LOCUS       NM_152431               3139 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens piwi like RNA-mediated gene silencing 4 (PIWIL4),
            mRNA.
ACCESSION   NM_152431
VERSION     NM_152431.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Bamezai,S., Pulikkottil,A.J., Yadav,T., Vegi,N.M., Mueller,J.,
            Mark,J., Mandal,T., Feder,K., Ihme,S., Song,C., Rosler,R.,
            Wiese,S., Hoell,J.I., Kloetgen,A., Karsan,A., Kumari,A.,
            Wojenski,L., Sinha,A.U., Gonzalez-Menendez,I.,
            Quintanilla-Martinez,L., Donato,E., Trumpp,A., Kruse,E.,
            Hamperl,S., Zou,L., Rawat,V.P.S. and Buske,C.
  TITLE     A noncanonical enzymatic function of PIWIL4 maintains genomic
            integrity and leukemic growth in AML
  JOURNAL   Blood 142 (1), 90-105 (2023)
   PUBMED   37146239
  REMARK    GeneRIF: A noncanonical enzymatic function of PIWIL4 maintains
            genomic integrity and leukemic growth in AML.
            Erratum:[Blood. 2024 Jan 11;143(2):184. doi:
            10.1182/blood.2023023247.. PMID: 38206638]
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Das,B., Sahoo,S. and Mallick,B.
  TITLE     HIWI2 induces G2/M cell cycle arrest and apoptosis in human
            fibrosarcoma via the ROS/DNA damage/p53 axis
  JOURNAL   Life Sci 293, 120353 (2022)
   PUBMED   35074406
  REMARK    GeneRIF: HIWI2 induces G2/M cell cycle arrest and apoptosis in
            human fibrosarcoma via the ROS/DNA damage/p53 axis.
REFERENCE   3  (bases 1 to 3139)
  AUTHORS   Subhramanyam,C.S., Cao,Q., Wang,C., Heng,Z.S.L., Zhou,Z. and Hu,Q.
  TITLE     Role of PIWI-like 4 in modulating neuronal differentiation from
            human embryonal carcinoma cells
  JOURNAL   RNA Biol 17 (11), 1613-1624 (2020)
   PUBMED   32372724
  REMARK    GeneRIF: Role of PIWI-like 4 in modulating neuronal differentiation
            from human embryonal carcinoma cells.
REFERENCE   4  (bases 1 to 3139)
  AUTHORS   He,Z., Jing,S., Yang,T., Chen,J., Huang,F., Zhang,W., Peng,Z.,
            Liu,B., Ma,X., Wu,L., Pan,T., Zhang,X., Li,L., Cai,W., Tang,X.,
            Zhang,J. and Zhang,H.
  TITLE     PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically
            Suppressive Modifications on the 5' Long Terminal Repeat
  JOURNAL   J Virol 94 (10), e01923-19 (2020)
   PUBMED   32161174
  REMARK    GeneRIF: PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically
            Suppressive Modifications on the 5' Long Terminal Repeat.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3139)
  AUTHORS   Heng,Z.S.L., Lee,J.Y., Subhramanyam,C.S., Wang,C., Thanga,L.Z. and
            Hu,Q.
  TITLE     The role of 17beta-estradiol-induced upregulation of Piwi-like 4 in
            modulating gene expression and motility in breast cancer cells
  JOURNAL   Oncol Rep 40 (5), 2525-2535 (2018)
   PUBMED   30226541
  REMARK    GeneRIF: these findings revealed that Piwil4 is a novel regulator
            of ER signaling that could be targeted to inhibit breast cancer
            growth and migration.
REFERENCE   6  (bases 1 to 3139)
  AUTHORS   Sonuga-Barke,E.J., Lasky-Su,J., Neale,B.M., Oades,R., Chen,W.,
            Franke,B., Buitelaar,J., Banaschewski,T., Ebstein,R., Gill,M.,
            Anney,R., Miranda,A., Mulas,F., Roeyers,H., Rothenberger,A.,
            Sergeant,J., Steinhausen,H.C., Thompson,M., Asherson,P. and
            Faraone,S.V.
