LOCUS       NM_145167               7038 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens phosphatidylinositol glycan anchor biosynthesis class
            M (PIGM), mRNA.
ACCESSION   NM_145167
VERSION     NM_145167.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Eisenhaber,B., Sinha,S., Wong,W.C. and Eisenhaber,F.
  TITLE     Function of a membrane-embedded domain evolutionarily multiplied in
            the GPI lipid anchor pathway proteins PIG-B, PIG-M, PIG-U, PIG-W,
            PIG-V, and PIG-Z
  JOURNAL   Cell Cycle 17 (7), 874-880 (2018)
   PUBMED   29764287
  REMARK    GeneRIF: Function of a membrane-embedded domain evolutionarily
            multiplied in the GPI lipid anchor pathway proteins PIG-B, PIG-M,
            PIG-U, PIG-W, PIG-V, and PIG-Z has been described.
REFERENCE   3  (bases 1 to 7038)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   4  (bases 1 to 7038)
  AUTHORS   Costa,J.R., Caputo,V.S., Makarona,K., Layton,D.M., Roberts,I.A.,
            Almeida,A.M. and Karadimitris,A.
  TITLE     Cell-type-specific transcriptional regulation of PIGM underpins the
            divergent hematologic phenotype in inherited GPl deficiency
  JOURNAL   Blood 124 (20), 3151-3154 (2014)
   PUBMED   25293775
  REMARK    GeneRIF: B cells but not red cells are glycosylphosphatidylinositol
            deficient in PIGM-associated inherited glycosylphosphatidylinositol
            deficiency, caused by a core promoter mutation that abrogates Sp1
            binding.
REFERENCE   5  (bases 1 to 7038)
  AUTHORS   Freeze,H.H.
  TITLE     Understanding human glycosylation disorders: biochemistry leads the
            charge
  JOURNAL   J Biol Chem 288 (10), 6936-6945 (2013)
   PUBMED   23329837
  REMARK    GeneRIF: Studies indicate that mutations in PGM1 by exome
            sequencing cause hypoglycemia and liver abnormalities.
            Review article
REFERENCE   6  (bases 1 to 7038)
  AUTHORS   Almeida,A.M., Murakami,Y., Layton,D.M., Hillmen,P., Sellick,G.S.,
            Maeda,Y., Richards,S., Patterson,S., Kotsianidis,I., Mollica,L.,
            Crawford,D.H., Baker,A., Ferguson,M., Roberts,I., Houlston,R.,
            Kinoshita,T. and Karadimitris,A.
  TITLE     Hypomorphic promoter mutation in PIGM causes inherited
            glycosylphosphatidylinositol deficiency
  JOURNAL   Nat Med 12 (7), 846-851 (2006)
   PUBMED   16767100
  REMARK    GeneRIF: Mutation substantially reduces transcription of PIGM and
            blocks mannosylation of glycosylphosphatidylinositol(GPI), leading
            to partial but severe deficiency of GPI.
REFERENCE   7  (bases 1 to 7038)
  AUTHORS   Maeda,Y., Watanabe,R., Harris,C.L., Hong,Y., Ohishi,K.,
            Kinoshita,K. and Kinoshita,T.
  TITLE     PIG-M transfers the first mannose to glycosylphosphatidylinositol
            on the lumenal side of the ER
  JOURNAL   EMBO J 20 (1-2), 250-261 (2001)
   PUBMED   11226175
REFERENCE   8  (bases 1 to 7038)
  AUTHORS   Kinoshita,T. and Inoue,N.
  TITLE     Dissecting and manipulating the pathway for
            glycosylphos-phatidylinositol-anchor biosynthesis
  JOURNAL   Curr Opin Chem Biol 4 (6), 632-638 (2000)
   PUBMED   11102867
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX643162.1, BC019865.1 and
            AL513302.15.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_145167.2.
