LOCUS       NM_015040               9908 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens phosphoinositide kinase, FYVE-type zinc finger
            containing (PIKFYVE), transcript variant 2, mRNA.
ACCESSION   NM_015040 XM_028867
VERSION     NM_015040.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Zhao,M., Zhang,S., Wan,W., Zhou,C., Li,N., Cheng,R., Yu,Y.,
            Ouyang,X., Zhou,D., Jiao,J. and Xiong,X.
  TITLE     Coxiella burnetii effector CvpE maintains biogenesis of
            Coxiella-containing vacuoles by suppressing lysosome tubulation
            through binding PI(3)P and perturbing PIKfyve activity on lysosomes
  JOURNAL   Virulence 15 (1), 2350893 (2024)
   PUBMED   38725096
  REMARK    GeneRIF: Coxiella burnetii effector CvpE maintains biogenesis of
            Coxiella-containing vacuoles by suppressing lysosome tubulation
            through binding PI(3)P and perturbing PIKfyve activity on
            lysosomes.
REFERENCE   2  (bases 1 to 9908)
  AUTHORS   Saffi,G.T., To,L., Kleine,N., Melo,C.M.P., Chen,K., Genc,G.,
            Lee,K.C.D., Chow,J.T., Jang,G.H., Gallinger,S., Botelho,R.J. and
            Salmena,L.
  TITLE     INPP4B promotes PDAC aggressiveness via PIKfyve and
            TRPML-1-mediated lysosomal exocytosis
  JOURNAL   J Cell Biol 223 (11) (2024)
   PUBMED   39120584
  REMARK    GeneRIF: INPP4B promotes PDAC aggressiveness via PIKfyve and
            TRPML-1-mediated lysosomal exocytosis.
REFERENCE   3  (bases 1 to 9908)
  AUTHORS   Choi,J.E., Qiao,Y., Kryczek,I., Yu,J., Gurkan,J., Bao,Y.,
            Gondal,M., Tien,J.C., Maj,T., Yazdani,S., Parolia,A., Xia,H.,
            Zhou,J., Wei,S., Grove,S., Vatan,L., Lin,H., Li,G., Zheng,Y.,
            Zhang,Y., Cao,X., Su,F., Wang,R., He,T., Cieslik,M., Green,M.D.,
            Zou,W. and Chinnaiyan,A.M.
  TITLE     PIKfyve, expressed by CD11c-positive cells, controls tumor immunity
  JOURNAL   Nat Commun 15 (1), 5487 (2024)
   PUBMED   38942798
  REMARK    GeneRIF: PIKfyve, expressed by CD11c-positive cells, controls tumor
            immunity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9908)
  AUTHORS   Ferrarone,J.R., Thomas,J., Unni,A.M., Zheng,Y., Nagiec,M.J.,
            Gardner,E.E., Mashadova,O., Li,K., Koundouros,N., Montalbano,A.,
            Mustafa,M., Cantley,L.C., Blenis,J., Sanjana,N.E. and Varmus,H.
  TITLE     Genome-wide CRISPR screens in spheroid culture reveal that the
            tumor suppressor LKB1 inhibits growth via the PIKFYVE lipid kinase
  JOURNAL   Proc Natl Acad Sci U S A 121 (21), e2403685121 (2024)
   PUBMED   38743625
  REMARK    GeneRIF: Genome-wide CRISPR screens in spheroid culture reveal that
            the tumor suppressor LKB1 inhibits growth via the PIKFYVE lipid
            kinase.
REFERENCE   5  (bases 1 to 9908)
  AUTHORS   Klein,A.D., Petruzzi,K.L., Lee,C. and Overholtzer,M.
  TITLE     Stress-induced microautophagy is coordinated with lysosome
            biogenesis and regulated by PIKfyve
  JOURNAL   Mol Biol Cell 35 (5), ar70 (2024)
   PUBMED   38536415
  REMARK    GeneRIF: Stress-induced microautophagy is coordinated with lysosome
            biogenesis and regulated by PIKfyve.
