LOCUS       NM_001374484            3985 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens phosphatidylinositol glycan anchor biosynthesis class
            V (PIGV), transcript variant 8, mRNA.
ACCESSION   NM_001374484
VERSION     NM_001374484.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Horn,D., Wieczorek,D., Metcalfe,K., Baric,I., Palezac,L., Cuk,M.,
            Petkovic Ramadza,D., Kruger,U., Demuth,S., Heinritz,W., Linden,T.,
            Koenig,J., Robinson,P.N. and Krawitz,P.
  TITLE     Delineation of PIGV mutation spectrum and associated phenotypes in
            hyperphosphatasia with mental retardation syndrome
  JOURNAL   Eur J Hum Genet 22 (6), 762-767 (2014)
   PUBMED   24129430
  REMARK    GeneRIF: Data indicate that mannosyltransferases PIGV mutations are
            the major cause of hyperphosphatasia-mental retardation syndrome
            (HPMRS) which displays a broad clinical variability regarding
            associated malformations and growth patterns.
REFERENCE   3  (bases 1 to 3985)
  AUTHORS   Willer,C.J., Schmidt,E.M., Sengupta,S., Peloso,G.M., Gustafsson,S.,
            Kanoni,S., Ganna,A., Chen,J., Buchkovich,M.L., Mora,S.,
            Beckmann,J.S., Bragg-Gresham,J.L., Chang,H.Y., Demirkan,A., Den
            Hertog,H.M., Do,R., Donnelly,L.A., Ehret,G.B., Esko,T.,
            Feitosa,M.F., Ferreira,T., Fischer,K., Fontanillas,P., Fraser,R.M.,
            Freitag,D.F., Gurdasani,D., Heikkila,K., Hypponen,E., Isaacs,A.,
            Jackson,A.U., Johansson,A., Johnson,T., Kaakinen,M., Kettunen,J.,
            Kleber,M.E., Li,X., Luan,J., Lyytikainen,L.P., Magnusson,P.K.E.,
            Mangino,M., Mihailov,E., Montasser,M.E., Muller-Nurasyid,M.,
            Nolte,I.M., O'Connell,J.R., Palmer,C.D., Perola,M., Petersen,A.K.,
            Sanna,S., Saxena,R., Service,S.K., Shah,S., Shungin,D., Sidore,C.,
            Song,C., Strawbridge,R.J., Surakka,I., Tanaka,T., Teslovich,T.M.,
            Thorleifsson,G., Van den Herik,E.G., Voight,B.F., Volcik,K.A.,
            Waite,L.L., Wong,A., Wu,Y., Zhang,W., Absher,D., Asiki,G.,
            Barroso,I., Been,L.F., Bolton,J.L., Bonnycastle,L.L., Brambilla,P.,
            Burnett,M.S., Cesana,G., Dimitriou,M., Doney,A.S.F., Doring,A.,
            Elliott,P., Epstein,S.E., Ingi Eyjolfsson,G., Gigante,B.,
            Goodarzi,M.O., Grallert,H., Gravito,M.L., Groves,C.J., Hallmans,G.,
            Hartikainen,A.L., Hayward,C., Hernandez,D., Hicks,A.A., Holm,H.,
            Hung,Y.J., Illig,T., Jones,M.R., Kaleebu,P., Kastelein,J.J.P.,
            Khaw,K.T., Kim,E., Klopp,N., Komulainen,P., Kumari,M.,
            Langenberg,C., Lehtimaki,T., Lin,S.Y., Lindstrom,J., Loos,R.J.F.,
            Mach,F., McArdle,W.L., Meisinger,C., Mitchell,B.D., Muller,G.,
            Nagaraja,R., Narisu,N., Nieminen,T.V.M., Nsubuga,R.N., Olafsson,I.,
            Ong,K.K., Palotie,A., Papamarkou,T., Pomilla,C., Pouta,A.,
            Rader,D.J., Reilly,M.P., Ridker,P.M., Rivadeneira,F., Rudan,I.,
            Ruokonen,A., Samani,N., Scharnagl,H., Seeley,J., Silander,K.,
            Stancakova,A., Stirrups,K., Swift,A.J., Tiret,L.,
            Uitterlinden,A.G., van Pelt,L.J., Vedantam,S., Wainwright,N.,
            Wijmenga,C., Wild,S.H., Willemsen,G., Wilsgaard,T., Wilson,J.F.,
