LOCUS       NM_001377334            7658 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens phosphatidylinositol-4-phosphate 3-kinase catalytic
            subunit type 2 beta (PIK3C2B), transcript variant 1, mRNA.
ACCESSION   NM_001377334 XM_005245257
VERSION     NM_001377334.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Gozzelino,L., Kochlamazashvili,G., Baldassari,S., Mackintosh,A.I.,
            Licchetta,L., Iovino,E., Liu,Y.C., Bennett,C.A., Bennett,M.F.,
            Damiano,J.A., Zsurka,G., Marconi,C., Giangregorio,T., Magini,P.,
            Kuijpers,M., Maritzen,T., Norata,G.D., Baulac,S., Canafoglia,L.,
            Seri,M., Tinuper,P., Scheffer,I.E., Bahlo,M., Berkovic,S.F.,
            Hildebrand,M.S., Kunz,W.S., Giordano,L., Bisulli,F., Martini,M.,
            Haucke,V., Hirsch,E. and Pippucci,T.
  TITLE     Defective lipid signalling caused by mutations in PIK3C2B underlies
            focal epilepsy
  JOURNAL   Brain 145 (7), 2313-2331 (2022)
   PUBMED   35786744
  REMARK    GeneRIF: Defective lipid signalling caused by mutations in PIK3C2B
            underlies focal epilepsy.
REFERENCE   3  (bases 1 to 7658)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 7658)
  AUTHORS   Koch,P.A., Dornan,G.L., Hessenberger,M. and Haucke,V.
  TITLE     The molecular mechanisms mediating class II PI 3-kinase function in
            cell physiology
  JOURNAL   FEBS J 288 (24), 7025-7042 (2021)
   PUBMED   33387369
  REMARK    GeneRIF: The molecular mechanisms mediating class II PI 3-kinase
            function in cell physiology.
            Review article
REFERENCE   5  (bases 1 to 7658)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 7658)
  AUTHORS   Ueno,H., Honda,H., Nakamoto,T., Yamagata,T., Sasaki,K.,
            Miyagawa,K., Mitani,K., Yazaki,Y. and Hirai,H.
  TITLE     The phosphatidylinositol 3' kinase pathway is required for the
            survival signal of leukocyte tyrosine kinase
  JOURNAL   Oncogene 14 (25), 3067-3072 (1997)
   PUBMED   9223670
REFERENCE   7  (bases 1 to 7658)
  AUTHORS   Brown,R.A., Ho,L.K., Weber-Hall,S.J., Shipley,J.M. and Fry,M.J.
  TITLE     Identification and cDNA cloning of a novel mammalian C2
            domain-containing phosphoinositide 3-kinase, HsC2-PI3K
  JOURNAL   Biochem Biophys Res Commun 233 (2), 537-544 (1997)
   PUBMED   9144573
REFERENCE   8  (bases 1 to 7658)
  AUTHORS   Milani,D., Mazzoni,M., Borgatti,P., Zauli,G., Cantley,L. and
            Capitani,S.
  TITLE     Extracellular human immunodeficiency virus type-1 Tat protein
            activates phosphatidylinositol 3-kinase in PC12 neuronal cells
  JOURNAL   J Biol Chem 271 (38), 22961-22964 (1996)
   PUBMED   8798481
REFERENCE   9  (bases 1 to 7658)
  AUTHORS   Lanfranchi,G., Muraro,T., Caldara,F., Pacchioni,B., Pallavicini,A.,
            Pandolfo,D., Toppo,S., Trevisan,S., Scarso,S. and Valle,G.
  TITLE     Identification of 4370 expressed sequence tags from a
            3'-end-specific cDNA library of human skeletal muscle by DNA
            sequencing and filter hybridization
  JOURNAL   Genome Res 6 (1), 35-42 (1996)
   PUBMED   8681137
REFERENCE   10 (bases 1 to 7658)
  AUTHORS   Kozutsumi,H., Toyoshima,H., Hagiwara,K., Yazaki,Y. and Hirai,H.
  TITLE     Human ltk receptor tyrosine kinase binds to PLC-gamma 1, PI3-K, GAP
            and Raf-1 in vivo
  JOURNAL   Oncogene 9 (10), 2991-2998 (1994)
   PUBMED   8084603
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL606489.26.
            
            On Jan 7, 2020 this sequence version replaced XM_005245257.2.
