LOCUS       NM_001382775            2520 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1),
            transcript variant 4, mRNA.
ACCESSION   NM_001382775
VERSION     NM_001382775.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Huang,S.Y., Gong,S., Zhao,Y., Ye,M.L., Li,J.Y., He,Q.M., Qiao,H.,
            Tan,X.R., Wang,J.Y., Liang,Y.L., Huang,S.W., He,S.W., Li,Y.Q.,
            Xu,S., Li,Y.Q. and Liu,N.
  TITLE     PJA1-mediated suppression of pyroptosis as a driver of docetaxel
            resistance in nasopharyngeal carcinoma
  JOURNAL   Nat Commun 15 (1), 5300 (2024)
   PUBMED   38906860
  REMARK    GeneRIF: PJA1-mediated suppression of pyroptosis as a driver of
            docetaxel resistance in nasopharyngeal carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Ghosh,B., Karmakar,S., Prasad,M. and Mandal,A.K.
  TITLE     Praja1 ubiquitin ligase facilitates degradation of polyglutamine
            proteins and suppresses polyglutamine-mediated toxicity
  JOURNAL   Mol Biol Cell 32 (17), 1579-1593 (2021)
   PUBMED   34161122
  REMARK    GeneRIF: Praja1 ubiquitin ligase facilitates degradation of
            polyglutamine proteins and suppresses polyglutamine-mediated
            toxicity.
REFERENCE   3  (bases 1 to 2520)
  AUTHORS   Luo,Z., Ye,X., Cheng,Y., Li,F., Shou,F. and Wang,G.
  TITLE     E3 ubiquitin ligase PJA1 regulates lung adenocarcinoma apoptosis
            and invasion through promoting FOXR2 degradation
  JOURNAL   Biochem Biophys Res Commun 556, 106-113 (2021)
   PUBMED   33839405
  REMARK    GeneRIF: E3 ubiquitin ligase PJA1 regulates lung adenocarcinoma
            apoptosis and invasion through promoting FOXR2 degradation.
REFERENCE   4  (bases 1 to 2520)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2520)
  AUTHORS   Watabe,K., Kato,Y., Sakuma,M., Murata,M., Niida-Kawaguchi,M.,
            Takemura,T., Hanagata,N., Tada,M., Kakita,A. and Shibata,N.
  TITLE     Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal
            cytoplasmic TDP-43 aggregate formation
  JOURNAL   Neuropathology 40 (6), 570-586 (2020)
   PUBMED   32686212
  REMARK    GeneRIF: Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal
            cytoplasmic TDP-43 aggregate formation.
REFERENCE   6  (bases 1 to 2520)
  AUTHORS   Saha,T., Vardhini,D., Tang,Y., Katuri,V., Jogunoori,W., Volpe,E.A.,
            Haines,D., Sidawy,A., Zhou,X., Gallicano,I., Schlegel,R., Mishra,B.
            and Mishra,L.
  TITLE     RING finger-dependent ubiquitination by PRAJA is dependent on
            TGF-beta and potentially defines the functional status of the tumor
            suppressor ELF
  JOURNAL   Oncogene 25 (5), 693-705 (2006)
   PUBMED   16247473
REFERENCE   7  (bases 1 to 2520)
  AUTHORS   Mishra,L., Katuri,V. and Evans,S.
  TITLE     The role of PRAJA and ELF in TGF-beta signaling and gastric cancer
  JOURNAL   Cancer Biol Ther 4 (7), 694-699 (2005)
   PUBMED   16096365
  REMARK    Review article
REFERENCE   8  (bases 1 to 2520)
  AUTHORS   Sasaki,A., Masuda,Y., Iwai,K., Ikeda,K. and Watanabe,K.
