LOCUS       NM_001142633            4491 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens phosphoinositide-3-kinase regulatory subunit 5
            (PIK3R5), transcript variant 1, mRNA.
ACCESSION   NM_001142633
VERSION     NM_001142633.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Luo,Q., Raulston,E.G., Prado,M.A., Wu,X., Gritsman,K., Whalen,K.S.,
            Yan,K., Booth,C.A.G., Xu,R., van Galen,P., Doench,J.G., Shimony,S.,
            Long,H.W., Neuberg,D.S., Paulo,J.A. and Lane,A.A.
  TITLE     Targetable leukaemia dependency on noncanonical PI3Kgamma
            signalling
  JOURNAL   Nature 630 (8015), 198-205 (2024)
   PUBMED   38720074
  REMARK    GeneRIF: Targetable leukaemia dependency on noncanonical PI3Kgamma
            signalling.
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Quintanilha,J.C.F., Racioppi,A., Wang,J., Etheridge,A.S.,
            Denning,S., Pena,C.E., Skol,A.D., Crona,D.J., Lin,D. and
            Innocenti,F.
  TITLE     PIK3R5 genetic predictors of hypertension induced by VEGF-pathway
            inhibitors
  JOURNAL   Pharmacogenomics J 22 (1), 82-88 (2022)
   PUBMED   34775477
  REMARK    GeneRIF: PIK3R5 genetic predictors of hypertension induced by
            VEGF-pathway inhibitors.
            Erratum:[Pharmacogenomics J. 2022 Mar;22(2):145. doi:
            10.1038/s41397-021-00264-2.. PMID: 34934177]
REFERENCE   3  (bases 1 to 4491)
  AUTHORS   Liu,Y., Jing,X.B., Wang,Z.C. and Han,Q.K.
  TITLE     HCP5, as the sponge of miR-1291, facilitates AML cell proliferation
            and restrains apoptosis via increasing PIK3R5 expression
  JOURNAL   Hum Genomics 15 (1), 38 (2021)
   PUBMED   34187569
  REMARK    GeneRIF: HCP5, as the sponge of miR-1291, facilitates AML cell
            proliferation and restrains apoptosis via increasing PIK3R5
            expression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4491)
  AUTHORS   Khater,M., Wei,Z., Xu,X., Huang,W., Lokeshwar,B.L., Lambert,N.A.
            and Wu,G.
  TITLE     G protein betagamma translocation to the Golgi apparatus activates
            MAPK via p110gamma-p101 heterodimers
  JOURNAL   J Biol Chem 296, 100325 (2021)
   PUBMED   33493514
  REMARK    GeneRIF: G protein betagamma translocation to the Golgi apparatus
            activates MAPK via p110gamma-p101 heterodimers.
REFERENCE   5  (bases 1 to 4491)
  AUTHORS   Nurnberg,B. and Beer-Hammer,S.
  TITLE     Function, Regulation and Biological Roles of PI3Kgamma Variants
  JOURNAL   Biomolecules 9 (9), 427 (2019)
   PUBMED   31480354
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4491)
  AUTHORS   Voigt,P., Brock,C., Nurnberg,B. and Schaefer,M.
  TITLE     Assigning functional domains within the p101 regulatory subunit of
            phosphoinositide 3-kinase gamma
  JOURNAL   J Biol Chem 280 (6), 5121-5127 (2005)
   PUBMED   15611065
  REMARK    GeneRIF: analysis of functional domains in the p101 regulatory
            subunit of phosphoinositide 3-kinase gamma
REFERENCE   7  (bases 1 to 4491)
  AUTHORS   Brock,C., Schaefer,M., Reusch,H.P., Czupalla,C., Michalke,M.,
            Spicher,K., Schultz,G. and Nurnberg,B.
  TITLE     Roles of G beta gamma in membrane recruitment and activation of
            p110 gamma/p101 phosphoinositide 3-kinase gamma
  JOURNAL   J Cell Biol 160 (1), 89-99 (2003)
   PUBMED   12507995
  REMARK    GeneRIF: p101 has a role in membrane recruitment and activation of
            PI3K gamma
REFERENCE   8  (bases 1 to 4491)
  AUTHORS   Metjian,A., Roll,R.L., Ma,A.D. and Abrams,C.S.
