LOCUS       NM_001032396            2362 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1),
            transcript variant 2, mRNA.
ACCESSION   NM_001032396
VERSION     NM_001032396.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Huang,S.Y., Gong,S., Zhao,Y., Ye,M.L., Li,J.Y., He,Q.M., Qiao,H.,
            Tan,X.R., Wang,J.Y., Liang,Y.L., Huang,S.W., He,S.W., Li,Y.Q.,
            Xu,S., Li,Y.Q. and Liu,N.
  TITLE     PJA1-mediated suppression of pyroptosis as a driver of docetaxel
            resistance in nasopharyngeal carcinoma
  JOURNAL   Nat Commun 15 (1), 5300 (2024)
   PUBMED   38906860
  REMARK    GeneRIF: PJA1-mediated suppression of pyroptosis as a driver of
            docetaxel resistance in nasopharyngeal carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Ghosh,B., Karmakar,S., Prasad,M. and Mandal,A.K.
  TITLE     Praja1 ubiquitin ligase facilitates degradation of polyglutamine
            proteins and suppresses polyglutamine-mediated toxicity
  JOURNAL   Mol Biol Cell 32 (17), 1579-1593 (2021)
   PUBMED   34161122
  REMARK    GeneRIF: Praja1 ubiquitin ligase facilitates degradation of
            polyglutamine proteins and suppresses polyglutamine-mediated
            toxicity.
REFERENCE   3  (bases 1 to 2362)
  AUTHORS   Luo,Z., Ye,X., Cheng,Y., Li,F., Shou,F. and Wang,G.
  TITLE     E3 ubiquitin ligase PJA1 regulates lung adenocarcinoma apoptosis
            and invasion through promoting FOXR2 degradation
  JOURNAL   Biochem Biophys Res Commun 556, 106-113 (2021)
   PUBMED   33839405
  REMARK    GeneRIF: E3 ubiquitin ligase PJA1 regulates lung adenocarcinoma
            apoptosis and invasion through promoting FOXR2 degradation.
REFERENCE   4  (bases 1 to 2362)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 2362)
  AUTHORS   Watabe,K., Kato,Y., Sakuma,M., Murata,M., Niida-Kawaguchi,M.,
            Takemura,T., Hanagata,N., Tada,M., Kakita,A. and Shibata,N.
  TITLE     Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal
            cytoplasmic TDP-43 aggregate formation
  JOURNAL   Neuropathology 40 (6), 570-586 (2020)
   PUBMED   32686212
  REMARK    GeneRIF: Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal
            cytoplasmic TDP-43 aggregate formation.
REFERENCE   6  (bases 1 to 2362)
  AUTHORS   Saha,T., Vardhini,D., Tang,Y., Katuri,V., Jogunoori,W., Volpe,E.A.,
            Haines,D., Sidawy,A., Zhou,X., Gallicano,I., Schlegel,R., Mishra,B.
            and Mishra,L.
  TITLE     RING finger-dependent ubiquitination by PRAJA is dependent on
            TGF-beta and potentially defines the functional status of the tumor
            suppressor ELF
  JOURNAL   Oncogene 25 (5), 693-705 (2006)
   PUBMED   16247473
REFERENCE   7  (bases 1 to 2362)
  AUTHORS   Mishra,L., Katuri,V. and Evans,S.
  TITLE     The role of PRAJA and ELF in TGF-beta signaling and gastric cancer
  JOURNAL   Cancer Biol Ther 4 (7), 694-699 (2005)
   PUBMED   16096365
  REMARK    Review article
REFERENCE   8  (bases 1 to 2362)
  AUTHORS   Sasaki,A., Masuda,Y., Iwai,K., Ikeda,K. and Watanabe,K.
  TITLE     A RING finger protein Praja1 regulates Dlx5-dependent transcription
            through its ubiquitin ligase activity for the Dlx/Msx-interacting
            MAGE/Necdin family protein, Dlxin-1
  JOURNAL   J Biol Chem 277 (25), 22541-22546 (2002)
   PUBMED   11959851
  REMARK    GeneRIF: A RING finger protein Praja1 regulates Dlx5-dependent
            transcription through its ubiquitin ligase activity for the
            Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1.
