LOCUS       NM_017861               3038 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens phosphatidylinositol glycan anchor biosynthesis class
            X (PIGX), transcript variant 2, mRNA.
ACCESSION   NM_017861 XM_005269349
VERSION     NM_017861.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ashida,H., Hong,Y., Murakami,Y., Shishioh,N., Sugimoto,N.,
            Kim,Y.U., Maeda,Y. and Kinoshita,T.
  TITLE     Mammalian PIG-X and yeast Pbn1p are the essential components of
            glycosylphosphatidylinositol-mannosyltransferase I
  JOURNAL   Mol Biol Cell 16 (3), 1439-1448 (2005)
   PUBMED   15635094
  REMARK    GeneRIF: mammalian PIG-X and yeast Pbn1p are the essential
            components of glycosylphosphatidylinositol-mannosyltransferase I
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Kinoshita,T. and Inoue,N.
  TITLE     Dissecting and manipulating the pathway for
            glycosylphos-phatidylinositol-anchor biosynthesis
  JOURNAL   Curr Opin Chem Biol 4 (6), 632-638 (2000)
   PUBMED   11102867
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG715890.1, BC022542.1,
            AC055725.22 and BU740645.1.
            
            On Jun 2, 2019 this sequence version replaced NM_017861.3.
            
            Summary: This gene encodes a type I transmembrane protein in the
            endoplasmic reticulum (ER). The protein is an essential component
            of glycosylphosphatidylinositol-mannosyltransferase I, which
            transfers the first of the four mannoses in the GPI-anchor
            precursors during GPI-anchor biosynthesis. Studies in rat indicate
            that the protein is translated from a non-AUG translation
            initiation site. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the central coding region, compared to variant 1, resulting
            in an isoform (2) that is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC022542.1, SRR7346977.110303.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match       :: ENST00000392391.9/ ENSP00000376192.4
            non-AUG initiation codon :: inferred from conservation
            RefSeq Select criteria   :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-238               BG715890.1         10-247
            239-383             BC022542.1         202-346
            384-617             BG715890.1         393-626
            618-746             BC022542.1         581-709
            747-759             AC055725.22        98116-98128
            760-1738            BC022542.1         723-1701
            1739-2661           AC055725.22        101774-102696
            2662-3038           BU740645.1         12-388              c
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q29"
     gene            1..3038
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /note="phosphatidylinositol glycan anchor biosynthesis
                     class X"
                     /db_xref="GeneID:54965"
                     /db_xref="HGNC:HGNC:26046"
                     /db_xref="MIM:610276"
     exon            1..268
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..933
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; non-AUG (CUG) translation initiation codon;
                     GPI-mannosyltransferase subunit;
                     phosphatidylinositol-glycan biosynthesis class X protein"
                     /codon_start=1
                     /product="phosphatidylinositol-glycan biosynthesis class X
                     protein isoform 2 precursor"
                     /protein_id="NP_060331.3"
                     /db_xref="CCDS:CCDS3320.2"
                     /db_xref="GeneID:54965"
                     /db_xref="HGNC:HGNC:26046"
                     /db_xref="MIM:610276"
                     /translation="MAARVAAVRAAAWLLLGAATGLTRGPAAAFTAARSDAGIRAMCS
                     EIILRQEVLKDGFHRDLLIKVKFGESIEDLHTCRLLIKQDIPAGLYVDPYELASLRER
                     NITEAVMVSENFDIEAPNYLSKESEVLIYARRDSQCIDCFQAFLPVHCRYHRPHSEDG
                     EASIVVNNPDLLMFCDQEFPILKCWAHSEVAAPCALENEDICQWNKMKYKSVYKNVIL
                     QVPVGLTVHTSLVCSVTLLITILCSTLILVAVFKYGHFSL"
     exon            269..332
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            333..474
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            475..688
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            689..789