REFERENCE   6  (bases 1 to 9908)
  AUTHORS   Ikonomov,O.C., Sbrissa,D., Mlak,K., Deeb,R., Fligger,J., Soans,A.,
            Finley,R.L. Jr. and Shisheva,A.
  TITLE     Active PIKfyve associates with and promotes the membrane attachment
            of the late endosome-to-trans-Golgi network transport factor Rab9
            effector p40
  JOURNAL   J Biol Chem 278 (51), 50863-50871 (2003)
   PUBMED   14530284
  REMARK    GeneRIF: p40 interaction with PIKfyve p40 os demonstrated; PIKfyve
            interaction and the subsequent PIKfyve-catalyzed p40
            phosphorylation anchor p40 to discrete membranes facilitating late
            endosome-to-TGN transport.
REFERENCE   7  (bases 1 to 9908)
  AUTHORS   Jiao,X., Munier,F.L., Schorderet,D.F., Zografos,L., Smith,J.,
            Rubin,B. and Hejtmancik,J.F.
  TITLE     Genetic linkage of Francois-Neetens fleck (mouchetee) corneal
            dystrophy to chromosome 2q35
  JOURNAL   Hum Genet 112 (5-6), 593-599 (2003)
   PUBMED   12607114
REFERENCE   8  (bases 1 to 9908)
  AUTHORS   Sbrissa,D., Ikonomov,O.C., Deeb,R. and Shisheva,A.
  TITLE     Phosphatidylinositol 5-phosphate biosynthesis is linked to PIKfyve
            and is involved in osmotic response pathway in mammalian cells
  JOURNAL   J Biol Chem 277 (49), 47276-47284 (2002)
   PUBMED   12270933
REFERENCE   9  (bases 1 to 9908)
  AUTHORS   Ikonomov,O.C., Sbrissa,D., Mlak,K., Kanzaki,M., Pessin,J. and
            Shisheva,A.
  TITLE     Functional dissection of lipid and protein kinase signals of
            PIKfyve reveals the role of PtdIns 3,5-P2 production for
            endomembrane integrity
  JOURNAL   J Biol Chem 277 (11), 9206-9211 (2002)
   PUBMED   11714711
REFERENCE   10 (bases 1 to 9908)
  AUTHORS   Sbrissa,D., Ikonomov,O.C. and Shisheva,A.
  TITLE     Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve.
            Binding specificity and role in PIKfyve. Endomenbrane localization
  JOURNAL   J Biol Chem 277 (8), 6073-6079 (2002)
   PUBMED   11706043
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016697.8 and AC012362.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015040.3.
            
            Summary: Phosphorylated derivatives of phosphatidylinositol
            (PtdIns) regulate cytoskeletal functions, membrane trafficking, and
            receptor signaling by recruiting protein complexes to cell- and
            endosomal-membranes. Humans have multiple PtdIns proteins that
            differ by the degree and position of phosphorylation of the
            inositol ring. This gene encodes an enzyme (PIKfyve; also known as
            phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that
            phosphorylates the D-5 position in PtdIns and
            phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and
            PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated
            by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct
            genes and preferentially phosphorylate D-4 phosphorylated PtdIns.
            In contrast, PIKfyve preferentially phosphorylates D-3
            phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve
            also has protein kinase activity. PIKfyve regulates endomembrane
            homeostasis and plays a role in the biogenesis of endosome carrier
            vesicles from early endosomes. The protein plays a key role in cell
            entry of ebola virus and SARS-CoV-2 by endocytosis Mutations in
            this gene cause corneal fleck dystrophy (CFD); an autosomal
            dominant disorder characterized by numerous small white flecks
            present in all layers of the corneal stroma. Histologically, these
            flecks appear to be keratocytes distended with lipid and
            mucopolysaccharide filled intracytoplasmic vacuoles. [provided by
            RefSeq, Jul 2021].
