LOCUS       NM_018068               5114 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens piwi like RNA-mediated gene silencing 2 (PIWIL2),
            transcript variant 2, mRNA.
ACCESSION   NM_018068 XM_938848 XM_943905
VERSION     NM_018068.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Lu,X., Zhu,Q., Du,H., Gu,M. and Li,X.
  TITLE     PIWIL2 restrains the progression of thyroid cancer via interaction
            with miR-146a-3p
  JOURNAL   BMC Endocr Disord 23 (1), 184 (2023)
   PUBMED   37641092
  REMARK    GeneRIF: PIWIL2 restrains the progression of thyroid cancer via
            interaction with miR-146a-3p.
            Erratum:[BMC Endocr Disord. 2024 Nov 26;24(1):255. doi:
            10.1186/s12902-024-01786-z.. PMID: 39587564]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Wang,X., Li,Z., Qu,M., Xiong,C. and Li,H.
  TITLE     A homozygous PIWIL2 frameshift variant affects the formation and
            maintenance of human-induced pluripotent stem cell-derived
            spermatogonial stem cells and causes Sertoli cell-only syndrome
  JOURNAL   Stem Cell Res Ther 13 (1), 480 (2022)
   PUBMED   36153567
  REMARK    GeneRIF: A homozygous PIWIL2 frameshift variant affects the
            formation and maintenance of human-induced pluripotent stem
            cell-derived spermatogonial stem cells and causes Sertoli cell-only
            syndrome.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5114)
  AUTHORS   Tan,X., Mi,T., Zhang,Z., Jin,L., Wang,Z., Wu,X., Wang,J., Li,M.,
            Zhanghuang,C. and He,D.
  TITLE     Multiple transcriptome analysis of Piwil2-induced cancer stem
            cells, including piRNAs, mRNAs and miRNAs reveals the mechanism of
            tumorigenesis and development
  JOURNAL   Mol Biol Rep 49 (7), 6885-6898 (2022)
   PUBMED   35182325
  REMARK    GeneRIF: Multiple transcriptome analysis of Piwil2-induced cancer
            stem cells, including piRNAs, mRNAs and miRNAs reveals the
            mechanism of tumorigenesis and development.
REFERENCE   4  (bases 1 to 5114)
  AUTHORS   Zhou,S., Yang,S., Li,F., Hou,J. and Chang,H.
  TITLE     P-element Induced WImpy protein-like RNA-mediated gene silencing 2
            regulates tumor cell progression, apoptosis, and metastasis in oral
            squamous cell carcinoma
  JOURNAL   J Int Med Res 49 (11), 3000605211053158 (2021)
   PUBMED   34738477
  REMARK    GeneRIF: P-element Induced WImpy protein-like RNA-mediated gene
            silencing 2 regulates tumor cell progression, apoptosis, and
            metastasis in oral squamous cell carcinoma.
REFERENCE   5  (bases 1 to 5114)
  AUTHORS   Giebler,M., Greither,T., Handke,D., Seliger,G. and Behre,H.M.
  TITLE     Lower Spermatozoal PIWI-LIKE 1 and 2 Transcript Levels Are
            Significantly Associated with Higher Fertilization Rates in IVF
  JOURNAL   Int J Mol Sci 22 (21), 11320 (2021)
   PUBMED   34768750
  REMARK    GeneRIF: Lower Spermatozoal PIWI-LIKE 1 and 2 Transcript Levels Are
            Significantly Associated with Higher Fertilization Rates in IVF.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5114)
  AUTHORS   Nikpour,P., Forouzandeh-Moghaddam,M., Ziaee,S.A., Dokun,O.Y.,
            Schulz,W.A. and Mowla,S.J.
  TITLE     Absence of PIWIL2 (HILI) expression in human bladder cancer cell
            lines and tissues
  JOURNAL   Cancer Epidemiol 33 (3-4), 271-275 (2009)
   PUBMED   19683485
  REMARK    GeneRIF: our study indicates that PIWIL2 does not play a role in
            carcinogenesis of human bladder carcinoma
REFERENCE   7  (bases 1 to 5114)
  AUTHORS   Lee,J.H., Schutte,D., Wulf,G., Fuzesi,L., Radzun,H.J., Schweyer,S.,
            Engel,W. and Nayernia,K.