            
            Summary: The protein encoded by this gene belongs to the
            phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in
            signaling pathways involved in cell proliferation, oncogenic
            transformation, cell survival, cell migration, and intracellular
            protein trafficking. This protein contains a lipid kinase catalytic
            domain as well as a C-terminal C2 domain, a characteristic of class
            II PI3-kinases. C2 domains act as calcium-dependent phospholipid
            binding motifs that mediate translocation of proteins to membranes,
            and may also mediate protein-protein interactions. The PI3-kinase
            activity of this protein is sensitive to low nanomolar levels of
            the inhibitor wortmanin. The C2 domain of this protein was shown to
            bind phospholipids but not Ca2+, which suggests that this enzyme
            may function in a calcium-independent manner. [provided by RefSeq,
            Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB210006.1, Y13892.1 [ECO:0000331]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000684373.1/ ENSP00000507222.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression, longest
                                         protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-450               AL606489.26        146314-146763       c
            451-1467            AL606489.26        120828-121844       c
            1468-1568           AL606489.26        117177-117277       c
            1569-1723           AL606489.26        116408-116562       c
            1724-1844           AL606489.26        115970-116090       c
            1845-1956           AL606489.26        112508-112619       c
            1957-2036           AL606489.26        112282-112361       c
            2037-2100           AL606489.26        111836-111899       c
            2101-2247           AL606489.26        109686-109832       c
            2248-2281           AL606489.26        108995-109028       c
            2282-2477           AL606489.26        107814-108009       c
            2478-2600           AL606489.26        106627-106749       c
            2601-2768           AL606489.26        101808-101975       c
            2769-2880           AL606489.26        101143-101254       c
            2881-3023           AL606489.26        99394-99536         c
            3024-3212           AL606489.26        97914-98102         c
            3213-3306           AL606489.26        96289-96382         c
            3307-3401           AL606489.26        96026-96120         c
            3402-3582           AL606489.26        95375-95555         c
            3583-3690           AL606489.26        94484-94591         c
            3691-3783           AL606489.26        93429-93521         c
            3784-3913           AL606489.26        92150-92279         c
            3914-4050           AL606489.26        90893-91029         c
            4051-4220           AL606489.26        86397-86566         c
            4221-4377           AL606489.26        85751-85907         c
            4378-4487           AL606489.26        85274-85383         c
            4488-4689           AL606489.26        84158-84359         c
            4690-4814           AL606489.26        83627-83751         c
            4815-4932           AL606489.26        81879-81996         c
            4933-5014           AL606489.26        80097-80178         c
            5015-5121           AL606489.26        79606-79712         c
            5122-5250           AL606489.26        77571-77699         c
            5251-7658           AL606489.26        74591-76998         c
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..7658
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /note="phosphatidylinositol-4-phosphate 3-kinase catalytic
                     subunit type 2 beta"
