LOCUS       NM_002647               9415 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens phosphatidylinositol 3-kinase catalytic subunit type 3
            (PIK3C3), transcript variant 1, mRNA.
ACCESSION   NM_002647
VERSION     NM_002647.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Gong,M., Peng,C., Yang,C., Wang,Z., Qian,H., Hu,X., Zhou,P.,
            Shan,C. and Ding,Q.
  TITLE     Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as
            crucial entry factors for Ebola virus infection
  JOURNAL   PLoS Pathog 20 (8), e1012444 (2024)
   PUBMED   39173055
  REMARK    GeneRIF: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and
            PIK3C3 as crucial entry factors for Ebola virus infection.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Tahoun,M. and Sadaka,A.S.
  TITLE     Deregulated expression of autophagy genes; PIK3C3 and RAB7A in
            COVID-19 patients
  JOURNAL   Hum Immunol 85 (3), 110801 (2024)
   PUBMED   38609772
  REMARK    GeneRIF: Deregulated expression of autophagy genes; PIK3C3 and
            RAB7A in COVID-19 patients.
REFERENCE   3  (bases 1 to 9415)
  AUTHORS   Song,W., Postoak,J.L., Wu,L. and Van Kaer,L.
  TITLE     PIK3C3/VPS34 keeps body fats healthy
  JOURNAL   Autophagy 19 (8), 2398-2400 (2023)
   PUBMED   36629752
  REMARK    GeneRIF: PIK3C3/VPS34 keeps body fats healthy.
REFERENCE   4  (bases 1 to 9415)
  AUTHORS   Jia,M., Yue,X., Sun,W., Zhou,Q., Chang,C., Gong,W., Feng,J., Li,X.,
            Zhan,R., Mo,K., Zhang,L., Qian,Y., Sun,Y., Wang,A., Zou,Y.,
            Chen,W., Li,Y., Huang,L., Yang,Y., Zhao,Y. and Cheng,X.
  TITLE     ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase
            activity by its lactylation
  JOURNAL   Sci Adv 9 (22), eadg4993 (2023)
   PUBMED   37267363
  REMARK    GeneRIF: ULK1-mediated metabolic reprogramming regulates Vps34
            lipid kinase activity by its lactylation.
REFERENCE   5  (bases 1 to 9415)
  AUTHORS   Cain,R.J., Scortti,M., Monzo,H.J. and Vazquez-Boland,J.A.
  TITLE     Listeria InlB Expedites Vacuole Escape and Intracellular
            Proliferation by Promoting Rab7 Recruitment via Vps34
  JOURNAL   mBio 14 (1), e0322122 (2023)
   PUBMED   36656016
  REMARK    GeneRIF: Listeria InlB Expedites Vacuole Escape and Intracellular
            Proliferation by Promoting Rab7 Recruitment via Vps34.
REFERENCE   6  (bases 1 to 9415)
  AUTHORS   Borgatti,P., Zauli,G., Colamussi,M.L., Gibellini,D., Previati,M.,
            Cantley,L.L. and Capitani,S.
  TITLE     Extracellular HIV-1 Tat protein activates phosphatidylinositol 3-
            and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells
  JOURNAL   Eur J Immunol 27 (11), 2805-2811 (1997)
   PUBMED   9394803
REFERENCE   7  (bases 1 to 9415)
  AUTHORS   Panaretou,C., Domin,J., Cockcroft,S. and Waterfield,M.D.
  TITLE     Characterization of p150, an adaptor protein for the human
            phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by
            phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase
            complex
  JOURNAL   J Biol Chem 272 (4), 2477-2485 (1997)
   PUBMED   8999962
REFERENCE   8  (bases 1 to 9415)
  AUTHORS   Milani,D., Mazzoni,M., Borgatti,P., Zauli,G., Cantley,L. and
            Capitani,S.
  TITLE     Extracellular human immunodeficiency virus type-1 Tat protein
            activates phosphatidylinositol 3-kinase in PC12 neuronal cells
  JOURNAL   J Biol Chem 271 (38), 22961-22964 (1996)
   PUBMED   8798481
REFERENCE   9  (bases 1 to 9415)
  AUTHORS   Volinia,S., Dhand,R., Vanhaesebroeck,B., MacDougall,L.K., Stein,R.,
            Zvelebil,M.J., Domin,J., Panaretou,C. and Waterfield,M.D.
