LOCUS       NM_181523               6975 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens phosphoinositide-3-kinase regulatory subunit 1
            (PIK3R1), transcript variant 1, mRNA.
ACCESSION   NM_181523
VERSION     NM_181523.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Lyu,P., Li,F., Deng,R., Wei,Q., Lin,B., Cheng,L., Zhao,B. and Lu,Z.
  TITLE     Lnc-PIK3R1, transcriptionally suppressed by YY1, inhibits
            hepatocellular carcinoma progression via the
            Lnc-PIK3R1/miR-1286/GSK3beta axis
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (6), 167233 (2024)
   PUBMED   38744342
  REMARK    GeneRIF: Lnc-PIK3R1, transcriptionally suppressed by YY1, inhibits
            hepatocellular carcinoma progression via the
            Lnc-PIK3R1/miR-1286/GSK3beta axis.
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Thapa,N., Chen,M., Cryns,V.L. and Anderson,R.
  TITLE     A p85 isoform switch enhances PI3K activation on endosomes by a
            MAP4- and PI3P-dependent mechanism
  JOURNAL   Cell Rep 43 (5), 114119 (2024)
   PUBMED   38630589
  REMARK    GeneRIF: A p85 isoform switch enhances PI3K activation on endosomes
            by a MAP4- and PI3P-dependent mechanism.
REFERENCE   3  (bases 1 to 6975)
  AUTHORS   Cobleigh,M.A., Layng,K.V., Mauer,E., Mahon,B., Hockenberry,A.J. and
            Abukhdeir,A.M.
  TITLE     Comparative genomic analysis of PIK3R1-mutated and wild-type breast
            cancers
  JOURNAL   Breast Cancer Res Treat 204 (2), 407-414 (2024)
   PUBMED   38153569
  REMARK    GeneRIF: Comparative genomic analysis of PIK3R1-mutated and
            wild-type breast cancers.
REFERENCE   4  (bases 1 to 6975)
  AUTHORS   Liu,J., Lin,F., Sun,Y. and Liu,X.
  TITLE     Clinicopathological analysis of rosette-forming glioneuronal tumors
  JOURNAL   Diagn Pathol 19 (1), 39 (2024)
   PUBMED   38388383
  REMARK    GeneRIF: Clinicopathological analysis of rosette-forming
            glioneuronal tumors.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6975)
  AUTHORS   Sacco,K. and Uzel,G.
  TITLE     Activated PI3K Delta Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   39899769
REFERENCE   6  (bases 1 to 6975)
  AUTHORS   Innes,A.M. and Dyment,D.A.
  TITLE     SHORT Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24830046
REFERENCE   7  (bases 1 to 6975)
  AUTHORS   Panayotou,G., Bax,B., Gout,I., Federwisch,M., Wroblowski,B.,
            Dhand,R., Fry,M.J., Blundell,T.L., Wollmer,A. and Waterfield,M.D.
  TITLE     Interaction of the p85 subunit of PI 3-kinase and its N-terminal
            SH2 domain with a PDGF receptor phosphorylation site: structural
            features and analysis of conformational changes
  JOURNAL   EMBO J 11 (12), 4261-4272 (1992)
   PUBMED   1330535
REFERENCE   8  (bases 1 to 6975)
  AUTHORS   Hiles,I.D., Otsu,M., Volinia,S., Fry,M.J., Gout,I., Dhand,R.,
            Panayotou,G., Ruiz-Larrea,F., Thompson,A., Totty,N.F. et al.
  TITLE     Phosphatidylinositol 3-kinase: structure and expression of the 110
            kd catalytic subunit
  JOURNAL   Cell 70 (3), 419-429 (1992)
   PUBMED   1322797
REFERENCE   9  (bases 1 to 6975)
  AUTHORS   Volinia,S., Patracchini,P., Otsu,M., Hiles,I., Gout,I.,
            Calzolari,E., Bernardi,F., Rooke,L. and Waterfield,M.D.
  TITLE     Chromosomal localization of human p85 alpha, a subunit of
            phosphatidylinositol 3-kinase, and its homologue p85 beta
  JOURNAL   Oncogene 7 (4), 789-793 (1992)
   PUBMED   1314371
REFERENCE   10 (bases 1 to 6975)
  AUTHORS   Kashishian,A., Kazlauskas,A. and Cooper,J.A.