  TITLE     Does parental expressed emotion moderate genetic effects in ADHD?
            An exploration using a genome wide association scan
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 147B (8), 1359-1368 (2008)
   PUBMED   18846501
REFERENCE   7  (bases 1 to 3139)
  AUTHORS   Sugimoto,K., Kage,H., Aki,N., Sano,A., Kitagawa,H., Nagase,T.,
            Yatomi,Y., Ohishi,N. and Takai,D.
  TITLE     The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus
  JOURNAL   Biochem Biophys Res Commun 359 (3), 497-502 (2007)
   PUBMED   17544373
  REMARK    GeneRIF: PIWIL4 plays important roles in the chromatin-modifying
            pathway in human somatic cells
REFERENCE   8  (bases 1 to 3139)
  AUTHORS   Tahbaz,N., Kolb,F.A., Zhang,H., Jaronczyk,K., Filipowicz,W. and
            Hobman,T.C.
  TITLE     Characterization of the interactions between mammalian PAZ PIWI
            domain proteins and Dicer
  JOURNAL   EMBO Rep 5 (2), 189-194 (2004)
   PUBMED   14749716
REFERENCE   9  (bases 1 to 3139)
  AUTHORS   Sasaki,T., Shiohama,A., Minoshima,S. and Shimizu,N.
  TITLE     Identification of eight members of the Argonaute family in the
            human genome
  JOURNAL   Genomics 82 (3), 323-330 (2003)
   PUBMED   12906857
REFERENCE   10 (bases 1 to 3139)
  AUTHORS   Carmell,M.A., Xuan,Z., Zhang,M.Q. and Hannon,G.J.
  TITLE     The Argonaute family: tentacles that reach into RNAi, developmental
            control, stem cell maintenance, and tumorigenesis
  JOURNAL   Genes Dev 16 (21), 2733-2742 (2002)
   PUBMED   12414724
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB091271.1, BC031060.1 and AB079366.1.
            
            On Nov 22, 2018 this sequence version replaced NM_152431.2.
            
            Summary: PIWIL4 belongs to the Argonaute family of proteins, which
            function in development and maintenance of germline stem cells
            (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005708.1, AB079366.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299001.11/ ENSP00000299001.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               DB091271.1         61-208
            149-2070            BC031060.1         1-1922
            2071-3138           AB079366.1         2091-3158
            3139-3139           BC031060.1         2991-2991
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q21"
     gene            1..3139
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /note="piwi like RNA-mediated gene silencing 4"
                     /db_xref="GeneID:143689"
                     /db_xref="HGNC:HGNC:18444"
                     /db_xref="MIM:610315"
     exon            1..238
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..2710
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /note="piwi-like 4; testis tissue sperm-binding protein Li
                     85P"
                     /codon_start=1
                     /product="piwi-like protein 4"
                     /protein_id="NP_689644.2"
                     /db_xref="CCDS:CCDS31656.1"
                     /db_xref="GeneID:143689"
                     /db_xref="HGNC:HGNC:18444"
                     /db_xref="MIM:610315"
                     /translation="MSGRARVKARGIARSPSATEVGRIQASPLPRSVDLSNNEASSSN