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY457063.1, EU794609.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in SARS-CoV-2 infection
            MANE Ensembl match     :: ENST00000264380.9/ ENSP00000264380.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-161               AC016697.8         124188-124348
            162-342             AC016697.8         129444-129624
            343-492             AC016697.8         131517-131666
            493-611             AC016697.8         134645-134763
            612-783             AC016697.8         135470-135641
            784-991             AC016697.8         143659-143866
            992-1081            AC016697.8         146662-146751
            1082-1220           AC016697.8         156574-156712
            1221-1378           AC016697.8         158870-159027
            1379-1490           AC016697.8         160175-160286
            1491-1638           AC012362.7         615-762
            1639-1806           AC012362.7         1290-1457
            1807-1866           AC012362.7         8680-8739
            1867-1996           AC012362.7         10738-10867
            1997-2177           AC012362.7         11637-11817
            2178-2252           AC012362.7         14311-14385
            2253-2360           AC012362.7         16696-16803
            2361-2501           AC012362.7         20586-20726
            2502-2628           AC012362.7         21355-21481
            2629-3788           AC012362.7         21714-22873
            3789-3889           AC012362.7         24624-24724
            3890-3961           AC012362.7         26286-26357
            3962-4133           AC012362.7         26967-27138
            4134-4312           AC012362.7         29759-29937
            4313-4426           AC012362.7         31750-31863
            4427-4535           AC012362.7         32237-32345
            4536-4690           AC012362.7         32490-32644
            4691-4781           AC012362.7         33282-33372
            4782-4842           AC012362.7         34952-35012
            4843-4980           AC012362.7         35862-35999
            4981-5101           AC012362.7         36455-36575
            5102-5197           AC012362.7         38998-39093
            5198-5281           AC012362.7         41555-41638
            5282-5379           AC012362.7         42494-42591
            5380-5544           AC012362.7         44303-44467
            5545-5604           AC012362.7         46468-46527
            5605-5781           AC012362.7         47215-47391
            5782-5885           AC012362.7         47796-47899
            5886-6014           AC012362.7         49098-49226
            6015-6276           AC012362.7         50342-50603
            6277-6351           AC012362.7         51015-51089
            6352-9908           AC012362.7         51634-55190
FEATURES             Location/Qualifiers
     source          1..9908
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q34"
     gene            1..9908
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /note="phosphoinositide kinase, FYVE-type zinc finger
                     containing"
                     /db_xref="GeneID:200576"
                     /db_xref="HGNC:HGNC:23785"
                     /db_xref="MIM:609414"
     exon            1..161
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            162..342
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..6467
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.1.150"
                     /note="isoform 2 is encoded by transcript variant 2;
                     phosphatidylinositol-3-phosphate/phosphatidylinositol
                     5-kinase, type III; phosphatidylinositol 3-phosphate