  TITLE     A human phosphatidylinositol 3-kinase complex related to the yeast
            Vps34p-Vps15p protein sorting system
  JOURNAL   EMBO J 14 (14), 3339-3348 (1995)
   PUBMED   7628435
REFERENCE   10 (bases 1 to 9415)
  AUTHORS   Vogel,L.B. and Fujita,D.J.
  TITLE     The SH3 domain of p56lck is involved in binding to
            phosphatidylinositol 3'-kinase from T lymphocytes
  JOURNAL   Mol Cell Biol 13 (12), 7408-7417 (1993)
   PUBMED   7504174
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB450556.1, BC053651.1, CA945171.1 and AC087683.5.
            
            On Nov 23, 2018 this sequence version replaced NM_002647.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.226042.1,
                                           SRR1803617.105756.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262039.9/ ENSP00000262039.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                DB450556.1         38-71
            35-2992             BC053651.1         1-2958
            2993-3069           CA945171.1         15-91               c
            3070-9415           AC087683.5         26453-32798         c
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.3"
     gene            1..9415
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /note="phosphatidylinositol 3-kinase catalytic subunit
                     type 3"
                     /db_xref="GeneID:5289"
                     /db_xref="HGNC:HGNC:8974"
                     /db_xref="MIM:602609"
     exon            1..126
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..2722
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /EC_number="2.7.1.137"
                     /note="isoform 1 is encoded by transcript variant 1; PI3K
                     type 3; PI3-kinase type 3; PtdIns-3-kinase type 3;
                     phosphatidylinositol 3-kinase p100 subunit;
                     phosphoinositide-3-kinase, class 3; vacuolar protein
                     sorting 34 homolog"
                     /codon_start=1
                     /product="phosphatidylinositol 3-kinase catalytic subunit
                     type 3 isoform 1"
                     /protein_id="NP_002638.2"
                     /db_xref="CCDS:CCDS11920.1"
                     /db_xref="GeneID:5289"
                     /db_xref="HGNC:HGNC:8974"
                     /db_xref="MIM:602609"
                     /translation="MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPML
                     KFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPR
                     NAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKTP
                     GRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLM
                     VEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARS
                     LRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFL
                     KCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDL
                     LMYLLQLVQALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPL
                     PSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQ
                     RDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQRESGNR
                     KKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFK
                     TEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFM
                     QFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILG
                     VGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEF
                     RKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAV
                     HYMQSLIDESVHALFAAVVEQIHKFAQYWRK"
     exon            127..315
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            316..459
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            460..589
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            590..676
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            677..772
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            773..844
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            845..949
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            950..1042
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1228
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1383
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1474
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1542
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1648
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1765
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1897
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..2026
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2096
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2161
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2246
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2321
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2490
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..2581
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2582..2707
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     exon            2708..9415
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3040..3045
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /note="hexamer: AGTAAA"
     polyA_site      3069
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /note="major polyA site"
     regulatory      5818..5823
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /note="hexamer: AATAAA"
     polyA_site      5840
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
     regulatory      9391..9396
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
                     /note="hexamer: AATAAA"
     polyA_site      9415
                     /gene="PIK3C3"
                     /gene_synonym="hVps34; Vps34; VPS34"
ORIGIN      
        1 atgttgtttt tcctgtacct aagttcccgc tgtaggtggt acctttgcag acggtgcgat
       61 gggggaagca gagaagtttc actacatcta tagttgtgac ctggatatca acgtccagct
      121 taagatagga agcttggaag ggaagagaga acaaaagagt tataaagctg tcctggaaga
      181 cccaatgttg aagttctcag gactatatca agagacatgc tctgatcttt atgttacttg
      241 tcaagttttt gcagaaggga agcctttggc cttgccagtg agaacatcct acaaagcatt
      301 tagtacaaga tggaactgga atgaatggct gaaactacca gtaaaatacc ctgacctgcc
      361 caggaatgcc caagtggccc tcaccatatg ggatgtgtat ggtcccggaa aagcagtgcc
      421 tgtaggagga acaacggttt cgctctttgg aaaatacggc atgtttcgcc aagggatgca
      481 tgacttgaaa gtctggccta atgtagaagc agatggatca gaacccacaa aaactcctgg
      541 cagaacaagt agcactctct cagaagatca gatgagccgt cttgccaagc tcaccaaagc
      601 tcatcgacaa ggacacatgg tgaaagtaga ttggctggat agattgacat ttagagaaat
      661 agaaatgata aatgagagtg aaaaacgaag ttctaatttc atgtacctga tggttgaatt
      721 tcgatgtgtc aagtgtgatg ataaggaata tggtattgtt tattatgaaa aggacggtga
      781 tgaatcatct ccaattttaa caagttttga attagtgaaa gttcctgacc cccagatgtc
      841 tatggagaat ttagttgaga gcaaacacca caagcttgcc cggagtttaa gaagtggacc
      901 ttctgaccac gatctgaaac ccaatgctgc cacgagagat cagttaaata ttattgtgag
