LOCUS       NM_014819               4856 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), mRNA.
ACCESSION   NM_014819
VERSION     NM_014819.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Schiefer,S. and Hale,B.G.
  TITLE     Proximal protein landscapes of the type I interferon signaling
            cascade reveal negative regulation by PJA2
  JOURNAL   Nat Commun 15 (1), 4484 (2024)
   PUBMED   38802340
  REMARK    GeneRIF: Proximal protein landscapes of the type I interferon
            signaling cascade reveal negative regulation by PJA2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Rinaldi,L., Chiuso,F., Senatore,E., Borzacchiello,D., Lignitto,L.,
            Iannucci,R., Donne,R.D., Fuggi,M., Reale,C., Russo,F., Russo,N.A.,
            Giurato,G., Rizzo,F., Sellitto,A., Santangelo,M., De Biase,D.,
            Paciello,O., D'Ambrosio,C., Amente,S., Garbi,C., Dalla,E.,
            Scaloni,A., Weisz,A., Ambrosino,C., Insabato,L. and Feliciello,A.
  TITLE     Downregulation of praja2 restrains endocytosis and boosts tyrosine
            kinase receptors in kidney cancer
  JOURNAL   Commun Biol 7 (1), 208 (2024)
   PUBMED   38379085
  REMARK    GeneRIF: Downregulation of praja2 restrains endocytosis and boosts
            tyrosine kinase receptors in kidney cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4856)
  AUTHORS   Senatore,E., Chiuso,F., Rinaldi,L., Intartaglia,D., Delle Donne,R.,
            Pedone,E., Catalanotti,B., Pirone,L., Fiorillo,B., Moraca,F.,
            Giamundo,G., Scala,G., Raffeiner,A., Torres-Quesada,O., Stefan,E.,
            Kwiatkowski,M., van Pijkeren,A., Morleo,M., Franco,B., Garbi,C.,
            Conte,I. and Feliciello,A.
  TITLE     The TBC1D31/praja2 complex controls primary ciliogenesis through
            PKA-directed OFD1 ubiquitylation
  JOURNAL   EMBO J 40 (10), e106503 (2021)
   PUBMED   33934390
REFERENCE   4  (bases 1 to 4856)
  AUTHORS   Zhao,Z., Zhu,L., Xing,Y. and Zhang,Z.
  TITLE     Praja2 suppresses the growth of gastric cancer by ubiquitylation of
            KSR1 and inhibiting MEK-ERK signal pathways
  JOURNAL   Aging (Albany NY) 13 (3), 3886-3897 (2021)
   PUBMED   33461174
  REMARK    GeneRIF: Praja2 suppresses the growth of gastric cancer by
            ubiquitylation of KSR1 and inhibiting MEK-ERK signal pathways.
REFERENCE   5  (bases 1 to 4856)
  AUTHORS   Zhong,J., Wang,H., Chen,W., Sun,Z., Chen,J., Xu,Y., Weng,M.,
            Shi,Q., Ma,D. and Miao,C.
  TITLE     Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1
            macrophage polarization by activating JNK and p38 pathways
  JOURNAL   Cell Death Dis 8 (5), e2763 (2017)
   PUBMED   28471450
  REMARK    Erratum:[Cell Death Dis. 2018 Jul 16;9(8):782. doi:
            10.1038/s41419-018-0828-y.. PMID: 30013073]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4856)
  AUTHORS   Lignitto,L., Carlucci,A., Sepe,M., Stefan,E., Cuomo,O., Nistico,R.,
            Scorziello,A., Savoia,C., Garbi,C., Annunziato,L. and Feliciello,A.
  TITLE     Control of PKA stability and signalling by the RING ligase praja2
  JOURNAL   Nat Cell Biol 13 (4), 412-422 (2011)
   PUBMED   21423175
REFERENCE   7  (bases 1 to 4856)
  AUTHORS   Sasaki,A., Masuda,Y., Iwai,K., Ikeda,K. and Watanabe,K.
