LOCUS       NM_017837               4673 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens phosphatidylinositol glycan anchor biosynthesis class
            V (PIGV), transcript variant 2, mRNA.
ACCESSION   NM_017837
VERSION     NM_017837.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Horn,D., Wieczorek,D., Metcalfe,K., Baric,I., Palezac,L., Cuk,M.,
            Petkovic Ramadza,D., Kruger,U., Demuth,S., Heinritz,W., Linden,T.,
            Koenig,J., Robinson,P.N. and Krawitz,P.
  TITLE     Delineation of PIGV mutation spectrum and associated phenotypes in
            hyperphosphatasia with mental retardation syndrome
  JOURNAL   Eur J Hum Genet 22 (6), 762-767 (2014)
   PUBMED   24129430
  REMARK    GeneRIF: Data indicate that mannosyltransferases PIGV mutations are
            the major cause of hyperphosphatasia-mental retardation syndrome
            (HPMRS) which displays a broad clinical variability regarding
            associated malformations and growth patterns.
REFERENCE   3  (bases 1 to 4673)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   4  (bases 1 to 4673)
  AUTHORS   Hirata,T., Fujita,M., Kanzawa,N., Murakami,Y., Maeda,Y. and
            Kinoshita,T.
  TITLE     Glycosylphosphatidylinositol mannosyltransferase II is the
            rate-limiting enzyme in glycosylphosphatidylinositol biosynthesis
            under limited dolichol-phosphate mannose availability
  JOURNAL   J Biochem 154 (3), 257-264 (2013)
   PUBMED   23694781
  REMARK    GeneRIF: PIGV is the rate-limiting enzyme in GPI biosynthesis under
            limited dolicholphosphate mannose availability.
REFERENCE   5  (bases 1 to 4673)
  AUTHORS   Murakami,Y., Kanzawa,N., Saito,K., Krawitz,P.M., Mundlos,S.,
            Robinson,P.N., Karadimitris,A., Maeda,Y. and Kinoshita,T.
  TITLE     Mechanism for release of alkaline phosphatase caused by
            glycosylphosphatidylinositol deficiency in patients with
            hyperphosphatasia mental retardation syndrome
  JOURNAL   J Biol Chem 287 (9), 6318-6325 (2012)
   PUBMED   22228761
  REMARK    GeneRIF: Hyperphosphatasia resulted from secretion of ALP, a
            GPI-anchored protein normally expressed on the cell surface, into
            serum due to PIGV deficiency.
REFERENCE   6  (bases 1 to 4673)
  AUTHORS   Horn,D., Krawitz,P., Mannhardt,A., Korenke,G.C. and Meinecke,P.
  TITLE     Hyperphosphatasia-mental retardation syndrome due to PIGV
            mutations: expanded clinical spectrum
  JOURNAL   Am J Med Genet A 155A (8), 1917-1922 (2011)
   PUBMED   21739589
  REMARK    GeneRIF: novel compound heterozygous mutations in the PIGV gene
            c.467G>A and c.1022C>A and a homozygous mutation c.1022C>A in
            hyperphosphatasia-mental retardation syndrome
REFERENCE   7  (bases 1 to 4673)
  AUTHORS   Krawitz,P.M., Schweiger,M.R., Rodelsperger,C., Marcelis,C.,
            Kolsch,U., Meisel,C., Stephani,F., Kinoshita,T., Murakami,Y.,
            Bauer,S., Isau,M., Fischer,A., Dahl,A., Kerick,M., Hecht,J.,
            Kohler,S., Jager,M., Grunhagen,J., de Condor,B.J., Doelken,S.,
            Brunner,H.G., Meinecke,P., Passarge,E., Thompson,M.D., Cole,D.E.,
            Horn,D., Roscioli,T., Mundlos,S. and Robinson,P.N.
  TITLE     Identity-by-descent filtering of exome sequence data identifies
            PIGV mutations in hyperphosphatasia mental retardation syndrome
  JOURNAL   Nat Genet 42 (10), 827-829 (2010)
   PUBMED   20802478
  REMARK    GeneRIF: PIGV mutations are associated with hyperphosphatasia
            mental retardation syndrome.
REFERENCE   8  (bases 1 to 4673)
  AUTHORS   Kang,J.Y., Hong,Y., Ashida,H., Shishioh,N., Murakami,Y.,
            Morita,Y.S., Maeda,Y. and Kinoshita,T.