            Young,E.H., Zhao,J.H., Adair,L.S., Arveiler,D., Assimes,T.L.,
            Bandinelli,S., Bennett,F., Bochud,M., Boehm,B.O., Boomsma,D.I.,
            Borecki,I.B., Bornstein,S.R., Bovet,P., Burnier,M., Campbell,H.,
            Chakravarti,A., Chambers,J.C., Chen,Y.I., Collins,F.S.,
            Cooper,R.S., Danesh,J., Dedoussis,G., de Faire,U., Feranil,A.B.,
            Ferrieres,J., Ferrucci,L., Freimer,N.B., Gieger,C., Groop,L.C.,
            Gudnason,V., Gyllensten,U., Hamsten,A., Harris,T.B., Hingorani,A.,
            Hirschhorn,J.N., Hofman,A., Hovingh,G.K., Hsiung,C.A.,
            Humphries,S.E., Hunt,S.C., Hveem,K., Iribarren,C., Jarvelin,M.R.,
            Jula,A., Kahonen,M., Kaprio,J., Kesaniemi,A., Kivimaki,M.,
            Kooner,J.S., Koudstaal,P.J., Krauss,R.M., Kuh,D., Kuusisto,J.,
            Kyvik,K.O., Laakso,M., Lakka,T.A., Lind,L., Lindgren,C.M.,
            Martin,N.G., Marz,W., McCarthy,M.I., McKenzie,C.A., Meneton,P.,
            Metspalu,A., Moilanen,L., Morris,A.D., Munroe,P.B., Njolstad,I.,
            Pedersen,N.L., Power,C., Pramstaller,P.P., Price,J.F., Psaty,B.M.,
            Quertermous,T., Rauramaa,R., Saleheen,D., Salomaa,V.,
            Sanghera,D.K., Saramies,J., Schwarz,P.E.H., Sheu,W.H.,
            Shuldiner,A.R., Siegbahn,A., Spector,T.D., Stefansson,K.,
            Strachan,D.P., Tayo,B.O., Tremoli,E., Tuomilehto,J., Uusitupa,M.,
            van Duijn,C.M., Vollenweider,P., Wallentin,L., Wareham,N.J.,
            Whitfield,J.B., Wolffenbuttel,B.H.R., Ordovas,J.M., Boerwinkle,E.,
            Palmer,C.N.A., Thorsteinsdottir,U., Chasman,D.I., Rotter,J.I.,
            Franks,P.W., Ripatti,S., Cupples,L.A., Sandhu,M.S., Rich,S.S.,
            Boehnke,M., Deloukas,P., Kathiresan,S., Mohlke,K.L., Ingelsson,E.
            and Abecasis,G.R.
  CONSRTM   Global Lipids Genetics Consortium
  TITLE     Discovery and refinement of loci associated with lipid levels
  JOURNAL   Nat Genet 45 (11), 1274-1283 (2013)
   PUBMED   24097068
REFERENCE   4  (bases 1 to 3985)
  AUTHORS   Hirata,T., Fujita,M., Kanzawa,N., Murakami,Y., Maeda,Y. and
            Kinoshita,T.
  TITLE     Glycosylphosphatidylinositol mannosyltransferase II is the
            rate-limiting enzyme in glycosylphosphatidylinositol biosynthesis
            under limited dolichol-phosphate mannose availability
  JOURNAL   J Biochem 154 (3), 257-264 (2013)
   PUBMED   23694781
  REMARK    GeneRIF: PIGV is the rate-limiting enzyme in GPI biosynthesis under
            limited dolicholphosphate mannose availability.
REFERENCE   5  (bases 1 to 3985)
  AUTHORS   Murakami,Y., Kanzawa,N., Saito,K., Krawitz,P.M., Mundlos,S.,
            Robinson,P.N., Karadimitris,A., Maeda,Y. and Kinoshita,T.
  TITLE     Mechanism for release of alkaline phosphatase caused by
            glycosylphosphatidylinositol deficiency in patients with
            hyperphosphatasia mental retardation syndrome
  JOURNAL   J Biol Chem 287 (9), 6318-6325 (2012)
   PUBMED   22228761
  REMARK    GeneRIF: Hyperphosphatasia resulted from secretion of ALP, a
            GPI-anchored protein normally expressed on the cell surface, into
            serum due to PIGV deficiency.
REFERENCE   6  (bases 1 to 3985)
  AUTHORS   Horn,D., Krawitz,P., Mannhardt,A., Korenke,G.C. and Meinecke,P.
