LOCUS       NM_006219               6259 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens phosphatidylinositol-4,5-bisphosphate 3-kinase
            catalytic subunit beta (PIK3CB), transcript variant 1, mRNA.
ACCESSION   NM_006219 XM_005247530
VERSION     NM_006219.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Liu,L., Feng,X., Fan,C., Kong,D., Feng,X., Sun,C., Xu,Y., Li,B.,
            Jiang,Y. and Zheng,C.
  TITLE     PDCD4 interacting with PIK3CB and CTSZ promotes the apoptosis of
            multiple myeloma cells
  JOURNAL   FASEB J 38 (16), e70024 (2024)
   PUBMED   39190024
  REMARK    GeneRIF: PDCD4 interacting with PIK3CB and CTSZ promotes the
            apoptosis of multiple myeloma cells.
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Liu,G., Hou,Y., Jin,X., Zhang,Y., Sun,C., Huang,C., Ren,Y., Gao,J.,
            Wang,X. and Jiang,X.
  TITLE     PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote
            erythropoiesis and megakaryopoiesis
  JOURNAL   Elife 13, 95815 (2024)
   PUBMED   38757931
  REMARK    GeneRIF: PI3K/HSCB axis facilitates FOG1 nuclear translocation to
            promote erythropoiesis and megakaryopoiesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6259)
  AUTHORS   Duewell,B.R., Wilson,N.E., Bailey,G.M., Peabody,S.E. and
            Hansen,S.D.
  TITLE     Molecular dissection of PI3Kbeta synergistic activation by receptor
            tyrosine kinases, GbetaGgamma, and Rho-family GTPases
  JOURNAL   Elife 12, 88991 (2024)
   PUBMED   38713746
  REMARK    GeneRIF: Molecular dissection of PI3Kbeta synergistic activation by
            receptor tyrosine kinases, GbetaGgamma, and Rho-family GTPases.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6259)
  AUTHORS   Suh,K.J., Ryu,M.H., Zang,D.Y., Bae,W.K., Lee,H.S., Oh,H.J.,
            Kang,M., Kim,J.W., Kim,B.J., Mortimer,P.G.S., Kim,H.J. and Lee,K.W.
  TITLE     AZD8186 in Combination With Paclitaxel in Patients With Advanced
            Gastric Cancer: Results From a Phase Ib/II Study (KCSG ST18-20)
  JOURNAL   Oncologist 28 (9), e823-e834 (2023)
   PUBMED   37036671
  REMARK    GeneRIF: AZD8186 in Combination With Paclitaxel in Patients With
            Advanced Gastric Cancer: Results From a Phase Ib/II Study (KCSG
            ST18-20).
            Erratum:[Oncologist. 2024 Apr 4;29(4):e579. doi:
            10.1093/oncolo/oyad287.. PMID: 37815173]
REFERENCE   5  (bases 1 to 6259)
  AUTHORS   Xu,W., Chen,Y., Zhang,Z., Jiang,Y. and Wang,Z.
  TITLE     Exosomal PIK3CB promotes PD-L1 expression and malignant
            transformation in esophageal squamous cell carcinoma
  JOURNAL   Med Oncol 40 (8), 221 (2023)
   PUBMED   37402056
  REMARK    GeneRIF: Exosomal PIK3CB promotes PD-L1 expression and malignant
            transformation in esophageal squamous cell carcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6259)
  AUTHORS   Milani,D., Mazzoni,M., Borgatti,P., Zauli,G., Cantley,L. and
            Capitani,S.
  TITLE     Extracellular human immunodeficiency virus type-1 Tat protein
            activates phosphatidylinositol 3-kinase in PC12 neuronal cells
  JOURNAL   J Biol Chem 271 (38), 22961-22964 (1996)
   PUBMED   8798481
REFERENCE   7  (bases 1 to 6259)
  AUTHORS   Bonnefoy-Berard,N., Liu,Y.C., von Willebrand,M., Sung,A., Elly,C.,
            Mustelin,T., Yoshida,H., Ishizaka,K. and Altman,A.
  TITLE     Inhibition of phosphatidylinositol 3-kinase activity by association
            with 14-3-3 proteins in T cells
  JOURNAL   Proc Natl Acad Sci U S A 92 (22), 10142-10146 (1995)
   PUBMED   7479742
REFERENCE   8  (bases 1 to 6259)
  AUTHORS   Hu,P. and Schlessinger,J.