  TITLE     PIG-V involved in transferring the second mannose in
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 280 (10), 9489-9497 (2005)
   PUBMED   15623507
  REMARK    GeneRIF: PIG-V is the second mannosyltransferase in GPI anchor
            biosynthesis.
REFERENCE   9  (bases 1 to 4673)
  AUTHORS   Fabre,A.L., Orlean,P. and Taron,C.H.
  TITLE     Saccharomyces cerevisiae Ybr004c and its human homologue are
            required for addition of the second mannose during
            glycosylphosphatidylinositol precursor assembly
  JOURNAL   FEBS J 272 (5), 1160-1168 (2005)
   PUBMED   15720390
REFERENCE   10 (bases 1 to 4673)
  AUTHORS   Kinoshita,T. and Inoue,N.
  TITLE     Dissecting and manipulating the pathway for
            glycosylphos-phatidylinositol-anchor biosynthesis
  JOURNAL   Curr Opin Chem Biol 4 (6), 632-638 (2000)
   PUBMED   11102867
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL034380.26.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Oct 1, 2019 this sequence version replaced NM_017837.3.
            
            Summary: This gene encodes a mannosyltransferase enzyme involved in
            the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a
            complex glycolipid that functions as a membrane anchor for many
            proteins and plays a role in multiple cellular processes including
            protein sorting and signal transduction. The encoded protein is
            localized to the endoplasmic reticulum and transfers the second
            mannose to the GPI backbone. Mutations in this gene are associated
            with hyperphosphatasia cognitive disability syndrome. Alternatively
            spliced transcript variants have been observed for this gene.
            [provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1-6 all encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.717005.1, BC013568.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000674202.1/ ENSP00000501479.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-319               AL034380.26        107964-108282       c
            320-454             AL034380.26        105339-105473       c
            455-1576            AL034380.26        100998-102119       c
            1577-4673           AL034380.26        95573-98669         c
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.11"
     gene            1..4673
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="phosphatidylinositol glycan anchor biosynthesis
                     class V"
                     /db_xref="GeneID:55650"
                     /db_xref="HGNC:HGNC:26031"
                     /db_xref="MIM:610274"
     exon            1..319
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     exon            320..454
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..1858
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /EC_number="2.3.1.225"
                     /note="isoform a is encoded by transcript variant 2;
                     Ybr004c homolog; GPI mannosyltransferase 2; GPI
                     mannosyltransferase II; dol-P-Man dependent GPI
                     mannosyltransferase; dol-P-Man dependent GPI
                     mannosyltransferase II"
                     /codon_start=1
                     /product="GPI mannosyltransferase 2 isoform a"
                     /protein_id="NP_060307.2"
                     /db_xref="CCDS:CCDS287.1"
                     /db_xref="GeneID:55650"
                     /db_xref="HGNC:HGNC:26031"
                     /db_xref="MIM:610274"
                     /translation="MWPQDPSRKEVLRFAVSCRILTLMLQALFNAIIPDHHAEAFSPP
