LOCUS       NM_001018109            1274 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens pirin (PIR), transcript variant 2, mRNA.
ACCESSION   NM_001018109
VERSION     NM_001018109.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Xie,J., Xing,S., Shen,B.Y., Chen,H.T., Sun,B., Wang,Z.T., Wang,J.W.
            and Lu,X.X.
  TITLE     PIWIL1 interacting RNA piR-017061 inhibits pancreatic cancer growth
            via regulating EFNA5
  JOURNAL   Hum Cell 34 (2), 550-563 (2021)
   PUBMED   33389678
  REMARK    GeneRIF: PIWIL1 interacting RNA piR-017061 inhibits pancreatic
            cancer growth via regulating EFNA5.
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Hu,N., Bai,L., Dai,E., Han,L., Kang,R., Li,H. and Tang,D.
  TITLE     Pirin is a nuclear redox-sensitive modulator of autophagy-dependent
            ferroptosis
  JOURNAL   Biochem Biophys Res Commun 536, 100-106 (2021)
   PUBMED   33373853
  REMARK    GeneRIF: Pirin is a nuclear redox-sensitive modulator of
            autophagy-dependent ferroptosis.
REFERENCE   3  (bases 1 to 1274)
  AUTHORS   Suleman,M., Chen,A., Ma,H., Wen,S., Zhao,W., Lin,D., Wu,G. and
            Li,Q.
  TITLE     PIR promotes tumorigenesis of breast cancer by upregulating cell
            cycle activator E2F1
  JOURNAL   Cell Cycle 18 (21), 2914-2927 (2019)
   PUBMED   31500513
  REMARK    GeneRIF: PIR promotes tumorigenesis of breast cancer by
            upregulating cell cycle activator E2F1.
REFERENCE   4  (bases 1 to 1274)
  AUTHORS   Arai,T., Kojima,S., Yamada,Y., Sugawara,S., Kato,M., Yamazaki,K.,
            Naya,Y., Ichikawa,T. and Seki,N.
  TITLE     Pirin: a potential novel therapeutic target for
            castration-resistant prostate cancer regulated by miR-455-5p
  JOURNAL   Mol Oncol 13 (2), 322-337 (2019)
   PUBMED   30444038
  REMARK    GeneRIF: miR-455-5p/PIR axis contributed to cancer cell
            aggressiveness.
REFERENCE   5  (bases 1 to 1274)
  AUTHORS   Adeniran,C. and Hamelberg,D.
  TITLE     Redox-Specific Allosteric Modulation of the Conformational Dynamics
            of kappaB DNA by Pirin in the NF-kappaB Supramolecular Complex
  JOURNAL   Biochemistry 56 (37), 5002-5010 (2017)
   PUBMED   28825294
  REMARK    GeneRIF: Data suggest that only the Fe(III) form of pirin [not the
            Fe(II) form] enhances affinity of binding between p65 and DNA in
            the pirin-p65-DNA supramolecular complex. (pirin = quercetin
            2,3-dioxygenase; p65 = RELA proto-oncogene NF-kappaB subunit)
REFERENCE   6  (bases 1 to 1274)
  AUTHORS   Colland,F., Jacq,X., Trouplin,V., Mougin,C., Groizeleau,C.,
            Hamburger,A., Meil,A., Wojcik,J., Legrain,P. and Gauthier,J.M.
  TITLE     Functional proteomics mapping of a human signaling pathway
  JOURNAL   Genome Res 14 (7), 1324-1332 (2004)
   PUBMED   15231748
REFERENCE   7  (bases 1 to 1274)
  AUTHORS   Pang,H., Bartlam,M., Zeng,Q., Miyatake,H., Hisano,T., Miki,K.,
            Wong,L.L., Gao,G.F. and Rao,Z.
  TITLE     Crystal structure of human pirin: an iron-binding nuclear protein
            and transcription cofactor
  JOURNAL   J Biol Chem 279 (2), 1491-1498 (2004)
   PUBMED   14573596
  REMARK    GeneRIF: x-ray crystallography of of human pirin shows that it is
            an iron-binding nuclear protein and transcription cofactor
REFERENCE   8  (bases 1 to 1274)
  AUTHORS   Zeng,Q., Li,X., Bartlam,M., Wang,G., Pang,H. and Rao,Z.
  TITLE     Purification, crystallization and preliminary X-ray analysis of
            human pirin
  JOURNAL   Acta Crystallogr D Biol Crystallogr 59 (Pt 8), 1496-1498 (2003)
   PUBMED   12876364
REFERENCE   9  (bases 1 to 1274)
  AUTHORS   Dechend,R., Hirano,F., Lehmann,K., Heissmeyer,V., Ansieau,S.,
            Wulczyn,F.G., Scheidereit,C. and Leutz,A.