  TITLE     Direct association of p110 beta phosphatidylinositol 3-kinase with
            p85 is mediated by an N-terminal fragment of p110 beta
  JOURNAL   Mol Cell Biol 14 (4), 2577-2583 (1994)
   PUBMED   8139559
REFERENCE   9  (bases 1 to 6259)
  AUTHORS   Hu,P., Mondino,A., Skolnik,E.Y. and Schlessinger,J.
  TITLE     Cloning of a novel, ubiquitously expressed human
            phosphatidylinositol 3-kinase and identification of its binding
            site on p85
  JOURNAL   Mol Cell Biol 13 (12), 7677-7688 (1993)
   PUBMED   8246984
REFERENCE   10 (bases 1 to 6259)
  AUTHORS   Carpenter,C.L., Duckworth,B.C., Auger,K.R., Cohen,B.,
            Schaffhausen,B.S. and Cantley,L.C.
  TITLE     Purification and characterization of phosphoinositide 3-kinase from
            rat liver
  JOURNAL   J Biol Chem 265 (32), 19704-19711 (1990)
   PUBMED   2174051
  REMARK    GeneRIF: This paper reports the first purification of a
            phosphoinositide 3-kinase and shows that the protein is a
            heterodimer of an 85 kd regulatory subunit that mediates binding to
            phosphorylated proteins and a 110 kd catalytic subunit.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC067728.25, AC117389.5, BC114432.1, AC020890.22 and AI623206.1.
            
            On or before Jul 5, 2019 this sequence version replaced
            XM_005247530.2, NM_006219.2.
            
            Summary: This gene encodes an isoform of the catalytic subunit of
            phosphoinositide 3-kinase (PI3K). These kinases are important in
            signaling pathways involving receptors on the outer membrane of
            eukaryotic cells and are named for their catalytic subunit. The
            encoded protein is the catalytic subunit for PI3Kbeta (PI3KB).
            PI3KB has been shown to be part of the activation pathway in
            neutrophils which have bound immune complexes at sites of injury or
            infection. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Dec 2011].
            
            Transcript Variant: This variant (1) represent the longer
            transcript and encodes the longer protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.4019984.1,
                                           SRR11853562.23969.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000674063.1/ ENSP00000501150.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-234               AC067728.25        40833-41066         c
            235-339             AC067728.25        2601-2705           c
            340-355             AC117389.5         36526-36541
            356-3568            BC114432.1         5-3217
            3569-5876           AC020890.22        31397-33704
            5877-6259           AI623206.1         12-394              c
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q22.3"
     gene            1..6259
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /note="phosphatidylinositol-4,5-bisphosphate 3-kinase
                     catalytic subunit beta"
                     /db_xref="GeneID:5291"
                     /db_xref="HGNC:HGNC:8976"
                     /db_xref="MIM:602925"
     exon            1..234
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            235..339
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            340..526
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     CDS             356..3568
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.1.153"
                     /note="isoform 1 is encoded by transcript variant 1;
                     PI3-kinase p110 subunit beta; PtdIns-3-kinase p110;
                     PI3K-beta; PI3-kinase subunit beta; ptdIns-3-kinase
                     subunit beta; ptdIns-3-kinase subunit p110-beta;
                     phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa
                     catalytic subunit beta; serine/threonine protein kinase
