LOCUS       NM_176787               7936 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens phosphatidylinositol glycan anchor biosynthesis class
            N (PIGN), transcript variant 1, mRNA.
ACCESSION   NM_176787
VERSION     NM_176787.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Loong,L., Tardivo,A., Knaus,A., Hashim,M., Pagnamenta,A.T., Alt,K.,
            Bohrer-Rabel,H., Caro-Llopis,A., Cole,T., Distelmaier,F., Edery,P.,
            Ferreira,C.R., Jezela-Stanek,A., Kerr,B., Kluger,G., Krawitz,P.M.,
            Kuhn,M., Lemke,J.R., Lesca,G., Lynch,S.A., Martinez,F., Maxton,C.,
            Mierzewska,H., Monfort,S., Nicolai,J., Orellana,C., Pal,D.K.,
            Ploski,R., Quarrell,O.W., Rosello,M., Rydzanicz,M., Sabir,A.,
            Smigiel,R., Stegmann,A.P.A., Stewart,H., Stumpel,C., Szczepanik,E.,
            Tzschach,A., Wolfe,L., Taylor,J.C., Murakami,Y., Kinoshita,T.,
            Bayat,A. and Kini,U.
  TITLE     Biallelic variants in PIGN cause Fryns syndrome, multiple
            congenital anomalies-hypotonia-seizures syndrome, and neurologic
            phenotypes: A genotype-phenotype correlation study
  JOURNAL   Genet Med 25 (1), 37-48 (2023)
   PUBMED   36322149
  REMARK    GeneRIF: Biallelic variants in PIGN cause Fryns syndrome, multiple
            congenital anomalies-hypotonia-seizures syndrome, and neurologic
            phenotypes: A genotype-phenotype correlation study.
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Siavriene,E., Maldziene,Z., Mikstiene,V., Petraityte,G.,
            Rancelis,T., Dapkunas,J., Burnyte,B., Benusiene,E.,
            Sasnauskiene,A., Grikiniene,J., Griskeviciute,E., Utkus,A. and
            Preiksaitiene,E.
  TITLE     PIGN-Related Disease in Two Lithuanian Families: A Report of Two
            Novel Pathogenic Variants, Molecular and Clinical Characterisation
  JOURNAL   Medicina (Kaunas) 58 (11), 1526 (2022)
   PUBMED   36363484
  REMARK    GeneRIF: PIGN-Related Disease in Two Lithuanian Families: A Report
            of Two Novel Pathogenic Variants, Molecular and Clinical
            Characterisation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7936)
  AUTHORS   Teye,E.K., Lu,S., Chen,F., Yang,W., Abraham,T., Stairs,D.B.,
            Wang,H.G., Yochum,G.S., Brodsky,R.A. and Pu,J.J.
  TITLE     PIGN spatiotemporally regulates the spindle assembly checkpoint
            proteins in leukemia transformation and progression
  JOURNAL   Sci Rep 11 (1), 19022 (2021)
   PUBMED   34561473
  REMARK    GeneRIF: PIGN spatiotemporally regulates the spindle assembly
            checkpoint proteins in leukemia transformation and progression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7936)
  AUTHORS   De Giorgis,V., Paoletti,M., Varesio,C., Gana,S., Rognone,E.,
            Dallavalle,G., Papalia,G. and Pichiecchio,A.
  TITLE     Novel insights into the clinico-radiological spectrum of phenotypes
            associated to PIGN mutations
  JOURNAL   Eur J Paediatr Neurol 33, 21-28 (2021)
   PUBMED   34051595
  REMARK    GeneRIF: Novel insights into the clinico-radiological spectrum of
            phenotypes associated to PIGN mutations.
REFERENCE   5  (bases 1 to 7936)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   6  (bases 1 to 7936)
  AUTHORS   Kinoshita,T. and Inoue,N.
  TITLE     Dissecting and manipulating the pathway for
            glycosylphos-phatidylinositol-anchor biosynthesis
  JOURNAL   Curr Opin Chem Biol 4 (6), 632-638 (2000)
   PUBMED   11102867
  REMARK    Review article
REFERENCE   7  (bases 1 to 7936)
  AUTHORS   Hong,Y., Maeda,Y., Watanabe,R., Ohishi,K., Mishkind,M., Riezman,H.
            and Kinoshita,T.
