LOCUS       NM_001382776            2356 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1),
            transcript variant 5, mRNA.
ACCESSION   NM_001382776 XM_005262292
VERSION     NM_001382776.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Huang,S.Y., Gong,S., Zhao,Y., Ye,M.L., Li,J.Y., He,Q.M., Qiao,H.,
            Tan,X.R., Wang,J.Y., Liang,Y.L., Huang,S.W., He,S.W., Li,Y.Q.,
            Xu,S., Li,Y.Q. and Liu,N.
  TITLE     PJA1-mediated suppression of pyroptosis as a driver of docetaxel
            resistance in nasopharyngeal carcinoma
  JOURNAL   Nat Commun 15 (1), 5300 (2024)
   PUBMED   38906860
  REMARK    GeneRIF: PJA1-mediated suppression of pyroptosis as a driver of
            docetaxel resistance in nasopharyngeal carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Ghosh,B., Karmakar,S., Prasad,M. and Mandal,A.K.
  TITLE     Praja1 ubiquitin ligase facilitates degradation of polyglutamine
            proteins and suppresses polyglutamine-mediated toxicity
  JOURNAL   Mol Biol Cell 32 (17), 1579-1593 (2021)
   PUBMED   34161122
  REMARK    GeneRIF: Praja1 ubiquitin ligase facilitates degradation of
            polyglutamine proteins and suppresses polyglutamine-mediated
            toxicity.
REFERENCE   3  (bases 1 to 2356)
  AUTHORS   Luo,Z., Ye,X., Cheng,Y., Li,F., Shou,F. and Wang,G.
  TITLE     E3 ubiquitin ligase PJA1 regulates lung adenocarcinoma apoptosis
            and invasion through promoting FOXR2 degradation
  JOURNAL   Biochem Biophys Res Commun 556, 106-113 (2021)
   PUBMED   33839405
  REMARK    GeneRIF: E3 ubiquitin ligase PJA1 regulates lung adenocarcinoma
            apoptosis and invasion through promoting FOXR2 degradation.
REFERENCE   4  (bases 1 to 2356)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2356)
  AUTHORS   Watabe,K., Kato,Y., Sakuma,M., Murata,M., Niida-Kawaguchi,M.,
            Takemura,T., Hanagata,N., Tada,M., Kakita,A. and Shibata,N.
  TITLE     Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal
            cytoplasmic TDP-43 aggregate formation
  JOURNAL   Neuropathology 40 (6), 570-586 (2020)
   PUBMED   32686212
  REMARK    GeneRIF: Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal
            cytoplasmic TDP-43 aggregate formation.
REFERENCE   6  (bases 1 to 2356)
  AUTHORS   Saha,T., Vardhini,D., Tang,Y., Katuri,V., Jogunoori,W., Volpe,E.A.,
            Haines,D., Sidawy,A., Zhou,X., Gallicano,I., Schlegel,R., Mishra,B.
            and Mishra,L.
  TITLE     RING finger-dependent ubiquitination by PRAJA is dependent on
            TGF-beta and potentially defines the functional status of the tumor
            suppressor ELF
  JOURNAL   Oncogene 25 (5), 693-705 (2006)
   PUBMED   16247473
REFERENCE   7  (bases 1 to 2356)
  AUTHORS   Mishra,L., Katuri,V. and Evans,S.
  TITLE     The role of PRAJA and ELF in TGF-beta signaling and gastric cancer
  JOURNAL   Cancer Biol Ther 4 (7), 694-699 (2005)
   PUBMED   16096365
  REMARK    Review article
REFERENCE   8  (bases 1 to 2356)
  AUTHORS   Sasaki,A., Masuda,Y., Iwai,K., Ikeda,K. and Watanabe,K.
