LOCUS       NM_002648               2703 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens Pim-1 proto-oncogene, serine/threonine kinase (PIM1),
            transcript variant 1, mRNA.
ACCESSION   NM_002648
VERSION     NM_002648.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Noura,M., Tomita,S., Yasuda,T., Tsuzuki,S., Kiyoi,H. and
            Hayakawa,F.
  TITLE     NUP98-BPTF promotes oncogenic transformation through PIM1
            upregulation
  JOURNAL   Cancer Med 13 (13), e7445 (2024)
   PUBMED   38940430
  REMARK    GeneRIF: NUP98-BPTF promotes oncogenic transformation through PIM1
            upregulation.
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Gao,X., Chu,L.J. and Yan,Z.H.
  TITLE     [Effects of PIM1 Gene on Proliferation, Apoptosis and JAK2/STAT3
            Signaling Pathway of Acute Myeloid Leukemia U937 Cells]
  JOURNAL   Zhongguo Shi Yan Xue Ye Xue Za Zhi 32 (3), 663-669 (2024)
   PUBMED   38926951
  REMARK    GeneRIF: [Effects of PIM1 Gene on Proliferation, Apoptosis and
            JAK2/STAT3 Signaling Pathway of Acute Myeloid Leukemia U937 Cells].
REFERENCE   3  (bases 1 to 2703)
  AUTHORS   Hochban,P.M.M., Heyder,L., Heine,A. and Diederich,W.E.
  TITLE     What doesn't fit is made to fit: Pim-1 kinase adapts to the
            configuration of stilbene-based inhibitors
  JOURNAL   Arch Pharm (Weinheim) 357 (6), e2400094 (2024)
   PUBMED   38631036
  REMARK    GeneRIF: What doesn't fit is made to fit: Pim-1 kinase adapts to
            the configuration of stilbene-based inhibitors.
REFERENCE   4  (bases 1 to 2703)
  AUTHORS   Xie,H.B., Guo,J.H., Yang,M.M. and Wang,J.T.
  TITLE     Kinase PIM1 governs ferroptosis to reduce retinal microvascular
            endothelial cell dysfunction triggered by high glucose
  JOURNAL   In Vitro Cell Dev Biol Anim 60 (3), 278-286 (2024)
   PUBMED   38485819
  REMARK    GeneRIF: Kinase PIM1 governs ferroptosis to reduce retinal
            microvascular endothelial cell dysfunction triggered by high
            glucose.
REFERENCE   5  (bases 1 to 2703)
  AUTHORS   Xie,Y., Xu,K., Dai,B., Guo,Z., Jiang,T., Chen,H. and Qiu,Y.
  TITLE     The 44 kDa Pim-1 kinase directly interacts with tyrosine kinase
            Etk/BMX and protects human prostate cancer cells from apoptosis
            induced by chemotherapeutic drugs
  JOURNAL   Oncogene 25 (1), 70-78 (2006)
   PUBMED   16186805
  REMARK    GeneRIF: PIM1 gene encodes two isoforms by alternative translation
            initiation at a non-AUG (CUG) and AUG start codons. The two
            isoforms differ in preferential cellular localization, and may
            regulate distinct substrates.
REFERENCE   6  (bases 1 to 2703)
  AUTHORS   Ragoussis,J., Senger,G., Mockridge,I., Sanseau,P., Ruddy,S.,
            Dudley,K., Sheer,D. and Trowsdale,J.
  TITLE     A testis-expressed Zn finger gene (ZNF76) in human 6p21.3
            centromeric to the MHC is closely linked to the human homolog of
            the t-complex gene tcp-11
  JOURNAL   Genomics 14 (3), 673-679 (1992)
   PUBMED   1427894
REFERENCE   7  (bases 1 to 2703)
  AUTHORS   Saris,C.J., Domen,J. and Berns,A.
  TITLE     The pim-1 oncogene encodes two related protein-serine/threonine
            kinases by alternative initiation at AUG and CUG
  JOURNAL   EMBO J 10 (3), 655-664 (1991)
   PUBMED   1825810
REFERENCE   8  (bases 1 to 2703)
  AUTHORS   Reeves,R., Spies,G.A., Kiefer,M., Barr,P.J. and Power,M.