  TITLE     A RING finger protein Praja1 regulates Dlx5-dependent transcription
            through its ubiquitin ligase activity for the Dlx/Msx-interacting
            MAGE/Necdin family protein, Dlxin-1
  JOURNAL   J Biol Chem 277 (25), 22541-22546 (2002)
   PUBMED   11959851
REFERENCE   8  (bases 1 to 4856)
  AUTHORS   Yu,P., Chen,Y., Tagle,D.A. and Cai,T.
  TITLE     PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human
            X chromosome gene abundantly expressed in brain
  JOURNAL   Genomics 79 (6), 869-874 (2002)
   PUBMED   12036302
REFERENCE   9  (bases 1 to 4856)
  AUTHORS   Mishra,L., Tully,R.E., Monga,S.P., Yu,P., Cai,T., Makalowski,W.,
            Mezey,E., Pavan,W.J. and Mishra,B.
  TITLE     Praja1, a novel gene encoding a RING-H2 motif in mouse development
  JOURNAL   Oncogene 15 (19), 2361-2368 (1997)
   PUBMED   9393880
REFERENCE   10 (bases 1 to 4856)
  AUTHORS   Nakayama,M., Miyake,T., Gahara,Y., Ohara,O. and Kitamura,T.
  TITLE     A novel RING-H2 motif protein downregulated by axotomy: its
            characteristic localization at the postsynaptic density of
            axosomatic synapse
  JOURNAL   J Neurosci 15 (7 Pt 2), 5238-5248 (1995)
   PUBMED   7623148
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM469517.1, AB007898.1, BQ228553.1 and BP301854.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014819.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB007898.1, BC030826.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000361189.7/ ENSP00000354775.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               BM469517.1         21-123
            104-203             AB007898.1         1-100
            204-785             BQ228553.1         111-692
            786-1109            AB007898.1         683-1006
            1110-1609           BP301854.1         47-546
            1610-4856           AB007898.1         1507-4753
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q21.3"
     gene            1..4856
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /note="praja ring finger ubiquitin ligase 2"
                     /db_xref="GeneID:9867"
                     /db_xref="HGNC:HGNC:17481"
                     /db_xref="MIM:619341"
     exon            1..133
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            134..251
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..2347
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /EC_number="2.3.2.27"
                     /note="praja 2, RING-H2 motif containing; ring finger
                     protein 131; praja2; RING-type E3 ubiquitin transferase
                     Praja-2; praja ring finger 2, E3 ubiquitin protein ligase"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Praja-2"
                     /protein_id="NP_055634.3"
                     /db_xref="CCDS:CCDS4099.1"
                     /db_xref="GeneID:9867"
                     /db_xref="HGNC:HGNC:17481"
                     /db_xref="MIM:619341"
                     /translation="MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVS
                     FKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEI
                     PTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGI
                     ALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPP
                     VPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQE
                     RQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFN
                     RHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENN
                     QMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKD
                     QSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEV
                     NESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAI
                     SYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLED
                     HTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAV
                     IEASAAPSSEPDPDAPPSNDSIAEAP"
     exon            252..452
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            453..1503
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1689
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1872
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1984
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2099
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2221
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     exon            2222..4856
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4835..4840
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /note="hexamer: AATAAA"
     polyA_site      4856
                     /gene="PJA2"
                     /gene_synonym="Neurodap1; RNF131"
                     /note="major polyA site"
ORIGIN      
        1 accgccgccg ccgccgccac agccgccagc cgcttcgcgt tcggcggggg aggagttgga
       61 ggcggagaag aaggcggtgg tggcgggtgg aggttcgagc gctgttctcg ctccggagcc
      121 gctgcacatt tcggaatctt ctgcggcttg tccatagtgt gaataaaaac taaatcacat
      181 ctataattct actgaactgg tcacacagac gctgccatat atgtcacagt acactgaaaa
      241 ggagccagca gcaatggacc aagaatctgg taaggctgtc tggcccaaac cagcaggagg
      301 gtatcagaca attacaggca ggagatatgg aagaagacat gcttatgtca gttttaaacc
      361 atgtatgacc agacatgaaa gaagcttagg tcgggctggt gatgactatg aagtgttgga
      421 actagatgat gttccaaagg aaaattcctc aggttccagt cctttggatc aagttgattc
      481 ttctttaccc agtgaaccta tatttgaaaa aagtgaaaca gaaattccca cttgtggttc
      541 agcattgaat caaaccactg agagcagtca atcctttgtt gcagtacatc acagtgagga
      601 aggcagggat accttaggaa gcagtacaaa tcttcataat cactctgagg gagagtatat
      661 tccaggagct tgtagtgctt caagtgtcca aaatggaatt gcattggttc atacagactc
      721 ttatgatcca gatggcaaac atggagaaga taatgaccat cttcaacttt ctgcagaagt
      781 cgtggaaggt agtagatacc aggaatcatt aggcaataca gtatttgagt tggaaaacag
      841 agaggcagag gcatacactg gtctttcacc accagttccc tcatttaact gtgaagtaag
      901 agatgagttt gaagagttag attctgtacc attagtgaaa agttctgctg gtgatactga
      961 gtttgtccat cagaatagcc aggaaattca gaggtcttct caagatgaaa tggttagtac
     1021 gaaacaacaa aataatacta gccaggaaag acagacagaa cattcacctg aagatgcagc
     1081 ctgtggtcca gggcatattt gtagtgaaca aaataccaat gatagggaaa agaaccatgg
     1141 aagttctcct gaacaggtag tgaggccaaa agttagaaaa ctgataagtt caagccaggt
     1201 ggaccaagaa acaggtttta ataggcatga ggcgaaacaa agaagtgttc aaagatggag
     1261 agaggctttg gaagttgagg aaagtggctc agatgacctc ttaataaaat gtgaagaata
     1321 tgatggagag catgactgta tgttcttgga tccaccatac tcaagagtta ttacacaaag
     1381 ggaaacagaa aataaccaaa tgacatcaga aagtggagcc acagcaggaa ggcaagaggt
     1441 ggataacacc ttttggaatg gctgtggaga ttattaccaa ctctatgaca aagatgaaga
     1501 tagttctgaa tgcagtgatg gggaatggtc tgcttctttg cctcatcgat tttctggtac
     1561 agaaaaagat caatcctcaa gtgatgaaag ctgggagact ctgccaggaa aagatgagaa
     1621 tgaacctgag ctacaaagtg atagcagtgg ccctgaagaa gaaaaccaag aattatctct
     1681 tcaggaaggg gaacagacat ccttggaaga gggagaaatt ccttggttac agtacaatga
     1741 agtcaatgaa agcagcagtg atgagggaaa tgaacctgcc aatgaatttg cacagccagc
     1801 tttcatgttg gatggtaaca ataacctgga ggatgactcc agtgtgagtg aagacttaga
     1861 tgtggattgg agcctatttg atggctttgc agatggacta ggagttgctg aagctatttc
     1921 atatgtggat cctcagttcc ttacctacat ggcactagaa gaacgcttag cccaggctat