            
            Summary: This gene encodes a transmembrane protein that is located
            in the endoplasmic reticulum and is involved in GPI-anchor
            biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a
            glycolipid which contains three mannose molecules in its core
            backbone. The GPI-anchor is found on many blood cells and serves to
            anchor proteins to the cell surface. This gene encodes a
            mannosyltransferase, GPI-MT-I, that transfers the first mannose to
            GPI on the lumenal side of the endoplasmic reticulum. [provided by
            RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK074655.1, BC001803.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368090.5/ ENSP00000357069.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                BX643162.1         13-44
            33-2098             BC019865.1         1-2066
            2099-4319           AL513302.15        37382-39602         c
            4320-7038           AL513302.15        34663-37381         c
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.2"
     gene            1..7038
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="phosphatidylinositol glycan anchor biosynthesis
                     class M"
                     /db_xref="GeneID:93183"
                     /db_xref="HGNC:HGNC:18858"
                     /db_xref="MIM:610273"
     exon            1..7038
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /inference="alignment:Splign:2.1.0"
     CDS             252..1523
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /EC_number="2.4.1.-"
                     /note="PIG-M mannosyltransferase; GPI mannosyltransferase
                     I; phosphatidylinositol-glycan biosynthesis class M
                     protein; DPM:GlcN-(acyl-)PI mannosyltransferase; dol-P-Man
                     dependent GPI mannosyltransferase"
                     /codon_start=1
                     /product="GPI mannosyltransferase 1"
                     /protein_id="NP_660150.1"
                     /db_xref="CCDS:CCDS1192.1"
                     /db_xref="GeneID:93183"
                     /db_xref="HGNC:HGNC:18858"
                     /db_xref="MIM:610273"
                     /translation="MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLH
                     VRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISC
                     DLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYL
                     IKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYEL
                     LKRLCNRAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFY
                     MLYLTAESKWSFSLGIAAFLPQLILLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQ
                     YFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLA
                     GLFFLLINCSILIQIISHYKEEPLTERIKYD"
     regulatory      1748..1753
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="hexamer: AATAAA"
     polyA_site      1776
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="major polyA site"
     regulatory      3903..3908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="hexamer: AATACA"
     polyA_site      3925
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="major polyA site"
     regulatory      6950..6955
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="hexamer: AATAAA"
     polyA_site      6979
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="major polyA site"
     regulatory      7016..7021
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="hexamer: AATAAA"
     polyA_site      7038
                     /gene="PIGM"
                     /gene_synonym="GPI-MT-I"
                     /note="major polyA site"
