LOCUS       NM_032634               4112 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens phosphatidylinositol glycan anchor biosynthesis class
            O (PIGO), transcript variant 1, mRNA.
ACCESSION   NM_032634
VERSION     NM_032634.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Tanigawa,J., Mimatsu,H., Mizuno,S., Okamoto,N., Fukushi,D.,
            Tominaga,K., Kidokoro,H., Muramatsu,Y., Nishi,E., Nakamura,S.,
            Motooka,D., Nomura,N., Hayasaka,K., Niihori,T., Aoki,Y.,
            Nabatame,S., Hayakawa,M., Natsume,J., Ozono,K., Kinoshita,T.,
            Wakamatsu,N. and Murakami,Y.
  TITLE     Phenotype-genotype correlations of PIGO deficiency with variable
            phenotypes from infantile lethality to mild learning difficulties
  JOURNAL   Hum Mutat 38 (7), 805-815 (2017)
   PUBMED   28337824
  REMARK    GeneRIF: PIGO deficiency shows variable phenotypes from infantile
            lethality to mild learning difficulties
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Pagnamenta,A.T., Murakami,Y., Taylor,J.M., Anzilotti,C.,
            Howard,M.F., Miller,V., Johnson,D.S., Tadros,S., Mansour,S.,
            Temple,I.K., Firth,R., Rosser,E., Harrison,R.E., Kerr,B.,
            Popitsch,N., Kinoshita,T., Taylor,J.C. and Kini,U.
  CONSRTM   DDD Study
  TITLE     Analysis of exome data for 4293 trios suggests GPI-anchor
            biogenesis defects are a rare cause of developmental disorders
  JOURNAL   Eur J Hum Genet 25 (6), 669-679 (2017)
   PUBMED   28327575
  REMARK    GeneRIF: Disease associated PIGO missense mutations resulted in
            decreased activity in vitro.
REFERENCE   3  (bases 1 to 4112)
  AUTHORS   Nakamura,K., Osaka,H., Murakami,Y., Anzai,R., Nishiyama,K.,
            Kodera,H., Nakashima,M., Tsurusaki,Y., Miyake,N., Kinoshita,T.,
            Matsumoto,N. and Saitsu,H.
  TITLE     PIGO mutations in intractable epilepsy and severe developmental
            delay with mild elevation of alkaline phosphatase levels
  JOURNAL   Epilepsia 55 (2), e13-e17 (2014)
   PUBMED   24417746
  REMARK    GeneRIF: Novel PIGO mutations expand the clinical spectrum of PIGO
            abnormalities to include epileptic encephalopathy with mild
            elevation of alkaline phosphatase (ALP).
REFERENCE   4  (bases 1 to 4112)
  AUTHORS   Kuki,I., Takahashi,Y., Okazaki,S., Kawawaki,H., Ehara,E., Inoue,N.,
            Kinoshita,T. and Murakami,Y.
  TITLE     Vitamin B6-responsive epilepsy due to inherited GPI deficiency
  JOURNAL   Neurology 81 (16), 1467-1469 (2013)
   PUBMED   24049131
  REMARK    Erratum:[Neurology. 2013 Nov 12;81(20):1803]
REFERENCE   5  (bases 1 to 4112)
  AUTHORS   Krawitz,P.M., Murakami,Y., Hecht,J., Kruger,U., Holder,S.E.,
            Mortier,G.R., Delle Chiaie,B., De Baere,E., Thompson,M.D.,
            Roscioli,T., Kielbasa,S., Kinoshita,T., Mundlos,S., Robinson,P.N.
            and Horn,D.
  TITLE     Mutations in PIGO, a member of the GPI-anchor-synthesis pathway,
            cause hyperphosphatasia with mental retardation
  JOURNAL   Am J Hum Genet 91 (1), 146-151 (2012)
   PUBMED   22683086
  REMARK    GeneRIF: Our data identify PIGO as the second gene associated with
            HPMRS and suggest that a deficiency in GPI-anchor synthesis is the
            underlying molecular pathomechanism of HPMRS.