  TITLE     Hyperphosphatasia-mental retardation syndrome due to PIGV
            mutations: expanded clinical spectrum
  JOURNAL   Am J Med Genet A 155A (8), 1917-1922 (2011)
   PUBMED   21739589
  REMARK    GeneRIF: novel compound heterozygous mutations in the PIGV gene
            c.467G>A and c.1022C>A and a homozygous mutation c.1022C>A in
            hyperphosphatasia-mental retardation syndrome
REFERENCE   7  (bases 1 to 3985)
  AUTHORS   Krawitz,P.M., Schweiger,M.R., Rodelsperger,C., Marcelis,C.,
            Kolsch,U., Meisel,C., Stephani,F., Kinoshita,T., Murakami,Y.,
            Bauer,S., Isau,M., Fischer,A., Dahl,A., Kerick,M., Hecht,J.,
            Kohler,S., Jager,M., Grunhagen,J., de Condor,B.J., Doelken,S.,
            Brunner,H.G., Meinecke,P., Passarge,E., Thompson,M.D., Cole,D.E.,
            Horn,D., Roscioli,T., Mundlos,S. and Robinson,P.N.
  TITLE     Identity-by-descent filtering of exome sequence data identifies
            PIGV mutations in hyperphosphatasia mental retardation syndrome
  JOURNAL   Nat Genet 42 (10), 827-829 (2010)
   PUBMED   20802478
  REMARK    GeneRIF: PIGV mutations are associated with hyperphosphatasia
            mental retardation syndrome.
REFERENCE   8  (bases 1 to 3985)
  AUTHORS   Kang,J.Y., Hong,Y., Ashida,H., Shishioh,N., Murakami,Y.,
            Morita,Y.S., Maeda,Y. and Kinoshita,T.
  TITLE     PIG-V involved in transferring the second mannose in
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 280 (10), 9489-9497 (2005)
   PUBMED   15623507
  REMARK    GeneRIF: PIG-V is the second mannosyltransferase in GPI anchor
            biosynthesis.
REFERENCE   9  (bases 1 to 3985)
  AUTHORS   Fabre,A.L., Orlean,P. and Taron,C.H.
  TITLE     Saccharomyces cerevisiae Ybr004c and its human homologue are
            required for addition of the second mannose during
            glycosylphosphatidylinositol precursor assembly
  JOURNAL   FEBS J 272 (5), 1160-1168 (2005)
   PUBMED   15720390
REFERENCE   10 (bases 1 to 3985)
  AUTHORS   Kinoshita,T. and Inoue,N.
  TITLE     Dissecting and manipulating the pathway for
            glycosylphos-phatidylinositol-anchor biosynthesis
  JOURNAL   Curr Opin Chem Biol 4 (6), 632-638 (2000)
   PUBMED   11102867
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL034380.26.
            
            Summary: This gene encodes a mannosyltransferase enzyme involved in
            the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is a
            complex glycolipid that functions as a membrane anchor for many
            proteins and plays a role in multiple cellular processes including
            protein sorting and signal transduction. The encoded protein is
            localized to the endoplasmic reticulum and transfers the second
            mannose to the GPI backbone. Mutations in this gene are associated
            with hyperphosphatasia cognitive disability syndrome. Alternatively
            spliced transcript variants have been observed for this gene.
