggcaa ccagccccag agcagcgatg ggaccacctc gtcttgaatc
      481 tgaccttgtc caagaaggct ggacgagaga ccttctgtcc cctcccagag ggggaaccct
      541 ggcactggcc cagcagcctc ttctctgagc tccatgtccc agataaacca ggccagactg
      601 agaaggctcc ccagaggcct ctgtggcctc cactccggga aagccctctg cccacaccca
      661 caggcttcac attcccacca ccttcgcacc gtgcccaggt acactttcaa gacactgtaa
      721 ccacaagatg ttatttattg agctggcgcc gggacttggg cggggcctgc cctacagtga
      781 gcagcccaca caggaacgct cctctcgcga gcggcccggg cagggaccct gtcccaacac
      841 caacacctcc tctccagccc aatcttctgg gtccagacct gcttgtccct tttttagaaa
      901 acacttttaa actttttaaa aattttaaac cttttttcag cagatatgga gagagctgac
      961 aatcaattca cattttttaa gccattttag ctaaactgtc attgtgcatc tctgaggttc
     1021 cctcatggag ctccacagat ccatttttag ggaagggatt ttggctcaaa acgatctgac
     1081 cacctctgcc ctgtccacca ggataagtga cacctaggac ccaggaaata aatgccgatg
     1141 atttgtgtga
//