REFERENCE   6  (bases 1 to 4112)
  AUTHORS   Humphray,S.J., Oliver,K., Hunt,A.R., Plumb,R.W., Loveland,J.E.,
            Howe,K.L., Andrews,T.D., Searle,S., Hunt,S.E., Scott,C.E.,
            Jones,M.C., Ainscough,R., Almeida,J.P., Ambrose,K.D., Ashwell,R.I.,
            Babbage,A.K., Babbage,S., Bagguley,C.L., Bailey,J., Banerjee,R.,
            Barker,D.J., Barlow,K.F., Bates,K., Beasley,H., Beasley,O.,
            Bird,C.P., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.,
            Burrill,W., Burton,J., Carder,C., Carter,N.P., Chapman,J.C.,
            Chen,Y., Clarke,G., Clark,S.Y., Clee,C.M., Clegg,S., Collier,R.E.,
            Corby,N., Crosier,M., Cummings,A.T., Davies,J., Dhami,P., Dunn,M.,
            Dutta,I., Dyer,L.W., Earthrowl,M.E., Faulkner,L., Fleming,C.J.,
            Frankish,A., Frankland,J.A., French,L., Fricker,D.G., Garner,P.,
            Garnett,J., Ghori,J., Gilbert,J.G., Glison,C., Grafham,D.V.,
            Gribble,S., Griffiths,C., Griffiths-Jones,S., Grocock,R., Guy,J.,
            Hall,R.E., Hammond,S., Harley,J.L., Harrison,E.S., Hart,E.A.,
            Heath,P.D., Henderson,C.D., Hopkins,B.L., Howard,P.J., Howden,P.J.,
            Huckle,E., Johnson,C., Johnson,D., Joy,A.A., Kay,M., Keenan,S.,
            Kershaw,J.K., Kimberley,A.M., King,A., Knights,A., Laird,G.K.,
            Langford,C., Lawlor,S., Leongamornlert,D.A., Leversha,M., Lloyd,C.,
            Lloyd,D.M., Lovell,J., Martin,S., Mashreghi-Mohammadi,M.,
            Matthews,L., McLaren,S., McLay,K.E., McMurray,A., Milne,S.,
            Nickerson,T., Nisbett,J., Nordsiek,G., Pearce,A.V., Peck,A.I.,
            Porter,K.M., Pandian,R., Pelan,S., Phillimore,B., Povey,S.,
            Ramsey,Y., Rand,V., Scharfe,M., Sehra,H.K., Shownkeen,R.,
            Sims,S.K., Skuce,C.D., Smith,M., Steward,C.A., Swarbreck,D.,
            Sycamore,N., Tester,J., Thorpe,A., Tracey,A., Tromans,A.,
            Thomas,D.W., Wall,M., Wallis,J.M., West,A.P., Whitehead,S.L.,
            Willey,D.L., Williams,S.A., Wilming,L., Wray,P.W., Young,L.,
            Ashurst,J.L., Coulson,A., Blocker,H., Durbin,R., Sulston,J.E.,
            Hubbard,T., Jackson,M.J., Bentley,D.R., Beck,S., Rogers,J. and
            Dunham,I.
  TITLE     DNA sequence and analysis of human chromosome 9
  JOURNAL   Nature 429 (6990), 369-374 (2004)
   PUBMED   15164053
REFERENCE   7  (bases 1 to 4112)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   8  (bases 1 to 4112)
  AUTHORS   Takeda,S., Kadowaki,S., Haga,T., Takaesu,H. and Mitaku,S.
  TITLE     Identification of G protein-coupled receptor genes from the human
            genome sequence
  JOURNAL   FEBS Lett 520 (1-3), 97-101 (2002)
   PUBMED   12044878
  REMARK    Erratum:[FEBS Lett 2002 Jul 17;523(1-3):257]
REFERENCE   9  (bases 1 to 4112)
  AUTHORS   Kinoshita,T. and Inoue,N.