      961 ttatccacca accaagcaac ttacatatga agaacaagat cttgtttgga agtttagata
     1021 ttatcttacg aatcaagaaa aagccttgac aaaattcttg aaatgtgtta attgggatct
     1081 acctcaagag gccaaacagg ccttggaact tctgggaaaa tggaagccga tggatgtaga
     1141 ggactccttg gagctgttat cctctcatta caccaaccca actgtgaggc gttatgctgt
     1201 tgcccggttg cgacaggccg atgatgagga tttgttgatg tacctattac aattggtcca
     1261 ggctctcaaa tatgaaaatt ttgatgatat aaagaatgga ttggaaccta ccaagaagga
     1321 tagtcagagt tcagtgtcag aaaatgtgtc aaattctgga ataaattctg cagaaataga
     1381 tagctcccaa attataacca gcccccttcc ttcagtctct tcacctcctc ctgcatcaaa
     1441 aacaaaagaa gttccagatg gcgaaaatct ggaacaagat ctctgtacct tcttgatatc
     1501 gagagcctgc aaaaactcaa cactggctaa ttatttatac tggtatgtga tagtggaatg
     1561 tgaagatcaa gatactcagc agagagatcc aaagacccat gagatgtact tgaacgtaat
     1621 gagaagattc agccaagcat tgttgaaggg tgataagtct gtcagagtta tgcgttcttt
     1681 gctggctgca caacagacat ttgtagatcg gttggtgcat ctaatgaagg cagtacaacg
     1741 cgaaagtgga aatcgtaaga aaaagaatga gagactacag gcattgcttg gagataatga
     1801 aaagatgaat ttgtcagatg tggaacttat cccgttgcct ttagaacccc aagtgaaaat
     1861 tagaggaata attccggaaa cagctacact gtttaaaagt gcccttatgc ctgcacagtt
     1921 gttttttaag acggaagatg gaggcaaata tccagttata tttaagcatg gagatgattt
     1981 acgtcaagat caacttattc ttcaaatcat ttcactcatg gacaagctgt tacggaaaga
     2041 aaatctggac ttgaaattga caccttataa ggtgttagcc accagtacaa aacatggctt
     2101 catgcagttt atccagtcag ttcctgtggc tgaagttctt gatacagagg gaagcattca
     2161 gaactttttt agaaaatatg caccaagtga gaatgggcca aatgggatta gtgctgaggt
     2221 catggacact tacgttaaaa gctgtgctgg atattgcgtg atcacctata tacttggagt
     2281 tggagacagg cacctggata accttttgct aacaaaaaca ggcaaactct tccacataga
     2341 ctttggatat attttgggtc gggatccaaa gcctcttcct ccaccaatga agctgaataa
     2401 agaaatggta gaaggaatgg ggggcacaca gagtgagcag taccaagagt tccgtaaaca
     2461 gtgttacacg gctttcctcc acctgcgaag gtattctaat ctgattttga acttgttttc
     2521 cttgatggtt gatgcaaaca ttccagatat tgcacttgaa ccagataaaa ctgtgaaaaa
     2581 ggttcaggat aaattccgct tagacctgtc ggatgaagag gctgtgcatt acatgcagag
     2641 tctgattgat gagagtgtcc atgctctttt tgctgcagtg gtggaacaga ttcacaagtt
     2701 tgcccagtac tggagaaaat gaaactggga ttgacccatc aagatgcttg gctcaataag
     2761 aaaaccacgt taggagcaac ctttgtatat tggagacttc agagtaacca gcaaggaaga
     2821 gaaatcttaa tcttcaagtt accatatttt ccaaatatta catggtacct gagttctgct
     2881 tccttggatg tcattgctta aatatagtct tgaagggctt gttttgaaat attgtatata
     2941 ttttttcaaa tgtatacatt gttaataaat taagaaatga gaaacattct tatttatgta
     3001 catgttatga gagttctgga ggaagtaata tgttgaaata gtaaaacatt ttagtttttg
     3061 agtttaaaat gttatttttt cctcttatat cttcatatca ggacagcagt aacctgaatt
     3121 tagattgtca cagtttacaa gtttttgttt gttccttctt tcctctctct tctcccactc
     3181 acttttgtgt gttgtttgtt tttgcctaat ttacatgttt aaagttctac ttaaaatttt
     3241 tcttcaagtt ttacatgtga tggctcattc ttctgcagtt caagaattgc ttatattttt
     3301 agaagatact cattttctct tacaaataat taattttttg taaactacag ttaatagttc
     3361 ttctagcttg taagtgtgta aagtagaata tgaaaatgtg gctatttaga taactggcca
     3421 aaaggtcata tgaccaccag ttttagtatt gagcataaga tatttttaca tattcatgta
     3481 agacaatgta aattgtcttt ctgcaataaa ttttttggat gcaaatttag atttcaaaag
     3541 ctttttgatg ttataaagga gttgaggaac aaaaagctct ttgaaatagc tcacctagac
     3601 ctgttaatgt aatatattct ttctgaatta agcaaaattt tttttgtctt ttaagactag
     3661 aactaatccc tctagctttt ttactggaac tttggggaaa aagaacaaat acaaatatac
     3721 aaatgatatg aacaatatgg taataatctt ttgcaactta atatgttaca tagtcatatg