  TITLE     Phosphorylation sites in the PDGF receptor with different
            specificities for binding GAP and PI3 kinase in vivo
  JOURNAL   EMBO J 11 (4), 1373-1382 (1992)
   PUBMED   1314164
  REMARK    Erratum:[EMBO J 1992 Oct;11(10):3809]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CT003423.1, BC094795.1,
            AC016564.6 and CA427864.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_181523.2.
            
            Summary: Phosphatidylinositol 3-kinase phosphorylates the inositol
            ring of phosphatidylinositol at the 3-prime position. The enzyme
            comprises a 110 kD catalytic subunit and a regulatory subunit of
            either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory
            subunit. Phosphatidylinositol 3-kinase plays an important role in
            the metabolic actions of insulin, and a mutation in this gene has
            been associated with insulin resistance. Alternative splicing of
            this gene results in four transcript variants encoding different
            isoforms. [provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1) of this protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.38124.1,
                                           SRR1803614.221726.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000521381.6/ ENSP00000428056.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                CT003423.1         1-20
            21-4390             BC094795.1         1-4370
            4391-6606           AC016564.6         26540-28755         c
            6607-6975           CA427864.1         17-385              c
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.1"
     gene            1..6975
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /note="phosphoinositide-3-kinase regulatory subunit 1"
                     /db_xref="GeneID:5295"
                     /db_xref="HGNC:HGNC:8979"
                     /db_xref="MIM:171833"
     exon            1..194
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            195..914
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             581..2755
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphatidylinositol 3-kinase, regulatory subunit,
                     polypeptide 1 (p85 alpha); growth factor receptor bound 1;
                     PI3 kinase-associated p85; phosphatidylinositol
                     3-kinase-associated p-85 alpha; PI3-kinase subunit
                     p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase
                     regulatory subunit alpha; phosphatidylinositol 3-kinase 85
                     kDa regulatory subunit alpha; phosphoinositide-3-kinase,
                     regulatory subunit 1 (alpha); phosphoinositide-3-kinase
                     regulatory subunit alpha"
                     /codon_start=1
                     /product="phosphatidylinositol 3-kinase regulatory subunit
                     alpha isoform 1"
                     /protein_id="NP_852664.1"
                     /db_xref="CCDS:CCDS3993.1"
                     /db_xref="GeneID:5295"
                     /db_xref="HGNC:HGNC:8979"
                     /db_xref="MIM:171833"
                     /translation="MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSD
                     GQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGSS
                     KTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAE
                     LRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEE
                     YIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFS
                     AASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWY
                     WGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGF
                     SDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLH
                     EYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEY
                     IEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSI
                     KPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTW
                     NVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFA
                     EPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR"
     exon            915..1007
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1082
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1214
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1416
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1496
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..1599
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..1698
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1879
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2005
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2006..2148
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2149..2325
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2394
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2565
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..6975
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6952..6957
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /note="hexamer: AATAAA"
     polyA_site      6975
                     /gene="PIK3R1"
                     /gene_synonym="AGM7; GRB1; IMD36; p85; p85-ALPHA;
                     p85alpha"
                     /note="major polyA site"
ORIGIN      
        1 gtcgccagca gctggagcgg agttggagga agcagcggca gcggcgaggg cggcaggcta
       61 gctgtcggag acggcaagca cggatcgggc acggccggcg ggtcgcagcc gggctggagg
      121 ccggtcggaa ccgagcgggc aggaggccac cgctgcagcg cgggccccgc agaggaagga
      181 agccggcggg cggggtgaag ctcgtgtgtg gagtgccacg gtacaatcag acgacagatg
      241 gacagtgtga caaaagtgtc agaaaggatt gggcctcgct gtgagagtca gcctggattc
      301 aaagtgttga caagttgctg aaaaggaagc cagtgagagg actgtggcac gcagaggaag
      361 tggagccctg tcttcggtca caccattgat ggaggacaga tggacagccg tatggccagt
      421 cacctctcct cttaaacctt tggagagtgg tcctttgtcc tctgctggac acataatagg
      481 aattctaaca cattctctga attcactttt cataaaaacg taaaatcaga ctgctctgta
      541 caaccaggct caactgttgc atggtagcag atttgcaaac atgagtgctg aggggtacca
      601 gtacagagcg ctgtatgatt ataaaaagga aagagaagaa gatattgact tgcacttggg
      661 tgacatattg actgtgaata aagggtcctt agtagctctt ggattcagtg atggacagga
      721 agccaggcct gaagaaattg gctggttaaa tggctataat gaaaccacag gggaaagggg
      781 ggactttccg ggaacttacg tagaatatat tggaaggaaa aaaatctcgc ctcccacacc
      841 aaagccccgg ccacctcggc ctcttcctgt tgcaccaggt tcttcgaaaa ctgaagcaga
      901 tgttgaacaa caagctttga ctctcccgga tcttgcagag cagtttgccc ctcctgacat
      961 tgccccgcct cttcttatca agctcgtgga agccattgaa aagaaaggtc tggaatgttc
     1021 aactctatac agaacacaga gctccagcaa cctggcagaa ttacgacagc ttcttgattg
     1081 tgatacaccc tccgtggact tggaaatgat cgatgtgcac gttttggctg acgctttcaa
     1141 acgctatctc ctggacttac caaatcctgt cattccagca gccgtttaca gtgaaatgat
     1201 ttctttagct ccagaagtac aaagctccga agaatatatt cagctattga agaagcttat
     1261 taggtcgcct agcatacctc atcagtattg gcttacgctt cagtatttgt taaaacattt