  TITLE     Agonists cause nuclear translocation of phosphatidylinositol
            3-kinase gamma. A Gbetagamma-dependent pathway that requires the
            p110gamma amino terminus
  JOURNAL   J Biol Chem 274 (39), 27943-27947 (1999)
   PUBMED   10488142
REFERENCE   9  (bases 1 to 4491)
  AUTHORS   Krugmann,S., Hawkins,P.T., Pryer,N. and Braselmann,S.
  TITLE     Characterizing the interactions between the two subunits of the
            p101/p110gamma phosphoinositide 3-kinase and their role in the
            activation of this enzyme by G beta gamma subunits
  JOURNAL   J Biol Chem 274 (24), 17152-17158 (1999)
   PUBMED   10358071
REFERENCE   10 (bases 1 to 4491)
  AUTHORS   Stoyanov,B., Volinia,S., Hanck,T., Rubio,I., Loubtchenkov,M.,
            Malek,D., Stoyanova,S., Vanhaesebroeck,B., Dhand,R., Nurnberg,B. et
            al.
  TITLE     Cloning and characterization of a G protein-activated human
            phosphoinositide-3 kinase
  JOURNAL   Science 269 (5224), 690-693 (1995)
   PUBMED   7624799
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK309074.1, AY725854.1,
            AB028925.1 and BC028212.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 13, 2020 this sequence version replaced NM_001142633.2.
            
            Summary: Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the
            inositol ring of phosphatidylinositol at the 3-prime position, and
            play important roles in cell growth, proliferation,
            differentiation, motility, survival and intracellular trafficking.
            The PI3Ks are divided into three classes: I, II and III, and only
            the class I PI3Ks are involved in oncogenesis. This gene encodes
            the 101 kD regulatory subunit of the class I PI3K gamma complex,
            which is a dimeric enzyme, consisting of a 110 kD catalytic subunit
            gamma and a regulatory subunit of either 55, 87 or 101 kD. This
            protein recruits the catalytic subunit from the cytosol to the
            plasma membrane through high-affinity interaction with G-beta-gamma
            proteins. Multiple alternatively spliced transcript variants
            encoding two distinct isoforms have been found. [provided by
            RefSeq, Oct 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.1770904.1,
                                           SRR14038194.1043257.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000447110.6/ ENSP00000392812.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AK309074.1         6-117
            113-1171            AY725854.1         21-1079
            1172-2768           AB028925.1         1047-2643
            2769-4491           BC028212.1         2590-4312
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..4491
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /note="phosphoinositide-3-kinase regulatory subunit 5"
                     /db_xref="GeneID:23533"
                     /db_xref="HGNC:HGNC:30035"
                     /db_xref="MIM:611317"
     exon            1..112
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            113..228
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..2768
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein FOAP-2; ptdIns-3-kinase p101; PI3-kinase p101
                     subunit; phosphatidylinositol 4,5-bisphosphate 3-kinase
                     regulatory subunit"
                     /codon_start=1
                     /product="phosphoinositide 3-kinase regulatory subunit 5
                     isoform 1"
                     /protein_id="NP_001136105.1"
                     /db_xref="CCDS:CCDS11147.1"
                     /db_xref="GeneID:23533"
                     /db_xref="HGNC:HGNC:30035"
                     /db_xref="MIM:611317"
                     /translation="MQPGATTCTEDRIQHALERCLHGLSLSRRSTSWSAGLCLNCWSL
                     QELVSRDPGHFLILLEQILQKTREVQEKGTYDLLTPLALLFYSTVLCTPHFPPDSDLL
                     LKAASTYHRFLTWPVPYCSICQELLTFIDAELKAPGISYQRLVRAEQGLPIRSHRSST
