LOCUS       NM_052880               2420 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens phosphoinositide-3-kinase interacting protein 1
            (PIK3IP1), transcript variant 1, mRNA.
ACCESSION   NM_052880
VERSION     NM_052880.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Lei,K., Liang,R., Liang,J., Lu,N., Huang,J., Xu,K., Tan,B.,
            Wang,K., Liang,Y., Wang,W., Lin,H. and Wang,M.
  TITLE     CircPDE5A-encoded novel regulator of the PI3K/AKT pathway inhibits
            esophageal squamous cell carcinoma progression by promoting
            USP14-mediated de-ubiquitination of PIK3IP1
  JOURNAL   J Exp Clin Cancer Res 43 (1), 124 (2024)
   PUBMED   38658954
  REMARK    GeneRIF: CircPDE5A-encoded novel regulator of the PI3K/AKT pathway
            inhibits esophageal squamous cell carcinoma progression by
            promoting USP14-mediated de-ubiquitination of PIK3IP1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Chen,L., Cao,Y., Shen,Y., Li,H., Ye,R. and Yao,J.
  TITLE     Downregulation of PIK3IP1 in retinal microglia promotes retinal
            pathological neovascularization via PI3K-AKT pathway activation
  JOURNAL   Sci Rep 13 (1), 12754 (2023)
   PUBMED   37550343
  REMARK    GeneRIF: Downregulation of PIK3IP1 in retinal microglia promotes
            retinal pathological neovascularization via PI3K-AKT pathway
            activation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2420)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2420)
  AUTHORS   Chen,Y., Wang,J., Wang,X., Li,X., Song,J., Fang,J., Liu,X., Liu,T.,
            Wang,D., Li,Q., Wen,S., Ma,D., Xia,J., Luo,L., Zheng,S.G., Cui,J.,
            Zeng,G., Chen,L., Cheng,B. and Wang,Z.
  TITLE     Pik3ip1 Is a Negative Immune Regulator that Inhibits Antitumor
            T-Cell Immunity
  JOURNAL   Clin Cancer Res 25 (20), 6180-6194 (2019)
   PUBMED   31350312
  REMARK    GeneRIF: Pik3ip1 Is a Negative Immune Regulator that Inhibits
            Antitumor T-Cell Immunity.
            Erratum:[Clin Cancer Res. 2022 May 13;28(10):2197. doi:
            10.1158/1078-0432.CCR-22-1109.. PMID: 35553648]
REFERENCE   5  (bases 1 to 2420)
  AUTHORS   Zhao,J. and Cheng,L.
  TITLE     Long non-coding RNA CCAT1/miR-148a axis promotes osteosarcoma
            proliferation and migration through regulating PIK3IP1
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 49 (6), 503-512 (2017)
   PUBMED   28549102
  REMARK    GeneRIF: Findings indicate that colon cancer associated transcript
            1 (non-protein coding) (CCAT1) was upregulated in osteosarcoma
            tissues and cells, and was involved in the proliferation and
            migration of osteosarcoma via regulating miR-148a/phosphatidyl
            inositol 3-kinase interacting protein 1 (PIK3IP1) signal pathway.
REFERENCE   6  (bases 1 to 2420)
  AUTHORS   Zhu,Z., He,X., Johnson,C., Stoops,J., Eaker,A.E., Stoffer,D.S.,
            Bell,A., Zarnegar,R. and DeFrances,M.C.
  TITLE     PI3K is negatively regulated by PIK3IP1, a novel p110 interacting
            protein
  JOURNAL   Biochem Biophys Res Commun 358 (1), 66-72 (2007)
   PUBMED   17475214
  REMARK    GeneRIF: PIK3IP1 directly binds to the p110 catalytic subunit and
            down modulates PI3K activity. Suggested that PIK3IP1 is a new type
            of PI3K regulator.