  TITLE     Primary structure of the putative human oncogene, pim-1
  JOURNAL   Gene 90 (2), 303-307 (1990)
   PUBMED   2205533
REFERENCE   9  (bases 1 to 2703)
  AUTHORS   Amson,R., Sigaux,F., Przedborski,S., Flandrin,G., Givol,D. and
            Telerman,A.
  TITLE     The human protooncogene product p33pim is expressed during fetal
            hematopoiesis and in diverse leukemias
  JOURNAL   Proc Natl Acad Sci U S A 86 (22), 8857-8861 (1989)
   PUBMED   2682662
REFERENCE   10 (bases 1 to 2703)
  AUTHORS   Telerman,A., Amson,R., Zakut-Houri,R. and Givol,D.
  TITLE     Identification of the human pim-1 gene product as a 33-kilodalton
            cytoplasmic protein with tyrosine kinase activity
  JOURNAL   Mol Cell Biol 8 (4), 1498-1503 (1988)
   PUBMED   2837645
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA434260.1, DA481890.1,
            BC020224.1 and AL353579.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_002648.3.
            
            Summary: The protein encoded by this gene belongs to the Ser/Thr
            protein kinase family, and PIM subfamily. This gene is expressed
            primarily in B-lymphoid and myeloid cell lines, and is
            overexpressed in hematopoietic malignancies and in prostate cancer.
            It plays a role in signal transduction in blood cells, contributing
            to both cell proliferation and survival, and thus provides a
            selective advantage in tumorigenesis. Both the human and
            orthologous mouse genes have been reported to encode two isoforms
            (with preferential cellular localization) resulting from the use of
            alternative in-frame translation initiation codons, the upstream
            non-AUG (CUG) and downstream AUG codons (PMIDs:16186805,
            1825810).[provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) encodes two isoforms resulting
            from the use of alternate in-frame, translation initiation codons.
            This RefSeq represents the shorter isoform (2, also known as
            Pim-1S) derived from the use of a downstream AUG (at nt 431-433).
            Pim-1S has been shown to localize predominantly in the nucleus
            (PMID:16186805).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC020224.1, DQ022562.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373509.6/ ENSP00000362608.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               DA434260.1         7-129
            124-126             DA481890.1         23-25
            127-1690            BC020224.1         66-1629
            1691-2665           AL353579.17        30893-31867
            2666-2703           BC020224.1         2605-2642
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.2"
     gene            1..2703
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /note="Pim-1 proto-oncogene, serine/threonine kinase"
                     /db_xref="GeneID:5292"
                     /db_xref="HGNC:HGNC:8986"
                     /db_xref="MIM:164960"
     exon            1..506
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /inference="alignment:Splign:2.1.0"
     CDS             425..1366
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 1;
                     Oncogene PIM1; proto-oncogene serine/threonine-protein
                     kinase pim-1; pim-1 oncogene (proviral integration site
                     1)"
                     /codon_start=1
                     /product="serine/threonine-protein kinase pim-1 isoform 2"
                     /protein_id="NP_002639.1"
                     /db_xref="CCDS:CCDS4830.1"
                     /db_xref="GeneID:5292"
                     /db_xref="HGNC:HGNC:8986"
                     /db_xref="MIM:164960"
                     /translation="MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLL
                     GSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSG
                     VIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHN
                     CGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRY
                     HGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPS
                     DRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK"
     exon            507..613
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /inference="alignment:Splign:2.1.0"
     exon            614..664
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /inference="alignment:Splign:2.1.0"
     exon            665..1031
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1208
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..2703
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2683..2688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /note="hexamer: AATAAA"
     polyA_site      2703
                     /gene="PIM1"
                     /gene_synonym="PIM"
                     /note="major polyA site"
ORIGIN      
        1 actcctggct gcggggcgag ccgggcgtct gctgcagcgg ccgcggtggc tgaggaggcc
       61 cgagaggagt cggtggcagc ggcggcggcg ggaccggcag cagcagcagc agcagcagca
      121 gcagcaacca ctagcctcct gccccgcggc gctgccgcac gagccccacg agccgctcac
      181 cccgccgttc tcagcgctgc ccgaccccgc tggcgcgccc tcccgccgcc agtcccggca
      241 gcgccctcag ttgtcctccg actcgccctc ggccttccgc gccagccgca gccacagccg
      301 caacgccacc cgcagccaca gccacagcca cagccccagg catagccttc ggcacagccc
      361 cggctccggc tcctgcggca gctcctctgg gcaccgtccc tgcgccgaca tcctggaggt
      421 tgggatgctc ttgtccaaaa tcaactcgct tgcccacctg cgcgccgcgc cctgcaacga
      481 cctgcacgcc accaagctgg cgcccggcaa ggagaaggag cccctggagt cgcagtacca
      541 ggtgggcccg ctactgggca gcggcggctt cggctcggtc tactcaggca tccgcgtctc
      601 cgacaacttg ccggtggcca tcaaacacgt ggagaaggac cggatttccg actggggaga
      661 gctgcctaat ggcactcgag tgcccatgga agtggtcctg ctgaagaagg tgagctcggg
      721 tttctccggc gtcattaggc tcctggactg gttcgagagg cccgacagtt tcgtcctgat
      781 cctggagagg cccgagccgg tgcaagatct cttcgacttc atcacggaaa ggggagccct
      841 gcaagaggag ctggcccgca gcttcttctg gcaggtgctg gaggccgtgc ggcactgcca
      901 caactgcggg gtgctccacc gcgacatcaa ggacgaaaac atccttatcg acctcaatcg
      961 cggcgagctc aagctcatcg acttcgggtc gggggcgctg ctcaaggaca ccgtctacac
     1021 ggacttcgat gggacccgag tgtatagccc tccagagtgg atccgctacc atcgctacca
     1081 tggcaggtcg gcggcagtct ggtccctggg gatcctgctg tatgatatgg tgtgtggaga
     1141 tattcctttc gagcatgacg aagagatcat caggggccag gttttcttca ggcagagggt
     1201 ctcttcagaa tgtcagcatc tcattagatg gtgcttggcc ctgagaccat cagataggcc
     1261 aaccttcgaa gaaatccaga accatccatg gatgcaagat gttctcctgc cccaggaaac
     1321 tgctgagatc cacctccaca gcctgtcgcc ggggcccagc aaatagcagc ctttctggca
     1381 ggtcctcccc tctcttgtca gatgcccgag ggaggggaag cttctgtctc cagcttcccg
     1441 agtaccagtg acacgtctcg ccaagcagga cagtgcttga tacaggaaca acatttacaa
     1501 ctcattccag atcccaggcc cctggaggct gcctcccaac agtggggaag agtgactctc
     1561 caggggtcct aggcctcaac tcctcccata gatactctct tcttctcata ggtgtccagc
     1621 attgctggac tctgaaatat cccgggggtg gggggtgggg gtgggtcaga accctgccat
     1681 ggaactgttt ccttcatcat gagttctgct gaatgccgcg atgggtcagg taggggggaa
     1741 acaggttggg atgggatagg actagcacca ttttaagtcc ctgtcacctc ttccgactct
     1801 ttctgagtgc cttctgtggg gactccggct gtgctgggag aaatacttga acttgcctct
     1861 tttacctgct gcttctccaa aaatctgcct gggttttgtt ccctattttt ctctcctgtc
     1921 ctccctcacc ccctccttca tatgaaaggt gccatggaag aggctacagg gccaaacgct
     1981 gagccacctg cccttttttc tgcctccttt agtaaaactc cgagtgaact ggtcttcctt
     2041 tttggttttt acttaactgt ttcaaagcca agacctcaca cacacaaaaa atgcacaaac
     2101 aatgcaatca acagaaaagc tgtaaatgtg tgtacagttg gcatggtagt atacaaaaag
     2161 attgtagtgg atctaatttt taagaaattt tgcctttaag ttattttacc tgtttttgtt
     2221 tcttgttttg aaagatgcgc attctaacct ggaggtcaat gttatgtatt tatttattta
     2281 tttatttggt tcccttccta ttccaagctt ccatagctgc tgccctagtt ttctttcctc
     2341 ctttcctcct ctgacttggg gaccttttgg gggagggctg cgacgcttgc tctgtttgtg
     2401 gggtgacggg actcaggcgg gacagtgctg cagctccctg gcttctgtgg ggcccctcac
     2461 ctacttaccc aggtgggtcc cggctctgtg ggtgatgggg aggggcattg ctgactgtgt
     2521 atataggata attatgaaaa gcagttctgg atggtgtgcc ttccagatcc tctctggggc
     2581 tgtgttttga gcagcaggta gcctgctggt tttatctgag tgaaatactg tacaggggaa
     2641 taaaagagat cttatttttt tttttatact tggcgttttt tgaataaaaa ccttttgtct
     2701 taa
//