  TITLE     Pig-n, a mammalian homologue of yeast Mcd4p, is involved in
            transferring phosphoethanolamine to the first mannose of the
            glycosylphosphatidylinositol
  JOURNAL   J Biol Chem 274 (49), 35099-35106 (1999)
   PUBMED   10574991
REFERENCE   8  (bases 1 to 7936)
  AUTHORS   Gaynor,E.C., Mondesert,G., Grimme,S.J., Reed,S.I., Orlean,P. and
            Emr,S.D.
  TITLE     MCD4 encodes a conserved endoplasmic reticulum membrane protein
            essential for glycosylphosphatidylinositol anchor synthesis in
            yeast
  JOURNAL   Mol Biol Cell 10 (3), 627-648 (1999)
   PUBMED   10069808
REFERENCE   9  (bases 1 to 7936)
  AUTHORS   Slavotinek,A.
  TITLE     Fryns Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301632
REFERENCE   10 (bases 1 to 7936)
  AUTHORS   Longoni,M., Pober,B.R. and High,F.A.
  TITLE     Congenital Diaphragmatic Hernia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301533
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC090396.9, FP340563.2 and
            AC090354.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_176787.4.
            
            Summary: This gene encodes a protein that is involved in
            glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The
            GPI-anchor is a glycolipid found on many blood cells and serves to
            anchor proteins to the cell surface. This protein is expressed in
            the endoplasmic reticulum and transfers phosphoethanolamine (EtNP)
            to the first mannose of the GPI anchor. Two alternatively spliced
            variants, which encode an identical isoform, have been reported.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer variant.
            Variants 1 and 2 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.91181.1, BC028363.2
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000640252.2/ ENSP00000492233.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-213               AC090396.9         21919-22131
            214-339             AC090396.9         45319-45444
            340-416             AC090396.9         46646-46722
            417-669             AC090396.9         47590-47842
            670-791             AC090396.9         51167-51288
            792-890             AC090396.9         51748-51846
            891-997             AC090396.9         54324-54430
            998-1122            AC090396.9         60637-60761
            1123-1253           AC090396.9         61874-62004
            1254-1370           AC090396.9         62950-63066
            1371-1411           AC090396.9         65629-65669
            1412-1471           AC090396.9         68496-68555
            1472-1564           FP340563.2         285-377
            1565-1620           FP340563.2         1062-1117
            1621-1699           AC090396.9         94336-94414
            1700-1882           AC090396.9         95659-95841
            1883-2022           AC090354.8         524-663
            2023-2122           AC090354.8         3412-3511
            2123-2215           AC090354.8         3616-3708
            2216-2307           AC090354.8         4863-4954
            2308-2416           AC090354.8         7595-7703
            2417-2525           AC090354.8         9314-9422
            2526-2628           AC090354.8         14547-14649
            2629-2731           AC090354.8         19919-20021
            2732-2818           AC090354.8         21655-21741
            2819-2874           AC090354.8         25233-25288
            2875-2950           AC090354.8         25891-25966
            2951-3024           AC090354.8         27751-27824
            3025-3067           AC090354.8         35676-35718
            3068-3120           AC090354.8         37772-37824