                     /db_xref="GeneID:5287"
                     /db_xref="HGNC:HGNC:8972"
                     /db_xref="MIM:602838"
     exon            1..450
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            451..1467
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     CDS             535..5439
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /EC_number="2.7.1.137"
                     /EC_number="2.7.1.154"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphatidylinositol 3-kinase C2 domain-containing beta
                     polypeptide; PTDINS-3-kinase C2 beta; PI3K-C2beta;
                     PI3K-C2-beta; ptdIns-3-kinase C2 subunit beta;
                     phosphoinositide 3-kinase-C2-beta; phosphatidylinositol
                     4-phosphate 3-kinase C2 domain-containing subunit beta;
                     phosphoinositide-3-kinase, class 2, beta polypeptide"
                     /codon_start=1
                     /product="phosphatidylinositol 4-phosphate 3-kinase C2
                     domain-containing subunit beta isoform 1"
                     /protein_id="NP_001364263.1"
                     /db_xref="CCDS:CCDS1446.1"
                     /db_xref="GeneID:5287"
                     /db_xref="HGNC:HGNC:8972"
                     /db_xref="MIM:602838"
                     /translation="MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDK
                     EENRAKQNADPSLISWDEPGVDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGP
                     PNHSTSQGPQPGSDPWPKGSLSGDYLYIFDGSDGGVSSSPGPGDIEGSCKKLSPPPLP
                     PRASIWDTPPLPPRKGSPSSSKISQPSDINTFSLVEQLPGKLLEHRILEEEEVLGGGG
                     QGRLLGSVDYDGINDAITRLNLKSTYDAEMLRDATRGWKEGRGPLDFSKDTSGKPVAR
                     SKTMPPQVPPRTYASRYGNRKNATPGKNRRISAAPVGSRPHTVANGHELFEVSEERDE
                     EVAAFCHMLDILRSGSDIQDYFLTGYVWSAVTPSPEHLGDEVNLKVTVLCDRLQEALT
                     FTCNCSSTVDLLIYQTLCYTHDDLRNVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYC
                     RKFDIDIRLQLMEQKVVRSDLARTVNDDQSPSTLNYLVHLQERPVKQTISRQALSLLF
                     DTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQ
                     PKIQKDPSVLAVRENREKVVEALTAAILDLVELYCNTFNADFQTAVPGSRKHDLVQEA
                     CHFARSLAFTVYATHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFH
                     LIVWDQQICFPVQVNRLPRETLLCATLYALPIPPPGSSSEANKQRRVPEALGWVTTPL
                     FNFRQVLTCGRKLLGLWPATQENPSARWSAPNFHQPDSVILQIDFPTSAFDIKFTSPP
                     GDKFSPRYEFGSLREEDQRKLKDIMQKESLYWLTDADKKRLWEKRYYCHSEVSSLPLV
                     LASAPSWEWACLPDIYVLLKQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAE
                     LLDYLPQLVQALKYECYLDSPLVRFLLKRAVSDLRVTHYFFWLLKDGLKDSQFSIRYQ
                     YLLAALLCCCGKGLREEFNRQCWLVNALAKLAQQVREAAPSARQGILRTGLEEVKQFF
                     ALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLSFQNVDPLGENIRVIFKCGDDL
                     RQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGRGMVEMIPNAETLRKIQVEHG
                     VTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYVLGICDRHNDNIMLK
                     TTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCC
                     QAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLI
                     ESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFASRTHTLKSSGRISDVFLCRHEKI
                     FHPNKGYIYVVKVMRENTHEATYIQRTFEEFQELHNKLRLLFPSSHLPSFPSRFVIGR
                     SRGEAVAERRREELNGYIWHLIHAPPEVAECDLVYTFFHPLPRDEKAMGTSPAPKSSD
                     GTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQLLQDGNDPDPYVKIYLLPDPQK
                     TTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENVLLGEVNIR
                     LRELDLAQEKTGWFALGSRSHGTL"
     exon            1468..1568
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1723
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1844