ORIGIN      
        1 ggaagcgaga ccgtccatcc agaggaaggc aagtttttgg ctcgggcggc tgagaagacc
       61 gcgcggggct ggagacaggt agcagtacgg gggcggggct tcatgccgga tgtgatagtc
      121 tgcagtcgtt tcggttggca gcctggcggg tgggagatgc ggcggccacc tgctgcaaag
      181 aaccgaaggg aaggttagaa gtacgaaggc agtttggagc tggggctaag cagctgtcgc
      241 acggtcagat catgggctcc accaagcact ggggcgaatg gctcctgaac ttgaaggtgg
      301 ctccagccgg cgtctttggt gtggcctttc tagccagagt cgccctggtt ttctatggcg
      361 tcttccagga ccggaccctg cacgtgaggt atacggacat cgactaccag gtcttcaccg
      421 acgccgcgcg cttcgtcacg gaggggcgct cgccttacct gagagccacg taccgttaca
      481 ccccgctgct gggttggctc ctcactccca acatctacct cagcgagctc tttggaaagt
      541 ttctcttcat cagctgcgac ctcctcaccg ctttcctctt ataccgcctg ctgctgctga
      601 aggggctggg gcgccgccag gcttgtggct actgtgtctt ttggcttctt aaccccctgc
      661 ctatggcagt atccagccgc ggtaatgcgg actctattgt cgcctccctg gtcctgatgg
      721 tcctctactt gataaagaaa agactcgtcg cgtgtgcagc tgtattctat ggtttcgcgg
      781 tgcatatgaa gatatatcca gtgacttaca tccttcccat aaccctccac ctgcttccag
      841 atcgcgacaa tgacaaaagc ctccgtcaat tccggtacac tttccaggct tgtttgtacg
      901 agctcctgaa aaggctgtgt aatcgggctg tgctgctgtt tgtagcagtt gctggactca
      961 cgttttttgc cctgagcttt ggtttttact atgagtacgg ctgggaattt ttggaacaca
     1021 cctactttta tcacctgact aggcgggata tccgtcacaa cttttctccg tacttctaca
     1081 tgctgtattt gactgcagag agcaagtgga gtttttccct gggaattgct gcattcctgc
     1141 cacagctcat cttgctttca gctgtgtctt tcgcctatta cagagacctc gttttttgtt
     1201 gttttcttca tacgtccatt tttgtgactt ttaacaaagt ctgcacctcc cagtactttc
     1261 tttggtacct ctgcttactg cctcttgtga tgccactagt cagaatgcct tggaaaagag
     1321 ctgtagttct cctaatgtta tggtttatag ggcaggccat gtggctggct cctgcctatg
     1381 ttctagagtt tcaaggaaag aacacctttc tgtttatttg gttagctggt ttgttctttc
     1441 ttcttatcaa ttgttccatc ctgattcaaa ttatttccca ttacaaagaa gaacccctga
     1501 cagagagaat caaatatgac tagtgtatgt tccacaccct ctgctactgt gttacattct
     1561 gattgtcttg tatggaccag aagagagctt tgggacattt tttctgaaca ttctaagcat
     1621 tctagtgaaa gttcccatgt tccaacagaa cttaaaagca atgtttgcct tatatataaa
     1681 agggacacaa taattgaggt ccaccttcta ggaaatccta ggactcgttt atttgggaca
     1741 tggtgggaat aaaggtcaca tattggaaaa tggaaaggct gatgaaacta tcagatacta
     1801 aaacattctt aaaatagagg aatatagtta gagacatcag gtttaagcca gtatttgttc
     1861 ctgttttaca atgcttctgt cttaagctgt gtcttaactt ttaacaccca tcttttcttt
     1921 ctaaagcttt cctgacagct gtgaaaatcc aaaaaatatt cttaaactgt gtatggtggc
     1981 ccttgcctgt agtctcagca ctttgggagg ctgaggtggg agggtcgctt gagttcagga
     2041 gttctagacc cacctggggc aagatggtga gacctagtct caaaaaaaaa aaaaaaaatt
     2101 agccaggtgt tgtggtgcac ccctgtagtc atagctgcat gggaggctga ggtgggagaa
     2161 ttgcttgagc ccagagcaag accctgtctc aaaaaaaaaa aaaaaaaaaa aaaggaaagg
     2221 acaacttttt agatagaaaa gtattaaata atactaagat gcttagtagt attattttag
     2281 agagttttaa acttctatat taaatgtggg gtcttacaag ataatccaaa gactttggga
     2341 ggccaaggcg ggcagatcac gaggtcagga gattgagacc atcctggcta acacggtgaa
     2401 acccctctct actaaaaata caaaaaatta gccgggcgtg gtgggtgcct gtagtcccag
     2461 ctactcctcg ggaggctgag gcaggagaat ggcgtgaact ccggaggtgg agcctgtagt
     2521 gagccgagat ggcaccactg cgctccagcc tgggcgacag agcaagactc catatcaaaa
     2581 aaaaaaaaaa aaaaagataa tccaaagaat ttaaattgta atcatgtttc atgtatttgt
     2641 tttattactt acttttatag cacttagtcc cagtggtatt agactgctat ttggtttcat
     2701 acaaaaagga ttaaatttaa attcattcat gtttagactt gagttattac atttttaaaa
     2761 ctatcatctt gcctttaatg tttgtggtcc tacacaaact attagtacat ttcagtatcc
     2821 tcttacccct ttgtttttaa gtttttgatt gctaaagcaa gacttttttc ttctagaatt
     2881 taagtcaacc aagtgttatc tatgttgtaa aaatggataa tagtagattt taggtgataa