     3781 acttggtctt ggaagaaaaa gaattgtttt ggtgctgaat aaatattaga aatcaagtga
     3841 cagagaagat gaaggtaaaa ttgtggcaca gtttcagaga atgccgttaa tgataacgtg
     3901 ttatttgtct caaattctta gccctgggct tgcctcttca gagtggggtt accggcgaga
     3961 gggcaagcat gtaattaaga gacactctag aggcatatgg aatatagttt gtataaaaca
     4021 tacctgctga ttagaaaaat ggtaaaaatg aaaatacctt tgtacatcat cttaagagag
     4081 cttatatata aactaaatgg gcaggctcca tcgtctacct ttctttttct tttcttcaca
     4141 tgtttatgtc ttttttgtta ttagtcagac atttttagcc ctctgtatgc tcattcttgt
     4201 agttctattt ctattgaaac ttcctccaaa acttttctct gaattaccct tgttgaaact
     4261 gacctttctt ccgtgtgaac tcctacactt gcatctccta tttcaggatt tggcatactt
     4321 tttctttaat gggctaagaa agtaaatatc ttcagctttg gggggcatat gatcccaata
     4381 gcatcaactt aactctgcta actggaaggc aaaagtgtat catcttactc gtctttataa
     4441 actccacagc actctgcata gatatgacca tgtttataag gaactcaata aataattttg
     4501 acacaaacat gaaattctag aagggcagac actgacaact agtaaggaag ttgatcatga
     4561 gtaactttgt attcagtgcc accttaacaa gaattgtaaa gaggataaac ctttctagga
     4621 aggttgatgc gtgttgggtg aggaaggagc atctctcagg agggtaaaca gtagctgtgg
     4681 ttttgctgag agattttggc tgtgagtgac aagcctatat caaaaagaaa agtggcttat
     4741 caatctcagt ttttattgct caaagcaatt ctttactcct attttttcca aattccattg
     4801 tttttactct ttagagctct atcctcagag tgtagtctgc atatttcttt attatcattg
     4861 ccatgtgact tctttggtga caagttcagt gatatttcaa gcatgatgtt ttgagtttga
     4921 gaaatgtatt tgtgaggaca catcacttta attaaaaaag tgtgagtact atggtcatta
     4981 agttattcag agtgagattt ctgagaggag aagagagaat gtagatggca gttattgaga
     5041 gtggggctat aggacaaaaa gaagaaaagt atggttaaca ttgccaaata aagggtcata
     5101 cagccttgct ttttgctgga gttagcaaaa atcattctcc atatgatagt tttagatgtt
     5161 ccccagctca tttgtattat tgagttttca gctgggtata tattataata acagtcaaca
     5221 tgatgtaggg gctcagtatt acatggcagg gctatactaa ttgcttcata tgccttatct
     5281 cattattctg tgagatagaa ttgtctcctc attttttagt tgaaataact gaagtttaaa
     5341 gcaattgaat catctgccca aggataagct gctggtgaga gcagagttgg gatttgaagt
     5401 cgagttagac cccagtgatc acagtcttga cgattaaatt cttccagctt tcatttttca
     5461 ctgagataat ggtagtgata gtactgacct ctaatgtgtg catttgtggg tatgtggtcc
     5521 attcagcttt aatccccaga agacaaggct tattcctttt cttatttttg gtcatgtttt
     5581 atttttccat tgcttttaac aggattacca aaggcacact cagtagtcag taaacacatt
     5641 tctaggaaag gtgttgtgtc atcatgccac atattcatac tttccttggg ttggaaaata
     5701 gatcattcag taaaaacata caggaaaaat gaatcttgcc aatgcaattg ttaacctaca
     5761 accataatat accttaagta tatttttgca cataagtata acattgcgat ttaaaacaat
     5821 aaaccagatt gagattctaa ggagcatttt gtaagtaatt actaatgttt attttagaga
     5881 gatcacacaa cttcaaataa aaactgacat agattgaaca ccttgagaat aaactttagt
     5941 gccaaatgga aaataatttt ttacaagtaa atttgaagaa caatgtgaac tttctataat
     6001 tatatacaga aaatatactg atttgccaaa atgagtaatt ttgatatatt aatatttcac
     6061 ttataagaat gcataccacc tgatccagga tgggatccag gaacagaaaa agaacattag
     6121 gtaaaaatga cagaaatctg aatatagtat agagtagcta aaaacaaacc aaaaaaaaaa
     6181 aaaaaaaaaa aggaaaaaca gaataagcct cagaagatag tgagcatccc acctgggtga
     6241 ccacttagag aagctgaggt gggacccctg catccgataa gtggctgcac tagaccacag
     6301 gaaccagact ccactgtaag aactagagtg ggaatggaca tgtcaaaacc tcctgggaac
     6361 cttattaaat gtgcacattc ccaatcccca ctcctgagca gccaaatcaa tctgcaggtg
     6421 ggccttgtga gttttcccag gggattccga tgcctgctag atttaaccag tagatgagat
     6481 gttaagttat cctgtagtcc agagattcca caggccagtg tcagggaaca ggggccaggg
     6541 ctgggaaggc ataagaacag aagataaatt tgaggctctg ttacgctggt catccaaccc