                     VTVLLLNPVEVQAEFLAVANKLSTPGHSPHSAYTTLLLHAFQATFGAHCDVPGLHCRL
                     QAKTLAELEDIFTETAEAQELASGIGDAAEARRWLRTKLQAVGEKAGFPGVLDTAKPG
                     KLHTIPIPVARCYTYSWSQDSFDILQEILLKEQELLQPGILGDDEEEEEEEEEVEEDL
                     ETDGHCAERDSLLSTSSLASHDSTLSLASSQASGPALSRHLLTSFVSGLSDGMDSGYV
                     EDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTA
                     LPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSG
                     DEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHG
                     TSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLG
                     LMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFI
                     TGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISG
                     RSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTS
                     QIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDL
                     PAQAAPDLCSLLCLPIMTFSGALP"
     exon            229..329
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            330..398
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            399..537
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            538..607
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            608..782
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            783..936
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1020
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1741
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1904
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2030
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2159
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2253
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2330
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2424
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2507
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2508..2620
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..4491
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4469..4474
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /note="hexamer: AATAAA"
     polyA_site      4491
                     /gene="PIK3R5"
                     /gene_synonym="F730038I15Rik; FOAP-2; p101; P101-PI3K"
                     /note="major polyA site"
ORIGIN      
        1 agatgctgcg gctcgccgcg cggctccagc tggggcgcgg tggtcccgac ggtcacccga
       61 atcgcaggga gtcgccgctg gagaggggct cggcgcccgg cggggactgc aggcgatgac
      121 ccaggatgca gccaggggcc acgacatgca cggaggaccg catccagcat gccctggaac
      181 gctgcctgca tggactcagc ctcagccgcc gctccacctc ctggtcagct gggctgtgtc
      241 tgaactgctg gagcctgcag gagctggtca gcagggaccc gggccacttc cttatcctcc
      301 ttgagcagat cctgcagaag acccgagagg tccaggagaa gggcacctac gacctgctca
      361 ccccgctggc cctgctcttc tattccactg ttctttgtac accacacttc ccaccagact
      421 cggatctcct tctgaaggca gccagcacct accaccggtt cctgacctgg cctgttcctt
      481 actgcagcat ctgccaggag ctgctcacct tcattgatgc tgaactcaag gccccaggga
      541 tctcctacca gagactggtg agggctgagc agggcctgcc catcaggagt caccgcagct
      601 ccaccgtcac cgtgctgctg ctgaacccag tggaagtgca ggccgagttc cttgctgtag
      661 ccaataagct gagtacgccc ggacactcgc ctcacagtgc ctacaccacc ctgctcctgc
      721 acgccttcca ggccaccttt ggggcccact gtgacgtccc gggcctgcac tgcaggctac
      781 aggccaagac cctggcagag cttgaggaca tcttcacgga gaccgcagag gcacaggagc
      841 tggcatctgg catcggggat gctgcagagg cccggcggtg gctcaggacc aagctgcagg
      901 cggtgggaga aaaagctggc ttccctgggg tgttagacac tgcaaaacca gggaagctcc
      961 acaccatccc catccctgtc gccaggtgct acacctacag ctggagccag gacagctttg
     1021 acatcctgca ggaaatcctg ctcaaggaac aggagctact ccagccaggg atcctgggag
     1081 atgatgaaga ggaggaagag gaggaggagg aggtggagga ggacttggaa actgacgggc
     1141 actgtgccga gagagattcc ctgctctcca ccagctcttt ggcgtcccat gactccacct
     1201 tgtcccttgc atcctcccag gcctcggggc cggccctctc gcgccatctg ctgacttcct
     1261 ttgtctcagg cctctctgat ggcatggaca gcggctacgt ggaggacagc gaggagagct