                     PIK3CB; phosphoinositide-3-kinase, catalytic, beta
                     polypeptide"
                     /codon_start=1
                     /product="phosphatidylinositol 4,5-bisphosphate 3-kinase
                     catalytic subunit beta isoform isoform 1"
                     /protein_id="NP_006210.1"
                     /db_xref="CCDS:CCDS3104.1"
                     /db_xref="GeneID:5291"
                     /db_xref="HGNC:HGNC:8976"
                     /db_xref="MIM:602925"
                     /translation="MCFSFIMPPAMADILDIWAVDSQIASDGSIPVDFLLPTGIYIQL
                     EVPREATISYIKQMLWKQVHNYPMFNLLMDIDSYMFACVNQTAVYEELEDETRRLCDV
                     RPFLPVLKLVTRSCDPGEKLDSKIGVLIGKGLHEFDSLKDPEVNEFRRKMRKFSEEKI
                     LSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNM
                     NPIKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNR
                     ALPHFILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQ
                     IVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTIVSSEVSGKNDHIWNEPLEFDINIC
                     DLPRMARLCFAVYAVLDKVKTKKSTKTINPSKYQTIRKAGKVHYPVAWVNTMVFDFKG
                     QLRTGDIILHSWSSFPDELEEMLNPMGTVQTNPYTENATALHVKFPENKKQPYYYPPF
                     DKIIEKAAEIASSDSANVSSRGGKKFLPVLKEILDRDPLSQLCENEMDLIWTLRQDCR
                     EIFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAV
                     GCLRQMSDEELSQYLLQLVQVLKYEPFLDCALSRFLLERALGNRRIGQFLFWHLRSEV
                     HIPAVSVQFGVILEAYCRGSVGHMKVLSKQVEALNKLKTLNSLIKLNAVKLNRAKGKE
                     AMHTCLKQSAYREALSDLQSPLNPCVILSELYVEKCKYMDSKMKPLWLVYNNKVFGED
                     SVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVST
                     SETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYV
                     LGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGK
                     TGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLAL
                     GKSEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS"
     exon            527..752
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            753..976
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1156
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1327
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1405
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1657
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1754
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..1885
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1936
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2125
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2247
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2391
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            2392..2491
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2670
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..2780
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            2781..2859
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            2860..3027
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3151
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            3152..3297
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            3298..3430
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     exon            3431..6259
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4902..4907
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /note="hexamer: AATAAA"
     polyA_site      4937
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /note="major polyA site"
     regulatory      6238..6243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
                     /note="hexamer: ATTAAA"
     polyA_site      6259
                     /gene="PIK3CB"
                     /gene_synonym="P110BETA; PI3K; PI3KBETA; PIK3C1"
ORIGIN      
        1 gcacacgggc gcgggttcgg cggcggcgcg cgcgtgctct gtcggcctgt gcggcgctgc