REFERENCE   7  (bases 1 to 2420)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   8  (bases 1 to 2420)
  AUTHORS   Collins,J.E., Wright,C.L., Edwards,C.A., Davis,M.P., Grinham,J.A.,
            Cole,C.G., Goward,M.E., Aguado,B., Mallya,M., Mokrab,Y.,
            Huckle,E.J., Beare,D.M. and Dunham,I.
  TITLE     A genome annotation-driven approach to cloning the human ORFeome
  JOURNAL   Genome Biol 5 (10), R84 (2004)
   PUBMED   15461802
REFERENCE   9  (bases 1 to 2420)
  AUTHORS   Collins,J.E., Goward,M.E., Cole,C.G., Smink,L.J., Huckle,E.J.,
            Knowles,S., Bye,J.M., Beare,D.M. and Dunham,I.
  TITLE     Reevaluating human gene annotation: a second-generation analysis of
            chromosome 22
  JOURNAL   Genome Res 13 (1), 27-36 (2003)
   PUBMED   12529303
REFERENCE   10 (bases 1 to 2420)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC412134.1, AC002073.1 and AW005175.1.
            
            On May 17, 2019 this sequence version replaced NM_052880.4.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.172524.1,
                                           SRR7346977.1323227.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2147920
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000215912.10/ ENSP00000215912.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-516               DC412134.1         47-562
            517-652             AC002073.1         17253-17388
            653-731             AC002073.1         17494-17572
            732-2349            AC002073.1         23599-25216
            2350-2420           AW005175.1         13-83               c
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.2"
     gene            1..2420
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /note="phosphoinositide-3-kinase interacting protein 1"
                     /db_xref="GeneID:113791"
                     /db_xref="HGNC:HGNC:24942"
                     /db_xref="MIM:619158"
     exon            1..214
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..936
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; kringle domain-containing protein HGFL;
                     transmembrane inhibitor of PI3K; PI3K-interacting protein
                     1"
                     /codon_start=1
                     /product="phosphoinositide-3-kinase-interacting protein 1
                     isoform 1 precursor"
                     /protein_id="NP_443112.2"
                     /db_xref="CCDS:CCDS13893.1"
                     /db_xref="GeneID:113791"
                     /db_xref="HGNC:HGNC:24942"
                     /db_xref="MIM:619158"
                     /translation="MLLAWVQAFLVSNMLLAEAYGSGGCFWDNGHLYREDQTSPAPGL
                     RCLNWLDAQSGLASAPVSGAGNHSYCRNPDEDPRGPWCYVSGEAGVPEKRPCEDLRCP
                     ETTSQALPAFTTEIQEASEGPGADEVQVFAPANALPARSEAAAVQPVIGISQRVRMNS
                     KEKKDLGTLGYVLGITMMVIIIAIGAGIILGYSYKRGKDLKEQHDQKVCEREMQRITL
                     PLSAFTNPTCEIVDEKTVVVHTSQTPVDPQEGTTPLMGQAGTPGA"
     exon            215..331
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /inference="alignment:Splign:2.1.0"
     exon            332..451
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /inference="alignment:Splign:2.1.0"
     exon            452..652
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /inference="alignment:Splign:2.1.0"
     exon            653..731
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /inference="alignment:Splign:2.1.0"
     exon            732..2420