            3121-7936           AC090354.8         64518-69333
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.33"
     gene            1..7936
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="phosphatidylinositol glycan anchor biosynthesis
                     class N"
                     /db_xref="GeneID:23556"
                     /db_xref="HGNC:HGNC:8967"
                     /db_xref="MIM:606097"
     exon            1..213
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            214..339
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            340..416
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            417..669
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     CDS             449..3244
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="MCD4 homolog; phosphatidylinositol-glycan
                     biosynthesis class N protein"
                     /codon_start=1
                     /product="GPI ethanolamine phosphate transferase 1"
                     /protein_id="NP_789744.1"
                     /db_xref="CCDS:CCDS45879.1"
                     /db_xref="GeneID:23556"
                     /db_xref="HGNC:HGNC:8967"
                     /db_xref="MIM:606097"
                     /translation="MLLFFTLGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAR
                     RLVLFVADGLRADALYELDENGNSRAPFIRNIIMHEGSWGISHTRVPTESRPGHVALI
                     AGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPDILPMFAKGASGDHVYTYSYD
                     AKREDFGAQDATKLDTWVFDNVKDFFHHARNNQSLFSKINEEKIVFFLHLLGIDTNGH
                     AHRPSSRDYKHNIKKVDDGVKEIVSMFNHFYGNDGKTTFIFTSDHGMTDWGSHGAGHP
                     SETLTPLVTWGAGIKYPQRVSAQQFDDAFLKEWRLENWKRLDVNQADIAPLMTSLIGV
                     PFPLNSVGILPVDYLNNTDLFKAESMFTNAVQILEQFKVKMTQKKEVTLPFLFTPFKL
                     LSDSKQFNILRKARSYIKHRKFDEVVSLCKELIHLALKGLSYYHTYDRFFLGVNVVIG
                     FVGWISYASLLIIKSHSNLIKGVSKEVKKPSHLLPCSFVAIGILVAFFLLIQACPWTY
                     YVYGLLPLPIWYAVLREFQVIQDLVVSVLTYPLSHFVGYLLAFTLGIEVLVLSFFYRY
                     MLTAGLTAFAAWPFLTRLWTRAKMTSLSWTFFSLLLAVFPLMPVVGRKPDISLVMGAG
                     LLVLLLSLCVVTSLMKRKDSFIKEELLVHLLQVLSTVLSMYVVYSTQSSLLRKQGLPL
                     MNQIISWATLASSLVVPLLSSPVLFQRLFSILLSLMSTYLLLSTGYEALFPLVLSCLM
                     FVWINIEQETLQQSGVCCKQKLTSIQFSYNTDITQFRQLYLDDIRRAFFLVFFLVTAF
                     FGTGNIASINSFDLASVYCFLTVFSPFMMGALMMWKILIPFVLVMCAFEAVQLTTQLS
                     SKSLFLIVLVISDIMALHFFFLVKDYGSWLDIGTSISHYVIVMSMTIFLVFLNGLAQL
                     LTTKKLRLCGKPKSHFM"
     exon            670..791
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            792..890
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            891..997
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1122
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1253
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1370
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1411
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..1471
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1564
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1620
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1699
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1882
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..2022
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2122
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2215
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2307
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2416
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2417..2525
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2526..2628
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2629..2731
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2818