                     RLAPSGFVDQLVEGLLGGLSHWDAEHFLFIAEHGYLYEHNFAFFPGFPLALLVGTELL
                     RPLRGLLSLRSCLLISVASLNFLFFMLAAVALHDLGCLVLHCPHQSFYAALLFCLSPA
                     NVFLAAGYSEALFALLTFSAMGQLERGRVWTSVLLFAFATGVRSNGLVSVGFLMHSQC
                     QGFFSSLTMLNPLRQLFKLMASLFLSVFTLGLPFALFQYYAYTQFCLPGSARPIPEPL
                     VQLAVDKGYRIAEGNEPPWCFWDVPLIYSYIQDVYWNVGFLKYYELKQVPNFLLAAPV
                     AILVAWATWTYVTTHPWLCLTLGLQRSKNNKTLEKPDLGFLSPQVFVYVVHAAVLLLF
                     GGLCMHVQVLTRFLGSSTPIMYWFPAHLLQDQEPLLRSLKTVPWKPLAEDSPPGQKVP
                     RNPIMGLLYHWKTCSPVTRYILGYFLTYWLLGLLLHCNFLPWT"
     exon            455..1576
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..4673
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2286..2291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="hexamer: AATAAA"
     polyA_site      2311
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="major polyA site"
     regulatory      2390..2395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="hexamer: ATTAAA"
     polyA_site      2417
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
     regulatory      4653..4658
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="hexamer: AATAAA"
     polyA_site      4673
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
ORIGIN      
        1 gcaggcgcgg gccgcgtggg ccccggatgg aggagctgag gttcggtccc cgccggggag
       61 gtcgggacgg gcaggggctg aggaccccgc gccagcttgg gagccctgga gttctggcgg
      121 gagggaagct gtcaaatgga agccggtaga aatgagacag acgcccggag aagcggggag
      181 ccccgcccct ccgccatagc agcctcgggc gccgcctctt cctttccagc ctcccgccct
      241 cgtctgcttc cggccctgtg gcctggtggg gctctgcagg ctccctcggg agtggtcctt
      301 gggccgtggc ccctctggga ggcctgaggg agctcaatcc tggtagcaac acccctgaat
      361 tcctggtggt gaaaggatgt ggccccagga cccatcccgg aaggaggtgc tgaggtttgc
      421 agtcagctgc cgtatcctga ctctgatgct gcaggccctc ttcaatgcca tcatcccaga
      481 tcaccatgca gaagccttct ctcctcctcg cctggccccc tcaggctttg tggaccaact
      541 cgtggaaggt cttctgggcg gcctgtctca ctgggatgct gaacacttct tgttcattgc
      601 tgagcatggc tacctgtatg agcacaactt tgccttcttt cctggtttcc ccttggccct
      661 gctggtgggg actgaactgt tgagaccctt acgggggtta ctgagtctac gcagttgcct
      721 gctgatttcg gtagcatcac tcaatttctt gttcttcatg ttggctgcag ttgcacttca
      781 tgacctgggt tgtctggttt tgcactgtcc ccaccagtcc ttttatgcag ctctgctttt
      841 ctgtctcagc cctgccaatg tcttcctggc agctggttac tcagaagctt tgtttgccct
      901 cctgacattc agtgccatgg ggcagctgga gaggggccga gtctggacta gtgtactcct
      961 ctttgccttt gccactgggg tacgctccaa cgggctggtc agtgttggct tcctcatgca
     1021 ttctcaatgc caaggctttt tctcttctct aacgatgctg aatcctctga gacagctctt
     1081 taagctgatg gcctctctgt ttctgtcggt gttcacactt ggccttccct ttgccctctt
     1141 tcagtattat gcctacaccc aattctgtct gccaggctca gcccgcccca ttcctgagcc
     1201 tttggtacag ttagctgtag acaagggcta ccggattgca gagggaaatg aaccgccttg
     1261 gtgcttctgg gatgttccac taatatacag ctatatccag gatgtctact ggaatgttgg
     1321 ctttttgaaa tactatgagc tcaagcaggt gcccaatttt ctactggctg caccagtggc
     1381 tatactggtt gcctgggcaa cttggacata cgtgaccact cacccttggc tctgccttac
     1441 acttgggctg caaaggagca agaacaataa gaccctagag aagcccgatc ttggattcct
     1501 cagtcctcag gtgtttgtgt acgtggtcca cgctgcagtg ctgctgctgt ttggaggtct
     1561 gtgcatgcat gttcaggttc tcaccaggtt tttgggctcc tccactccta ttatgtactg
     1621 gtttccagct cacttgcttc aggatcaaga gccgctgttg agatccttaa agactgtgcc
     1681 ttggaagcct cttgcagagg actccccacc aggacaaaag gtccccagaa atcctatcat
     1741 gggacttttg tatcactgga aaacctgttc tccagtcaca cgatacattc taggctactt