  TITLE     Stem-cell protein Piwil2 is widely expressed in tumors and inhibits
            apoptosis through activation of Stat3/Bcl-XL pathway
  JOURNAL   Hum Mol Genet 15 (2), 201-211 (2006)
   PUBMED   16377660
  REMARK    GeneRIF: Stem-cell protein Piwil2 is widely expressed in tumors and
            inhibits apoptosis through activation of Stat3/Bcl-XL pathway.
REFERENCE   8  (bases 1 to 5114)
  AUTHORS   Kuramochi-Miyagawa,S., Kimura,T., Ijiri,T.W., Isobe,T., Asada,N.,
            Fujita,Y., Ikawa,M., Iwai,N., Okabe,M., Deng,W., Lin,H., Matsuda,Y.
            and Nakano,T.
  TITLE     Mili, a mammalian member of piwi family gene, is essential for
            spermatogenesis
  JOURNAL   Development 131 (4), 839-849 (2004)
   PUBMED   14736746
REFERENCE   9  (bases 1 to 5114)
  AUTHORS   Sasaki,T., Shiohama,A., Minoshima,S. and Shimizu,N.
  TITLE     Identification of eight members of the Argonaute family in the
            human genome
  JOURNAL   Genomics 82 (3), 323-330 (2003)
   PUBMED   12906857
REFERENCE   10 (bases 1 to 5114)
  AUTHORS   Wang,P.J., McCarrey,J.R., Yang,F. and Page,D.C.
  TITLE     An abundance of X-linked genes expressed in spermatogonia
  JOURNAL   Nat Genet 27 (4), 422-426 (2001)
   PUBMED   11279525
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC105206.8 and AC105910.10.
            
            On Nov 23, 2018 this sequence version replaced NM_018068.4.
            
            Summary: PIWIL2 belongs to the Argonaute family of proteins, which
            function in development and maintenance of germline stem cells
            (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar
            2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK291038.1, AK056418.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2145245
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356766.11/ ENSP00000349208.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-83                AC105206.8         12450-12532         c
            84-327              AC105206.8         8264-8507           c
            328-415             AC105206.8         6641-6728           c
            416-554             AC105206.8         6333-6471           c
            555-761             AC105206.8         4608-4814           c
            762-872             AC105206.8         3576-3686           c
            873-990             AC105206.8         203-320             c
            991-1115            AC105910.10        118660-118784       c
            1116-1196           AC105910.10        117399-117479       c
            1197-1310           AC105910.10        116980-117093       c
            1311-1499           AC105910.10        103117-103305       c
            1500-1584           AC105910.10        102458-102542       c
            1585-1674           AC105910.10        101310-101399       c
            1675-1815           AC105910.10        99253-99393         c
            1816-1929           AC105910.10        97252-97365         c
            1930-2118           AC105910.10        96026-96214         c
            2119-2220           AC105910.10        92897-92998         c
            2221-2337           AC105910.10        92181-92297         c
            2338-2426           AC105910.10        90993-91081         c
            2427-2532           AC105910.10        89051-89156         c
            2533-2786           AC105910.10        54114-54367         c
            2787-2894           AC105910.10        52948-53055         c