  TITLE     Dissecting and manipulating the pathway for
            glycosylphos-phatidylinositol-anchor biosynthesis
  JOURNAL   Curr Opin Chem Biol 4 (6), 632-638 (2000)
   PUBMED   11102867
  REMARK    Review article
REFERENCE   10 (bases 1 to 4112)
  AUTHORS   Hong,Y., Maeda,Y., Watanabe,R., Inoue,N., Ohishi,K. and
            Kinoshita,T.
  TITLE     Requirement of PIG-F and PIG-O for transferring phosphoethanolamine
            to the third mannose in glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 275 (27), 20911-20919 (2000)
   PUBMED   10781593
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL353795.13, CR985224.1,
            AY358472.1, BC029271.1, BE139632.1 and BU727886.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_032634.3.
            
            Summary: This gene encodes a protein that is involved in
            glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The
            GPI-anchor is a glycolipid which contains three mannose molecules
            in its core backbone. The GPI-anchor is found on many blood cells
            and serves to anchor proteins to the cell surface. This protein is
            involved in the transfer of ethanolaminephosphate (EtNP) to the
            third mannose in GPI. At least three alternatively spliced
            transcripts encoding two distinct isoforms have been found for this
            gene. [provided by RefSeq, Jan 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.144472.1, AL833956.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378617.4/ ENSP00000367880.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                AL353795.13        66267-66308         c
            43-625              CR985224.1         28-610
            626-2441            AY358472.1         190-2005
            2442-3748           BC029271.1         1671-2977
            3749-4107           BE139632.1         1-359               c
            4108-4112           BU727886.1         18-22               c
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..4112
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /note="phosphatidylinositol glycan anchor biosynthesis
                     class O"
                     /db_xref="GeneID:84720"
                     /db_xref="HGNC:HGNC:23215"
                     /db_xref="MIM:614730"
     exon            1..437
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            438..949
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             439..3708
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /note="isoform 1 is encoded by transcript variant 1; GPI
                     ethanolamine phosphate transferase 3;
                     phosphatidylinositol-glycan biosynthesis class O protein"
                     /codon_start=1
                     /product="GPI ethanolamine phosphate transferase 3 isoform
                     1"
                     /protein_id="NP_116023.2"
                     /db_xref="CCDS:CCDS6575.1"
                     /db_xref="GeneID:84720"
                     /db_xref="HGNC:HGNC:23215"
                     /db_xref="MIM:614730"
                     /translation="MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEP
                     PGPGSLPWGSQGKPGACWMASRFSRVVLVLIDALRFDFAQPQHSHVPREPPVSLPFLG
                     KLSSLQRILEIQPHHARLYRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVE
                     DNLIKQLTSAGRRVVFMGDDTWKDLFPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPT
                     MDSGEWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAG
                     DHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLP
                     IPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKE
                     LHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFSLVR
                     MAGGTALLAASCFICLLASQWAISPGFPFCPLLLTPVAWGLVGAIAYAGLLGTIELKL
                     DLVLLGAVAAVSSFLPFLWKAWAGWGSKRPLATLFPIPGPVLLLLLFRLAVFFSDSFV
                     VAEARATPFLLGSFILLLVVQLHWEGQLLPPKLLTMPRLGTSATTNPPRHNGAYALRL
                     GIGLLLCTRLAGLFHRCPEETPVCHSSPWLSPLASMVGGRAKNLWYGACVAALVALLA
                     AVRLWLRRYGNLKSPEPPMLFVRWGLPLMALGTAAYWALASGADEAPPRLRVLVSGAS
                     MVLPRAVAGLAASGLALLLWKPVTVLVKAGAGAPRTRTVLTPFSGPPTSQADLDYVVP
                     QIYRHMQEEFRGRLERTKSQGPLTVAAYQLGSVYSAAMVTALTLLAFPLLLLHAERIS
                     LVFLLLFLQSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPA
                     IHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKR
                     QQPPGNEADARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALA
                     ASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQ
                     R"
     exon            950..1093
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1217
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1377
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1557
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..3085
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3086..3292
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3507
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3508..3578
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3579..4112
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4092..4097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /note="hexamer: AATAAA"
     polyA_site      4112
                     /gene="PIGO"
                     /gene_synonym="HPMRS2"
                     /note="major polyA site"
ORIGIN      
        1 gccgccgccg cacttccggg tgccattgca ggtgagccag ggtctcccga gaacctcacc
       61 ccccggtatt cccctcaccc tgaagtaagg gagtctccct cttcccctca gctcctaacc
      121 aaggtgctca gtaccgcggc tagggtgccc agcgggaggc tgggtagtta ccggaggagg
      181 cagagccggt cgcacgcctg ggtcccagcg gaggcccagt cgactctcga cccgggcccc
      241 agcctccttg cgtggcgaag gtcgtgcctc aggtctcctg gcgaacctgg ccgcccggaa
      301 gcggagagtg actcctcccc ttcgtcccct cggtgcccgc caggcggcgc cgtcggctgg
      361 gcccggattc ccctgcggct tcgatccctt tccactgggt attgagattg gcgaggggag
      421 aggcgcagaa acggcgtgat gcagaaagcc tcagtgttgc tcttcctggc ctgggtctgc
      481 ttcctcttct acgctggcat tgccctcttc accagtggct tcctgctcac ccgtttggag
      541 ctcaccaacc atagcagctg ccaagagccc ccaggccctg ggtccctgcc atgggggagc
      601 caagggaaac ctggggcctg ctggatggct tcccgatttt cgcgggttgt gttggtgctg
      661 atagatgctc tgcgatttga cttcgcccag ccccagcatt cacacgtgcc tagagagcct
      721 cctgtctccc tacccttcct gggcaaacta agctccttgc agaggatcct ggagattcag
      781 ccccaccatg cccggctcta ccgatctcag gttgaccctc ctaccaccac catgcagcgc
      841 ctcaaggccc tcaccactgg ctcactgcct acctttattg atgctggtag taacttcgcc
      901 agccacgcca tagtggaaga caatctcatt aagcagctca ccagtgcagg aaggcgtgta
      961 gtcttcatgg gagatgatac ctggaaagac cttttccctg gtgctttctc caaagctttc
     1021 ttcttcccat ccttcaatgt cagagaccta gacacagtgg acaatggcat cctggaacac
     1081 ctctacccca ccatggacag tggtgaatgg gacgtgctga ttgctcactt cctgggtgtg
     1141 gaccactgtg gccacaagca tggccctcac caccctgaaa tggccaagaa acttagccag
     1201 atggaccagg tgatccaggg acttgtggag cgtctggaga atgacacact gctggtagtg