                     5-kinase type III; 1-phosphatidylinositol 3-phosphate
                     5-kinase; zinc finger, FYVE domain containing 29;
                     epididymis luminal protein 37; PIPkin-III; type III PIP
                     kinase; phosphoinositide kinase, FYVE finger containing;
                     serine-protein kinase PIKFYVE"
                     /codon_start=1
                     /product="1-phosphatidylinositol 3-phosphate 5-kinase
                     isoform 2"
                     /protein_id="NP_055855.2"
                     /db_xref="CCDS:CCDS2382.1"
                     /db_xref="GeneID:200576"
                     /db_xref="HGNC:HGNC:23785"
                     /db_xref="MIM:609414"
                     /translation="MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKS
                     AYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQR
                     RSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQ
                     CKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIAL
                     SYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLI
                     HPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANR
                     TYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVN
                     WLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSP
                     DSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVD
                     SDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKE
                     SLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLL
                     HSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVN
                     GFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQ
                     RIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQL
                     LTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEIL
                     IFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWD
                     PDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFF
                     APVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEE
                     VTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWS
                     PLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQ
                     SLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWS
                     TKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYC
                     FRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICK
                     QVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVA
                     SFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKT
                     DTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKK
                     QSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGL
                     QNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHL
                     LGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPS
                     SIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQT
                     NRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTE
                     NQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSE
                     EDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITN
                     AVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRN
                     RNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIID
                     YSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRT
                     RFCEAMDKYFLMVPDHWTGLGLNC"
     exon            343..492
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            493..611
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            612..783
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            784..991