     1321 cttcaagctc tctcaaacct ccagcaaaaa tctgttgaat gcaagagtac tctctgaaat
     1381 tttcagccct atgcttttca gattctcagc agccagctct gataatactg aaaacctcat
     1441 aaaagttata gaaattttaa tctcaactga atggaatgaa cgacagcctg caccagcact
     1501 gcctcctaaa ccaccaaaac ctactactgt agccaacaac ggtatgaata acaatatgtc
     1561 cttacaagat gctgaatggt actggggaga tatctcgagg gaagaagtga atgaaaaact
     1621 tcgagataca gcagacggga cctttttggt acgagatgcg tctactaaaa tgcatggtga
     1681 ttatactctt acactaagga aagggggaaa taacaaatta atcaaaatat ttcatcgaga
     1741 tgggaaatat ggcttctctg acccattaac cttcagttct gtggttgaat taataaacca
     1801 ctaccggaat gaatctctag ctcagtataa tcccaaattg gatgtgaaat tactttatcc
     1861 agtatccaaa taccaacagg atcaagttgt caaagaagat aatattgaag ctgtagggaa
     1921 aaaattacat gaatataaca ctcagtttca agaaaaaagt cgagaatatg atagattata
     1981 tgaagaatat acccgcacat cccaggaaat ccaaatgaaa aggacagcta ttgaagcatt
     2041 taatgaaacc ataaaaatat ttgaagaaca gtgccagacc caagagcggt acagcaaaga
     2101 atacatagaa aagtttaaac gtgaaggcaa tgagaaagaa atacaaagga ttatgcataa
     2161 ttatgataag ttgaagtctc gaatcagtga aattattgac agtagaagaa gattggaaga
     2221 agacttgaag aagcaggcag ctgagtatcg agaaattgac aaacgtatga acagcattaa
     2281 accagacctt atccagctga gaaagacgag agaccaatac ttgatgtggt tgactcaaaa
     2341 aggtgttcgg caaaagaagt tgaacgagtg gttgggcaat gaaaacactg aagaccaata
     2401 ttcactggtg gaagatgatg aagatttgcc ccatcatgat gagaagacat ggaatgttgg
     2461 aagcagcaac cgaaacaaag ctgaaaacct gttgcgaggg aagcgagatg gcacttttct
     2521 tgtccgggag agcagtaaac agggctgcta tgcctgctct gtagtggtgg acggcgaagt
     2581 aaagcattgt gtcataaaca aaacagcaac tggctatggc tttgccgagc cctataactt
     2641 gtacagctct ctgaaagaac tggtgctaca ttaccaacac acctcccttg tgcagcacaa
     2701 cgactccctc aatgtcacac tagcctaccc agtatatgca cagcagaggc gatgaagcgc
     2761 ttactctttg atccttctcc tgaagttcag ccaccctgag gcctctggaa agcaaagggc
     2821 tcctctccag tctgatctgt gaattgagct gcagaaacga agccatcttt ctttggatgg
     2881 gactagagct ttctttcaca aaaaagaagt aggggaagac atgcagccta aggctgtatg
     2941 atgaccacac gttcctaagc tggagtgctt atcccttctt tttctttttt tctttggttt
     3001 aatttaaagc cacaaccaca tacaacacaa agagaaaaag aaatgcaaaa atctctgcgt
     3061 gcagggacaa agaggccttt aaccatggtg cttgttaatg ctttctgaag ctttaccagc
     3121 tgaaagttgg gactctggag agcggaggag agagaggcag aagaaccctg gcctgagaag
     3181 gtttggtcca gcctggttta gcctggatgt tgctgtgcac ggtggaccca gacacatcgc
     3241 actgtggatt atttcatttt gtaacaaatg aacgatatgt agcagaaagg cacgtccact
     3301 cacaagggac gctttgggag aatgtcagtt catgtatgtt cagaagaaat tctgtcatag
     3361 aaagtgccag aaagtgttta acttgtcaaa aaacaaaaac ccagcaacag aaaaatggag
     3421 tttggaaaac aggacttaaa atgacattca gtatataaaa tatgtacata atattggatg
     3481 actaactatc aaatagatgg atttgtatca ataccaaata gcttctgttt tgttttgctg
     3541 aaggctaaat tcacagcgct atgcaattct taattttcat taagttgtta tttcagtttt
     3601 aaatgtacct tcagaataag cttccccacc ccagtttttg ttgcttgaaa atattgttgt
     3661 cccggatttt tgttaatatt catttttgtt atcctttttt aaaagtaaat gtacaggatg
     3721 ccagtaaaaa aaaaaaatgg cttcagaatt aaaactatga aatattttac agtttttctt
     3781 gtacagagta cttggctgtt agcccaaggt taaaaagttc ataacagatt ttttttggac
     3841 tgttttgttg ggcagtgcct gataagcttc aaagctgctt tattcaataa aaaaaagaaa
     3901 tgaaaaagat atatgaatat gacaaagtat tgctgagtcc aacaatgttg ttttaagact
     3961 cttaaaatac ggtacctggc aatgtttatt tcataaagaa ttgtgaactt cttgaatcta
     4021 gggaggggga atgtagtgaa gggatgtatc aagtggggtg gtgggagggg gaggcaaggt
     4081 tatatgcact ttctcatgat ttacagagaa gtgaataact gcaaagtgaa gttgcttctt