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..2874
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2875..2950
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3024
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            3025..3067
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            3068..3120
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     exon            3121..7936
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3737..3742
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="hexamer: AATAAA"
     polyA_site      3759
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="major polyA site"
     regulatory      4849..4854
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="hexamer: AATAAA"
     polyA_site      4876
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
     regulatory      6728..6733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="hexamer: AATAAA"
     regulatory      6740..6745
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="hexamer: AATAAA"
     polyA_site      6749
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
     polyA_site      6759
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
     regulatory      7914..7919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
                     /note="hexamer: AATAAA"
     polyA_site      7936
                     /gene="PIGN"
                     /gene_synonym="MCAHS; MCAHS1; MCD4; MDC4; PIG-N"
ORIGIN      
        1 gagaattatc gcgatagcag cagcggccgg gcaggtattg ggggcgttcc ggaattgagg
       61 ggtggttgta gtggtggctt aggaataccc tcccggctat cgcttcagtg ggtagagggg
      121 accactgccc gagagcttta atggagctgg gtcctgcctt cgcgctgagg agccctcgtt
      181 ttcgagatca ggcctgaccg ggataagctc cagaaacagt attatgtctc agtcctgtgc
      241 aaagtgagat gagctgtagc ctttggagac ctataatttt gaacatagat gttccttaaa
      301 agctaaatct ttttgaacag atgatgataa acctggaaga gattcaccaa caatagcaaa
      361 ttacacactg gcttggaaag tactcccatg ttccgtttga tcttggcaat ttaatgctgt
      421 tcttgaagac taattagtta cactggatat gctgctgttc tttactttgg gattgcttat
      481 acattttgtg ttcttcgcct ccatctttga catttatttt acatctcctt tggttcatgg
      541 aatgactcct cagtttacac cattgcctcc tccagcgaga agattagtgt tgtttgttgc
      601 tgatggcctt cgagcagatg cactttacga attagatgaa aatggaaact ctagagcacc
      661 gtttattagg aatatcataa tgcatgaagg cagctggggc atatctcata cacgtgtgcc
      721 aacagaatct cggccaggtc atgtagctct gatagctggg ttttatgaag atgtcagtgc
      781 agttgccaaa ggatggaagg aaaatcctgt agagtttgat tctcttttta atgaaagtaa
      841 atacacatgg agctggggaa gcccagatat cctgcctatg tttgccaaag gtgctagtgg
      901 agaccacgtt tatacatata gttatgatgc taaaagagag gattttggtg ctcaagatgc
      961 aacaaaactg gatacgtggg tttttgataa tgttaaggac ttctttcatc atgccagaaa
     1021 caaccagtct ttgttttcta aaataaatga agagaaaata gtttttttct tacatttatt
     1081 aggaatagat acaaacggac atgctcatcg accatcctcg agagactaca agcacaatat
     1141 taaaaaagtt gatgatggag ttaaagaaat cgtgtctatg tttaaccact tctatggaaa
     1201 tgatgggaaa acaacattta tctttacctc tgaccatgga atgacagact ggggttccca
     1261 tggggctggt catccttcag agactttaac tcctttagtc acttggggag ctggaatcaa
     1321 gtatccccaa agagtatcag ctcagcaatt tgatgatgca tttttgaaag agtggagatt
     1381 ggagaattgg aagaggctag atgtcaatca ggctgatatt gcaccattga tgacttccct
     1441 tattggagtt ccctttcctc ttaactcagt gggaatcctt cctgtggatt atcttaacaa
     1501 cactgatctc ttcaaagcag agagcatgtt tacaaatgca gtacagattc ttgaacagtt
     1561 caaggtgaaa atgactcaga agaaagaagt tactttacca tttttgttta caccatttaa
     1621 actgctttct gattccaaac agttcaacat tttaagaaaa gcaagatctt atataaaaca
     1681 cagaaagttt gatgaagtgg tctccctttg caaggagcta attcatcttg cattgaaagg
     1741 attgtcctat tatcacacat atgacagatt ctttttgggc gtcaatgttg ttattggttt
     1801 tgtgggatgg atatcttatg cctctttgtt gatcatcaag tctcattcca accttataaa