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1081
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1220
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1378
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1490
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1638
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1806
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1866
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1867..1996
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            1997..2177
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2252
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2360
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..2501
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2628
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            2629..3788
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            3789..3889
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            3890..3961
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            3962..4133
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4134..4312
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4313..4426
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4427..4535
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4536..4690
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4691..4781
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4782..4842
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4843..4980
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            4981..5101
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5102..5197
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5198..5281
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5282..5379
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5380..5544
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5545..5604
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5605..5781
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5782..5885
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            5886..6014
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            6015..6276
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            6277..6351
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     exon            6352..9908
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9888..9893
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /note="hexamer: AATAAA"
     polyA_site      9908
                     /gene="PIKFYVE"
                     /gene_synonym="CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29"
                     /note="major polyA site"
ORIGIN      
        1 gagaagccgc atcaaccatg taagcagctt cgcttcctgc cgcaaccgtc cgcggcctga
       61 ggagcccacc gccgctctcg ggggccgact tccgggggct gagccgttga agcggaggct
      121 ggggcggggg gcagccggcg cggccggggc aggaggcgca gactcatgaa atggccacag
      181 atgataagac gtccccaaca ctggactctg ctaatgattt gcctcgatct cctactagtc
      241 cttctcatct cacacacttt aaacctttga ctcctgatca agatgagccc ccttttaaat
      301 cagcttatag ttcttttgta aatctctttc gttttaacaa agagagagca gaaggaggcc
      361 agggagaaca gcagcctttg agtggaagtt ggaccagccc tcagctccct tcgaggacac
      421 agtctgttag gtcacccaca ccttataaaa agcagcttaa tgaggaactc cagcggcgct
      481 cttcagcatt agacacaaga aggaaagcag aacctacctt tggaggtcat gaccctcgta
      541 cagctgttca gcttcgaagc ctcagcacag tattaaaacg cctcaaggaa atcatggagg
      601 ggaaaagcca ggatagtgac ctgaaacaat actggatgcc agatagccaa tgtaaagagt
      661 gctatgactg tagtgagaaa tttacaacct ttaggcgcag acaccattgc cgactatgtg
      721 ggcagatttt ctgcagtcgt tgctgtaatc aagaaatccc tggaaaattt atgggctata
      781 caggagacct ccgagcttgc acatattgta gaaaaatagc cttaagttat gctcattcca