            2895-5114           AC105910.10        49758-51977         c
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p21.3"
     gene            1..5114
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /note="piwi like RNA-mediated gene silencing 2"
                     /db_xref="GeneID:55124"
                     /db_xref="HGNC:HGNC:17644"
                     /db_xref="MIM:610312"
     exon            1..83
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            84..327
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..3051
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /note="isoform 1 is encoded by transcript variant 2;
                     cancer/testis antigen 80; piwi-like protein 2; piwil2-like
                     protein; piwi-like 2; testicular tissue protein Li 141"
                     /codon_start=1
                     /product="piwi-like protein 2 isoform 1"
                     /protein_id="NP_060538.2"
                     /db_xref="CCDS:CCDS6029.1"
                     /db_xref="GeneID:55124"
                     /db_xref="HGNC:HGNC:17644"
                     /db_xref="MIM:610312"
                     /translation="MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAP
                     AGRGHVFGKPEEPSTQRGPAQRESVGLVSMFRGLGIETVSKTPLKREMLPSGRGILGR
                     GLSANLVRKDREELSPTFWDPKVLAAGDSKMAETSVGWSRTLGRGSSDASLLPLGRAA
                     GGISREVDKPPCTFSTPSRGPPQLSSPPALPQSPLHSPDRPLVLTVEHKEKELIVKQG
                     SKGTPQSLGLNLVKIQCHNEAVYQYHVTFSPNVECKSMRFGMLKDHQAVTGNVTAFDG
                     SILYLPVKLQQVLELKSQRKTDSAEISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLL
                     DMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDCVLD
                     VMHAIYQQNKEHFQDECTKLLVGNIVITRYNNRTYRIDDVDWNKTPKDSFTMSDGKEI
                     TFLEYYSKNYGITVKEEDQPLLIHRPSERQDNHGMLLKGEILLLPELSFMTGIPEKMK
                     KDFRAMKDLAQQINLSPKQHHSALECLLQRIAKNEAATNELMRWGLRLQKDVHKIEGR
                     VLPMERINLKNTSFITSQELNWVKEVTRDPSILTIPMHFWALFYPKRAMDQARELVNM
                     LEKIAGPIGMRMSPPAWVELKDDRIETYVRTIQSTLGAEGKIQMVVCIIMGPRDDLYG
                     AIKKLCCVQSPVPSQVVNVRTIGQPTRLRSVAQKILLQINCKLGGELWGVDIPLKQLM
                     VIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKK
                     FYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFENYQPKMVVFVVQKKI
                     STNLYLAAPQNFVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYVCVLNTAN
                     LSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHHEPAIQLCENLFF
                     L"
     exon            328..415
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            416..554
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            555..761
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            762..872
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            873..990
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1115
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1196
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1310
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1499
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1584
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1674
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1815
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1929
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1930..2118
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2220
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2337