     4141 ctacttcagt cttctctcac tttgatttgc tagttgttat caattaatga caattacaaa
     4201 cctactgtat ctctaataca gtgtgactgg tcaggtattt cagttcttag gaaggaagtg
     4261 ccaagtttgt ttttgggttc ctggaacagc gctcaccttt gtttagaaca ctggtttaaa
     4321 gggataatca tctctgtcac attagactat ccatcatgac cagcaaatac tcattttagg
     4381 aaaaaaaaaa gcatgatctg aaaaatactt ttggtggtat gttggttacc ctcctagctt
     4441 tccatttggt ttagaacata aagcaaatag acacagtcat actgtcactg ctctggactg
     4501 tgtggagctc gctaaagtca tggtcattgc aggaatccaa gtggcagtcc ttctcattca
     4561 ttctaatcat tgtatgtgct tcactacggg ggggagaagg aaacgttagc atcatgtttc
     4621 ccatttaggg caggagtgag aggtctctct tcctgattta gatatgcaaa agctggtatg
     4681 ttcagtagga actgtacatg tgttgggagg cataaagact aattagcaac cataatatgg
     4741 tcactaccct aatagactaa atgaaatctt gcaatttcaa attactcttt ctccatatta
     4801 gatttaccca cagctatatt tctgtttaag tactagggtg agggttttct gttactttgt
     4861 tttttaatgt tgttcctttt gaaagaatca gtcttgcagc tgagtgaaaa atctgtggaa
     4921 tgtattattt gtcctcttta catgaaacta ctcatactta agcaaaagtc agtcttatag
     4981 caagactgtt agccctcaaa cttgactcta ctgatctgac catttccctc tcatcgccag
     5041 acaactgacg atttccctgg ttttagtctg cgtctctgct ttaaagttat tgtgatatcc
     5101 ttctagatca tacacaagtc taacagttaa ttagttaaca gtttttaaac taggtttgtg
     5161 ggtatttttt tggtagcaca tgtatgctat tacatacaaa tttttatttc taaaatataa
     5221 gatctgagat tgaatatttt cattaaaagc tacagttttg tgaatctttg tgcttcaaca
     5281 ttctttgcaa gatgatacgg tatttaggca tttgccttat ttttgcatct cacaaacata
     5341 agtgcaatag atcttttcat tgaacagcaa agtaggattc atcattccat atgacttgag
     5401 ttacaccaga cctgttctgc ccaatgcctt tttgattaca gtgtagcttg cccaccgcat
     5461 ttgtcgtttt agatactttg ctagccggcc actttggatt tcatcagaca gtcctaacaa
     5521 tattgtctga acggctgaat atgaatagat acagcagagg cactcctgat atatgatttt
     5581 tatccatgcg tcagtttttc ccacccagtg tagcatccta aagataaagc cagaagctaa
     5641 gctgcagtga ggctgtgatt gggcgtagaa gtgggagcat tgggacctca cattacacac
     5701 acgagagatc ataaccatgt gaaaaggcaa aaagcatgtg tttgcaacat ctgataactt
     5761 catggccttt gataaatgta tatatgtata tgtgcatgga ctgtgtttcc agtacacctt
     5821 tcagccaaaa cagatccaca gtagttgttg agttcaagta cataaagtac ataacaagcg
     5881 aacgtctagt acaattctta cttatgtgta tgggattttt ccctttgagg ttgctttgtt
     5941 ttgtcttaca aaggtgaaaa ttgtttgtaa gtgaagtgag aagttcatat ttctttggct
     6001 tttttgtgtt tttaaaagtt actcctttta gggagctggt ctgatgactt gcttagcttg
     6061 gaaatccttg ttttcagtgt gtcgagtcaa aatgtgttta tgtgagctgt cactgtgggg
     6121 aaccaattgc tttgtcatat agctggttat gaactagtaa catgtttggg aagtcctact
     6181 gatgttcctt tggaagaaaa aatctgctgg ttttaacaac tgtgcttttg ctatgtatgg
     6241 tatccaagtt agttgaaacg cagacactga gatctgtttg agtttagggt catttttaga
     6301 aaggggcagt ttaaagcaca atgtctcaca tgggacaaag ttccaaaatg ccaaattctt
     6361 attttttaaa aagctagttc tataaaatac tggtattatg ggtggggagg aaatagaatt
     6421 gagtcaattg gaaagactat ccaacttaac atgaaacttg tcaccatgag atagcattag
     6481 ctgcccagga tgctgctata tatatatata tatatatata tgtgtgtgtg tgtgtgtgtg
     6541 tgtgtgtata tatatatata tatatatata tatatatata tatatatgtg tgtgtatata
     6601 tatatatatg tgtatatata tatgtatata catatatgta tatatatgca catatatata
     6661 tgtatttaaa aaaatcaaaa caaaaaaaaa ctcatttata cctgtgtatt ttttaaagct
     6721 acaatctgtt caatgttttt aaaaatctgt ttatatgaca ttgttaaaat aaagttggtc
     6781 ttttgacgag agggaggatg tcacggtcag ttgtaacttt gccttcacaa ggcaactggg
     6841 gtggggggtg ggggtagtgt gcctccttga catttcgttc aagttataga ttcaatggag
     6901 ctatgtcttg ttttaagttg ctttaatgca ttgtattaga tcttcaaaca gaataaaggt
     6961 tgttttgaaa ctgaa
//