      841 cagacagtaa ttctattggg gaagacttga atgctctttc agattctgct tgctctgtgt
      901 ctgtgcttga tccaagtgaa ccccgaacac ctgttgggag taggaaagcc agccgtaaca
      961 tatttttaga ggatgatttg gcctggcaaa gtttgattca tccagattcc tcaaatactc
     1021 ctctttcaac aagacttgta tctgtgcaag aggatgctgg gaaatctcct gctcgaaata
     1081 gatcagccag cattactaac ctgtcactgg atagatctgg ttctcctatg gtaccttcat
     1141 atgagacatc tgtcagtccc caggctaacc gaacatatgt taggacagag accactgagg
     1201 atgaacgcaa aattcttctg gacagtgtgc agttaaaaga cctgtggaaa aaaatctgcc
     1261 atcacagcag tggaatggag tttcaggatc accgctactg gttgagaacg catcccaact
     1321 gcattgtagg aaaggaatta gtcaactggc taatccgaaa tgggcatatt gccacaaggg
     1381 cacaagctat agcaattgga caagcaatgg ttgatggacg ttggctggat tgtgttagtc
     1441 atcacgacca gcttttcaga gatgagtatg cgctgtatag accactgcag agtacagaat
     1501 tttctgagac gccttctccc gacagtgact cagtgaactc cgtggaagga cactctgagc
     1561 catcctggtt taaagacata aagtttgatg acagtgacac agaacagata gctgaagaag
     1621 gtgacgataa tttggctaat tctgccagtc ctagcaagcg cacatcagtc agcagtttcc
     1681 agtccacagt ggacagtgac tcagccgctt ctatcagcct gaacgtggag ctggacaacg
     1741 tgaacttcca tatcaagaag ccctccaagt acccacatgt gccccctcac cctgctgacc
     1801 aaaaagagta tttgatttct gacactggag gacaacagct ctcaataagt gacgctttca
     1861 tcaaagaatc cttatttaat cgccgagtag aggaaaaatc caaagagctg cctttcacac
     1921 ctttgggctg gcatcataac aacctggagc tcctgaggga ggagaatggg gagaaacaag
     1981 ccatggagag gttgctttca gctaatcata accacatgat ggcactactc cagcagttgc
     2041 tccatagtga ctcactgtca tcatcttgga gggacatcat cgtgtcattg gtctgccagg
     2101 ttgttcagac agtccgacct gatgtcaaga accaggatga tgacatggat atccgtcagt
     2161 ttgtccacat caaaaaaatc ccaggtggaa agaagtttga ttctgtggtt gtcaatggct
     2221 ttgtttgtac caagaacatt gcacataaaa agatgagttc ttgtattaaa aaccccaaaa
     2281 ttcttctgtt gaagtgttcc attgagtatc tctacagaga agaaactaag tttacttgca
     2341 ttgatcctat tgtgcttcag gaaagggaat tcttgaagaa ttatgtccag cgaatagttg
     2401 atgttcgacc caccttggtt cttgttgaga aaacagtgtc tcggattgcc caggacatgt
     2461 tattggaaca tggcattact ttggtcatta atgtaaagtc acaagttttg gaacgaatca
     2521 gtcgaatgac ccaaggtgat ttagtgatgt caatggacca gctgcttacg aaaccacacc
     2581 tgggcacttg tcacaaattt tatatgcaga tatttcagtt gcctaatgaa caaaccaaga
     2641 cactgatgtt ttttgaaggt tgtccacagc acctaggctg tacaatcaag ctaagaggag
     2701 gctctgatta tgagctggct cgagttaagg agatcctaat atttatgatc tgtgttgctt
     2761 atcattctca actagaaata tcctttctca tggatgaatt tgctatgcct cccacattaa
     2821 tgcaaaaccc ttcattccat tccctgattg agggacgagg gcatgagggg gctgtccaag
     2881 agcagtacgg tggaggttcc atcccctggg atcctgacat ccctcctgag tctctgccct
     2941 gtgatgatag cagtttgctg gaattgagga ttgtgtttga gaagggtgag caggaaaata
     3001 aaaatcttcc gcaggctgtt gcctctgtga agcatcaaga acatagcaca acagcttgcc
     3061 cggcgggtct cccttgtgct ttctttgcac ctgtaccgga atcattgttg ccactccctg
     3121 tggatgacca acaagatgct ttaggcagcg agcagccaga gactttgcag caaacagttg
     3181 tgctgcagga tcccaaaagc cagataagag cctttagaga ccctctacag gatgacactg
     3241 gattatatgt tactgaggaa gtcacctcct ctgaagataa acgaaagact tattctttgg
     3301 cctttaagca ggaattaaaa gatgtgatcc tctgtatctc cccagtaatc acattccgag
     3361 aaccctttct tttaactgaa aaggggatga gatgctctac ccgagattat tttgcagagc
     3421 aggtttactg gtctcctctc ctcaataaag aattcaaaga aatggagaac aggaggaaga
     3481 aacagctgct cagggatctc tctggacttc agggcatgaa tggaagtatt caggccaagt
     3541 ctattcaagt cttaccctca catgagctag tgagcactag aattgctgag catctgggcg
     3601 atagccagag cttgggtaga atgctggccg attatcgagc cagaggagga agaattcagc
     3661 ccaaaaattc agaccctttt gctcattcaa aggatgcatc aagtacttca agtggccaat
     3721 caggaagcaa aaatgagggt gatgaagaga gagggcttat tctgagtgat gctgtgtggt
     3781 caacaaaggt ggactgtctg aatcccatta atcaccagag actttgtgtg ctcttcagca
     3841 gctcttctgc ccagtccagc aatgctccta gtgcctgtgt cagtccttgg attgtaacaa