     1861 aggtgttagt aaagaagtga agaaaccaag ccatctcctg ccttgtagtt ttgtagctat
     1921 tggcatttta gtagcatttt ttctgctgat tcaagcctgt ccctggacat attatgtata
     1981 tggtttgttg ccactgccaa tatggtatgc ggttctaaga gaatttcaag ttattcagga
     2041 ccttgttgta tcagtgttga cctatcctct gagccatttt gttgggtacc tgttagcctt
     2101 taccctggga attgaagtat tagttctcag ttttttctac cgctatatgc ttaccgctgg
     2161 acttactgcc tttgcagctt ggccatttct cactcggctg tggactcgag caaagatgac
     2221 ctcactgagt tggactttct tctctttgct cctggcagtg ttcccactga tgccggttgt
     2281 aggtcgaaag ccagacatct ctctagtgat gggtgcaggc ttgctggttc ttctgttatc
     2341 cctgtgtgtt gtaacatctc tcatgaaaag aaaagatagc tttataaagg aagagctatt
     2401 ggtacatctg ttacaggtgc tgagcacagt gctctccatg tatgttgtgt atagcactca
     2461 gagtagtcta ctcaggaagc aaggactgcc tctcatgaat caaattatta gctgggcaac
     2521 attagcctct tccttggttg tgccactact gagttctcca gttctctttc agcgattgtt
     2581 cagcatactt ctttcattga tgtcaaccta cctacttcta agcacagggt atgaagctct
     2641 ctttccacta gtgttgtctt gtttgatgtt tgtctggata aacatagaac aagaaactct
     2701 acaacaatct ggtgtttgct gtaaacaaaa gctcaccagt atccagttct cttataatac
     2761 tgatataact cagtttcgac agctatatct ggatgacatc cgtagggcct ttttccttgt
     2821 tttcttctta gtgacagcat tttttggaac tggaaatata gcttctatta acagctttga
     2881 tcttgcctct gtctattgct ttctgactgt gttcagtcct tttatgatgg gagccctgat
     2941 gatgtggaag attttaatcc cctttgttct tgttatgtgt gcttttgaag cagttcagtt
     3001 gactactcag ttatcgtcaa aaagcctttt tctcattgtt ctcgtcatat cagacattat
     3061 ggctttgcat tttttcttct tggtcaagga ttatggcagc tggcttgata ttgggacaag
     3121 catcagccac tatgtgattg tcatgtccat gaccatcttt ttggtgttcc tcaatggcct
     3181 ggcccagctg ctcacaacga agaaactcag actatgtggc aaacccaaaa gtcacttcat
     3241 gtgaggttgc tgaagcacca ttcagcatct ggatcctgat tctcctttta agctaaaatc
     3301 tcatcaaggc ttcaataaga agatggatat ggatatatag tatattctac tcctgtaagg
     3361 aaaatggtat ttggaattcc gaattgacag gttatctgga acaaaggagc ttcttttttt
     3421 ttctaggttt tgcaggcatg aaatagtgat tatatctgtg gaaaagcata ggaaggcatt
     3481 ctcctttttc atttttttcc tttggctggc agctcttccc agtgatgttg agagcacctg
     3541 cagcaatctg gtccccagtc gcacaacttc ccacataccc agaggagagc atatgcctgt
     3601 gggggcagtg ctgatggcat ccagagtcat tgctgtggct gagctggaag gaaatcacca
     3661 ggtgccaccg tcaatattta tcagctttca gcactggttt tgttagacag tcagggtgta
     3721 ttatttcaag agcttcaata aaaaaagtgt ttgcaggtat ttgtcatatt tctttagtat
     3781 gcaataatgc tatcttatcc tttttagtgc tatttctcag gttttctcag tcaaccagct
     3841 agatgcatgt gatgcatgtc tatccttcac tttttttttt tcatttctgg attttcctgg
     3901 gatttctgac aacacaaaag tagaaagtgc ttggtatttc tgtcttatac ataggaaaac
     3961 atttaaaata aatagttttt aattgtaggc tcaacatctg ttaccccata gctttttcgt
     4021 atattgtttc tttccctttg aatttctttt tactgtcatc tattatttta acaggagtta
     4081 aaataactcc tgttaaactt tctgacaata tcataagaat tcatattaaa ggtgttattc
     4141 tttctggttg tgagattcta gcacaaaggc ctaaaatcat caaattagat ttccatttgt
     4201 aaacaaattc taatgggcaa aatatcttga gagcagtttt tatatggatt atccataaaa
     4261 tgacctttac atatccctgc agagttggcc ttttgcccgt ggtgtgctag tagctttggc
     4321 tgatgctaag ctttcctggt atgcgcccta tttttaagaa gtaattgctt ttgaattaag
     4381 ttatagcatt actaattcat gttaatgact aggaaaccct ctgtaattta caagattttt
     4441 caaattggtg gggagtgaat aaatacaatt taaaagtcag aaatcagttt ggcaaagtgt
     4501 actttcttaa tttctattta tgatgaagta tagtcataat ttatttgtaa tactacttta
     4561 tggtatacca gtgaaagaac tgtagtataa aaaagaggta ttaatgtttt atgaaatctc
     4621 atgcatcagt tcatagcata aaatctagct ggacaactaa gaagctatgg tagcaaacag
     4681 tgatgttgat ggaatgagaa tcatgaactt tcatattacc tcaaaggatt tttttatcag
     4741 tttttttcac acatcagaaa aaactgactg tataaacact tatcactgac ctttttctat
     4801 gtgtagtttt gccttttatc ttttcccaaa tttttataaa gagaaattaa taaatatttt
     4861 attacaacat tgtaaagggt gctcagtttt tttatctttc tttattttgc tctttgaggc