            [provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (8), as well as variant 9, encodes
            isoform c.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.118227.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-258               AL034380.26        108426-108683       c
            259-393             AL034380.26        105339-105473       c
            394-487             AL034380.26        102026-102119       c
            488-888             AL034380.26        100998-101398       c
            889-3985            AL034380.26        95573-98669         c
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.11"
     gene            1..3985
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="phosphatidylinositol glycan anchor biosynthesis
                     class V"
                     /db_xref="GeneID:55650"
                     /db_xref="HGNC:HGNC:26031"
                     /db_xref="MIM:610274"
     exon            1..258
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     exon            259..393
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     CDS             316..1170
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /EC_number="2.3.1.225"
                     /note="isoform c is encoded by transcript variant 8;
                     Ybr004c homolog; GPI mannosyltransferase 2; GPI
                     mannosyltransferase II; dol-P-Man dependent GPI
                     mannosyltransferase; dol-P-Man dependent GPI
                     mannosyltransferase II"
                     /codon_start=1
                     /product="GPI mannosyltransferase 2 isoform c"
                     /protein_id="NP_001361413.1"
                     /db_xref="CCDS:CCDS90892.1"
                     /db_xref="GeneID:55650"
                     /db_xref="HGNC:HGNC:26031"
                     /db_xref="MIM:610274"
                     /translation="MWPQDPSRKEVLRFAVSCRILTLMLQALFNAIIPDHHAEAFSPP
                     RLAPSGFVDQLVEGSARPIPEPLVQLAVDKGYRIAEGNEPPWCFWDVPLIYSYIQDVY
                     WNVGFLKYYELKQVPNFLLAAPVAILVAWATWTYVTTHPWLCLTLGLQRSKNNKTLEK
                     PDLGFLSPQVFVYVVHAAVLLLFGGLCMHVQVLTRFLGSSTPIMYWFPAHLLQDQEPL
                     LRSLKTVPWKPLAEDSPPGQKVPRNPIMGLLYHWKTCSPVTRYILGYFLTYWLLGLLL
                     HCNFLPWT"
     exon            394..487
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     exon            488..888
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     exon            889..3985
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1598..1603
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="hexamer: AATAAA"
     polyA_site      1623
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="major polyA site"
     regulatory      1702..1707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="hexamer: ATTAAA"
     polyA_site      1729
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
     regulatory      3965..3970
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
                     /note="hexamer: AATAAA"
     polyA_site      3985
                     /gene="PIGV"
                     /gene_synonym="GPI-MT-II; HPMRS1; PIG-V"
ORIGIN      
        1 attgtgtgtc aggccctgtg ctgagctgtt gttgcctgac agcaggaaga gcgcggctct
       61 ggtttctgtg aagaggctgc gggtcagcct ccgactacaa gagcctcggc ccacaccggg
      121 aactcagtga acgtcagcgg gagctggggc aagagcactg actctgagac agcagcctcc
      181 gctcactatt tgaaggcggg gtggcggggg gccttgaagg agagcgaagt gcgagagaac
      241 tcttcaggtg tcaccaagag gcctgaggga gctcaatcct ggtagcaaca cccctgaatt
      301 cctggtggtg aaaggatgtg gccccaggac ccatcccgga aggaggtgct gaggtttgca
      361 gtcagctgcc gtatcctgac tctgatgctg caggccctct tcaatgccat catcccagat
      421 caccatgcag aagccttctc tcctcctcgc ctggccccct caggctttgt ggaccaactc
      481 gtggaaggct cagcccgccc cattcctgag cctttggtac agttagctgt agacaagggc
      541 taccggattg cagagggaaa tgaaccgcct tggtgcttct gggatgttcc actaatatac
      601 agctatatcc aggatgtcta ctggaatgtt ggctttttga aatactatga gctcaagcag
      661 gtgcccaatt ttctactggc tgcaccagtg gctatactgg ttgcctgggc aacttggaca