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1379..1384
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /note="hexamer: AATATA"
     polyA_site      1400
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
     regulatory      2318..2323
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /note="hexamer: TATAAA"
     polyA_site      2337
                     /gene="PIK3IP1"
                     /gene_synonym="HGFL; hHGFL(S); TrIP"
                     /note="major polyA site"
ORIGIN      
        1 agttctaaag agaggctgtt taccagaaca gcataacaag ggcaggtctg actgcaaggc
       61 tgggactggg aggcagagcc gccgccaagg gggcctcggt taaacactgg tcgttcaatc
      121 acctgcaaga cgaaggaggc aaggatgctg ttggcctggg tacaagcatt cctcgtcagc
      181 aacatgctcc tagcagaagc ctatggatct ggaggctgtt tctgggacaa cggccacctg
      241 taccgggagg accagacctc ccccgcgccg ggcctccgct gcctcaactg gctggacgcg
      301 cagagcgggc tggcctcggc ccccgtgtcg ggggccggca atcacagtta ctgccgaaac
      361 ccggacgagg acccgcgcgg gccctggtgc tacgtcagtg gcgaggccgg cgtccctgag
      421 aaacggcctt gcgaggacct gcgctgtcca gagaccacct cccaggccct gccagccttc
      481 acgacagaaa tccaggaagc gtctgaaggg ccaggtgcag atgaggtgca ggtgttcgct
      541 cctgccaacg ccctgcccgc tcggagtgag gcggcagctg tgcagccagt gattgggatc
      601 agccagcggg tgcggatgaa ctccaaggag aaaaaggacc tgggaactct gggctacgtg
      661 ctgggcatta ccatgatggt gatcatcatt gccatcggag ctggcatcat cttgggctac
      721 tcctacaaga gggggaagga tttgaaagaa cagcatgatc agaaagtatg tgagagggag
      781 atgcagcgaa tcactctgcc cttgtctgcc ttcaccaacc ccacctgtga gattgtggat
      841 gagaagactg tcgtggtcca caccagccag actccagttg accctcagga gggcaccacc
      901 ccccttatgg gccaggccgg gactcctggg gcctgagccc ccccagtggg caggagccca
      961 tgcagacact ggtgcaggac agcccaccct cctacagcta ggaggaacta ccactttgtg
     1021 ttctggttaa aaccctacca ctcccccgct tttttggcga atcctagtaa gagtgacaga
     1081 agcaggtggc cctgtgggct gagggtaagg ctgggtaggg tcctaacagt gctccttgtc
     1141 catcccttgg agcagatttt gtctgtggat ggagacagtg gcagctccca cagtgatgct
     1201 gctgctaagg gcttccaaac attgcctgca cccctggaac tgaaccaggg atagacgggg
     1261 agctccccca ggctcctctg tgctttacta agatggcctc agtctccact gtgggcttga
     1321 gtggcataca ctgttattca tggttaaggt aaagcaggtc aagggatggc attgaaaaaa
     1381 tatatttagt ttttaaaata tttgggatgg aactccctac tgacctctga gaactggaaa
     1441 cgagtttgta cagaagtcag aactttgggt tgggaatgag atctaggttg tggctgctgg
     1501 tatgcttcag cttgctggca atgatgtgcc ttgacaaccg tgggccaggc ctgggcccag
     1561 ggactcttcc tgtttcataa ggaaaggaag aattgcactg agcattccac ttaggaagag
     1621 gatagagaag gatctgctcc gcctttggcc acaggagcag aggcagacct gggatgcccc
     1681 agtttctctt cagggatgga tagtgacctg tcttcatttt gcacaggtaa gagagtagtt
     1741 agctaaccta tgggaattat actgtggggc cttgtgagct gcttctaaga ggctaacctg
     1801 gaaactaagc tcagaggcaa ggtaataaag cacttcaggg cttgctcccc aagtgggcct
     1861 gatttagcag gtggtcctgc gggcgtccag gtcagcacct tcctgtaggg cactggggct
     1921 agggtcacag cccctaactc ataaagcaat caaagaacca ttagaaaggg ctcattaagc
     1981 cttttggaca caggacccca gagaggaaaa agtgacttgc ccaaggtcgt aagcaagcta
     2041 ctggcatggc aagagcccag cttcctgacg gagcgcaaca tttctccact gcactgtgct
     2101 agcagctcag cagggcctct aacctgtgat gtcacactca agaggccttg gcagctccta
     2161 gccatagagc ttcctttcca gaacccttcc actgcccaat gtggagacgg gttagtgggg
     2221 ctttctatgg agccatctgc tttggggacc tagacctcag gtggtctctt ggtgttagtg
     2281 atgctggaga agagaatatt actggtttct acttttctat aaaggcattt ctctatatac
     2341 atgttttata tacctcattc tgacacctgc atatagtgtg ggaaattgct ctgcatttga
     2401 cttaattaaa aaaaaaaaaa
//