       61 ggcggagcgg gccatggcag tggggagggg gcgagtgtag tgctgcgcgg ggcaggcggg
      121 aggtgatcga gagaggcagg gatgggggcg ccggagtgga gcggttgcgg cggcctgggc
      181 tgctgactgc gcacttggaa tagtagcagg cggcggcggc ggaacgccag gcagtgtatg
      241 ttttaactgg aaaaagtctt ccatgaaaac gtcactttta aaaaataagt aatgccatct
      301 gttttcctaa aagactgaaa tgggggcgac cattcttagg gccccgtggt tatgaatgtg
      361 cttcagtttc ataatgcctc ctgctatggc agacatcctt gacatctggg cggtggattc
      421 acagatagca tctgatggct ccatacctgt ggatttcctt ttgcccactg ggatttatat
      481 ccagttggag gtacctcggg aagctaccat ttcttatatt aagcagatgt tatggaagca
      541 agttcacaat tacccaatgt tcaacctcct tatggatatt gactcctata tgtttgcatg
      601 tgtgaatcag actgctgtat atgaggagct tgaagatgaa acacgaagac tctgtgatgt
      661 cagacctttt cttccagttc tcaaattagt gacaagaagt tgtgacccag gggaaaaatt
      721 agactcaaaa attggagtcc ttataggaaa aggtctgcat gaatttgatt ccttgaagga
      781 tcctgaagta aatgaatttc gaagaaaaat gcgcaaattc agcgaggaaa aaatcctgtc
      841 acttgtggga ttgtcttgga tggactggct aaaacaaaca tatccaccag agcatgaacc
      901 atccatccct gaaaacttag aagataaact ttatggggga aagctcatcg tagctgttca
      961 ttttgaaaac tgccaggacg tgtttagctt tcaagtgtct cctaatatga atcctatcaa
     1021 agtaaatgaa ttggcaatcc aaaaacgttt gactattcat gggaaggaag atgaagttag
     1081 cccctatgat tatgtgttgc aagtcagcgg gagagtagaa tatgtttttg gtgatcatcc
     1141 actaattcag ttccagtata tccggaactg tgtgatgaac agagccctgc cccattttat
     1201 acttgtggaa tgctgcaaga tcaagaaaat gtatgaacaa gaaatgattg ccatagaggc
     1261 tgccataaat cgaaattcat ctaatcttcc tcttccatta ccaccaaaga aaacacgaat
     1321 tatttctcat gtttgggaaa ataacaaccc tttccaaatt gtcttggtta agggaaataa
     1381 acttaacaca gaggaaactg taaaagttca tgtcagggct ggtctttttc atggtactga
     1441 gctcctgtgt aaaaccatcg taagctcaga ggtatcaggg aaaaatgatc atatttggaa
     1501 tgaaccactg gaatttgata ttaatatttg tgacttacca agaatggctc gattatgttt
     1561 tgctgtttat gcagttttgg ataaagtaaa aacgaagaaa tcaacgaaaa ctattaatcc
     1621 ctctaaatat cagaccatca ggaaagctgg aaaagtgcat tatcctgtag cgtgggtaaa
     1681 tacgatggtt tttgacttta aaggacaatt gagaactgga gacataatat tacacagctg
     1741 gtcttcattt cctgatgaac tcgaagaaat gttgaatcca atgggaactg ttcaaacaaa
     1801 tccatatact gaaaatgcaa cagctttgca tgttaaattt ccagagaata aaaaacaacc
     1861 ttattattac cctcccttcg ataagattat tgaaaaggca gctgagattg caagcagtga
     1921 tagtgctaat gtgtcaagtc gaggtggaaa aaagtttctt cctgtattga aagaaatctt
     1981 ggacagggat cccttgtctc aactgtgtga aaatgaaatg gatcttattt ggactttgcg
     2041 acaagactgc cgagagattt tcccacaatc actgccaaaa ttactgctgt caatcaagtg
     2101 gaataaactt gaggatgttg ctcagcttca ggcgctgctt cagatttggc ctaaactgcc
     2161 cccccgggag gccctagagc ttctggattt caactatcca gaccagtacg ttcgagaata
     2221 tgctgtaggc tgcctgcgac agatgagtga tgaagaactt tctcaatatc ttttacaact
     2281 ggtgcaagtg ttaaaatatg agccttttct tgattgtgcc ctctctagat tcctattaga
     2341 aagagcactt ggtaatcgga ggatagggca gtttctattt tggcatctta ggtcagaagt
     2401 gcacattcct gctgtctcag tacaatttgg tgtcatcctt gaagcatact gccggggaag
     2461 tgtggggcac atgaaagtgc tttctaagca ggttgaagca ctcaataagt taaaaacttt
     2521 aaatagttta atcaaactga atgccgtgaa gttaaacaga gccaaaggga aggaggccat
     2581 gcatacctgt ttaaaacaga gtgcttaccg ggaagccctc tctgacctgc agtcacccct
     2641 gaacccatgt gttatcctct cagaactcta tgttgaaaag tgcaaataca tggattccaa
     2701 aatgaagcct ttgtggctgg tatacaataa caaggtattt ggtgaggatt cagttggagt
     2761 gatttttaaa aatggtgatg atttacgaca ggatatgttg acactccaaa tgttgcgctt
     2821 gatggattta ctctggaaag aagctggttt ggatcttcgg atgttgcctt atggctgttt
     2881 agcaacagga gatcgctctg gcctcattga agttgtgagc acctctgaaa caattgctga
     2941 cattcagctg aacagtagca atgtggctgc tgcagcagcc ttcaacaaag atgcccttct
     3001 gaactggctt aaagaataca actctgggga tgacctggac cgagccattg aggaatttac
     3061 actgtcctgt gctggctact gtgtagcttc ttatgtcctt gggattggtg acagacatag