      721 tacgtgacca ctcacccttg gctctgcctt acacttgggc tgcaaaggag caagaacaat
      781 aagaccctag agaagcccga tcttggattc ctcagtcctc aggtgtttgt gtacgtggtc
      841 cacgctgcag tgctgctgct gtttggaggt ctgtgcatgc atgttcaggt tctcaccagg
      901 tttttgggct cctccactcc tattatgtac tggtttccag ctcacttgct tcaggatcaa
      961 gagccgctgt tgagatcctt aaagactgtg ccttggaagc ctcttgcaga ggactcccca
     1021 ccaggacaaa aggtccccag aaatcctatc atgggacttt tgtatcactg gaaaacctgt
     1081 tctccagtca cacgatacat tctaggctac ttcctgactt actggctcct gggactactc
     1141 ctacattgca acttcctgcc ttggacatga cctggactct ccagggacag gttggaagcc
     1201 aacttaaccc aggggtctga aagtaaaaat acacattgga actgcctctg ctgccctggg
     1261 atcattactg tgtccattat aatctttctc tttctctttg aaagctggtc aggaatggga
     1321 gaagtgtcag acactagaga gccccttctg gtcctggcta gggcaaattt tagacaacta
     1381 ttttctctgt aagtgaagat tgtcgtattc caagtctaaa atacacctgg atctgtctag
     1441 tcaatcaaca tagcagagac agtcttaaac ctaccattga cctgtgtgta aatttaaatg
     1501 tcaatttatt gaagtgtaaa tttcatcaaa ggcattagct gacaggctgg taacagtcca
     1561 cacaagatgg tataggcctg aacagtgtag tggcagtaat aaagtgggac cattttttcc
     1621 aaatgcgaca ttgtttctga tgtttttttt tatgatttgt gcatgtattt ttatatgaaa
     1681 aaaacttaca aagatatgca aattaaactt ctttcagtta tacatgcaat gaattgcctt
     1741 tttaaaaata atacaggcca ggcacgacgt ttcacactgt aatcccagca ctttgtgatg
     1801 ccgaggtggg cagatcactt gaggccagga gtttgagacc ggcctggcca acatggcaaa
     1861 accccgtctc tactaaaaat acaaaaatta gccgtgcatg atggtacaca cctgtaatcc
     1921 tactcgggag gctgaggcat gagaattgct tgaacttgga aggcggaggt ttcagtgagc
     1981 tgagtttgcg ccactgcgct ccagcctggg caacagagtg aggctctgtc tcaaaaaaag
     2041 taaaaaataa aaataataca aatctttcca tgtggcaaaa agtaaaataa ttttaaaaag
     2101 ttaaaataat ataaatcagc tgggggaaaa aagggtaaga agaaggcatc aggaaccctg
     2161 gattctagtc ctctcagtaa aaccccattt accagccatg tgacttggga gagttttcag
     2221 aaccttagtt accacctctg aaaccatggg aaatgatgat attcttcagg gggtggtggt
     2281 gaatgatgca gtctcttgta ggtccctttc tgctctaaca tcttaagaac tgccttaaac
     2341 aacagaatca ccccagtcta aatgctaatg tagaggctta aatgtgtgat tttaggagaa
     2401 aatatccagt tctcctgttt tcagcaatta gaagaaactc ctcctccacc gagtgcaact
     2461 cttgggagta atttaggtgg tctttccttt caatgaggga aagcagtaac atctggcaga
     2521 taaggtgcct gttgtgccgt tctcagatgc ctgcttactt gatcagtaga catgggtcag
     2581 aggcgccaac acggcggcag gaattggtaa attgtacgta agtccaaggt ggaatgaaat
     2641 ggtatctgcc ctgcttcctg gcccccgttt ggtcatgagc ctatcactat aatggtagta
     2701 agatcactgg cccctgctga gctacttttg cccattcctt gagccagacg agagtttggc
     2761 tctgtatcat ccatcacctc ttttatttcc catttaagcc aaaacatcca gtaagcagaa
     2821 actcttcaaa acagatgggt aatcataagc agcttctgga aagcctcaag aattggaatt
     2881 ttgacacact ccctctgacc atgacagaaa gctcaacttg ccagctgaag ggaagctcct
     2941 ggctgagccc cagcaccctc tgttggagct tctggtcagc ctggccaata agcatttccc
     3001 atccatcgcc ccagcactag gacagctgag agaatttggt tcagagtcaa atgcttctga
     3061 aaatgtacac acagatcagg gcagccctct gccttctgcc aagacaccaa gtgcccttct
     3121 tggcaaacga tgatcacccc tgccctagtg tccctccagt tagttggcag aaactgttcc
     3181 ctttctcctt gccaacagct gcaaatccag agacttccac cttgcgtgca ggaagtgttg
     3241 gggtcctgtt tcagctcttt attgccgagg taggagcact gggttcttcg ctctcctctt
     3301 tgcctccttt tgggggataa tcaggactga ttattcctct actggaagag gtgactcaga
     3361 ccccacagag taggtgtgat gtcactgctc tggcagtctc agtttaacct tcacactaga
     3421 tctcatcatg cccagaaacc ttgtaacagg agcccagcta ggtgggtgca cctggcatga
     3481 gctggacaag gttttctctc tgaccttgca gggatttagt tgttctctaa ggagctaagg
     3541 aatgggctga ttttatgacc agcagctgtc cttttaccat cctattaaca gtctctttaa
     3601 tagtctggga gagggacatg aaggacccaa ggcaggaagt ttcctgttta ccctgaggcc
     3661 taacaatgcc acagctaggt ggggtgtcta cagtttcaga gaaaagtcat ttccttctga
     3721 gaccctgcta aaacagaaac cagacgcctc agcctcacgt acctagaagt agatgctttt
     3781 ttttgttctt taatgaaggt gggaggagag tgtttgacat acctttacca gtcctcctgg
     3841 aaaaatgtta cttttattga atagaccctc tcactgtcat ctgaaaatgt aatatgtgca
     3901 ggctcaaggg atttgaaact gattgattct ttcagtaatt gtttattaat gcctatttta
     3961 ggggaataaa gatgactcag ctatg
//