  TITLE     A RING finger protein Praja1 regulates Dlx5-dependent transcription
            through its ubiquitin ligase activity for the Dlx/Msx-interacting
            MAGE/Necdin family protein, Dlxin-1
  JOURNAL   J Biol Chem 277 (25), 22541-22546 (2002)
   PUBMED   11959851
  REMARK    GeneRIF: A RING finger protein Praja1 regulates Dlx5-dependent
            transcription through its ubiquitin ligase activity for the
            Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1.
REFERENCE   9  (bases 1 to 2356)
  AUTHORS   Yu,P., Chen,Y., Tagle,D.A. and Cai,T.
  TITLE     PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human
            X chromosome gene abundantly expressed in brain
  JOURNAL   Genomics 79 (6), 869-874 (2002)
   PUBMED   12036302
  REMARK    GeneRIF: Involved in protein ubiquitination in the brain.
            Candidate gene for X-linked mental retardation.
REFERENCE   10 (bases 1 to 2356)
  AUTHORS   Mishra,L., Tully,R.E., Monga,S.P., Yu,P., Cai,T., Makalowski,W.,
            Mezey,E., Pavan,W.J. and Mishra,B.
  TITLE     Praja1, a novel gene encoding a RING-H2 motif in mouse development
  JOURNAL   Oncogene 15 (19), 2361-2368 (1997)
   PUBMED   9393880
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL157699.13.
            
            On May 20, 2020 this sequence version replaced XM_005262292.1.
            
            Summary: This gene encodes an enzyme that has E2-dependent E3
            ubiquitin-protein ligase activity. This enzyme belongs to a class
            of ubiquitin ligases that include a RING finger motif, and it can
            interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene
            is located in an area of chromosome X where several X-linked
            cognitive disability disorders have been associated, and it has
            also been found as part of a contiguous gene deletion associated
            with craniofrontonasal syndrome, though a direct link to any
            disorder has yet to be demonstrated. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, May 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13316199.307129.1,
                                           SRR18074968.2380547.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AL157699.13        84502-84583         c
            83-2356             AL157699.13        79991-82264         c
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.1"
     gene            1..2356
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="praja ring finger ubiquitin ligase 1"
                     /db_xref="GeneID:64219"
                     /db_xref="HGNC:HGNC:16648"
                     /db_xref="MIM:300420"
     exon            1..82
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /inference="alignment:Splign:2.1.0"
     exon            83..2356
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..1910
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /EC_number="2.3.2.27"
                     /note="isoform b is encoded by transcript variant 5; praja
                     1; E3 ubiquitin-protein ligase Praja-1; RING finger
                     protein 70; praja ring finger 1, E3 ubiquitin protein
                     ligase; RING-type E3 ubiquitin transferase Praja-1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Praja-1 isoform b"
                     /protein_id="NP_001369705.1"
                     /db_xref="CCDS:CCDS35316.1"
                     /db_xref="GeneID:64219"
                     /db_xref="HGNC:HGNC:16648"
                     /db_xref="MIM:300420"
                     /translation="MHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRY
                     PPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKG
                     ARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLAT
                     KGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKI
                     FFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSD
                     QVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSS
                     GESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREP
                     SLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSE
                     DLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVE
                     VANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPC
                     VSIWLQKSGTCPVCRCMFPPPL"
     regulatory      2333..2338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="hexamer: ATTAAA"
     polyA_site      2356
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="major polyA site"
ORIGIN      
        1 gggagcagcg gctctggcgg tcgtggcggt cggagatccg agagctctgc ttctctgaaa
       61 agcgtgtggc tttctggact tggccaccca cgagccagcg ggaaaggatt gccagccaga
      121 gaaagacgaa ctccgaagtc ccaatgcaca gatcagcccc cagtcaaacc accaagagga
      181 gccgatcgcc attttccact actcgtcgta gttgggacga cagcgagagt tcgggaacca
      241 acctgaatat tgataatgag gactattcca ggtatccgcc aagagagtac agagcttcgg
      301 gtagcagaag aggaatggcc tatggacata ttgactctta tggggcagat gatagtgagg
      361 aggagggggc tgggcctgtt gagcgaccgc cagtgagagg gaaaactggc aagtttaaag
      421 atgataagct gtatgaccca gagaaagggg caaggtcttt ggctgggcca cctccacatt
      481 tctctagttt tagccgtgat gtgagagagg agcgagacaa gttagaccca gtccctgcag
      541 caagatgctc agctagcaga gctgacttcc tgccacaaag tagtgtggcc tcacagtcgt
      601 cttctgaagg caagctggct acaaaaggtg acagctcgga gagggagaga agggagcaaa
      661 atttacctgc acgtcccagc agggctcctg tgagtatttg tggtggtggg gaaaacacct
      721 caaagagtgc agaggaacct gtggtcaggc ccaaaatcag aaacctggca agtccaaact
      781 gcgtgaaacc aaaaattttt tttgatactg atgatgatga cgatatgcca cacagtactt
      841 ccaggtggag ggataccgcc aatgacaatg agggccactc ggatggcctg gcaagaagag
      901 ggagaggcga gagttcaagt ggctatcccg agccaaagta ccctgaagac aaacgggaag
      961 cgaggagtga ccaagtgaaa ccagaaaagg tgccgagacg acgacgcacc atggccgacc
     1021 ctgacttctg gacgcacagt gatgattact acaaatactg cgacgaagac tctgacagtg
     1081 acaaagagtg gattgctgct ctgcgtcgga aatatcgaag ccgagagcaa accctgtcct
     1141 ccagtggcga aagctgggag actctgccgg ggaaagaaga gcgggaacct ccacaggcta
     1201 aggtgagtgc cagcactggc accagccctg gccccggtgc tagtgccagt gccggggctg
     1261 gcgccggggc cagtgctggc agcaatggca gcaattacct tgaagaagtt cgagaaccat
     1321 ctcttcagga agagcaggca tccctggaag aaggagaaat tccttggctc cagtaccatg
     1381 agaatgacag tagcagtgag ggggataatg attctggtca cgagttgatg caacctgggg
     1441 tattcatgct ggatggaaac aacaaccttg aagatgactc cagtgtgagc gaagacctag
     1501 aagtggattg gagcctcttt gatggatttg cagatgggtt aggagtggct gaagccattt
     1561 cctatgtgga ccctcagttc ctcacctaca tggcacttga agaacgcctg gcccaggcaa
     1621 tggaaactgc ccttgcgcac ttggagtctc tcgcagtgga tgtagaggtg gccaatccac
     1681 cagcaagcaa ggagagcatt gacgctcttc ccgagatcct ggtcactgaa gatcatggcg
     1741 cagttggtca ggagatgtgc tgccccatct gctgtagcga atatgtgaag ggggaggtgg
     1801 caactgagct gccgtgccac cactatttcc acaagccgtg tgtgtccatc tggcttcaga
     1861 agtcaggcac ctgccccgtg tgccgctgca tgttccctcc cccactctaa agaccaaggc
     1921 cgtttactcc tggtctgatt attttcccca tctgaaatcc acaatactgc aggagccctc
     1981 ttgaaattaa caatggaaat aaaaccaatc agtcagttag cctaaaccta ttgattcctc
     2041 gtgattattt ccaatgtgaa aacagttgtg tatgattgca ttaaaaatca tatcatcttt
     2101 tagaggttag aaaagggaaa actaaacttt ctaaatgcta cttgagattg cagtaagaag
     2161 atacgttttc taacctgaaa gttaaatcgc atttgttttc ttcagtagaa tgggaatgtg
     2221 ttgctgttac ttgtaatgtc aagtttatct gttaaatatg tccaaaaggc aaaatcattt
     2281 ttgttgcatg ttatgggtcg atgttcctgt aattgcagtg ccgtaaaagc ttattaaagt
     2341 tgttcttttg gtttta
//