     1801 cctgacttac tggctcctgg gactactcct acattgcaac ttcctgcctt ggacatgacc
     1861 tggactctcc agggacaggt tggaagccaa cttaacccag gggtctgaaa gtaaaaatac
     1921 acattggaac tgcctctgct gccctgggat cattactgtg tccattataa tctttctctt
     1981 tctctttgaa agctggtcag gaatgggaga agtgtcagac actagagagc cccttctggt
     2041 cctggctagg gcaaatttta gacaactatt ttctctgtaa gtgaagattg tcgtattcca
     2101 agtctaaaat acacctggat ctgtctagtc aatcaacata gcagagacag tcttaaacct
     2161 accattgacc tgtgtgtaaa tttaaatgtc aatttattga agtgtaaatt tcatcaaagg
     2221 cattagctga caggctggta acagtccaca caagatggta taggcctgaa cagtgtagtg
     2281 gcagtaataa agtgggacca ttttttccaa atgcgacatt gtttctgatg ttttttttta
     2341 tgatttgtgc atgtattttt atatgaaaaa aacttacaaa gatatgcaaa ttaaacttct
     2401 ttcagttata catgcaatga attgcctttt taaaaataat acaggccagg cacgacgttt
     2461 cacactgtaa tcccagcact ttgtgatgcc gaggtgggca gatcacttga ggccaggagt
     2521 ttgagaccgg cctggccaac atggcaaaac cccgtctcta ctaaaaatac aaaaattagc
     2581 cgtgcatgat ggtacacacc tgtaatccta ctcgggaggc tgaggcatga gaattgcttg
     2641 aacttggaag gcggaggttt cagtgagctg agtttgcgcc actgcgctcc agcctgggca
     2701 acagagtgag gctctgtctc aaaaaaagta aaaaataaaa ataatacaaa tctttccatg
     2761 tggcaaaaag taaaataatt ttaaaaagtt aaaataatat aaatcagctg ggggaaaaaa
     2821 gggtaagaag aaggcatcag gaaccctgga ttctagtcct ctcagtaaaa ccccatttac
     2881 cagccatgtg acttgggaga gttttcagaa ccttagttac cacctctgaa accatgggaa
     2941 atgatgatat tcttcagggg gtggtggtga atgatgcagt ctcttgtagg tccctttctg
     3001 ctctaacatc ttaagaactg ccttaaacaa cagaatcacc ccagtctaaa tgctaatgta
     3061 gaggcttaaa tgtgtgattt taggagaaaa tatccagttc tcctgttttc agcaattaga
     3121 agaaactcct cctccaccga gtgcaactct tgggagtaat ttaggtggtc tttcctttca
     3181 atgagggaaa gcagtaacat ctggcagata aggtgcctgt tgtgccgttc tcagatgcct
     3241 gcttacttga tcagtagaca tgggtcagag gcgccaacac ggcggcagga attggtaaat
     3301 tgtacgtaag tccaaggtgg aatgaaatgg tatctgccct gcttcctggc ccccgtttgg
     3361 tcatgagcct atcactataa tggtagtaag atcactggcc cctgctgagc tacttttgcc
     3421 cattccttga gccagacgag agtttggctc tgtatcatcc atcacctctt ttatttccca
     3481 tttaagccaa aacatccagt aagcagaaac tcttcaaaac agatgggtaa tcataagcag
     3541 cttctggaaa gcctcaagaa ttggaatttt gacacactcc ctctgaccat gacagaaagc
     3601 tcaacttgcc agctgaaggg aagctcctgg ctgagcccca gcaccctctg ttggagcttc
     3661 tggtcagcct ggccaataag catttcccat ccatcgcccc agcactagga cagctgagag
     3721 aatttggttc agagtcaaat gcttctgaaa atgtacacac agatcagggc agccctctgc
     3781 cttctgccaa gacaccaagt gcccttcttg gcaaacgatg atcacccctg ccctagtgtc
     3841 cctccagtta gttggcagaa actgttccct ttctccttgc caacagctgc aaatccagag
     3901 acttccacct tgcgtgcagg aagtgttggg gtcctgtttc agctctttat tgccgaggta
     3961 ggagcactgg gttcttcgct ctcctctttg cctccttttg ggggataatc aggactgatt
     4021 attcctctac tggaagaggt gactcagacc ccacagagta ggtgtgatgt cactgctctg
     4081 gcagtctcag tttaaccttc acactagatc tcatcatgcc cagaaacctt gtaacaggag
     4141 cccagctagg tgggtgcacc tggcatgagc tggacaaggt tttctctctg accttgcagg
     4201 gatttagttg ttctctaagg agctaaggaa tgggctgatt ttatgaccag cagctgtcct
     4261 tttaccatcc tattaacagt ctctttaata gtctgggaga gggacatgaa ggacccaagg
     4321 caggaagttt cctgtttacc ctgaggccta acaatgccac agctaggtgg ggtgtctaca
     4381 gtttcagaga aaagtcattt ccttctgaga ccctgctaaa acagaaacca gacgcctcag
     4441 cctcacgtac ctagaagtag atgctttttt ttgttcttta atgaaggtgg gaggagagtg
     4501 tttgacatac ctttaccagt cctcctggaa aaatgttact tttattgaat agaccctctc
     4561 actgtcatct gaaaatgtaa tatgtgcagg ctcaagggat ttgaaactga ttgattcttt
     4621 cagtaattgt ttattaatgc ctattttagg ggaataaaga tgactcagct atg
//