     1321 cctccgagtg gccttggagg cgtggcagcc aggaacgccg aggccaccgc aggcctgggc
     1381 agaagttcat caggatctat aaactcttca agagcaccag ccagctggta ctgcggaggg
     1441 actctcggag cctggagggc agctcggaca cggccctgcc cctgaggcgg gcagggagcc
     1501 tctgcagccc cctggacgaa ccagtatcac ccccttcccg ggcccagcgc tcccgctccc
     1561 tgccccagcc caaactcggt acccagctgc ccagctggct tctggcccct gcttcacgcc
     1621 cccagcgccg ccgccccttc ctgagtggag atgaggatcc caaggcttcc acgctacgtg
     1681 ttgtggtctt tggctccgat cggatttcag ggaaggtggc tcgggcgtac agcaaccttc
     1741 ggcggctgga gaacaatcgc ccactcctca cacggttctt caaacttcag ttcttctacg
     1801 tgcctgtgaa gcgaagtcat gggaccagcc ctggtgcctg tccaccccct cggagccaga
     1861 cgccctcacc cccgacagac tcccctaggc acgccagccc tggagagctg ggcaccaccc
     1921 catgggagga gagcaccaat gacatctccc actacctcgg catgctggac ccctggtatg
     1981 agcgcaatgt actgggcctc atgcacctgc cccctgaagt cctgtgccag cagtccctga
     2041 aggctgaagc ccaggccctg gagggctccc caacccagct gcccatcctg gctgacatgc
     2101 tactctacta ctgccgcttt gccgccagac cggtgctgct gcaagtctat cagaccgagc
     2161 tgaccttcat cactggggag aagacgacag agatcttcat ccactccttg gagctgggtc
     2221 actccgctgc cacacgtgcc atcaaggcgt caggtcctgg cagcaagcgg ctgggcatcg
     2281 atggcgaccg ggaggctgtt cctctaacac tacagattat ttacagcaag ggggccatca
     2341 gtggacgaag tcgctggagc aacctggaga aggtctgtac ctccgtgaac ctcaacaagg
     2401 cctgccggaa gcaggaggag ctggattcca gcatggaggc cctgacgcta aacctgacag
     2461 aagtggtgaa aaggcagaac tccaaatcca agaagggctt taaccagatt agcacatcgc
     2521 agatcaaagt ggacaaggtg cagatcatcg gctccaacag ctgccccttt gctgtgtgcc
     2581 tggaccagga tgagagaaag atcctgcaga gtgtagtcag atgtgaggtc tcaccgtgct
     2641 acaagccaga gaagagcgac ctctcctcac caccccagac gcctcctgac ctgccggccc
     2701 aggccgcacc tgatctctgc tcccttctct gcctgcccat catgactttc agtggagctc
     2761 tgccctagtg tgggcccagc gccagactgg acagaagccc tggggcaacc tcctcggcca
     2821 cccctccagg acagtccctc tctgtggaga actgaatggc cctgtgcaga gccatagtcc
     2881 cactgtgggt cctgcaatga gcaggggctg ggagtagagg gtttctgggg cctcagggtt
     2941 ctgggaaagc aacagctatc agagagagaa gggccagacc ccatagcctc ttagattcct
     3001 ggcagtagaa ggagaaggat gggtaaattg acctctgaag tccctgacca ttagcatggt
     3061 ctaggatcct ttctagaagg aagatctgag gctctggtgc tcagggggat ggcttgggcc
     3121 ttttctctca accttggctg agcctacccc ttactttgcc aaagacttga ggaccctgta
     3181 tgtctggagt tcagtcccct cctctgtggg gctcaggtga ttgaaatgtg gatgaaacat
     3241 ttctctactt caagaccacc tctccctgca aacaccacac acacatggca tgcatgtacg
     3301 cacatgcgca cacacacacg cacacacctc aataatttct ctcaagtttc ctgagtctcc
     3361 agaaaaacag cactaacgct ggacctgtct actctcagaa cccggcacag attctctctt
     3421 gatctccttt tggaatctga gattcttaga agacaggata gggttaaatt tagtagcagc
     3481 tcagttctag ctaaatcact agaggaagtt aattaacttt aagccttcat ttctccagca
     3541 ctaaaatgga gtggagagtt ggggtggaaa taagacatcc ttaaaaggtt aaattgtctg
     3601 caaagcacct agcccagtgc cgagctccca gtaggtgttc agtaaagctt agtgcctgac
     3661 tttctgaaca ctgattcctc ctgtttggag tcactgggat actctcattg ccgttgggat
     3721 gttcctcact ccttcccagt tcgtggctga ggcagaaccc agactgaaga gggaagagac
     3781 attccagagg aggattgcct tcgtcagggt aaggggtggg ctgctcaggg gccctaccct
     3841 tcaccccctt ctgtatcaga ttggccctcc cactcccatc tcactctgcg tgtacaatct
     3901 tccatatccg caagttcact ggcactcttc tggcacctgg gcaagatccc agaacagagg
     3961 atggagtgac tggcctcaca gagcttagtg cccgactcag gggaaatggg actggtgcat
     4021 gggaaatggt cagcctagga taggacacga gagtctgaaa ttcaaagcaa ccagcttgaa
     4081 gtggtttgag aagctggaag caaacatggg ctagagagat agggcagaag tcaagacgag
     4141 gatctggact gatgtggaga aagtagccac ggaagcatga actgtatcct gcacaaagtc
     4201 cctcttcccc gcctcctaat tcattatgcc caaaaggcct tacgtgaaat tccagcccag
     4261 agtactcatg acttgagaga cgtggacaga gccagcttct accttgcctg gccgtctctc
     4321 ccctgtctta atgtctgctc ttgctctaag ctccagaaga gtggcgggcc atgtatcttc
     4381 aatatgtttt tgctgtatgg gcaggttgtc ttattatgtg atcaacagat gtccaggaac
     4441 taatgagtgg aatttaatat tattgtcaaa taaaacttga tttgtcctat a
//