     3121 tgacaacatc atggtcaaaa aaactggcca gctcttccac attgactttg gacatattct
     3181 tggaaatttc aaatctaagt ttggcattaa aagggagcga gtgcctttta ttcttaccta
     3241 tgatttcatc catgtcattc aacaaggaaa aacaggaaat acagaaaagt ttggccggtt
     3301 ccgccagtgt tgtgaggatg catatctgat tttacgacgg catgggaatc tcttcatcac
     3361 tctctttgcg ctgatgttga ctgcagggct tcctgaactc acatcagtca aagatataca
     3421 gtatcttaag gactctcttg cattagggaa gagtgaagaa gaagcactca aacagtttaa
     3481 gcaaaaattt gatgaggcgc tcagggaaag ctggactact aaagtgaact ggatggccca
     3541 cacagttcgg aaagactaca gatcttaacg atcagccttc gctcctaatg tatttgttgg
     3601 tttcatttca ttttcatttt gcacttgcac taaattgaac atgaccctgt tagagatgtt
     3661 ataaagggaa tgaaatcctg gaactcagag ttaaattaag aacaaggcat cccacagaac
     3721 ctaatctgaa caatccccga tgattccctc tgctttttga atgcttccaa gacttatcat
     3781 gaaaactgtc aatggataat catttcctgc tgactttgca cgccaaggaa tgctactagg
     3841 gattgtttcc gtttttgttt gttttttcta atatttggta cttcccagaa tggtgtaaat
     3901 acttcttttc aatgttgtga ccaagtattg tcactcagcc aacaactttt ccacacctgg
     3961 gggttggtgg ctgttcttac tgtccaaatg aagctaaaaa gaaaggcatc tttcttccct
     4021 tttaaaattg tgtaaactgc aaattataat ataatttgaa tttatgatta ttttccagaa
     4081 gaaatcttgt aaacctgtgg atactcatta attcttttgt taatatttat ttccatgata
     4141 gcatcattcc agccagactt gctgaaaatc tactggtgag gcaaatataa tatatataaa
     4201 tatgctacat atatatttat aaaatttcta gtgggagttc tatataaatg tttctttggt
     4261 attcttcagc ctgtgattta aagttttaca aaaagcagag ctttttccta agttactttt
     4321 cagttaggta actgtgtgat ccagttcttc cagctgcttc tataatgagg cacatattaa
     4381 tacagttttt atatggtatc tatgaaagag ttcacttcat agagaataat acttgagcaa
     4441 atgtatccaa gaaagcaagc aaatgaaaag aaacctattt atggaataaa ctccagatct
     4501 gaaattcagt attttagaaa aatgccagct cttcttactg tatttattaa aacttgtaat
     4561 aatgtgattt ttttcaagga tattagttca aattgaaatg gtttcacgcc acacggaaat
     4621 ctttaagtta tttgttgagg taccatatat ttagggtgct aggggcaagt aatgttaata
     4681 tgtgcaatag gaactactgg tttgaatgtg taaatgggtg atctctctga gtcctggcaa
     4741 catccagcaa aactactgct tattctccaa agaatattgg gagctctcaa tcctcggtga
     4801 tatgggaaag agaactgagt atttgcccta tgactgagct ttctatagga attttattaa
     4861 agaatgttta attttgttgt ccttcttaat gttctcagtc aaataaatga gtgagctggt
     4921 ttcggctgct cttggaatgg gtgagcctct tctttatggg tagactgggc ctttggaact
     4981 tggcactgga actccaagaa atggccaagt cagtagacaa accaacctca ggaataggct
     5041 aaggcttatt atggcctctt ccctgacttc tccccttgtt tcccagcctc atcaggcatg
     5101 gtataggagg ccccctggac tttggtggga gcctgaggta aggagccatg catatgggag
     5161 gtgtcctgaa gtctgggtag ttacttggca ctgagccaag gccagactct gctgctttgg
     5221 agctcttgtt catggggcag atgctggagc agtccagttc cttggaaata actcagctga
     5281 ggatgggagt tggcccctga attcctcatt tccagggctg gtgtagactc actgagactt
     5341 ccaggaatag aactatggaa ggacaggttt gttcagagat ctttgtctag tagccaccca
     5401 ccatttcatg aaccaggccg caggtcagtg gtttggagaa tggtgaacac tgccaggaag
     5461 aaatggatac cattctttcc agaggggtct cctcagccaa aaggagggcc ttgataaata
     5521 catgccaaat cagtgaagtt caagtcaact gtttttccca tatgggcacc aaattgtatc
     5581 tttcctgttt tctttgaagg gttaagtaac gtgaccatag tcacagagta gttgatggag
     5641 ccagtattca aacccagaaa gtaagaagcc tattttaatt atctgtgctc tttactcaca
     5701 atgcctcagt atacatttca gatttattgg gttccacaaa tagaaatcta tggaaatttt
     5761 gaatcaaatt gcattaagct atagacaagg ttgagacaaa ttgacatctc cagaatattg
     5821 agttttccaa aatatgtaag tggagtaccc atttatttag attatctttc atttatatcc
     5881 gcaatgattt atagtattct gtgtttacat attatgcatc gtttgttaga ttcctgggta
     5941 agtgacttta ttgtaagttt cattgttgtt aatctatagc aatcattctc caagtgtggt
     6001 cccctgatga ggagcatcag catcaccaga gagaactggt taaaaatgca aattcttaat
     6061 ttttaatttt tgtgagtaca tagtagatat atatatgggg tacatggaat attttgatac
     6121 aggcatataa catgtaatta tcgcatcagg gtaattgggg tatccattac ctcaagcatt
     6181 tatcctttgt attagaaaca atccagttat actcttttag ttattttaag atctataatt
     6241 aaattatcga ctatagtta
//