     3901 tggaatttta tggaaagaat gatcttacat taggaatatt tttagagaga tactgtttca
     3961 ggccttctta tcagtgtcca agcatgttct gtgatacccc catggtacat catattcggc
     4021 gctttgttca tggccaaggc tgtgtgcaga taatcctgaa ggagttggat tctccagtac
     4081 ctggatatca gcatacaatt cttacatatt cctggtgtag aatctgcaaa caggtaacac
     4141 cagttgttgc tctttccaat gagtcctggt ctatgtcatt tgcaaaatac cttgaactta
     4201 ggttttatgg gcaccagtat actcgcagag ccaacgctga gccctgtggt cactccatcc
     4261 atcatgatta tcaccagtat ttctcctata accagatggt ggcgtctttc agttattctc
     4321 ccattcggct tcttgaagta tgtgttccac tccccaaaat attcattaag cgtcaggccc
     4381 cattaaaagt gtcccttctt caggatctga aggacttctt tcaaaaagtt tcacaggtat
     4441 atgttgccat tgatgaaaga cttgcatctt tgaaaactga tacatttagt aaaacaagag
     4501 aggaaaaaat ggaagatatt tttgcacaga aagagatgga agaaggtgag ttcaagaact
     4561 ggattgagaa gatgcaagca aggctcatgt cttcctctgt agatacccct cagcaactgc
     4621 agtcggtctt tgagtcactc attgccaaga aacaaagtct ctgtgaagtg ctgcaagctt
     4681 ggaataacag gttgcaggac cttttccaac aggaaaaggg tagaaagaga ccttcagttc
     4741 ctccaagtcc tggaagactg agacaagggg aagaaagcaa gataagtgcg atggatgcat
     4801 ctccacggaa tatttctcca ggacttcaga atggagaaaa agaggatcgc ttcttaacaa
     4861 ctttgtccag ccagagctcc accagttcta ctcatctcca attgcctacg ccacctgaag
     4921 tcatgtctga acagtcagtg ggagggcccc ctgagctaga tacagccagc agttccgaag
     4981 atgtgtttga tgggcatttg ctgggatcca cagacagcca agtgaaggaa aagtcaacca
     5041 tgaaagccat ctttgcaaat ttgcttccag gaaatagcta taatcctatt ccatttcctt
     5101 ttgatccaga taaacactac ttaatgtatg aacatgaacg agtgcccatt gcagtctgcg
     5161 agaaggaacc cagctccatc attgcttttg ctctcagttg taaagaatac cgaaatgcct
     5221 tagaggaatt gtctaaagcg actcagtgga acagtgccga agaagggctt ccaacaaata
     5281 gtacttcaga tagcagacca aagagtagca gccctatcag attacctgaa atgagtggag
     5341 gacagacaaa tcgtacaaca gaaacagaac cacaaccaac caaaaaggct tctggaatgt
     5401 tgtccttctt cagagggaca gcagggaaaa gccccgatct ctcttcccag aagagagaga
     5461 ccttacgtgg agcagatagt gcttactacc aggttgggca gacgggcaag gaggggaccg
     5521 agaatcaagg cgttgagcct caagatgaag tagatggagg agatacacaa aagaagcaac
     5581 tcataaatcc tcatgtggaa cttcaatttt cagatgctaa tgccaagttt tactgtcggc
     5641 tctactatgc gggagagttt cataagatgc gtgaagtgat tctggacagc agtgaagaag
     5701 atttcattcg ttccctctcc cactcatcac cctggcaggc ccggggaggc aaatcaggag
     5761 ctgccttcta tgcaactgag gatgatagat ttattttgaa gcaaatgcct cgtctggaag
     5821 tccagtcctt cctcgacttt gcaccacatt acttcaatta tattacaaat gctgttcaac
     5881 aaaagaggcc cacggcgttg gccaaaattc ttggagttta cagaattggt tataagaact
     5941 ctcagaacaa cactgagaag aagttagatc tccttgtcat ggaaaatctt ttctacggga
     6001 gaaagatggc acaggttttt gatttgaagg gctctcttag gaatcggaat gtaaaaactg
     6061 acactggaaa agagagttgt gatgtggtcc tgctagatga aaatctccta aagatggttc
     6121 gagacaaccc tctatatatt cgttctcatt ccaaagctgt gctgagaacc tcgatccata
     6181 gtgactccca tttcctttct agccacctca ttatagatta ttctttgctg gttgggcgag
     6241 atgatactag caatgagcta gtagttggaa ttatagatta tattcgaaca tttacatggg
     6301 acaaaaagct tgagatggtt gtgaaatcaa caggaatttt aggtggacaa ggtaaaatgc
     6361 caacagtggt gtctccggag ttgtacagga ctaggttttg tgaggcaatg gacaagtatt
     6421 tcctaatggt accagaccac tggacaggct tgggtctgaa ttgctgaaat caagcacata
     6481 ttttgaaatg gactgtgaag gaaaagggga caggaacaaa ggaccaaaaa taagctacat
     6541 gttttatttc ttcatcgtgt tcaccactgt atgccaaggc ttttcagttc tgtggctgtt
     6601 tagactgtcc gtaatggaat ggtaaaactc catgaatttg cactttggtt tttgatacct
     6661 gtggagctgt ctgtaggttg ggaagtggca tgaaaatttt cttaagctaa aatacagaca
     6721 tgtttcaaag ggctaaagtt ggagatgagt agatagggtg aaaaatgggt taaatttgct
     6781 agcttaattg ttttaagaag aaaacagtgt ctcataaatt gactatcctg gcatcacatt
     6841 taacatgtta tctacttaga aagcatttgt agagctgctg aatttgtttt gtgtttttct