     1261 gctggggacc atgggatgac cacaaatgga gaccatggag gggacagtga gctggaggtc
     1321 tcagctgctc tctttctgta tagccccaca gcagtcttcc ccagcacccc accagaggag
     1381 ccagaggtga ttcctcaagt tagccttgtg cccacgctgg ccctgctgct gggcctgccc
     1441 atcccatttg ggaatatcgg ggaagtgatg gctgagctat tctcaggggg tgaggactcc
     1501 cagccccact cctctgcttt agcccaagcc tcagctctcc atctcaatgc tcagcaggtg
     1561 tcccgatttc ttcataccta ctcagctgct actcaggacc ttcaagctaa ggagcttcat
     1621 cagctgcaga acctcttctc caaggcctct gctgactacc agtggcttct ccagagcccc
     1681 aagggggctg aggcgacact gccgactgtg attgctgagc tgcagcagtt cctgcgggga
     1741 gctcgggcca tgtgcatcga gtcttgggct cgtttctctc tggtccgcat ggcggggggt
     1801 actgctctct tggctgcttc ctgctttatc tgcctgctgg catctcagtg ggcaatatcc
     1861 ccaggctttc cattctgccc tctactcctg acacctgtgg cctggggcct ggttggggcc
     1921 atagcgtatg ctggactcct gggaactatt gagctgaagc tagatctagt gcttctaggg
     1981 gctgtggctg cagtgagctc attcctccct tttctgtgga aagcctgggc tggctggggg
     2041 tccaagaggc ccctggcaac cctgtttccc atccctgggc ccgtcctgtt actcctgctg
     2101 tttcgcttgg ctgtgttctt ctctgatagt tttgttgtag ctgaggccag ggccaccccc
     2161 ttccttttgg gctcattcat cctgctcctg gttgtccagc ttcactggga gggccagctg
     2221 cttccaccta agctactcac aatgccccgc cttggcactt cagccacaac aaacccccca
     2281 cggcacaatg gtgcatatgc cctgaggctt ggaattgggt tgcttttatg tacaaggcta
     2341 gctgggcttt ttcatcgttg ccctgaagag acacctgttt gccactcctc tccctggctg
     2401 agtcctctgg catccatggt gggtggtcga gccaagaatt tgtggtatgg agcttgtgtg
     2461 gcggcgctgg tggccctgtt agctgccgtg cgcttgtggc ttcgccgcta tggtaatctc
     2521 aagagccccg agccacccat gctctttgtg cgctggggac tgcccctaat ggcattgggt
     2581 actgctgcct actgggcatt ggcgtcgggg gcagatgagg ctcccccccg tctccgggtc
     2641 ctggtctctg gggcatccat ggtgctgcct cgggctgtag cagggctggc tgcttcaggg
     2701 ctcgcgctgc tgctctggaa gcctgtgaca gtgctggtga aggctggggc aggcgctcca
     2761 aggaccagga ctgtcctcac tcccttctca ggccccccca cttctcaagc tgacttggat
     2821 tatgtggtcc ctcaaatcta ccgacacatg caggaggagt tccggggccg gttagagagg
     2881 accaaatctc agggtcccct gactgtggct gcttatcagt tggggagtgt ctactcagct
     2941 gctatggtca cagccctcac cctgttggcc ttcccacttc tgctgttgca tgcggagcgc
     3001 atcagccttg tgttcctgct tctgtttctg cagagcttcc ttctcctaca tctgcttgct
     3061 gctgggatac ccgtcaccac ccctggtcct tttactgtgc catggcaggc agtctcggct
     3121 tgggccctca tggccacaca gaccttctac tccacaggcc accagcctgt ctttccagcc
     3181 atccattggc atgcagcctt cgtgggattc ccagagggtc atggctcctg tacttggctg
     3241 cctgctttgc tagtgggagc caacaccttt gcctcccacc tcctctttgc agtaggttgc
     3301 ccactgctcc tgctctggcc tttcctgtgt gagagtcaag ggctgcggaa gagacagcag
     3361 cccccaggga atgaagctga tgccagagtc agacccgagg aggaagagga gccactgatg
     3421 gagatgcggc tccgggatgc gcctcagcac ttctatgcag cactgctgca gctgggcctc
     3481 aagtacctct ttatccttgg tattcagatt ctggcctgtg ccttggcagc ctccatcctt
     3541 cgcaggcatc tcatggtctg gaaagtgttt gcccctaagt tcatatttga ggctgtgggc
     3601 ttcattgtga gcagcgtggg acttctcctg ggcatagctt tggtgatgag agtggatggt
     3661 gctgtgagct cctggttcag gcagctattt ctggcccagc agaggtagcc tagtctgtga
     3721 ttactggcac ttggctacag agagtgcttg agaacagtgt agcctggcct gtacaggtac
     3781 tggatgatct gcaagacagg ctcagccata ctcttactat catgcagcca ggggccgctg
     3841 acatctagga cttcattatt ctataattca ggaccacagt ggagtatgat ccctaactcc
     3901 tgatttggat gcatctgagg gacaaggggg gcggtctccg aagtggaata aaataggccg
     3961 ggcgtggtga cttgcaccta taatcccagc actttgggag gcagaggtgg gaggattgct
     4021 tggtcccagg agttcaagac cagcctgtgg aacataacaa gaccccgtct ctactattta
     4081 aaaaaaagtg taataaaatg ataatataat ta
//