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2338..2426
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..2532
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2786
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2787..2894
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2895..5114
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5093..5098
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /note="hexamer: AATAAA"
     polyA_site      5114
                     /gene="PIWIL2"
                     /gene_synonym="CT80; HILI; mili; PIWIL1L"
                     /note="major polyA site"
ORIGIN      
        1 gtgggccccg gggcgtgggt tgagctcggt cttcccctga ggccgcgcgg agctgggcga
       61 ctggggcgag gactcgcgca caggtaatta accagaacag gatcgacacg tgttctctac
      121 agcccgtcca tggatccttt ccgaccatcg ttcaggggcc agtctcctat ccacccatcc
      181 cagtgccagg ctgtacggat gccaggctgt tggccacaag cttctaaacc tttggaccca
      241 gctctgggca ggggagcacc tgcaggcaga ggccatgtat ttggaaagcc agaggaacca
      301 agcacacaga gggggccagc acaaagggag tctgtgggtt tggtctccat gttccgaggc
      361 ctgggcattg aaacagtttc taagacccct ctgaaacggg aaatgcttcc atcaggtaga
      421 ggcattttag gtcgaggctt gtctgctaat ctggtacgca aggacaggga ggaactctct
      481 cccacttttt gggatccaaa agtgttggcg gctggggaca gcaagatggc agagacctcc
      541 gttggttgga gtaggacgct tggaagaggg agttcagatg cgtctttatt accactggga
      601 agagcagcag gtggtatcag cagagaagtg gacaagcctc cctgtacctt cagcacaccg
      661 tcccggggtc ccccgcagct gtcatcacca ccagctctgc cccagtctcc cctgcactct
      721 ccagatcgcc ctctggtcct gactgtggaa cacaaggaaa aagagcttat tgtgaagcaa
      781 ggatcaaaag gaacacctca gtctttggga ctgaacctcg tcaaaataca gtgtcataat
      841 gaagcagttt atcaatatca tgtgactttc agccccaatg tggagtgcaa aagcatgagg
      901 ttcggcatgt tgaaggacca tcaagctgtc accggcaacg tcactgcgtt tgatggatct
      961 attctctatc tgcctgttaa gcttcaacaa gttcttgagt taaaaagtca aaggaaaaca
     1021 gacagtgctg aaatcagcat taagattcag atgacaaaga tcctggagcc ctgctctgac
     1081 ctgtgcattc ccttctacaa tgttgttttc cgtcgggtaa tgaaactttt agatatgaag
     1141 cttgtgggga gaaactttta tgaccctaca agtgctatgg tactacagca acacagattg
     1201 cagatctggc caggctatgc agctagcatc cgaaggacag atggagggct cttcctgcta
     1261 gctgatgtct cccataaggt cattcggaat gactgtgtgc tggatgtcat gcatgccatt
     1321 tatcagcaga ataaagaaca cttccaggat gagtgtacta agcttctggt tggcaatatt
     1381 gttatcaccc gatataacaa tcgtacctat cgtattgatg atgtggattg gaataagact
     1441 ccaaaggata gcttcacgat gtctgatggg aaagagatca cattcttgga atactacagc
     1501 aaaaattatg ggatcacagt taaggaagag gaccagccat tgctgattca caggcccagt
     1561 gagagacagg ataatcatgg gatgctgcta aaaggggaaa tcctgctgct gcctgagctt
     1621 tcttttatga ccggaatccc agagaagatg aagaaggact tcagagccat gaaggatttg
     1681 gctcagcaaa tcaatctgag ccccaagcaa caccatagtg ctttggaatg cttgctgcaa
     1741 agaattgcaa agaacgaggc agccaccaat gaactgatgc gttgggggct ccgtctgcaa
     1801 aaggatgtac ataagattga aggacgtgtt ctgccaatgg aaagaattaa cttaaaaaat
     1861 acttcgttta tcacatctca ggaactaaac tgggttaagg aagtaaccag agacccttcc
     1921 atcttgacta tccccatgca tttctgggca cttttttacc caaagagagc aatggaccag
     1981 gctcgagaac tggtcaacat gttggagaag atagccggcc ccattggcat gcgtatgagc
     2041 ccaccggcct gggttgaact aaaggatgac cgaatagaga cttatgtcag aaccattcaa
     2101 tccacgttag gagctgaggg gaagatacag atggttgttt gcatcatcat gggcccacgt
     2161 gatgatctct atggggccat caagaagctg tgctgtgtgc agtccccagt gccctcccag
     2221 gttgtcaatg ttcgaaccat tggtcagccc accaggcttc ggagtgtggc ccagaagatt
     2281 ttacttcaga ttaactgtaa attgggtggt gagctctggg gagtggatat tcctctgaaa