     6901 gtaataattt aatgttactt attatcagaa tttctgaaac ctttacaaaa attctgattt
     6961 attccattaa tggccggtta aacacgtggg catttattgt tttattgagg aatttgactt
     7021 aaactgggaa tcctgtcatg ttgtttatct ttccagcttg cctgtttttg agtatgtttg
     7081 atgtttttaa aattttgtct tctctgtgga agacaggagg ctacagcaat taactttaag
     7141 cctcctttta gagatatttt taaaacttgt ttaaaatttt tgtgcaattc atatattaaa
     7201 ttgcacttac ttgcatacgc tcatattcta gggttttttc tctattttta gggtatcata
     7261 gtaaatcatt agtaaatgag tctgtagtta ctaaacccta atggaataat tattaatgaa
     7321 agatttttga aatataaaaa ataaattagg cccaatccaa gaaattgagt gagaaggaaa
     7381 cacttgtttt attcacagag gtaaagtgtc ttttcaatat aaccagcaat ttaggtggca
     7441 tctataaaat aaaaaatttc tactgtggac atcccctttt ccaactttct acataatggc
     7501 tagttctgac tactaagaaa tgttaagaaa taggccaagt gcggtggctc acgcctataa
     7561 tcctatcact ttgggaggcc taggcaggca gatcacctga ggtcaggagt tcaagaccag
     7621 cctggccaac atgacgaaac cccatctcta ctaaaaagac aaaaaattag ctgggcatgg
     7681 tggcatatac ctgtaacccc aactacttgg gtggctgagg caggagaatt gtttgaacct
     7741 gggaggcgga ggctgcagtg agctgggatt acaccattgc attccagcct gggcaacaga
     7801 gcaagactgt ctcaaaaaac aaaacgaaac aaaaaaagaa agttattctt agtaaggaac
     7861 ttcttgttta atagcatttt tgtttatttt aaaaagtgat cagaagtagt aaactatctt
     7921 tgaggaaata ctgtaacccc agaatatttc ctcttgactt ctttttgtaa caaggataat
     7981 ttagggattt ataaagttgt aaggatttca ctgtttttgg actgcctata ataatagcac
     8041 attaaccttc acataataag aaatctggac aagttcagtt acacagtatg atgaatactt
     8101 gaattaggaa cattgtggaa aatttgcttt agagaatcaa ggcagtagtt tggtatttgg
     8161 tgcttattaa aaatgtggtt tgttttgaac tggaagcaag ttgaccaagg acttatgact
     8221 aatgtgatgc taagttccac ttggcccctt ttaaaaacgt gtatgtgcct tttgaagata
     8281 cacaaaacac tgaggatttt agttttgaaa tcaaagacta ttaaaggagc tgtacagagg
     8341 taaaaaaata aatgtggaac attattaact tactttgtgt ctaggaacaa tggattttgt
     8401 atctgattta aaatgccaac actgttttgt ctctgttcat tttttctgtg aggatactta
     8461 aggttattat tcctgtctgt ttcctgtact cccctagtca tgagcacttg aagtacaagg
     8521 tgtctccccc taggtgcaat taggttgttt ctttgttttt agtttcaatt ctatgtgcat
     8581 agcaggaatg ctccacagga atggcttctg acaataatct gtcctgttga ttttgttttc
     8641 cttgcccatg acttgaacaa ctgtgtttta aagtactgta gtctagtagg taactttgtg
     8701 gcaaaaattt tcaatataat acattctgaa acaatagttg ctgccttgca aaggtaatct
     8761 ctcattttaa aattggacag tattaatgaa ggggaaatat acaatttatt tctattgagt
     8821 ggtagaacta tatgtctggt cccttgctgc tcttgtttag gccactatca tagatatatt
     8881 tcaaatattg tactactcag tgttaagtat tgaatgactg tttccctttc cttcaaggcc
     8941 tagagtatat tctgaaaatt taggaatgag gaagaaatct taatacttcc ttccttaaca
     9001 tacaacatga gtcccgagaa taattgatag tagcaagaga aaactatgtc agtaacatgt
     9061 tgctttgtat aaaaatctta tttataaatg tgaagctttt tgatgccatc aaaacttatt
     9121 aaaaaatagg atttactttt ttctaattct gacctaagaa aaataatgag aacaagctgt
     9181 tgcaagctct tttgtagtct attgaatatt ttatagatat tcaaaatttc ctacaaacta
     9241 taattttttc catgatttag cagtgagtga ttttctagct ttggctctta ttaggtattg
     9301 taaatagtag ggttatatcg atatcagctt ttgtgatggc attgtggtca tcagcttcat
     9361 gacattttac ccatttgcag tgatcctgtg taaaactgcc aaggaaagta attacctgta
     9421 ggagtttgct gagcttgaag agtgaaaact gttgtgaatg agcctgatca taaaacggac
     9481 caggccattc attattcctc aagtgttaat atactgactt atgcagtatt caaaccatct
     9541 agtgcaatgt ttttgttttt gttttttttt tggtaacaca ggtgcagtgt attatagaaa
     9601 aaataaaaac tacaatcatt agcagtttta atactgctgt gtcagttttg taaaaaatgt
     9661 acattatgtc ttttgacatg ttgaatttta aactagggaa atgacattgt aaatcatagt
     9721 agcctctttt aatttaatat gaaaaatgcc actatattga aagtacttaa tgtattgtat
     9781 atatttctct actttggttc tagctatttt atatgattga catgttattt aaaagataac
     9841 tgccttgaac ttttggagac ttgtactgta aataaagaaa tcttaacaat aaactcagaa
     9901 tctactta
//