  TITLE     A RING finger protein Praja1 regulates Dlx5-dependent transcription
            through its ubiquitin ligase activity for the Dlx/Msx-interacting
            MAGE/Necdin family protein, Dlxin-1
  JOURNAL   J Biol Chem 277 (25), 22541-22546 (2002)
   PUBMED   11959851
  REMARK    GeneRIF: A RING finger protein Praja1 regulates Dlx5-dependent
            transcription through its ubiquitin ligase activity for the
            Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1.
REFERENCE   9  (bases 1 to 2520)
  AUTHORS   Yu,P., Chen,Y., Tagle,D.A. and Cai,T.
  TITLE     PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human
            X chromosome gene abundantly expressed in brain
  JOURNAL   Genomics 79 (6), 869-874 (2002)
   PUBMED   12036302
  REMARK    GeneRIF: Involved in protein ubiquitination in the brain.
            Candidate gene for X-linked mental retardation.
REFERENCE   10 (bases 1 to 2520)
  AUTHORS   Mishra,L., Tully,R.E., Monga,S.P., Yu,P., Cai,T., Makalowski,W.,
            Mezey,E., Pavan,W.J. and Mishra,B.
  TITLE     Praja1, a novel gene encoding a RING-H2 motif in mouse development
  JOURNAL   Oncogene 15 (19), 2361-2368 (1997)
   PUBMED   9393880
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL157699.13.
            
            Summary: This gene encodes an enzyme that has E2-dependent E3
            ubiquitin-protein ligase activity. This enzyme belongs to a class
            of ubiquitin ligases that include a RING finger motif, and it can
            interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene
            is located in an area of chromosome X where several X-linked
            cognitive disability disorders have been associated, and it has
            also been found as part of a contiguous gene deletion associated
            with craniofrontonasal syndrome, though a direct link to any
            disorder has yet to be demonstrated. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, May 2010].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.29544.1,
                                           SRR1803612.13073.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AL157699.13        84502-84583         c
            83-2520             AL157699.13        79991-82428         c
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.1"
     gene            1..2520
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="praja ring finger ubiquitin ligase 1"
                     /db_xref="GeneID:64219"
                     /db_xref="HGNC:HGNC:16648"
                     /db_xref="MIM:300420"
     exon            1..82
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /inference="alignment:Splign:2.1.0"
     exon            83..2520
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..2074
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 4; praja
                     1; E3 ubiquitin-protein ligase Praja-1; RING finger
                     protein 70; praja ring finger 1, E3 ubiquitin protein
                     ligase; RING-type E3 ubiquitin transferase Praja-1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Praja-1 isoform a"
                     /protein_id="NP_001369704.1"
                     /db_xref="CCDS:CCDS14393.1"
                     /db_xref="GeneID:64219"
                     /db_xref="HGNC:HGNC:16648"
                     /db_xref="MIM:300420"
                     /translation="MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERI
                     ASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPR
                     EYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARS
                     LAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGD
                     SSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFD
                     TDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVK
                     PEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGES
                     WETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQ
                     EEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLE
                     VDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVAN
                     PPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSI
                     WLQKSGTCPVCRCMFPPPL"
     regulatory      2497..2502
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="hexamer: ATTAAA"
     polyA_site      2520
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="major polyA site"
ORIGIN      
        1 gggagcagcg gctctggcgg tcgtggcggt cggagatccg agagctctgc ttctctgaaa
       61 agcgtgtggc tttctggact tgggagcagg agccctccta cccgagcccc agctgtattg
      121 gaaaccctcc taagtcagag tgatgggtca ggaatctagc aagcctgtat ggcccaatcc
      181 aacaggaggg tatcagtcca atacaggtag gaggtatgga agaaggcatg cttatgtcag
      241 tttcaggcca cccacgagcc agcgggaaag gattgccagc cagagaaaga cgaactccga
      301 agtcccaatg cacagatcag cccccagtca aaccaccaag aggagccgat cgccattttc
      361 cactactcgt cgtagttggg acgacagcga gagttcggga accaacctga atattgataa
      421 tgaggactat tccaggtatc cgccaagaga gtacagagct tcgggtagca gaagaggaat
      481 ggcctatgga catattgact cttatggggc agatgatagt gaggaggagg gggctgggcc
      541 tgttgagcga ccgccagtga gagggaaaac tggcaagttt aaagatgata agctgtatga
      601 cccagagaaa ggggcaaggt ctttggctgg gccacctcca catttctcta gttttagccg
      661 tgatgtgaga gaggagcgag acaagttaga cccagtccct gcagcaagat gctcagctag
      721 cagagctgac ttcctgccac aaagtagtgt ggcctcacag tcgtcttctg aaggcaagct
      781 ggctacaaaa ggtgacagct cggagaggga gagaagggag caaaatttac ctgcacgtcc
      841 cagcagggct cctgtgagta tttgtggtgg tggggaaaac acctcaaaga gtgcagagga
      901 acctgtggtc aggcccaaaa tcagaaacct ggcaagtcca aactgcgtga aaccaaaaat
      961 tttttttgat actgatgatg atgacgatat gccacacagt acttccaggt ggagggatac
     1021 cgccaatgac aatgagggcc actcggatgg cctggcaaga agagggagag gcgagagttc
     1081 aagtggctat cccgagccaa agtaccctga agacaaacgg gaagcgagga gtgaccaagt
     1141 gaaaccagaa aaggtgccga gacgacgacg caccatggcc gaccctgact tctggacgca
     1201 cagtgatgat tactacaaat actgcgacga agactctgac agtgacaaag agtggattgc
     1261 tgctctgcgt cggaaatatc gaagccgaga gcaaaccctg tcctccagtg gcgaaagctg
     1321 ggagactctg ccggggaaag aagagcggga acctccacag gctaaggtga gtgccagcac
     1381 tggcaccagc cctggccccg gtgctagtgc cagtgccggg gctggcgccg gggccagtgc
     1441 tggcagcaat ggcagcaatt accttgaaga agttcgagaa ccatctcttc aggaagagca
     1501 ggcatccctg gaagaaggag aaattccttg gctccagtac catgagaatg acagtagcag
     1561 tgagggggat aatgattctg gtcacgagtt gatgcaacct ggggtattca tgctggatgg
     1621 aaacaacaac cttgaagatg actccagtgt gagcgaagac ctagaagtgg attggagcct
     1681 ctttgatgga tttgcagatg ggttaggagt ggctgaagcc atttcctatg tggaccctca
     1741 gttcctcacc tacatggcac ttgaagaacg cctggcccag gcaatggaaa ctgcccttgc
     1801 gcacttggag tctctcgcag tggatgtaga ggtggccaat ccaccagcaa gcaaggagag
     1861 cattgacgct cttcccgaga tcctggtcac tgaagatcat ggcgcagttg gtcaggagat
     1921 gtgctgcccc atctgctgta gcgaatatgt gaagggggag gtggcaactg agctgccgtg
     1981 ccaccactat ttccacaagc cgtgtgtgtc catctggctt cagaagtcag gcacctgccc
     2041 cgtgtgccgc tgcatgttcc ctcccccact ctaaagacca aggccgttta ctcctggtct
     2101 gattattttc cccatctgaa atccacaata ctgcaggagc cctcttgaaa ttaacaatgg
     2161 aaataaaacc aatcagtcag ttagcctaaa cctattgatt cctcgtgatt atttccaatg
     2221 tgaaaacagt tgtgtatgat tgcattaaaa atcatatcat cttttagagg ttagaaaagg
     2281 gaaaactaaa ctttctaaat gctacttgag attgcagtaa gaagatacgt tttctaacct
     2341 gaaagttaaa tcgcatttgt tttcttcagt agaatgggaa tgtgttgctg ttacttgtaa
     2401 tgtcaagttt atctgttaaa tatgtccaaa aggcaaaatc atttttgttg catgttatgg
     2461 gtcgatgttc ctgtaattgc agtgccgtaa aagcttatta aagttgttct tttggtttta
//