  TITLE     The Bcl-3 oncoprotein acts as a bridging factor between
            NF-kappaB/Rel and nuclear co-regulators
  JOURNAL   Oncogene 18 (22), 3316-3323 (1999)
   PUBMED   10362352
REFERENCE   10 (bases 1 to 1274)
  AUTHORS   Wendler,W.M., Kremmer,E., Forster,R. and Winnacker,E.L.
  TITLE     Identification of pirin, a novel highly conserved nuclear protein
  JOURNAL   J Biol Chem 272 (13), 8482-8489 (1997)
   PUBMED   9079676
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA686501.1, AC097625.11 and
            BC002517.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001018109.2.
            
            Summary: This gene encodes a member of the cupin superfamily. The
            encoded protein is an Fe(II)-containing nuclear protein expressed
            in all tissues of the body and concentrated within dot-like
            subnuclear structures. Interactions with nuclear factor I/CCAAT box
            transcription factor as well as B cell lymphoma 3-encoded
            oncoprotein suggest the encoded protein may act as a
            transcriptional cofactor and be involved in the regulation of DNA
            transcription and replication. Alternatively spliced transcript
            variants have been described. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate, in-frame
            splice site in the 5' UTR, compared to variant 1. Variants 1 and 2
            encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y07867.1, SRR1803614.105885.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380420.10/ ENSP00000369785.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                DA686501.1         256-299
            45-97               AC097625.11        72623-72675         c
            98-1274             BC002517.2         1-1177
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.2"
     gene            1..1274
                     /gene="PIR"
                     /note="pirin"
                     /db_xref="GeneID:8544"
                     /db_xref="HGNC:HGNC:30048"
                     /db_xref="MIM:300931"
     exon            1..144
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            145..292
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     CDS             197..1069
                     /gene="PIR"
                     /EC_number="1.13.11.24"
                     /note="probable quercetinase; probable quercetin
                     2,3-dioxygenase PIR; pirin (iron-binding nuclear protein)"
                     /codon_start=1
                     /product="pirin"
                     /protein_id="NP_001018119.1"
                     /db_xref="CCDS:CCDS14167.1"
                     /db_xref="GeneID:8544"
                     /db_xref="HGNC:HGNC:30048"
                     /db_xref="MIM:300931"
                     /translation="MGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDE
                     FKGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHA
                     EMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIK
                     SKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTA
                     VLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKN
                     GFERAKTWKSKIGN"
     exon            293..385
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            386..469
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            470..676
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            677..761
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            762..806
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            807..889
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            890..956
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1274
                     /gene="PIR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1246..1251
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIR"
                     /note="hexamer: AATAAA"
     polyA_site      1274
                     /gene="PIR"
                     /note="major polyA site"
ORIGIN      
        1 ctcctctagg ccgccggccg cgaagcgctg agtcacggtg aggctactgg acccacactc
       61 tcttaacctg ccctccctgc actcgctccc ggcggctctt cgcgtcaccc ccgccgctaa
      121 ggctccaggt gccgctaccg cagcccctcc atcctctaca gctcagcatc agaacactct
      181 ctttttagac tccgatatgg ggtcctccaa gaaagttact ctctcagtgc tcagccggga
      241 gcagtcggaa ggggttggag cgagggtccg gagaagcatt ggcagacccg agttaaaaaa
      301 tctggatccg tttttactgt ttgatgaatt taaaggaggt agaccaggag gatttcctga
      361 tcatccacat cgaggttttg aaacagtatc ctacctcctg gaagggggca gcatggccca
      421 tgaagacttc tgtggacaca ctggtaaaat gaacccagga gatttgcagt ggatgactgc
      481 gggccggggc attctgcacg ctgagatgcc ttgctcagag gagccagccc atggcctaca
      541 actgtgggtt aatttgagga gctcagagaa gatggtggag cctcagtacc aggaactgaa
      601 aagtgaagaa atccctaaac ccagtaagga tggtgtgaca gttgctgtca tttctggaga
      661 agccctggga ataaagtcca aggtttacac tcgcacacca accttatatt tggacttcaa
      721 attggaccca ggagccaaac attcccaacc tatccctaaa gggtggacaa gcttcattta
      781 cacgatatct ggagatgtgt atattgggcc cgatgatgca caacaaaaaa tagaacctca
      841 tcacacagca gtgcttggag aaggtgacag tgtccaggtg gagaacaagg atcccaagag
      901 aagccacttt gtcttaattg ctggggagcc attaagagaa ccagttatcc aacatggtcc
      961 atttgtgatg aacaccaatg aagagatttc tcaagctatt cttgatttca gaaacgcaaa
     1021 aaatgggttt gaaagggcca aaacctggaa atcaaagatt gggaactagt ggaaagcgga
     1081 agagcaggtc ttgatgtgtc ctagaatttt gccatttctg agattgagcc attgaaggca
     1141 ttccatttct aaagcttatt tagccggtgc ttctaaagaa ttccacacta acgtgataac
     1201 atggtttttg taacaataaa tgtaggatat ttcctggcac atgcaaataa acctaatcat
     1261 tgtttcttta aaaa
//