     4921 atatcttttt cttaattttg tcctttcagg tcagtgtgag tctgtagaat aggttgaact
     4981 tccttctgac agtatcatag agtttgtgga gtttgtgttg tgtgcctgca gccacaggct
     5041 cgccttgctg caccgtaact ggccccttca gactcacatg ggaacaagcc ttttctgttg
     5101 cagtttccca gatatcttta aatgttttga aatgtatatt tttatgttgc aaaacagaaa
     5161 aaagaaacga gttagggaaa aagtatttct atttaaggta tttatttttt cattctacct
     5221 atgccataga tttctgaatc aagacttagg acatttgctg attaacaatg tttaaattag
     5281 gaattgttat aagaattaaa ataggcagaa caagttgtga aaatattgaa ccaaaaaaag
     5341 gccatgctat catcttagag ggcccatgaa taaaccttag agggaatcct aaggctttag
     5401 gaaaccgctg cagcttgtct gtcattttcc aatatgttaa ttattatact tagtgtacag
     5461 tatttagaat acaaaatgct tttttatttt ttaggaaaat aatgttataa atgattgtat
     5521 attttcatag taatgtcttt ataattattt attaatgata gagctgaatt agaaaagctc
     5581 ttcatccatt ataatatggt ttctcatgaa aaacttatta caaatttcag ccaactcaga
     5641 tattaagaac atcagtgtca atcctcctcc cctcctgcga cctttttaca cttgttttgg
     5701 tggactaggg gctccttttc tcctcctttt tgacatataa ctgtgccatt aaaaacatga
     5761 tttctgatga agctggccta gagggaatga aggagattgc gtgggtatgg tctctaggac
     5821 aggcgtagct gttgcagggc caccctgtcc ttgggagaac ctggtgagct cccatattca
     5881 cactagatgc catcatgtca gggggctgaa gagaatggag ctaatcaact ctgtcatttg
     5941 gagaagaccc ccttcagacc agttctgaga actccttctt ttaagagacc agagataact
     6001 tgatcatttc tcaccttttc gtagttgttc ttttttatgt tgacattccc cttactgttt
     6061 ttaaaatgct tcaaagcatt aatgattgtt atgctgtgtg ctttcagtac tatggcaata
     6121 tctttgtagc attttcccat ttctgtataa gcatggtttt ttgttttttt ttttttcgtt
     6181 tttgttttga gacagggtct cactcttgtt caggctagaa tgcagtggca ccatctcagc
     6241 tcactgcaac ctctgcctcc tgggctcaag tgatcctgcc acctcagcct cctgagtagc
     6301 tgagactaca ggcacatgcc cccacaccta gctaatttta aaatattttt tgatggagac
     6361 agggtttcac tatgttttcc aggcagcagc attttttttt gactgggatt tctttatgat
     6421 gctttgttat ttaaaaaaaa aatgagaagg aaacattaat aatcaaaatt atctttaatt
     6481 ggcctcacag ggctaaaatc tagaattatt attactcttg ggtaaactca tgaaaagtac
     6541 ttaatattca ttctataaag aaacaaaaag aacctttgag atgtgtctct tattcatttc
     6601 cttgggtaca cttaaaacgg gttacagtta ctgctcaact ttctaccttc agaactgcag
     6661 catttatttg ccacacagca gcatttatca gagattgtcc cttagaacat tggttccttg
     6721 taatacaaat aaatgtcata ataaaaatag tttctttttt ctttctttct ttttttttca
     6781 agacagactc tggctctctc gccaaggctg gaatgcagtg gcgcaatgtc agctcactgc
     6841 aagctccgcc tccctggttc aagcgattct tctgcctcag cctcctgagt agctgggatt
     6901 acaggtgccc accaccacgt ctggctaatt ttcatatttt tagtactgac aaggtttcac
     6961 catgtttgtc aggctcatct tgaactcctg acctcaagca gttcacccgc ctcggcctcc
     7021 caaagtgctg ggattacagg cgtgagctgc ctcacccagc tgaaaagagt ttcttaatta
     7081 aatatatttg agaaacatct agtcaaacca ccttaaatag gtatatttac attagttcta
     7141 agggaattta acttgtgatg tacgttgtga agggaggaag atagcattat gccaaaagtt
     7201 tccactagct tgacagcaga ccctgtctta gagttttctt cctgattggt aaacataaga
     7261 aatgtttcca aagccgggct tggtggctca cccctgtaat tccagcactt tgggaggccg
     7321 aggtgggtgg atcacttgag gtcaggagtt cgagaccagt ctggccaaga tggcaaaacc
     7381 ccatctctac taaacacaca cacacacaca cacacacaca cacacacaca cacacacaca
     7441 cacacacaca cacacacacc cggcggggtg gtaggctcct gtaatccttg ctactcagga
     7501 gcctgaggca tgagaattgc ttgaacctgg gaggtggaat ttgcaatgag ccgagatggt
     7561 gccactctgg gcgacaaagt gagactctgc ctcaaaaaga aatgtctcca tataattttg
     7621 cactaaaacc ttaatttgtc atagttttga aaatagggtg ttgtatgttg attgaatttt
     7681 tttccctgag aactggaata ccatttttac tttaaccctt aatgtgagaa aactttacaa
     7741 tttcctgcaa tttttcctgc ctctgtatat attacacctg tcatcattta gtctttcatg
     7801 tgggaaaggc cagtggtacc agattgcatt cggttcatca tggctcattg tctagccctg
     7861 ctagagtata gactaagcct tcatacctta tgttttatat cttttacctg aaaaataaat
     7921 ttgcaccaaa tttcaa
//