     1981 ggagactgct ctggcccatt tagagtctct tgcagtggat gttgaggtgg ccaatccacc
     2041 agctagtaag gaaagcattg atggtcttcc agagaccctt gttcttgaag atcacactgc
     2101 tattggtcag gaacaatgct gtccaatctg ttgcagtgag tatattaagg atgatatagc
     2161 aacagagttg ccctgtcacc atttctttca caaaccttgt gtctcaattt ggctacaaaa
     2221 gtcgggaaca tgccctgtgt gccgccgtca tttcccacct gcggttattg aagcatctgc
     2281 agctccttcc tctgagcctg atcctgatgc cccaccttca aatgacagta ttgcagaagc
     2341 accctaaacc ttgacagttg aaatgagatc agtgtatcaa agtaaatctg caaattcctt
     2401 ctaaatttca tgtgcaaata attatatata aatatattta aaaatgctat atatagtata
     2461 tgccatagtt tagaaagaat attaaccttt ctaaactaaa tttaggtttg cagaaagtat
     2521 taaacatttt taagctgaat gttgagacag tgcatccatt ttctttagtt gaatatgttt
     2581 gtattaattg taaagccaag cttatcagtt gactctctcc agaataaata atcatctgtg
     2641 tggcatacgt tattggcttt gtctgtaata ctgccactaa gtgattataa ttaagctgtc
     2701 ctgtttgcat caaatgtaaa gactgtgttc acaaccgtag taaaatttgg tttcattgga
     2761 aatgaaacaa attctaaagt atgctttttc actagtccct ttgattttgc tatatcataa
     2821 cctcttggtt catatgctga gaaatttttc agaaagtcca tttttgttta aaattagaac
     2881 ttttcagaat gccaaatgaa ggctaaaatt ttggccagaa cattacaaaa gttttaaatc
     2941 gtagacgtaa ctccccctga aataaagtta ggtagtaaaa tccttaatga aaccagtgga
     3001 tgtgcttaac gtaaggttag taaagcatac aaagaatcta gtgtgctcag ggcttggtac
     3061 aatgagctga attagatggc cttatgaaac tctttctaac ctcttaccca acctgtttct
     3121 ccttggttaa aattatactt gaaggcccag aacactcatg gcacatttgt ttaatattgc
     3181 ttatagttag tttaaggtaa ttttgcttct acagtatttt ggaaggtctg aaaacttgca
     3241 cagggtcatc tttgtaatta tataacccca aactaagatg cacaatgtct ccttcaggtg
     3301 atcacacaca gtggacgagt atgtgcaaac atggacataa tagttcactt acaaatgtga
     3361 tttgatgtta acactagaga atgatgactg tagaacattt gagcaagtaa aatagtaaag
     3421 cacatagtga gtgtatgtcc gtctaactgg tacattgata atttagtttg ggcacataaa
     3481 aggaatattt atatggcttc ccaaatgcag agttacatct tattcgtgta tttctctgag
     3541 tatttatatc ccgtctcctt ttttcattct taaaaataaa tgaattttca ctgttggcac
     3601 atatgaggct taaatataag gaacataaca cttgcattct aatttttgca tatattgtaa
     3661 atgtgtctgg tatttacagc aaaatactgt gtatcctttt atgggtaaaa caaaagtgaa
     3721 cattgcatgc atgtaatgtg atgaatttgt aatttaggag ttctttgggg cttctgtgac
     3781 ttgggaaatg cttacattca ggccttaatg ttgcattagc tagcatgttt tccctctgat
     3841 gtatatagtc actgttgtat aaactaatct ttgcttgttt tctactctgt gatctttcca
     3901 tatcatattt cattaatgat cagttagtgt caaggagtca aaacagatta aaattaattt
     3961 catgtgtata tggtggaaat ttgtggctag tgtgattttt gtttgtttcc ttttaagtac
     4021 tgttgatcag ttgtgacact tactggttaa acttacgttg ctaaagattt ctctataata
     4081 agccacacat tatatttaga ctatattaag ggaccttggt tttcttctag atagcagctg
     4141 tcccaaagaa aatatttctt ctttgtctgt taagatttag ctattatctg ccagttgtta
     4201 agaggttttg gttccaaact caaccagcaa tgttgagagc tgaacttaag atagctgttg
     4261 tactttttgc tttccatctg ttactgtcct tcattcttgg ctccctacta tctataaaca
     4321 gctgctgtga agaagaaaag ttgaataaga gttggcttaa attttaaaaa agaaaaagaa
     4381 aattgaggtt ttaggatttt catggtaaca agctctggta taagctaagg ctggcaagtt
     4441 cagatactaa aatattattt gatcatatct tggatccttt tgaaaaagtt aagactatat
     4501 gaaggtaaat tagaaataag tatgaatatt aataaaatag catttatctt atttctctat
     4561 tttatgttgt gacttaacct aattttattt ttttaacatt ttcttatttc ttataatatg
     4621 aatgctgata tttaaaggta gatctatgtg gtattctttg tgtttcttaa ttgtttaact
     4681 cttaagatta tttgtgatct ggatttatgt atttgttaga tacatacgaa ttgttaaaat
     4741 ggaatgcaag tttttcaaaa gcccaggtct aaatgtaatg gttggtttat tgttctataa
     4801 ccccagccca tcattttctg tgtaaatcat aaacaataaa cagaatatac tcggtg
//
