LOCUS       NM_014602               5016 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens phosphoinositide-3-kinase regulatory subunit 4
            (PIK3R4), mRNA.
ACCESSION   NM_014602
VERSION     NM_014602.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Mercer,T.J., Ohashi,Y., Boeing,S., Jefferies,H.B.J., De Tito,S.,
            Flynn,H., Tremel,S., Zhang,W., Wirth,M., Frith,D., Snijders,A.P.,
            Williams,R.L. and Tooze,S.A.
  TITLE     Phosphoproteomic identification of ULK substrates reveals
            VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy
  JOURNAL   EMBO J 40 (14), e105985 (2021)
   PUBMED   34121209
  REMARK    GeneRIF: Phosphoproteomic identification of ULK substrates reveals
            VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy.
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5016)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   4  (bases 1 to 5016)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5016)
  AUTHORS   Gstrein,T., Edwards,A., Pristoupilova,A., Leca,I., Breuss,M.,
            Pilat-Carotta,S., Hansen,A.H., Tripathy,R., Traunbauer,A.K.,
            Hochstoeger,T., Rosoklija,G., Repic,M., Landler,L., Stranecky,V.,
            Durnberger,G., Keane,T.M., Zuber,J., Adams,D.J., Flint,J.,
            Honzik,T., Gut,M., Beltran,S., Mechtler,K., Sherr,E., Kmoch,S.,
            Gut,I. and Keays,D.A.
  TITLE     Mutations in Vps15 perturb neuronal migration in mice and are
            associated with neurodevelopmental disease in humans
  JOURNAL   Nat Neurosci 21 (2), 207-217 (2018)
   PUBMED   29311744
  REMARK    GeneRIF: The mutations in VPS15 are associated with cortical
            atrophy and epilepsy in humans.
            Erratum:[Nat Neurosci. 2018 Aug;21(8):1139. doi:
            10.1038/s41593-018-0170-9.. PMID: 29875394]
REFERENCE   6  (bases 1 to 5016)
  AUTHORS   Ligon,L.A., Shelly,S.S., Tokito,M. and Holzbaur,E.L.
  TITLE     The microtubule plus-end proteins EB1 and dynactin have
            differential effects on microtubule polymerization
  JOURNAL   Mol Biol Cell 14 (4), 1405-1417 (2003)
   PUBMED   12686597
REFERENCE   7  (bases 1 to 5016)
  AUTHORS   Koyasu,S.
  TITLE     The role of PI3K in immune cells
  JOURNAL   Nat Immunol 4 (4), 313-319 (2003)
   PUBMED   12660731
  REMARK    Review article
REFERENCE   8  (bases 1 to 5016)
  AUTHORS   Askham,J.M., Vaughan,K.T., Goodson,H.V. and Morrison,E.E.
  TITLE     Evidence that an interaction between EB1 and p150(Glued) is
            required for the formation and maintenance of a radial microtubule
            array anchored at the centrosome
  JOURNAL   Mol Biol Cell 13 (10), 3627-3645 (2002)
   PUBMED   12388762
REFERENCE   9  (bases 1 to 5016)
  AUTHORS   Petiot,A., Ogier-Denis,E., Blommaart,E.F., Meijer,A.J. and
            Codogno,P.
  TITLE     Distinct classes of phosphatidylinositol 3'-kinases are involved in
            signaling pathways that control macroautophagy in HT-29 cells
  JOURNAL   J Biol Chem 275 (2), 992-998 (2000)
   PUBMED   10625637
  REMARK    Erratum:[J Biol Chem 2000 Apr 21;275(16):12360]
REFERENCE   10 (bases 1 to 5016)
  AUTHORS   Panaretou,C., Domin,J., Cockcroft,S. and Waterfield,M.D.