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1956
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2036
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2100
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2247
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2281
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2477
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2478..2600
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2768
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2880
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            2881..3023
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3024..3212
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3213..3306
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3307..3401
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3402..3582
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3583..3690
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3691..3783
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3784..3913
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            3914..4050
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            4051..4220
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            4221..4377
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            4378..4487
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            4488..4689
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            4690..4814
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            4815..4932
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            4933..5014
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            5015..5121
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            5122..5250
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     exon            5251..7658
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7635..7640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /note="hexamer: AATAAA"
     polyA_site      7658
                     /gene="PIK3C2B"
                     /gene_synonym="C2-PI3K"
                     /note="major polyA site"
ORIGIN      
        1 ggattcggcg ctgcggctgg agccggcggc cgggtgcctg cctgtccagg cagggtctgg
       61 ccgagcgtcg cgtcgccgtg cagtgcgccg ggtgtccggg agatggcttc ccctctctcg
      121 cccgctgctg ccgctattgt ctcacccggg gtggcctgac tctgcgagct cgcgcacgct
      181 ctcggcggag agcacccctc cccaccccca caagctggtc cttgcgccct ttgctgcctc
      241 tccctctgcc ccttggagcc tagggtctgt gcggagcctg gagaggggag cggcccggga
      301 gcaggcggcg tgcggccggc cagctcctct cgcgctggtg caagaggcgc ctggagccgc
      361 agagcgagcc cgggaggcgc ggagcgagca gcccacgtcg ccaggcacaa gacacgtcgg
      421 atgttgcagc agagccagca gctagcgcag gcgcccagac accatgtcac aaccctccag
      481 aactgacgtt ggcaggaagt agagactttg ttgcctgtgt cccccatcct caccatgtct
      541 tcgactcagg gcaatgggga acactggaag tccctggagt cagtgggcat cagccgcaaa
      601 gagctagcga tggccgaagc cctgcagatg gagtatgatg ccctgtcccg gctccggcat
      661 gacaaggagg agaacagagc caagcagaac gcagacccct ctctcatcag ctgggatgag
      721 cctggggtag acttttacag caagccagca ggaaggcgga ccgacctcaa gctgttacgc
      781 ggtctctctg gctctgatcc tacccttaac tacaactcac tctccccaca ggaagggccg
      841 cccaaccact ctacctccca agggccacag cctggctcag atccctggcc caaaggctcc
      901 ctgtctggag actatctcta catttttgat ggttcagatg ggggagtctc ttcgtcccca
      961 ggaccagggg acatagaggg ctcttgcaag aaactatccc cacctcctct gcctccccga
     1021 gcttctatct gggatacccc tcccctgcct cccagaaagg ggtccccctc atcctccaag
     1081 atctcccagc ccagtgacat caacactttc tctttggtcg aacaattgcc gggcaaactg
     1141 ctagagcatc ggatcctaga agaggaagag gtgctgggag gtgggggtca ggggcgccta
     1201 ctggggtctg tggactatga tggtatcaat gatgcaatta ctaggctcaa cttgaaatcg
     1261 acctatgatg cggagatgtt gcgggatgcc accaggggct ggaaggaggg ccgagggccg
     1321 ctggacttca gcaaagacac ctctggaaaa cccgtggcca ggagcaagac tatgccccct
