LOCUS       NM_181795               1706 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens cAMP-dependent protein kinase inhibitor beta (PKIB),
            transcript variant 1, mRNA.
ACCESSION   NM_181795
VERSION     NM_181795.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Liu,C., Wang,H., Yang,M., Liang,Y., Jiang,L., Sun,S. and Fan,S.
  TITLE     Downregulation of cAMP-Dependent Protein Kinase Inhibitor-b
            Promotes Preeclampsia by Decreasing Phosphorylated Akt
  JOURNAL   Reprod Sci 28 (1), 178-185 (2021)
   PUBMED   32676926
  REMARK    GeneRIF: Downregulation of cAMP-Dependent Protein Kinase
            Inhibitor-b Promotes Preeclampsia by Decreasing Phosphorylated Akt.
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1706)
  AUTHORS   Dou,P., Zhang,D., Cheng,Z., Zhou,G. and Zhang,L.
  TITLE     PKIB promotes cell proliferation and the invasion-metastasis
            cascade through the PI3K/Akt pathway in NSCLC cells
  JOURNAL   Exp Biol Med (Maywood) 241 (17), 1911-1918 (2016)
   PUBMED   27325557
  REMARK    GeneRIF: results indicate that PKIB promotes cell proliferation and
            tumorigenesis by activating the PI3K/Akt pathway in non-small cell
            lung cancer (NSCLC), implying that this is an important underlying
            mechanism that affects the progression of NSCLC
REFERENCE   4  (bases 1 to 1706)
  AUTHORS   Kovacs,Z., Schacht,T., Herrmann,A.K., Albrecht,P., Lefkimmiatis,K.
            and Methner,A.
  TITLE     Protein kinase inhibitor beta enhances the constitutive activity of
            G-protein-coupled zinc receptor GPR39
  JOURNAL   Biochem J 462 (1), 125-132 (2014)
   PUBMED   24869658
  REMARK    GeneRIF: mutation of the pseudosubstrate domain abolished the
            inhibitory activity of PKIB on protein kinase A activity, but had
            no effect on the interaction with GPR39, cell protection and
            induction of SRE-dependent transcription
REFERENCE   5  (bases 1 to 1706)
  AUTHORS   Chung,S., Furihata,M., Tamura,K., Uemura,M., Daigo,Y., Nasu,Y.,
            Miki,T., Shuin,T., Fujioka,T., Nakamura,Y. and Nakagawa,H.
  TITLE     Overexpressing PKIB in prostate cancer promotes its aggressiveness
            by linking between PKA and Akt pathways
  JOURNAL   Oncogene 28 (32), 2849-2859 (2009)
   PUBMED   19483721
  REMARK    GeneRIF: Findings show that PKIB and PKA-C kinase can have critical
            functions of aggressive phenotype of PCs through Akt
            phosphorylation and that they should be a promising molecular
            target for PC treatment.
REFERENCE   6  (bases 1 to 1706)
  AUTHORS   Qi,Z.Y., Hui,G.Z., Li,Y., Gu,S.H. and Xie,Y.
  TITLE     [Expression and characterizations of a novel full-length gene
            PKIbeta related to human glioma]
  JOURNAL   Zhonghua Yi Xue Za Zhi 88 (25), 1778-1782 (2008)
   PUBMED   19035091
  REMARK    GeneRIF: PKIbeta may be a novel full-length gene related to human
            glioma and may provide a new way to gene therapy of glioma.
REFERENCE   7  (bases 1 to 1706)
  AUTHORS   Yu,J., Yu,L., Chen,Z., Zheng,L., Chen,X., Wang,X., Ren,D. and
            Zhao,S.
  TITLE     Protein inhibitor of neuronal nitric oxide synthase interacts with
            protein kinase A inhibitors
  JOURNAL   Brain Res Mol Brain Res 99 (2), 145-149 (2002)
   PUBMED   11978406
REFERENCE   8  (bases 1 to 1706)
  AUTHORS   Zheng,L., Yu,L., Tu,Q., Zhang,M., He,H., Chen,W., Gao,J., Yu,J.,
            Wu,Q. and Zhao,S.
  TITLE     Cloning and mapping of human PKIB and PKIG, and comparison of
            tissue expression patterns of three members of the protein kinase
            inhibitor family, including PKIA
  JOURNAL   Biochem J 349 (Pt 2), 403-407 (2000)
   PUBMED   10880337
REFERENCE   9  (bases 1 to 1706)
  AUTHORS   Scarpetta,M.A. and Uhler,M.D.
  TITLE     Evidence for two additional isoforms of the endogenous protein
            kinase inhibitor of cAMP-dependent protein kinase in mouse
  JOURNAL   J Biol Chem 268 (15), 10927-10931 (1993)
   PUBMED   7684369
  REMARK    GeneRIF: Functional studies of the mouse homolog
REFERENCE   10 (bases 1 to 1706)
  AUTHORS   Van Patten,S.M., Ng,D.C., Th'ng,J.P., Angelos,K.L., Smith,A.J. and
            Walsh,D.A.
  TITLE     Molecular cloning of a rat testis form of the inhibitor protein of
            cAMP-dependent protein kinase
  JOURNAL   Proc Natl Acad Sci U S A 88 (12), 5383-5387 (1991)
   PUBMED   2052616
  REMARK    GeneRIF: Functional studies of the rat homolog
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP260889.1, AK315151.1,
            AL512283.12 and BU753807.1.
            
            On Nov 23, 2018 this sequence version replaced NM_181795.2.
            