  TITLE     Characterization of p150, an adaptor protein for the human
            phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by
            phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase
            complex
  JOURNAL   J Biol Chem 272 (4), 2477-2485 (1997)
   PUBMED   8999962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP359657.1 and Y08991.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014602.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC110318.1, Y08991.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356763.8/ ENSP00000349205.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BP359657.1         24-46
            24-5016             Y08991.1           33-5025
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.1"
     gene            1..5016
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /note="phosphoinositide-3-kinase regulatory subunit 4"
                     /db_xref="GeneID:30849"
                     /db_xref="HGNC:HGNC:8982"
                     /db_xref="MIM:602610"
     exon            1..512
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            513..1291
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     CDS             559..4635
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /EC_number="2.7.11.1"
                     /note="phosphatidylinositol 3-kinase-associated p150;
                     phosphoinositide-3-kinase, regulatory subunit 4, p150;
                     PI3-kinase regulatory subunit 4; PI3-kinase p150 subunit;
                     phosphoinositide 3-kinase adaptor protein"
                     /codon_start=1
                     /product="phosphoinositide 3-kinase regulatory subunit 4"
                     /protein_id="NP_055417.1"
                     /db_xref="CCDS:CCDS3067.1"
                     /db_xref="GeneID:30849"
                     /db_xref="HGNC:HGNC:8982"
                     /db_xref="MIM:602610"
                     /translation="MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAK
                     HREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLF
                     RQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWN
                     WVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDP
                     STPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQ
                     VLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKE
                     TFLSADERILVIRKDLGNIIHNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYC
                     DSKLAALELILHLAPRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKE
                     VPRNDINIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLNME
                     NDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCV
                     FFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGL
                     SDAEEFVIVKALYALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVV
                     ARQISTADVYCKLMPYLDPYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITS
                     LFRHLHMRQKKRNGSLPDCPPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSN
                     KAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVKQDSNVN
                     EEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSSQVPEVTTV
                     QNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWES
                     KPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIWNSQKMEGKTT
                     TTRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSR
                     ILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITS
                     FAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAV
                     QGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAGSD
                     MKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRG
                     PESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK"
     exon            1292..1425
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..2008
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2143
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2365
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            2366..2539
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2685
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2889
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..3091
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            3092..3279
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            3280..3490
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..3656
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            3657..3821
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..4033
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            4034..4165
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            4166..4266
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            4267..4355
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4464
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     exon            4465..5016
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4984..4989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /note="hexamer: ATTAAA"
     polyA_site      5016
                     /gene="PIK3R4"
                     /gene_synonym="p150; VPS15"
                     /note="major polyA site"
ORIGIN      
        1 gaggtcagac cggttgcttt cccgggagtt cggcgtttgc tggggctgca gcagctgaag
       61 tgtagtgttt tcttgggact ggcggtctgc acttctctcc cgggttccat ctccccccgc
      121 ccggtggtga ggccctcgag gagggctcgg acgggtgtag cgatccgcgc tagaggaaga
      181 cgaggcccgg gaacgcatgt cccccagggc aggttagggg gctggagggg tcaaatcccg
      241 gggtacttgt ggagactctt tagcgtggct tcttctctct gctgagaccc cgagagcttt
      301 cccagttctc ctcccaggac caccggggtt cctgaagatc gggacttttc tgcgcccctc
      361 caccaacagc ccatctcctg tctatgaaga aagacccttc gtagaaacaa cttccccgct
      421 gctgacgcgt tttcccgtcc cgtccccgaa gtagtctact atgacctcgt tgtgagcctc
      481 tgaacgattt tgacactttc ccgaggccta gggtattata tcctaacctt actaaagacc
      541 acagaggtgc ttgccattat gggaaatcag cttgctggca ttgctccctc ccagatcctt
      601 tctgtagaga gttatttttc agatattcat gactttgaat atgataaaag cctggggagt
      661 actcggtttt ttaaagttgc tcgagccaag caccgagaag gcctggtcgt tgtgaaggtt
      721 tttgcaattc aggatcccac attgccttta accagctata aacaagagct ggaggaactg
      781 aaaatcaggc ttaattctgc acagaattgt ctacctttcc agaaagcatc agaaaaagca
      841 tctgagaaag cagctatgct ctttaggcag tatgtgcgag acaatctcta tgatcgcatc
      901 agtacccgtc cattcttgaa taacattgag aagcgctgga ttgctttcca gatcctgaca
      961 gctgtggacc aagcacacaa atctggagtt cgtcatgggg acatcaagac tgagaatgtg
     1021 atggtcacca gttggaattg ggttcttcta actgattttg ccagttttaa gcccacttat
     1081 cttccagaag acaacccggc agatttcaat tatttctttg acacatcacg gaggagaact
     1141 tgctatattg ctcctgaacg ttttgttgat ggtgggatgt ttgccactga gttagaatat
     1201 atgagagatc cttcaactcc gcttgtagac ttaaatagca atcagagaac aagaggagag
     1261 ttgaagagag caatggacat cttttcagca ggttgtgtga tagctgagct ttttacagaa
     1321 ggtgtaccat tatttgatct ctctcaactt ttggcttata gaaatggaca ttttttccct
     1381 gaacaagtgc taaataaaat tgaagatcac agtatcagag aattggtaac tcagatgatt
     1441 caccgtgagc cagataaacg tttagaggca gaagattact taaaacagca gcgtggcaat
     1501 gcctttcctg aaatatttta cacttttctt cagccctaca tggcccagtt tgccaaggaa
     1561 acgtttcttt ctgcagatga gcgtattctg gttatacgga aggatttggg caacattatt
     1621 cacaatctct gtggacatga tctgccagaa aaagccgaag gagagcctaa ggaaaatggg
     1681 ctggttatct tggtatctgt tataacatcc tgcctacaga cccttaaata ctgtgattcc
     1741 aaactagctg ctttggaact gattcttcat ttggctccaa gattaagtgt tgaaatcctt
     1801 ttggatcgta ttactccata tcttttgcat ttcagcaatg actctgttcc tagggtgagg
     1861 gctgaagcct tgaggacgtt gaccaaagtt cttgctctcg tcaaagaggt tcctcgtaat