                     GFLGTSRISTNDKYGISSGDAGSTFMERGVKNKQDFMDLSICTREKLAHVRNCKTGSS
                     GIPVKLVTNLFNLDFPQDWQLYQYHVTYIPDLASRRLRIALLYSHSELSNKAKAFDGA
                     ILFLSQKLEEKVTELSSETQRGETIKMTITLKRELPSSSPVCIQVFNIIFRKILKKLS
                     MYQIGRNFYNPSEPMEIPQHKLSLWPGFAISVSYFERKLLFSADVSYKVLRNETVLEF
                     MTALCQRTGLSCFTQTCEKQLIGLIVLTRYNNRTYSIDDIDWSVKPTHTFQKRDGTEI
                     TYVDYYKQQYDITVSDLNQPMLVSLLKKKRNDNSEAQLAHLIPELCFLTGLTDQATSD
                     FQLMKAVAEKTRLSPSGRQQRLARLVDNIQRNTNARFELETWGLHFGSQISLTGRIVP
                     SEKILMQDHICQPVSAADWSKDIRTCKILNAQSLNTWLILCSDRTEYVAESFLNCLRR
                     VAGSMGFNVDYPKIIKVQENPAAFVRAIQQYVDPDVQLVMCILPSNQKTYYDSIKKYL
                     SSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDV
                     CKDALSKDVMVVGCVASVNPRITRWFSRCILQRTMTDVADCLKVFMTGALNKWYKYNH
                     DLPARIIVYRAGVGDGQLKTLIEYEVPQLLSSVAESSSNTSSRLSVIVVRKKCMPRFF
                     TEMNRTVQNPPLGTVVDSEATRNEWYDFYLISQVACRGTVSPTYYNVIYDDNGLKPDH
                     MQRLTFKLCHLYYNWPGIVSVPAPCQYAHKLTFLVAQSIHKEPSLELANHLFYL"
     exon            239..317
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            318..449
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            450..664
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            665..786
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            787..867
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1065
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1177
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1301
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1419
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1531
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1716
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1789
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1990
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2094
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2165
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2319
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2445
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2593
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     exon            2594..3139
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3121..3126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /note="hexamer: AATAAA"
     polyA_site      3139
                     /gene="PIWIL4"
                     /gene_synonym="HIWI2; MIWI2"
                     /note="major polyA site"
ORIGIN      
        1 gcatttccag ccccggcgtt ggttgtggat gctggacatc caccgcctcc aggcagtttc
       61 gccgtcacac cgtcgccatc tgtagccaaa gcaaaacata tcctaactga gactttgcag
      121 ctcttgtggc cactctgggc tcaccgggaa catgagtgga agagcccgag tgaaggccag
      181 aggcatcgcc cgcagcccca gtgccacaga agtggggcgc atccaagcct cgccattgcc
      241 tagatctgtt gatcttagta acaatgaagc atcctctagc aatggcttct tgggaacaag
      301 caggatctca accaacgata aatatgggat atcttctggt gatgctggaa gtaccttcat
      361 ggaaagaggt gtgaaaaaca aacaggactt tatggatttg agtatctgta ccagagaaaa
      421 attggcacat gtgagaaatt gtaaaacagg ttccagtgga atacctgtga aactggttac
      481 aaacctcttt aacttagatt ttccccaaga ctggcagcta taccagtacc atgtgacata