     6601 aaccaggtat ctcttgttat atgctaactt cattttccct ttcttgtcca tagtgggaaa
     6661 aaataatttt taactggcta tgtagttaaa aaacctaaca aaaagcactt ttctatataa
     6721 tgagtggttt atattaagaa taggtacaat gtaaatactg gtaagtgaaa gttcattttg
     6781 ttcagtgcta tgggctggta cattgcattt caacactagt tttttacttt tatctaccct
     6841 gagggtagat aacccatatt ccaaaataca gtattatttt catttgctgt aacattttaa
     6901 gaaatatgta gtttctaaaa cttcatatac tttatatgga atatgaacaa ctttaatatg
     6961 aataaatagt ctagaaaaaa atttaggaaa aattgccatg aaatcatgag ctaagtcaat
     7021 ggttatttta aaccacttaa ttttgagact agtatttcat acagaaaaag ctaatactga
     7081 tatcacacac taattgtgta taaaacacta ggaatgtcaa aatagaacat aagaaaatga
     7141 gtctagtgga cccactgcat agtgtaactt tgggatgaat ttaaaagaat atttaagtca
     7201 cagtcaattt tctgttggtc taattttacc aaataacccc ctaaataaga agtctgattt
     7261 atctaaaata gaagttaaca tagcatccca gtattgccat tctaaaactc tggcatgtaa
     7321 atgggagaaa tggaaataag ctcaaaatgg gtagagtaac ccatttctgc atgaaccata
     7381 gaatttaact agaatttggc tggctcatgc ctgtaatgcc agcactttgg gaggccaagg
     7441 caggcagatc acttgagccc aggagtttga gaccagcccg ggcaacatgg tgaaactcca
     7501 tctcttcaaa aatatatatt tgtgtgtgtg tgtgtgtgta tgtgcgtgca caaaaattag
     7561 ctgagtgtgg tggttcacac ttgtaatcac agctacctgg gaggctgagg caggaggatt
     7621 gcttgagcct gggaggcaga ggttgcagtg agctgagatt gcaccattgc actgcagcct
     7681 gggtaataga gcgagacaac tctatctcaa aaaaaagaat ttcactagaa tttatatctt
     7741 cagtgtttgc attagattgt caccagcact atctgccttg tgactgtcag gactaatcct
     7801 tatatcattt atcttatgtc ctttacctca gtttatgtct gtcaccacaa gaaacccaga
     7861 cctccctaat cataccatat gccctggtct tacccacata cagctcggta caactgcatt
     7921 tccccaaacc tcagaactcc ttgggccatc actgtcagag acatttgaac tagagcaact
     7981 ccatcttgag tagggactgg gtataatgaa gctgagaccc actgggctgc attcccagga
     8041 agttaggcat tcttagtcac aggatgagat aggagattgg ctcaagataa ggtcacaaag
     8101 accttgctga taaaacagtc tgtggtaaag aagccagcca aaacccacca aaaccaagac
     8161 gggaatgaaa gtgacctccg gtcatcctca ctgctcatta tacgctagtt ataatgcatt
     8221 gacatgctaa gagacactcc caccagcacc atgacagttt acaaatgcca taacaacatc
     8281 aggaagttac cctatatggt ctaaaaaggg gaggaaccct cagttctgag aatttccttg
     8341 aaaattcatg aataatccac cccttgttta gcatatgatc aagaaataat cattaaaaaa
     8401 tagccaacca gcagctcatg ctgctggtct gcctatggag tagccatcct ttctttatta
     8461 acctgctttc actttatgga ctcccctgaa ttcttctgca ggagatccaa gaaccctctc
     8521 ttggagtctt aaccaggatc cctttccagt aacttcagca ggatctgtga gtgccagttt
     8581 ccttctgtgg tgcaaaacta ggtgctaagt acactttttc ccccctttcc tctattttta
     8641 aaagaacttt cagccctttt gaagtgggta cattgtctgg agtacatacc ctggggttca
     8701 ttgttatagg agaatttagg acacagacac acgaggagtt taggagcaga ggtttaatag
     8761 gtagaagaga agggaaagag aaacagcttc ctctatagat gtctgcactg acaccgtggc
     8821 tttaaggcat ggcccatgat gtgtctccga gtggaaaaga ctggttggtg gtgaatgcgc
     8881 caaattttat agtccggttt gaggaggtgg tttctgattg gttgcatcat gtatgacgtt
     8941 tgcaaggtgc atgtggaagg ctggtcgccc caccctaatc ttatgcaaat gggctttcca
     9001 gttgattggg tccatcttgt gtaacacgtg gctggcagag aagggaagat gaagccacca
     9061 tcttgaaaat gtctagtcct tagtttctgc tggcattcac ccatgcaagc tgccagcttg
     9121 tctatgtctg cagctcaact ttacaggctg ctctttgtta gcaaatgatt tggagcttct
     9181 tttcattaaa gaaaaaagcc ttaccgagga ctcccatacc cttgctttct gcttaagtga
     9241 tttcttctta actcctatgt caattttata cagcatgaaa taatgatgta tctataaatg
     9301 agcctttgca agattaccat ttatcaaaga aaaccagtga tgtttagtta actgtttgta
     9361 aaaataaatg gttagtagaa tgtctaattc aataaatgtt tcataaatga gtaaa
//