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /inference="alignment:Splign:2.1.0"
     exon            790..3038
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1909..1914
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /note="hexamer: AATAAA"
     polyA_site      1932
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /note="major polyA site"
     regulatory      3016..3021
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
                     /note="hexamer: AATAAA"
     polyA_site      3038
                     /gene="PIGX"
                     /gene_synonym="PIG-X"
ORIGIN      
        1 agctgggaac cgcgggcgct aggcgcgcgc acccagcact cggtcccagc cgataaatct
       61 ggggcagcgc gcggtaggag ctgcgggcgg ccaggcccct tcctgcgtcc gcacctggcc
      121 ccgcgcgccc ctctcgggcg tccggcttcc ggcgtcctgg cggctcgggt ggcggcggtt
      181 cgggcggccg cctggctgct cctcggggcg gcgaccgggc tcacgcgcgg gcccgccgcg
      241 gccttcaccg ccgcgcgctc tgacgccggc ataagggcca tgtgttctga aattattttg
      301 aggcaagaag ttttgaaaga tggtttccac agagaccttt taatcaaagt gaagtttggg
      361 gaaagcattg aggacttgca cacctgccgt ctcttaatta aacaggacat tcctgcagga
      421 ctttatgtgg atccgtatga gttggcttca ttacgagaga gaaacataac agaggcagtg
      481 atggtttcag aaaattttga tatagaggcc cctaactatt tgtccaagga gtctgaagtt
      541 ctcatttatg ccagacgaga ttcacagtgc attgactgtt ttcaagcctt tttgcctgtg
      601 cactgccgct atcatcggcc gcacagtgaa gatggagaag cctcgattgt ggtcaataac
      661 ccagatttgt tgatgttttg tgaccaagag ttcccgattt tgaaatgctg ggctcactca
      721 gaagtggcag ccccttgtgc tttggagaat gaggatatct gccaatggaa caagatgaag
      781 tataaatcag tatataagaa tgtgattcta caagttccag tgggactgac tgtacatacc
      841 tctctagtat gttctgtgac tctgctcatt acaatcctgt gctctacatt gatccttgta
      901 gcagttttca aatatggcca tttttcccta taagttttat gtagttaaat gcttcctaga
      961 aacctaaata agatctatta atttctgacg agaggtgttc ttctagaatt aattactttt
     1021 atcttttgtc ttcatttgtg gccaaaatta tgtttactag aggaaatttg ggatcattct
     1081 cagctaattc caaaatgtag tgctctattg catggatcct tggtaatcct caagcatcag
     1141 atgccataag gggaaactta attctgctaa attaatgttt attttgtgag aagtgacttt
     1201 atcttcattt ggggtagaaa aattatttct ttatgtagta gagacaaatt attctcattt
     1261 tgcaagtact ttcaatttaa gctacaaatt gagaaaaccg ttataaataa gaataaaata
     1321 ggccaggcac agtggctcac acctgtaatc ccagcacttt gggaggccga ggtgggcgga
     1381 tcaccagagg tcaagagttt gagaccagct tggtgaaacc ctgtctctac taaaaataca
     1441 aaagttagct ggggctggtg gtgggcatct gtagtcccag ctaattggaa gggtgaggcg
     1501 ggaggatcgc ttgaacctgg gaggcggagg ttccagagag ccaagatcgc accactgcac
     1561 tacagcctgg gcgacagaac gagaccctgt ctccaaagga aaaacaaaaa agaagaataa
     1621 aataatttgg atgaaaatca tgtttattta aatagtaatg tcatgagact attaaagatg
     1681 tgccagagtt tcaatgaaaa tcattaaagt aggacagcta agaaattaat attaatatca
     1741 aaattattga taatcttaaa ttattgatta ttccttaacg cactccattc tccttttaca
     1801 ttttatcatg tttcttttga atatatgaat tggcaaagga cttgatgaaa ctgagtacta
     1861 agatttggta cagagtatgt caggaagaca actcagattg ccattttaaa taaagttgta
     1921 catgaacaat aattggaatc atcaggtaat ttttttaaac aaaggttctt catttactgt
     1981 tatgattgga aaaaaattag aaaataaagt aagtgccata ggctaattaa aaaataaaac
     2041 cttggccggg cgcggtggct tacgcctata atcccagcac tttgggaggc cgagacgggc
     2101 agatcacgag gtcaggagat tgagaccatc ctggctaaca cggtgaaacc ccatctctac
     2161 taaaaataca aaaaaatgag ccgggtatgg tggtgcatac ctgtagtccc agctactcgg
     2221 gaggctgagg caggagaatg gcatgaaccc ggaaggcaga gcttgcagtg agccgagatc
     2281 acgccactgc actccagcct gggcgacaga gtgagactct gtctcaaaaa aaaaaaaaaa
     2341 aaaaaaaaaa aaaaagtaaa taaaacctta gggcaagcat gttccaaaac agaagacatc
     2401 aaaccagggt gtgttggcat tatttatttt atgtagagta ttgagatgtg ataaaccttt
     2461 gtatatgcca tcactgctcc atttcctaaa gtattttggt gttttcatgg gactacatct
     2521 tgtataattt gacatgtatg acctgactag tcctaacagt tctttttaaa cgtgtatttg
     2581 agacttaaat attggaaaag gtaacccagt gccataaagc agaaacagtg acctttaaaa
     2641 tgactaaaat ttcaacccag tatagtttca tacctatttc atgctttctc gtaagaaaga
     2701 atttcatgtt ttagaaacat ttctttcact atgggattaa taatatgaca ctgaaatgga
     2761 attatatatg tcagctacaa ttcaggcatt gttttgggat atggttagat tgacaggcaa
     2821 cgttttggaa tatggttaga cggggaggga acacggtact gtttaacatc ttaagagtat
     2881 gtgggggtat gtgtatacag aatgtttttg tttttgctca tcacaaccag gtttcatcag
     2941 tatttaaaat atttaagatt gtggcttgga atgtggttca gccatttact cacttataca
     3001 aatattcaga agtgtaataa aaatgtgaat gaagaaaa
//