      541 tattccagat ttagcatcta gaaggctgag aattgcttta ctttatagtc atagtgaact
      601 ttccaacaaa gcaaaagcat tcgacggtgc catccttttt ctgtcacaaa agctagaaga
      661 aaaggtcaca gagttgtcaa gtgaaactca aagaggtgag actataaaga tgactatcac
      721 cctgaagagg gagctgccat caagttctcc cgtgtgcatc caggtcttca atatcatctt
      781 cagaaagatc ctcaaaaagt tgtccatgta ccaaattgga cggaacttct ataatccttc
      841 agagccaatg gaaattcccc agcacaaatt atccctttgg cctgggtttg ccatttctgt
      901 gtcatatttt gaaaggaagc tcctgtttag tgctgatgtg agttacaaag tcctccggaa
      961 tgagacggtt ctggaattca tgactgctct ctgtcaaaga actggcttgt cctgtttcac
     1021 ccagacgtgt gagaagcagc taatagggct cattgtcctt acaagataca ataacagaac
     1081 ctactccatt gatgacattg actggtcagt gaagcccaca cacacctttc agaagcggga
     1141 tggcaccgag atcacctatg tggattacta caagcagcag tatgatatta ctgtatcgga
     1201 cctgaatcag cccatgcttg ttagtctgtt aaagaagaag agaaatgaca acagtgaggc
     1261 tcagctcgcc cacctgatac ctgagctctg ctttctaaca gggctgactg accaggcaac
     1321 atctgatttc cagctgatga aggctgtggc tgaaaagaca cgtctcagtc cttcaggccg
     1381 gcagcagcgc ctggccaggc ttgtggacaa catccagagg aataccaatg ctcgctttga
     1441 actagagacc tggggactgc attttggaag ccagatatct ctgactggcc ggattgtgcc
     1501 ttcagaaaaa atattaatgc aagaccacat atgtcaacct gtgtctgctg ctgactggtc
     1561 caaggatatt cgaacttgca agattttaaa tgcacagtct ttgaatacct ggttgatttt
     1621 atgtagcgac agaactgaat atgttgccga gagctttctg aactgcttga gaagagttgc
     1681 aggttccatg ggatttaatg tggactaccc caaaatcata aaagtacaag aaaatccagc
     1741 tgcatttgtt agagctatac agcaatatgt tgatcctgat gttcagctgg taatgtgcat
     1801 tctgccttct aatcagaaga cctattatga ttccattaaa aaatatttga gctcagactg
     1861 cccagtccca agccaatgtg tgcttgctcg gaccttgaat aaacagggca tgatgatgag
     1921 tatcgccacc aagatcgcta tgcagatgac ttgcaagctc ggaggcgagc tgtgggctgt
     1981 ggaaatacct ttaaagtccc tgatggtggt cggtattgat gtctgtaaag atgcactcag
     2041 caaggacgtg atggttgttg gatgcgtggc cagtgttaac cccagaatca ccaggtggtt
     2101 ttcccgctgt atccttcaga gaacaatgac tgatgttgca gattgcttga aagttttcat
     2161 gactggagca ctcaacaaat ggtacaagta caatcatgat ttgccagcac ggataattgt
     2221 gtaccgtgct ggtgtagggg atggtcagct gaaaacactt attgaatatg aagtcccaca
     2281 gctgctgagc agtgtggcag aatccagctc aaataccagc tcaagactgt cggtgattgt
     2341 ggtcaggaag aagtgcatgc cacgattctt taccgaaatg aaccgcactg tacagaaccc
     2401 cccacttggc actgttgtgg attcagaagc aacacgtaac gaatggtatg acttttatct
     2461 gatcagccag gtggcctgcc ggggaactgt tagtcctacc tactataatg tcatctatga
     2521 tgacaacggc ttgaagcccg accatatgca gagacttaca ttcaaattgt gccacctgta
     2581 ctacaactgg ccgggcatag tcagtgtccc agcaccatgt cagtatgctc acaagctgac
     2641 ctttctggtg gcacaaagca ttcataaaga acccagtctg gaattagcca accatctctt
     2701 ctacctgtga tggcatgaac tactggcatc actagatgga caatccaaga agaaattggt
     2761 atactttgtg caaatctgcc ataagctcaa ggctgtgact ggggaaaaag attgagctta
     2821 gttttcatgt ctaggaaaaa aagcaaaaca acttaatctg aaacagtttt aaaaaatgtg
     2881 tgttattttg ttttaaagag ttgtatgctt ggggtaaatt ttcattgtca tatgtggaat
     2941 ttaaatatac catcatctac aaagaattcc acagagttaa atatcttaag ttaaacactt
     3001 aaaataagtg tttgcgtgat attttgatga cagataaaca gagtctaatt cccaccccaa
     3061 attttgctga ggttttctta atgttgtaga gcattttgta gagtggttta aatagttgaa
     3121 aataaagttc agaacatca
//
