LOCUS       NP_536724                435 aa            linear   PRI 27-MAR-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class U protein
            [Homo sapiens].
ACCESSION   NP_536724
VERSION     NP_536724.1
DBSOURCE    REFSEQ: accession NM_080476.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 435)
  AUTHORS   Xu,Y., Li,T., Zhou,Z., Hong,J., Chao,Y., Zhu,Z., Zhang,Y., Qu,Q.
            and Li,D.
  TITLE     Structures of liganded glycosylphosphatidylinositol transamidase
            illuminate GPI-AP biogenesis
  JOURNAL   Nat Commun 14 (1), 5520 (2023)
   PUBMED   37684232
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 435)
  AUTHORS   Xu,Y., Jia,G., Li,T., Zhou,Z., Luo,Y., Chao,Y., Bao,J., Su,Z.,
            Qu,Q. and Li,D.
  TITLE     Molecular insights into biogenesis of glycosylphosphatidylinositol
            anchor proteins
  JOURNAL   Nat Commun 13 (1), 2617 (2022)
   PUBMED   35551457
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 435)
  AUTHORS   Zhang,H., Su,J., Li,B., Gao,Y., Liu,M., He,L., Xu,H., Dong,Y.,
            Zhang,X.C. and Zhao,Y.
  TITLE     Structure of human glycosylphosphatidylinositol transamidase
  JOURNAL   Nat Struct Mol Biol 29 (3), 203-209 (2022)
   PUBMED   35165458
REFERENCE   4  (residues 1 to 435)
  AUTHORS   Liu,S.S., Jin,F., Liu,Y.S., Murakami,Y., Sugita,Y., Kato,T.,
            Gao,X.D., Kinoshita,T., Hattori,M. and Fujita,M.
  TITLE     Functional Analysis of the GPI Transamidase Complex by Screening
            for Amino Acid Mutations in Each Subunit
  JOURNAL   Molecules 26 (18), 5462 (2021)
   PUBMED   34576938
  REMARK    GeneRIF: Functional Analysis of the GPI Transamidase Complex by
            Screening for Amino Acid Mutations in Each Subunit.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 435)
  AUTHORS   Wei,X., Yang,W., Zhang,F., Cheng,F., Rao,J. and Lu,L.
  TITLE     PIGU promotes hepatocellular carcinoma progression through
            activating NF-kappaB pathway and increasing immune escape
  JOURNAL   Life Sci 260, 118476 (2020)
   PUBMED   32971102
  REMARK    GeneRIF: PIGU promotes hepatocellular carcinoma progression through
            activating NF-kappaB pathway and increasing immune escape.
REFERENCE   6  (residues 1 to 435)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   7  (residues 1 to 435)
  AUTHORS   Hong,Y., Ohishi,K., Kang,J.Y., Tanaka,S., Inoue,N., Nishimura,J.,
            Maeda,Y. and Kinoshita,T.
  TITLE     Human PIG-U and yeast Cdc91p are the fifth subunit of GPI
            transamidase that attaches GPI-anchors to proteins
  JOURNAL   Mol Biol Cell 14 (5), 1780-1789 (2003)
   PUBMED   12802054
REFERENCE   8  (residues 1 to 435)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   9  (residues 1 to 435)
  AUTHORS   Ohishi,K., Inoue,N. and Kinoshita,T.
  TITLE     PIG-S and PIG-T, essential for GPI anchor attachment to proteins,
            form a complex with GAA1 and GPI8
  JOURNAL   EMBO J 20 (15), 4088-4098 (2001)
   PUBMED   11483512
REFERENCE   10 (residues 1 to 435)
  AUTHORS   Ploug,M., Ronne,E., Behrendt,N., Jensen,A.L., Blasi,F. and Dano,K.
  TITLE     Cellular receptor for urokinase plasminogen activator.
            Carboxyl-terminal processing and membrane anchoring by
            glycosyl-phosphatidylinositol
  JOURNAL   J Biol Chem 266 (3), 1926-1933 (1991)
   PUBMED   1846368
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY422169.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: The protein encoded by this gene shares similarity with
            Saccharomyces cerevisiae Cdc91, a predicted integral membrane
            protein that may function in cell division control. The protein
            encoded by this gene is the fifth subunit of GPI transamidase that
            attaches GPI-anchors to proteins. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY422169.1, BC030512.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000217446.8/ ENSP00000217446.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.22"
     Protein         1..435
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class U protein"
                     /note="CDC91 (cell division cycle 91, S. cerevisiae,
                     homolog)-like 1; GPI transamidase subunit; cell division
                     cycle 91-like 1 protein; protein CDC91-like 1; GPI
                     transamidase component PIG-U; cell division cycle protein
                     91-like 1; GPI transamidase subunit PIGU; GPI and Actin
                     Bar homolog"
                     /calculated_mol_wt=49921
     Region          20..394
                     /region_name="PIG-U"
                     /note="GPI transamidase subunit PIG-U; pfam06728"
                     /db_xref="CDD:429085"
     Site            66..86
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            166..186
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            188..208
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            237..257
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            259..279
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Region          269..289
                     /region_name="May be involved in recognition of long-chain
                     fatty acids in GPI"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            286..306
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            313..333
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            355..375
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     Site            386..406
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9H490.3)"
     CDS             1..435
                     /gene="PIGU"
                     /gene_synonym="CDC91L1; GAB1; GPIBD21; NEDBSS; PIG-U"
                     /coded_by="NM_080476.5:8..1315"
                     /db_xref="CCDS:CCDS13239.1"
                     /db_xref="GeneID:128869"
                     /db_xref="HGNC:HGNC:15791"
                     /db_xref="MIM:608528"
ORIGIN      
        1 maaplvlvlv vavtvraalf rsslaefise rvevvsplss wkrvveglsl ldlgvspysg
       61 avfhetplii ylfhflidya elvfmitdal taialyfaiq dfnkvvfkkq kllleldqya
      121 pdvaelirtp memryiplkv alfyllnpyt ilscvakstc ainntliaff ilttikgsaf
      181 lsaiflalat yqslypltlf vpgllyllqr qyipvkmksk afwifsweya mmyvgslvvi
      241 iclsffllss wdfipavygf ilsvpdltpn iglfwyffae mfehfslffv cvfqinvffy
      301 tiplaiklke hpiffmfiqi aviaifksyp tvgdvalyma ffpvwnhlyr flrnifvltc
      361 iiivcsllfp vlwhlwiyag sansnffyai tltfnvgqil lisdyfyafl rreyylthgl
      421 yltakdgtea mlvlk
//
