LOCUS       NM_001101387            3457 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens phosphoinositide interacting regulator of transient
            receptor potential channels (PIRT), mRNA.
ACCESSION   NM_001101387 XM_927351 XM_938565
VERSION     NM_001101387.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Sisco,N.J., Helsell,C.V.M. and Van Horn,W.D.
  TITLE     Competitive Interactions between PIRT, the Cold Sensing Ion Channel
            TRPM8, and PIP2 Suggest a Mechanism for Regulation
  JOURNAL   Sci Rep 9 (1), 14128 (2019)
   PUBMED   31575973
  REMARK    GeneRIF: Competitive Interactions between PIRT, the Cold Sensing
            Ion Channel TRPM8, and PIP2 Suggest a Mechanism for Regulation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Hilton,J.K., Salehpour,T., Sisco,N.J., Rath,P. and Van Horn,W.D.
  TITLE     Phosphoinositide-interacting regulator of TRP (PIRT) has opposing
            effects on human and mouse TRPM8 ion channels
  JOURNAL   J Biol Chem 293 (24), 9423-9434 (2018)
   PUBMED   29724821
  REMARK    GeneRIF: These results have uncovered species-specific TRPM8
            modulation by PIRT. They provide evidence for a direct interaction
            between PIRT and the TRPM8 S1-S4 domain with a 1:1 binding
            stoichiometry, suggesting that a functional tetrameric TRPM8
            channel has four PIRT-binding sites.
REFERENCE   3  (bases 1 to 3457)
  AUTHORS   Kim,A.Y., Tang,Z., Liu,Q., Patel,K.N., Maag,D., Geng,Y. and Dong,X.
  TITLE     Pirt, a phosphoinositide-binding protein, functions as a regulatory
            subunit of TRPV1
  JOURNAL   Cell 133 (3), 475-485 (2008)
   PUBMED   18455988
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC332889.1 and AC015908.10.
            
            On Nov 22, 2018 this sequence version replaced NM_001101387.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK299808.1, SRR1803617.142240.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000580256.3/ ENSP00000462046.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-546               DC332889.1         15-560
            547-3452            AC015908.10        72663-75568
            3453-3457           AC015908.10        75569-75573
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p12"
     gene            1..3457
                     /gene="PIRT"
                     /note="phosphoinositide interacting regulator of transient
                     receptor potential channels"
                     /db_xref="GeneID:644139"
                     /db_xref="HGNC:HGNC:37239"
                     /db_xref="MIM:612068"
     exon            1..143
                     /gene="PIRT"
                     /inference="alignment:Splign:2.1.0"
     exon            144..3457
                     /gene="PIRT"
                     /inference="alignment:Splign:2.1.0"
     CDS             282..695
                     /gene="PIRT"
                     /note="phosphoinositide-interacting regulator of TRPV1"
                     /codon_start=1
                     /product="phosphoinositide-interacting protein"
                     /protein_id="NP_001094857.1"
                     /db_xref="CCDS:CCDS45614.1"
                     /db_xref="GeneID:644139"
                     /db_xref="HGNC:HGNC:37239"
                     /db_xref="MIM:612068"
                     /translation="MTMETLPKVLEVDEKSPEAKDLLPSQTASSLCISSRSESVWTTT
                     PRSNWEIYRKPIVIMSVGGAILLFGVVITCLAYTLKLSDKSLSILKMVGPGFLSLGLM
                     MLVCGLVWVPIIKKKQKHRQKSNFLRSLKSFFLTR"
     polyA_site      3457
                     /gene="PIRT"
                     /note="major polyA site"
ORIGIN      
        1 acaacacact gacttctgga cgtggtcagg gtggctttct ctggctggca tggacttgag
       61 cagaaactca ttgccggctt cctccttgca cgagcaccaa gtgattccca ggtcccccat
      121 cctcccctgg gacatcgagg acacacaaaa accttgttca ccctccactg agagcggggc
      181 tagagatgga tgctgagtac aggaagggtg tgtgaggatt cctttggctc cactcttgtt
      241 tccaactatt gctggtccta ggcgcccagt cctgagcaac catgacgatg gagactctcc