            Summary: This gene encodes a member of the cAMP-dependent protein
            kinase inhibitor family. The encoded protein may play a role in the
            protein kinase A (PKA) pathway by interacting with the catalytic
            subunit of PKA, and overexpression of this gene may play a role in
            prostate cancer. Alternatively spliced transcript variants encoding
            multiple isoforms have been observed for this gene. [provided by
            RefSeq, Jul 2012].
            
            Transcript Variant: This variant (1) encodes the shorter isoform
            (1). Variants 1, 2, 3 and 4 encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.356684.1,
                                           SRR3476690.779839.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368452.7/ ENSP00000357437.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-71                BP260889.1         174-244
            72-528              AK315151.1         1-457
            529-1404            AL512283.12        135881-136756
            1405-1706           BU753807.1         17-318              c
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q22.31"
     gene            1..1706
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /note="cAMP-dependent protein kinase inhibitor beta"
                     /db_xref="GeneID:5570"
                     /db_xref="HGNC:HGNC:9018"
                     /db_xref="MIM:606914"
     exon            1..131
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /inference="alignment:Splign:2.1.0"
     exon            132..216
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /inference="alignment:Splign:2.1.0"
     exon            217..283
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /inference="alignment:Splign:2.1.0"
     exon            284..460
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             292..528
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cAMP-dependent protein kinase inhibitor 2; PKI-beta;
                     protein kinase (cAMP-dependent, catalytic) inhibitor beta"
                     /codon_start=1
                     /product="cAMP-dependent protein kinase inhibitor beta
                     isoform 1"
                     /protein_id="NP_861460.1"
                     /db_xref="CCDS:CCDS5126.1"
                     /db_xref="GeneID:5570"
                     /db_xref="HGNC:HGNC:9018"
                     /db_xref="MIM:606914"
                     /translation="MRTDSSKMTDVESGVANFASSARAGRRNALPDIQSSAATDGTSD
                     LPLKLEALSVKEDAKEKDEKTTQDQLEKPQNEEK"
     exon            461..1706
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1202..1207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /note="hexamer: AATGAA"
     polyA_site      1225
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /note="major polyA site"
     regulatory      1686..1691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /note="hexamer: ATTAAA"
     polyA_site      1706
                     /gene="PKIB"
                     /gene_synonym="PRKACN2"
                     /note="major polyA site"
ORIGIN      
        1 agacgcggcc gcatcccggt ggactgtaga ggcggcagcg agctagagcc cgagtcgcag
       61 ctccgggccg cagagcgctg ggcgagcgcg agcgccaggg caccggcagg gcaggcagct
      121 gcgcgcggct ggagtcatgc tatactgaaa agacacttca tcaagataac tctgggagaa
      181 gcagaaaacc ctgtgccagg gacaggaaag ataggagaaa gaaagtttat cagaattttt
      241 taaacctgtc tcagaaataa caacatattt taatcagaga tttatgttgc tatgaggaca
      301 gattcatcaa aaatgactga cgtggagtct ggggtcgcca attttgcatc ttcagcaagg
      361 gcaggccgcc ggaatgcctt accagacatc cagagttcag ctgccacaga cggaacctca
      421 gatttgcccc tcaaactgga ggctctctcc gtgaaggaag atgcaaaaga gaaagatgaa
      481 aaaacaacac aagaccaatt ggaaaagcct caaaatgaag aaaaatgaag gctcataatc
      541 tatcaagagt gctgaatttc tgcatgttga aagacttagt ggttctgttt tcttgagaca
      601 tttaatctgg tggtaactgt ggtaacattg cagccctaag cagcatgtgt atattagata
      661 attgtgttgt gatgctactc actttgattg caatgatgat gtccaaggta agctattaaa
      721 aggcaggtta cttccaaatc gcactgaagg aaaaggttaa gaataataca tgatcacaga
      781 aatgcatacc actgtctgta aacccaacaa aattcactgt tctcttttgg atttatttag
      841 cctgatgtat ttttaattca atttttatgg tgatgggcaa atcattcttg gtaaatgtaa
      901 atcaaacatg attgatttaa aacttcatgg aatttgtaga aaattatgga catttttggt
      961 gagaaagaac aatagtcaaa actcacatgg atagagtgtg tttgtttttt gccaaaaatg
     1021 ccccagactt tttcccaaac ctcaaaaacg tcttggaaaa attgtaaaag tttgataaca
     1081 gaaacatctt taggatattt ttgtctgaca tattttgctt ctagtatgtg cctactgtga
     1141 tttttttcat gtggaaaatg caaaatttgt aacaaaatgg ttatatggaa catgcctatt
     1201 aaatgaattt tactatcttc cctaactttg gtctgtgtat gtgtgtgtgt tttactttaa
     1261 tatgaattat acaaaatact agttgtttta cactctcttt tcttattctt agggcttttg
     1321 tgtatgtctg acttgttttt aaataacttc ctcagcaatg cagaccttaa tttttatatt
     1381 tttttaaagt agctaacata gcagtaggca cttaagcatt tagtcaatga tattggtaga
     1441 aatagtaaaa tacatccttt aaatatatat ctaagcatat attttaaaag gagcaaaaat
     1501 aaaaccaaag tgttagtaaa ttttgattta ttagatattt tagaaaaata atagaattct
     1561 gaagttttaa aaatgtcagt aattaattta ttttcatttt cagaaatata tgcatgcagt
     1621 tatgttttat ttgattgttg acttaggcta tgtctgtata cagtaaccaa ataaactctt
     1681 tcactattaa agagatttct tactga
//