     2341 cagttaatgg tgatcgggat ggatgtttac catgacccca gtagaggcat gcgctccgtg
     2401 gttggcttcg tggcaagcat caatctcacc ctcacaaaat ggtattcccg ggtggtgttc
     2461 cagatgccgc atcaggagat tgtggacagc ctgaagctat gcctcgtggg ctccttaaaa
     2521 aagttttatg aggtgaacca ctgtctacca gagaagattg tggtgtaccg tgatggagtg
     2581 tctgatggcc aactgaagac agttgccaac tatgagattc ctcaactaca gaagtgtttt
     2641 gaagcttttg agaattatca gcccaagatg gtggtgtttg tagttcagaa gaaaatcagt
     2701 actaatctat atctggctgc tcctcagaac tttgtaactc ccactcctgg aactgtggta
     2761 gatcatacaa taacaagctg tgagtgggtg gatttctatc ttcttgccca tcatgtacgg
     2821 cagggctgtg gcattcctac gcattatgtc tgtgttctca acaccgcaaa cctgagccct
     2881 gatcatatgc agaggctgac tttcaaactg tgccacatgt actggaattg gcctggcacc
     2941 atcagagttc cagctccttg caagtatgcc cacaagctag ctttcctgtc aggacacatc
     3001 ttgcatcatg aaccagccat ccagctgtgc gagaacctgt tcttcctgtg actgcacagc
     3061 ttggagatgg gctggtgaga agaaaggcgg cctcagaact cagctgtgac tcttgcagaa
     3121 tcaacagaga ctgaagtggg cttttgtgtt ataattttcc ctttctccaa ccctgtagaa
     3181 taagatttct ttcttgtctt ttaaacctaa tatcaccaag aagcaagttt ctgagtaaca
     3241 gctgaaaatg gccttgttgc ctgtgtagag caagttacgg tggtactgcc actctgcagg
     3301 tggagcgggt gactctgggg gaccattaag acctccagac cgggtgcggt ggttcacacc
     3361 tgtaatccaa gcactttggg aggccgaggc gggtggatca tgaggtcagg agatcaagac
     3421 catcctggcc aacatggtga aaccccgtct ctactaaaat acaaaaaaat tagccgggtg
     3481 tggcggtgca cgcctgtagt cccagctact caggaggcta aggcaggaga atcgcttgaa
     3541 cccgggaggt ggaggttgca gtgagccgag atcacgccac tgcactccag cctgttgaca
     3601 aagcaagact ctgtctcaaa aaaaaaaaaa aaaagccaac atgagctgca gaaaccagca
     3661 gaagcagcca ttatacttct aagctgtgcc aactaagtgg ttcccatttc tggagccaga
     3721 gatgataatt aagtcattgc aagttgcctg aagagaatta ctcaggcaac tgggaaaggc
     3781 agtggaaggt ctgtgaagca aagttttcaa gtagatattg ctattactgg caggtgtgca
     3841 aggcgtgtag taaaatcagt ttgtggcata ggtcggccct tgctgactaa atcctctctg
     3901 gggattgaat tgatgacgac tgtgacttga gttggattcg aaaatggctt cccttggcct
     3961 cttgaaagag tgcagatgaa gccttttccg tggatttcag taactttcca gcataccatg
     4021 acgatttctt ccccaaatat acacatgctc ttttgctctt tgtgcctgat cttcagtaat
     4081 ttttagtttt ttaacaggca gaaaaaatct agcagctagg aaagttcaag ttggggtggg
     4141 agtttttagt gaacttttct cccaggtgtg tggcctttat ttctatttat atatgtttgt
     4201 aaatggaatt accccctttc tcttgcaaaa taaccgcttc ctggctgttg gctcactttg
     4261 ctggaatttt tctgaatcca ggttaagatt tccagaagtc agctttggaa aacgtgacac
     4321 tcctaccaaa gtccagatgg ttagaaagtt atcacatacc atgggtttca gtgtttttgg
     4381 aaacccgttt ttttctggtt ttttttcccc cctctccatt ttagaatctt ctcccagtcc
     4441 cttcgggcct agttctgtct catcttgctt tattgggttt taaaaaccgg tcttatttaa
     4501 gcttttctaa tttgtattta tgtacttatt tattttttgg tatgatggca gggaggggac
     4561 aaagcagaaa tacccctttt atatgacaat gtgtgagaaa agaaacttta gcttaaaact
     4621 ccgtaaaatg tgctcacttc agcagcacat atactaaaat taaaatgata tagagaagat
     4681 tagcatatag aaaaaaagaa aaaagatttc cttaaaacaa gaaaaccaag aatgtttttg
     4741 gtttgttgcg gtgcccagcc gaggttgagg agggatgggt attgttggtg cactgctggt
     4801 ttcatttgga gtgtgggaca ttagggtgag acaaggacct ggaatttaaa aaccacagaa
     4861 tcaaacactg tgaaccactg gtctctggct aagcagatca gttttctgat tttccttgga
     4921 agcgttttga atttttctgt tgaagttgga tttcctaata ctttttttct cttagataat
     4981 cagaacaaat ggttgaatac attataatac aaaaatttct tcacttttta ttcttggttt
     5041 tcttgcctcg tttttcggtt ttaatgccaa atgattgggt catggggtgg gaaataaaaa
     5101 caactttgta taaa
//