REFERENCE   9  (bases 1 to 2362)
  AUTHORS   Yu,P., Chen,Y., Tagle,D.A. and Cai,T.
  TITLE     PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human
            X chromosome gene abundantly expressed in brain
  JOURNAL   Genomics 79 (6), 869-874 (2002)
   PUBMED   12036302
  REMARK    GeneRIF: Involved in protein ubiquitination in the brain.
            Candidate gene for X-linked mental retardation.
REFERENCE   10 (bases 1 to 2362)
  AUTHORS   Mishra,L., Tully,R.E., Monga,S.P., Yu,P., Cai,T., Makalowski,W.,
            Mezey,E., Pavan,W.J. and Mishra,B.
  TITLE     Praja1, a novel gene encoding a RING-H2 motif in mouse development
  JOURNAL   Oncogene 15 (19), 2361-2368 (1997)
   PUBMED   9393880
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC349747.1, AK226171.1 and
            BM127146.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 20, 2020 this sequence version replaced NM_001032396.3.
            
            Summary: This gene encodes an enzyme that has E2-dependent E3
            ubiquitin-protein ligase activity. This enzyme belongs to a class
            of ubiquitin ligases that include a RING finger motif, and it can
            interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene
            is located in an area of chromosome X where several X-linked
            cognitive disability disorders have been associated, and it has
            also been found as part of a contiguous gene deletion associated
            with craniofrontonasal syndrome, though a direct link to any
            disorder has yet to be demonstrated. Alternative splicing results
            in multiple transcript variants. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (2, also known as PJA1beta) lacks
            a portion of the 5' UTR and 5' coding region, and uses a downstream
            start codon, compared to variant 1. The encoded isoform (b) is
            shorter at the N-terminus, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK226171.1, SRR14038192.2972875.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2147920
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374571.5/ ENSP00000363699.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                DC349747.1         341-364
            25-2029             AK226171.1         1-2005
            2030-2362           BM127146.1         124-456             c
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.1"
     gene            1..2362
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="praja ring finger ubiquitin ligase 1"
                     /db_xref="GeneID:64219"
                     /db_xref="HGNC:HGNC:16648"
                     /db_xref="MIM:300420"
     exon            1..82
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /inference="alignment:Splign:2.1.0"
     exon            83..2362
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1916
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /EC_number="2.3.2.27"
                     /note="isoform b is encoded by transcript variant 2; praja
                     1; E3 ubiquitin-protein ligase Praja-1; RING finger
                     protein 70; praja ring finger 1, E3 ubiquitin protein
                     ligase; RING-type E3 ubiquitin transferase Praja-1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Praja-1 isoform b"
                     /protein_id="NP_001027568.1"
                     /db_xref="CCDS:CCDS35316.1"
                     /db_xref="GeneID:64219"
                     /db_xref="HGNC:HGNC:16648"
                     /db_xref="MIM:300420"
                     /translation="MHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRY
                     PPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKG
                     ARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLAT
                     KGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKI
                     FFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSD
                     QVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSS
                     GESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREP
                     SLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSE
                     DLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVE
                     VANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPC
                     VSIWLQKSGTCPVCRCMFPPPL"
     regulatory      2339..2344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="hexamer: ATTAAA"
     polyA_site      2362
                     /gene="PJA1"
                     /gene_synonym="PRAJA1; RNF70"
                     /note="major polyA site"
ORIGIN      
        1 gggagcagcg gctctggcgg tcgtggcggt cggagatccg agagctctgc ttctctgaaa
       61 agcgtgtggc tttctggact tgtttcaggc cacccacgag ccagcgggaa aggattgcca
      121 gccagagaaa gacgaactcc gaagtcccaa tgcacagatc agcccccagt caaaccacca
      181 agaggagccg atcgccattt tccactactc gtcgtagttg ggacgacagc gagagttcgg
      241 gaaccaacct gaatattgat aatgaggact attccaggta tccgccaaga gagtacagag
      301 cttcgggtag cagaagagga atggcctatg gacatattga ctcttatggg gcagatgata
      361 gtgaggagga gggggctggg cctgttgagc gaccgccagt gagagggaaa actggcaagt
      421 ttaaagatga taagctgtat gacccagaga aaggggcaag gtctttggct gggccacctc
      481 cacatttctc tagttttagc cgtgatgtga gagaggagcg agacaagtta gacccagtcc
      541 ctgcagcaag atgctcagct agcagagctg acttcctgcc acaaagtagt gtggcctcac
      601 agtcgtcttc tgaaggcaag ctggctacaa aaggtgacag ctcggagagg gagagaaggg
      661 agcaaaattt acctgcacgt cccagcaggg ctcctgtgag tatttgtggt ggtggggaaa
      721 acacctcaaa gagtgcagag gaacctgtgg tcaggcccaa aatcagaaac ctggcaagtc
      781 caaactgcgt gaaaccaaaa attttttttg atactgatga tgatgacgat atgccacaca
      841 gtacttccag gtggagggat accgccaatg acaatgaggg ccactcggat ggcctggcaa
      901 gaagagggag aggcgagagt tcaagtggct atcccgagcc aaagtaccct gaagacaaac
      961 gggaagcgag gagtgaccaa gtgaaaccag aaaaggtgcc gagacgacga cgcaccatgg
     1021 ccgaccctga cttctggacg cacagtgatg attactacaa atactgcgac gaagactctg
     1081 acagtgacaa agagtggatt gctgctctgc gtcggaaata tcgaagccga gagcaaaccc
     1141 tgtcctccag tggcgaaagc tgggagactc tgccggggaa agaagagcgg gaacctccac
     1201 aggctaaggt gagtgccagc actggcacca gccctggccc cggtgctagt gccagtgccg
     1261 gggctggcgc cggggccagt gctggcagca atggcagcaa ttaccttgaa gaagttcgag
     1321 aaccatctct tcaggaagag caggcatccc tggaagaagg agaaattcct tggctccagt
     1381 accatgagaa tgacagtagc agtgaggggg ataatgattc tggtcacgag ttgatgcaac
     1441 ctggggtatt catgctggat ggaaacaaca accttgaaga tgactccagt gtgagcgaag
     1501 acctagaagt ggattggagc ctctttgatg gatttgcaga tgggttagga gtggctgaag
     1561 ccatttccta tgtggaccct cagttcctca cctacatggc acttgaagaa cgcctggccc
     1621 aggcaatgga aactgccctt gcgcacttgg agtctctcgc agtggatgta gaggtggcca
     1681 atccaccagc aagcaaggag agcattgacg ctcttcccga gatcctggtc actgaagatc
     1741 atggcgcagt tggtcaggag atgtgctgcc ccatctgctg tagcgaatat gtgaaggggg
     1801 aggtggcaac tgagctgccg tgccaccact atttccacaa gccgtgtgtg tccatctggc
     1861 ttcagaagtc aggcacctgc cccgtgtgcc gctgcatgtt ccctccccca ctctaaagac
     1921 caaggccgtt tactcctggt ctgattattt tccccatctg aaatccacaa tactgcagga
     1981 gccctcttga aattaacaat ggaaataaaa ccaatcagtc agttagccta aacctattga
     2041 ttcctcgtga ttatttccaa tgtgaaaaca gttgtgtatg attgcattaa aaatcatatc
     2101 atcttttaga ggttagaaaa gggaaaacta aactttctaa atgctacttg agattgcagt
     2161 aagaagatac gttttctaac ctgaaagtta aatcgcattt gttttcttca gtagaatggg
     2221 aatgtgttgc tgttacttgt aatgtcaagt ttatctgtta aatatgtcca aaaggcaaaa
     2281 tcatttttgt tgcatgttat gggtcgatgt tcctgtaatt gcagtgccgt aaaagcttat
     2341 taaagttgtt cttttggttt ta
//