      301 ccaaggttct agaggtcgat gagaagtctc cagaagccaa ggacctgctg cccagccaga
      361 ccgccagctc cctgtgcatc agctccagga gcgagtctgt ctggaccacc acccccagga
      421 gtaactggga aatctaccgc aagcccatcg ttatcatgtc agtgggcggt gccatcctgc
      481 ttttcggcgt ggtcatcacc tgcttggcct acaccttgaa gctgagtgac aagagtctct
      541 ccatcctcaa aatggtaggg cctggcttcc tgtccctggg actcatgatg ctggtgtgcg
      601 ggctggtgtg ggtgcccatc atcaaaaaga aacagaagca cagacagaag tcgaatttct
      661 tacgcagcct caagtccttc ttcctgactc gctgatggtg gtttccgcat ctccctgcca
      721 ccttgactgg gagaagatct gatgaccaac atccgacatc cctgtctcct ctgtggggtg
      781 ccataaaact gatccaggaa aatgctcttc ctgaccctgt ggggaagata gagcttgggg
      841 ctccaccctc catgcagccg ggaatgttgc cagatgtatc cacttgatct ctattctgac
      901 tgatgcttcc ttggatcccg gctgtggcct tccctttggt cactctaacc ccctgcccct
      961 gcatccaggc atcattcatc caactctctc tgccaagtgc aatgcatgct gggaaattct
     1021 tcgaaggtgg gctatgccca agggagaacc tcatgtttac atatctgagt gccagagctg
     1081 gattcttcat ttgcatactt ctggaaccaa aactgaccca cggaggtcct ttacagagga
     1141 gggagggatg gctgagctct gacccattgg gtttgacagc cctagctccc ttagcagact
     1201 ttcaagatca caagtatcaa acattccaaa gactgaacaa tgaagggagg cctgggaaaa
     1261 atactctcca agatggcacg tgcccctgga catgacaaca tgactctagg ggttagtcag
     1321 ctgaagaagt ttagttccta attatataat atttacatca gaggcagtgg ggtaggctag
     1381 gggatgtcac tgtttccgct tgaccttatc tttctcattg accactgtct tctctgaact
     1441 tgatcattag ccaaggacaa aatgcatgtt gccctaattt ctttcctacc cctgcctcta
     1501 tctctgcatc tagaacagct aaatggaaag atacagaggg atcctgtaag gttgttttcc
     1561 ctttcacgtc catcaagatc aaaattgaga gaatcaagtt ccatatctga atgatctgta
     1621 ggtttctaga gatggaattc acgtcagaaa attccaactt ctgttatccc tgcccccacc
     1681 caccaaaaaa gccaacattt attaagtgtc gcattctggg ataagccctg gctgggggca
     1741 agacacggtc cctgatgtct tcttgcttcc cgaggtgaga gaggcagcca agccaactgg
     1801 cgaaatgtgg gtgggtgtgg gaacacaggc ggtgtctacc ctagacttga tagaggacgg
     1861 agaaattcct ggaggccatg tcctccaacc caaaaacaaa aggaaagata agagttacca
     1921 aagaggggaa agaatggcat tggaagcagc aggagctgct atgcgtgtgc aggagtagct
     1981 tggggaagga gaagcatgat ggaattagga aactgcacgg ccatttgtct gacctcccag
     2041 atgtttttac ttcatccctc tctttcccgg cttcccacct cttcaccaag tgcctagcgc
     2101 agcttctcct ggaggcacat tgagggcctc ctggtgtcta tggtagagac gggaagtggg
     2161 tttccagagg gcctggcaag caaagagtcc ctgagaagtg cttctgtaac tccctgtggc
     2221 aaccaagagg cccgtaggtg aaagaacaga agcagtacct gtgagggagc cagccacgct
     2281 gccctaggcc aaagacccct ggagagcatt tggtaatgag gtgtgagagg agagcatcct
     2341 gcaccagaat cacccttgtc ttgagctaat tctcttcact cctcagcctt gtggccttcc
     2401 cagtcaccat ggccctccag atatccccat cctgcatggc agttccatat gcagagccag
     2461 aatccatcct tgttccttct gcattgtgag aaaatccatg gaggaaagca cttgtgtcat
     2521 ggggaccacc cctacctgac ccacccacgg agtcagagtt tggggaactc atggccgttg
     2581 aggctcaccc aggatggcca tagccttcat ggagacccat gccagtgtgg ccatgtctct
     2641 agctcacctc tagccctgca cacaggtggt caggaccaat cttgggagtg ttgcaagtgc
     2701 tatgtcgtta attgtagttt tagtgcctca gtgacaaacc ttgcagatca tttcccttct
     2761 tctgagatga atgcagattt gatggcccta taatattact cttgcattcc tcgatgcatt
     2821 cttaaatggg agcaaatggg atgggcccca gatcactcac tggaaaccca cactccaggt
     2881 gcctcatttc ctctgcccag gagattgtag cctgggcaca gctatatgag gagggcccat
     2941 ttggatactg ctatcatgga tgtcttagtg gaaggaagtt tagggataac cacaactcta
     3001 gaagtccagg ttctggactc actttgcaga ctctagcagt acctgcctcc attccctgcc
     3061 cactcaagct agtttctcag caaagagaat atgaggaaat atccccaaac atcgtcatcc
     3121 ccactgggat cctccacatg agtgtgggct ggaaaggttc cagggtcaca ctgaggcagg
     3181 acattttttg ccaatgcctg ttaccccatg gaaggctgag gtacggatgc acctgatctg
     3241 gaaatgcagc ccaggtcaga ctccacattt ctgcaacaaa gcatcacctg caatgactct
     3301 tttcacgtca gtgggtcaaa ggacagaacc agcttccgtg ttttaggctc actagattgt
     3361 ctcatgctcc ccatggctat tctgtttggg agaatttgat ctaacccgaa gcctaaagga
     3421 atcaatgtaa tgttgaattg tattctatgt aacactg
//