     1381 caggtgcccc cccgcaccta tgcctcccgc tatggcaacc gaaagaatgc gacgcctggc
     1441 aagaaccgcc ggatttctgc agccccggtg ggctcccggc cccacactgt tgccaatggc
     1501 catgagttgt ttgaggtctc agaagagaga gatgaggagg ttgctgcatt ttgccacatg
     1561 ctggatatcc ttcgatctgg ctctgacatc caagactact tcctcactgg ctatgtctgg
     1621 agtgctgtca cccctagccc agagcacctc ggggatgagg tcaacctgaa ggtgactgtg
     1681 ttgtgtgaca ggcttcaaga ggcactcact ttcacctgca actgttcctc cactgtagac
     1741 ttgcttatct accagaccct gtgctacacc catgatgacc tgaggaatgt ggacgtgggt
     1801 gactttgtgc taaagccctg cgggctggag gagttcctgc agaacaagca tgccttgggc
     1861 agtcatgagt acatccaata ctgccgcaag tttgacattg acattcggct acagctgatg
     1921 gagcagaagg ttgtgcgcag tgacctggcc cggacggtga atgatgacca gagcccctcc
     1981 accttgaact acctcgtcca tctccaagag aggcctgtca agcagaccat cagcaggcag
     2041 gccctgagtc ttctgttcga cacttaccac aatgaggtgg atgccttcct gctggctgat
     2101 ggagacttcc cactgaaggc tgacagggtg gtccagtccg tcaaggccat ctgcaacgcc
     2161 ctggccgccg tggaaacccc tgagatcacc agtgctctca accagctgcc cccctgcccc
     2221 tcccgcatgc agcctaaaat tcagaaggat cccagtgtct tggctgtgag ggaaaaccga
     2281 gagaaggtcg tggaagccct gaccgctgcc atcttggacc tggtggagct gtactgcaac
     2341 acattcaacg cagacttcca gacggcagtg cccgggagcc gcaagcatga cctggtccag
     2401 gaggcctgcc atttcgccag gtccctggcc ttcactgtct atgccaccca ccgcatcccc
     2461 atcatctggg ctaccagcta tgaagatttc tacctctcct gctccctcag ccatggcggc
     2521 aaggagctgt gcagccccct gcagacccga agagctcact tctccaagta cctcttccac
     2581 ctcatcgtct gggaccagca gatctgcttc ccagtgcagg tgaaccggct gcctcgggag
     2641 acactgctgt gtgccactct ctatgctctg cccatccccc caccggggag ctcctcagag
     2701 gccaataagc agcggcgggt gcctgaagcc ctgggctggg tcactacccc actcttcaac
     2761 ttcaggcagg tcctgacctg tggccggaag cttctgggtt tgtggccagc aacacaggaa
     2821 aatcccagcg cccgttggag tgcacctaat ttccaccagc cagacagtgt catcctgcag
     2881 attgacttcc ccacctcggc ctttgacatc aagttcacca gcccccctgg agacaagttc
     2941 agcccccgct atgagtttgg cagcctccgg gaagaagacc agcgcaagct taaagacatc
     3001 atgcagaaag agtccttgta ctggctcact gatgctgaca agaagcgcct gtgggagaag
     3061 cgatattact gccactcgga ggtgagctcg ctccccctgg tgctcgccag cgcccccagc
     3121 tgggagtggg cttgcctgcc tgacatctat gttctcctga agcagtggac ccacatgaac
     3181 caccaggatg ccctggggct cctgcatgcc accttcccgg accaggaggt gcgtcgtatg
     3241 gctgtgcagt ggattggctc actctcagat gctgagctgc tagactacct gccccagctg
     3301 gtacaggccc tgaagtatga atgctacctg gacagcccgt tggtgcgctt cctcctgaaa
     3361 cgagctgtgt ctgacttgag agtgactcac tacttcttct ggttactgaa ggacggcctc
     3421 aaggactctc agttcagcat ccgctaccag tatctgctgg cagccttact gtgctgctgt
     3481 ggcaaggggc tgagagaaga gtttaaccgc cagtgctggc ttgtcaatgc cctggccaaa
     3541 ctggcccagc aggtccggga ggcagcccca tctgcaaggc agggaatcct ccgcacgggc
     3601 ctggaggagg tgaagcagtt ctttgccctc aatggctcgt gccgcttgcc actcagcccc
     3661 agtctgctgg ttaagggaat tgtgcccagg gactgttcct acttcaactc caatgctgtc
     3721 cccctcaaac tctccttcca aaatgtggat cccctgggtg agaacatccg tgtcatcttc
     3781 aagtgtgggg acgaccttcg ccaggacatg ctaacgctgc agatgattcg catcatgagc
     3841 aagatctggg tccaggaggg gctggacatg cgcatggtca tcttccgctg cttctccacc
     3901 ggccggggca gagggatggt ggagatgatc cctaatgctg agaccctgcg taagatccag
     3961 gtggagcatg gggtgaccgg ctcgttcaag gaccggcccc tggcagactg gctgcagaaa
     4021 cacaaccctg gggaggacga gtatgagaag gctgtggaga actttatcta ctcctgcgct
     4081 ggctgctgcg tggccacgta cgtcttgggc atctgtgacc gacataatga caacatcatg
     4141 ctgaagacca ctggtcacat gttccacatt gattttggcc gcttcctggg ccatgcccag
     4201 atgtttggca acatcaagcg ggaccgtgcc ccctttgtct tcacctcgga catggcgtat
     4261 gtcatcaacg ggggtgacaa gccttccagc cgcttccatg attttgttga cctttgctgc
     4321 caagcctaca acctcattcg caagcacacc cacctcttcc tcaaccttct gggcctgatg
     4381 ttgtcctgtg ggatccctga actctcagac ctggaggacc tcaagtatgt gtacgatgcc
     4441 ctgaggcctc aggatacaga ggccaatgcc actacctact tcactaggtt gattgagtcc
     4501 agcctgggca gtgtagccac aaagctcaat tttttcatcc ataatctggc tcagatgaag