     1921 gatatcaata tttatccgga atacattctg ccaggcatag cccacttagc ccaagatgat
     1981 gctactatcg ttagactagc ctatgctgaa aacatagctc tgctggcaga aacagctctg
     2041 agattcctgg aattagtaca gttaaaaaat cttaatatgg aaaatgaccc caataatgaa
     2101 gaaatagatg aggttacaca tccaaatgga aattatgaca cagagctcca agccttacat
     2161 gaaatggtcc agcagaaagt tgttactttg ctaagtgacc ctgaaaatat tgtaaaacaa
     2221 accttgatgg aaaatggaat aacacggctg tgtgtattct ttggacgtca gaaagccaac
     2281 gatgttttgt tgtcccacat gattactttc ctaaatgata agaatgattg gcatctacgt
     2341 ggagcatttt ttgatagtat agttggtgtt gctgcctatg ttggctggca aagctcctca
     2401 attctcaagc ctctgctgca acaaggtctt agtgatgctg aggaatttgt cattgtgaaa
     2461 gctctttatg cccttacttg tatgtgccag ttaggactgc tacaaaaacc ccatgtttac
     2521 gaatttgcca gtgatattgc ccccttcctg tgtcatccca atttatggat acgttatggt
     2581 gccgtgggat ttatcacagt ggtagctcgt caaataagta cagctgatgt ctactgtaaa
     2641 ctgatgcctt atcttgaccc atatattacc caaccaataa tacagattga aagaaaactt
     2701 gttctgctca gtgttttaaa ggaaccagta agtcgttcta tatttgatta tgctttgagg
     2761 tctaaagata ttactagctt gttcagacat cttcacatgc gtcagaagaa acgaaatggt
     2821 tctcttcccg actgccctcc gccagaggat cctgccatag cacagcttct gaagaagttg
     2881 ctctcacagg gaatgacaga ggaagaggaa gacaaacttc tggcactgaa agacttcatg
     2941 atgaaatcta ataaagcaaa ggccaatata gtggaccaga gccatcttca tgatagtagt
     3001 cagaaaggtg taattgactt ggcagcttta ggcataactg ggagacaagt tgatcttgtt
     3061 aaaaccaaac aagaaccaga tgacaaacgg gccagaaaac atgtaaaaca agactcaaat
     3121 gtaaatgaag aatggaaaag catgtttggg tcactggacc caccaaacat gccacaggcc
     3181 ctacctaaag ggagtgatca ggaggtgatt cagactggga aacctcctcg ttccgagtcc
     3241 tctgctggca tttgtgtccc tttgtcaact tcttcacagg ttccagaagt gacaactgtc
     3301 caaaataaaa aaccagtaat accggtttta agtagtacaa tcttaccatc cacctatcag
     3361 attcgaatta caacttgtaa aactgaactt cagcaactca tccagcaaaa gcgggagcag
     3421 tgcaatgctg agagaatagc taagcagatg atggaaaatg ctgaatggga gagtaaacca
     3481 ccaccacctg gatggcgtcc taaagggctg ttagttgccc atcttcatga gcataaatct
     3541 gctgtgaatc gaattagagt ctctgatgaa cactcacttt ttgcaacatg ttcaaatgat
     3601 ggcacagtga aaatctggaa cagtcaaaag atggagggga agaccaccac taccagatct
     3661 attcttacat acagccgaat tggaggacga gtcaagacgc tcacattctg ccaaggctcc
     3721 cactatttag ccatagcatc tgataatggt gctgtccagc ttcttggaat tgaggcttct
     3781 aagctgccca agtctcctaa aatccatcct ctacaaagca gaattctaga tcagaaggag
     3841 gacggttgtg ttgtggatat gcatcacttc aactctggag cacagtctgt tcttgcctat
     3901 gccactgtga atggctctct ggttggctgg gaccttaggt cttcaagcaa tgcgtggact
     3961 ttaaagcatg atttaaagtc gggcctcatc acttcctttg ctgtggacat ccaccaatgc
     4021 tggctctgca ttggtacaag cagtggtacc atggcttgtt gggacatgag gttccagttg
     4081 ccaatttcaa gtcactgtca tccttccagg gctcgaatca gacgcctctc aatgcaccct
     4141 ctgtatcagt cctgggtgat tgcagctgtt cagggcaaca acgaagtgtc catgtgggac
     4201 atggagactg gtgacagaag atttactctc tgggccagca gtgcaccacc actttctgaa
     4261 ttacagcctt ctcctcatag cgtccatggt atctactgta gtcctgcaga tggaaatcct
     4321 atcctactaa cagctggctc agatatgaaa ataaggtttt gggacttggc ttacccagaa
     4381 aggtcctatg ttgttgcagg aagtactagt tccccatctg tgtcctacta caggaaaata
     4441 attgaaggca ctgaagttgt ccaggaaatt cagaataagc agaaagtagg accaagtgat
     4501 gacacccctc gaaggggccc agagtccctg cccgtgggac atcatgacat catcactgat
     4561 gtcgccacat tccagaccac acagggcttc atcgtaactg cttctagaga tgggattgtg
     4621 aaggtgtgga aataaaacct actgatttgt ataaatttta atagttataa atataatact
     4681 ataactcgag aaaaggcatt tctagagaac agattcattt gcttaatttt caaaattatg
     4741 tctccatatt actgtttcat gactgactga ctaaatgaca cccaaaatgg ttaagatgta
     4801 cttgactagt ttacttatgc atctctttgc aagaatcagc cagccaacaa tgtctgggat
     4861 ttttattgta tatgttatag aggtgagaaa tgtaaaatat gaaaatgaat atgtttattt
     4921 tgtattgaaa aagatggttg aaaagatggt tgtaagctat tatagtataa acacattttt
     4981 gctattaaaa atgctattca aagcagttaa actgta
//