     4561 ttcacgggct cagatgaccg gctgaccctc tcctttgcct cccgaacaca cactctcaag
     4621 agctctggcc gaatcagtga tgttttcctc tgccgccatg agaagatctt ccaccccaac
     4681 aaaggctata tatatgtggt aaaggtgatg cgagagaaca ctcacgaggc cacctacatc
     4741 cagcggacct ttgaggagtt ccaggaatta cacaataagt tgcggctgct cttcccttct
     4801 tcccacttgc ccagcttccc tagtcgcttc gtgatcggcc gctcccgggg agaggcggtg
     4861 gccgagcggc ggagggagga gctaaacggt tacatctggc acttgatcca cgcaccccct
     4921 gaggtggccg agtgtgattt ggtgtacacc ttcttccacc cactgccccg ggatgagaag
     4981 gctatgggca ccagcccagc tcctaagtcc tcagatggca catgggcccg gcccgtcgga
     5041 aaggtgggag gggaggtgaa gctgtccatc tcctacaaaa acaataaact cttcatcatg
     5101 gtgatgcata ttcggggctt gcaactgctc caggatggaa atgaccctga cccctatgtg
     5161 aaaatttacc tccttcctga ccctcagaaa accactaaga ggaaaaccaa agtggcccgg
     5221 aaaacctgca atcctaccta caatgagatg ttggtatatg atgggatccc caagggtgac
     5281 ctgcagcagc gggagctcca gctgagcgtg ctgagtgagc agggattctg ggagaacgtc
     5341 ctcctcggtg aggtgaacat ccgcctgcga gagctggacc tggctcagga gaagaccggc
     5401 tggttcgccc tgggatctcg aagtcatggc accttgtgag cccagcagag ccaccaccca
     5461 gcatcccagg ctggtggcag gagctggggg agaggactct cccctgtgag actcctcctt
     5521 gtgaagggcc agggccctgg gcaggcctcc agctcggtcc aggtgattct ggcctctgtg
     5581 gtaggaggca gggagagtaa gacatgctct gctgtctctt cctctggaga ctgaacttgg
     5641 gttggttgtg atgagcagcc ccttggaggc tgtgaggttg cagcaaagtt ttaagtttac
     5701 cttgtgtcaa gggagcaatg cttggtttgg ggagtgtgtg gggtgggctg tatgaagtag
     5761 cattttgggg gtgggtgggt ggatatctta atttttattt ttaaaaaatg aaatagtgat
     5821 gttgtcctaa ctgggacagg aagccttgcg agaagggacg tacctatgcc ccagaaggca
     5881 agagaggaag actatttgga ctttttgtat gattaaggtt cttattggac ttttccctag
     5941 gttttttttt tttgttattg ttgttgttgt ttcgttttct agctatagga actatctggg
     6001 gaggggccca gtgggtcctc ggccagagcc ctctctaagg acaggttggg gagggttggg
     6061 gagggctgcc tgtgctggac tgaggcttgt gccactgggc ctttctgatt ttgcctccaa
     6121 aggagagcgc tgtgatacct acatgtgtaa ggaagggcct tccgtattgg ggttctgcca
     6181 aggacccgta ttcagggacc catgctcttt tggggggact tttcctcttg tcttccctac
     6241 tttattagga cttgccctga ataccatttt ctaccccttg cccctccatt ctcctggccc
     6301 ttctgggggt cagctggtct ctatgaatat gctgggggtg cttccccata ggtctctccc
     6361 ttcatttgtc tctggtggga caaaatactg actcagtcct tagatgtagt ttcacccaag
     6421 agcatcttgg ccctgggaag aggtccctag gctgcagatg ctactgactg cttgctaggt
     6481 agcctctgga aagcattccc catccatcac tccccacttc tttctgctgt gctgcttccc
     6541 tcccaaactc catttctgtc acccttttta taagactttt cctcattctg tggggccata
     6601 aacctattta gtctggagcc aaagggatgc cctatctgaa ggaaaggggc atggggtggg
     6661 ggattccatc aaaactgttg ttttttgccc catgattttt ctttggtcag taggaggctg
     6721 gattggagtg gtgattattc ccctggagct aagctcagga gcccgaaggg agagactgag
     6781 actgactccc ttatctcttc atattcttta ttccctacca gatggatttt tttttttttt
     6841 tttggagacg gagtctcgcc ctgtcgccag gctggagtgt agtggcatga tctcgactca
     6901 ctgcaaaatc tgcctcccgg gttcaagcga ttctcctacc tcagcctccc gagtagctgg
     6961 gattacaggc atgtgccacc acgccaagct aatttttgta tttttagtag agacggggtt
     7021 tcaccatgtt ggccaggatg gtctcgatct cttgacctcg tgatctgcct gccttggcct
     7081 cccaaagtgc tgggattaca ggcgtgagcc accatgcccc gccccagatg gattttacat
     7141 ttgctctttt gtgtttcgct ccaaagggtt gtcttcctcg ccaaaaggag ggagggactt
     7201 tgaatttgat atgaatcttt aaaaccagaa ttggctggat atttcccatg attgggaaaa
     7261 gagtgaaatg aggacattct gtaaactgtc cctccctaat tccaaggatc agaaactccc
     7321 cgttttgctg actcattcca taactggaga aagaagctcc attgaccgaa gccacagggc
     7381 agcatggaag tttaaatttt ctctaaaatt aaaatgccaa ggataaagct ggctgcttcc
     7441 aggaggggga agaggagtgg ggagtgggcg gtgaaacttt tccagatgaa cggaccataa
     7501 atgtgttact ggctttgtgc ctgtagctca ttttattatg acctatatgc tcctgattta
     7561 aagagatctg tgtactgttt acttcccact tcccagaatc ccttgtatct cctttctcgg
     7621 gaattgtatt ttctaataaa tgacatttga gaaaaaaa
//