     2941 aacaacttgt tagtaagaca tttcctagct taaaaaaaaa aatcaaaaat tccatgatag
     3001 aaatgcagac ctgtgaggga aactcctgaa aagcataaga agcatcccag agagccatgg
     3061 gttttctaga ccagagaatt tagagggaga ttgtggaact gaggcttagg tggtcagatc
     3121 gtttccctta tcactgtaat atttctgggg gaaaaatgct ttctgagttg tttaaacaag
     3181 catccttaca tttttttttt taattaaaca gcctgtctag gcttgggatt ccctaatact
     3241 acagtagcag tatatgaata tgattttgtg attgtgtttt ttaaaagata agtaatttga
     3301 tgaactgttc ttttgcagtc agaaaacact cacaaaaaga caaaaaaagt tccacagtat
     3361 tatatttcat gtcagttcag gcctaaaatc ctttgcaaat aagatgttta taggctggtc
     3421 acaattaaca atgttattat tggcagcact tcttggatgg ataccttttg ggacctttca
     3481 ttagaaagag ggaaagaatg gggtggtttt gtatgggctc ctgtttgggg taaaaatagc
     3541 agagtcagtt gctgaggaca atgaccttcc ttataacatt tagtttcata cccatattag
     3601 gtcttgtctt gaggaccctt tatatgtgct tgtttactag tggccttcca gccatagcat
     3661 tcttaccttt ttttcctatt ctaagaatta aaaaaaaaaa ttatagagcc agcaagggag
     3721 gaggcaggaa acagaaatcg aatttcatca ttccagtata gttgtccctt tttttgtatt
     3781 tctgacttgg ttttataatt atatttactt actaattatt gttttttaac attctttatt
     3841 gtggcttact cttcatactt agaattgaaa ttgttggaca tcacatgtat attcacatta
     3901 taaatacatc attcttccac tgttagacct ttagattgct tccagttttt aatattctaa
     3961 ataagacttt caacattttc tgtgttttag ctcattctct taggacattc ttagaagtta
     4021 gaaacatttc tgctgggatc gttggaggga acttcaaact ttggaatctt tcctgcaaga
     4081 aattctttac caaagaaagg caggtgtttc ttaaagggat gcaaaagata ttttgcactt
     4141 tgtatgttcc aaaacattta gtaagttaac taaaaaaatg agttaatttg gtttcttggg
     4201 ggattttaat tttttaattt gttttctggt tatgtaaaaa aaatgttttt tttttgcttc
     4261 ttatcacaat ccttttgttt cctttttaat cctttaataa caccttcaaa ttttataaga
     4321 ctttggctta tttcctatat aattcttttt ttcttatacc acctcttaag attgatatgt
     4381 tcatttgcag gtaagcatta attattagat aaagagggat gattctcaag attgtgtgtg
     4441 ttctgaacag agggaactac atgacatttt cttctgtaat tgcctttgta acgtctttag
     4501 aatgtggttc ctaaatattc ctggataaat tctcttgata ggcccattgg aaaggctaat
     4561 actcccaccc agtgctttgt tccttcctgg caaaagaatt cctaaaacca ctgattttag
     4621 ttactgactt ctcaccatct ggactcttac aagatgtttc agaagttgtg tagaacttgt
     4681 ctttcagttg acttgtggct gaatttactg ttacttctct aatatcagtt gttttctgca
     4741 ttaccaccct ctcccctaac catctgtact atgaatggaa aaggaaaaag atggaaaaat
     4801 tatacctagg attgtcccta aatgcaacct cttggttccc cccacccctc atgttttatt
     4861 ataaacgatt ttaagagctg ggcatggtgg ttcatgccta taatcccaat gctttgggaa
     4921 gctgaggcag gaggatcact tgaggacagg agtttgagac cagcctggac aacatagtga
     4981 gacccccatc tgtacaaaaa aaaaagtgct acttgggaag ctgaagtggg aggaccactt
     5041 gagcccagga attcgaggag gttatagtga actatgattg tgccactgca ctccagcctg
     5101 ggtgacagag caacaccttg cctctaaaac aaacagctcc aactatttta tttttttatt
     5161 tttctgagac aagatgttgc tttgtcaccc aggctggagt gcagtagtat aaacatggct
     5221 cactgcagcc gaactcccag gctcaagtga tccttcctcc tcagcctccc aagtagctgg
     5281 gaccacaggt atttgccacc atgcctggct aatctttatt ttttgtagag acgaggtctt
     5341 gctatgttgc aagagggaga tcacttaagt gatcttccct cattggcttc ccaaagtgtt
     5401 gggattccag gcgtgagcca ctgcaccccg cctgacctaa ctattttaaa cactactcat
     5461 attgccatca tctaaattca acaacaattt gccatatttg ctttatgtat atgtataatt
     5521 atatatttat aaaatttttt gaaccatgtg aagttgcaaa catcattgaa cttcaccact
     5581 aaatatatca gcatgcatct cctaaaaatc aggtattttc ttacaaaacc ataattctgc
     5641 tatctattat gatttaacat tctgtcattt tcaaatttct tcaggtgttt tttgttacat
     5701 cttaaagaac agacgttctt ggatctcaaa gattccgagg aaggaaagaa catggtggat
     5761 aatccataat taagaagttt gaatcttttc ctagtcttaa aaacaaagtg agaactaaag
     5821 gggtttacct tccatcaaag ttgaacaaat taattctttt gtgtgtcata ctttctgtct
     5881 ctctccctaa aaacatgggg gtggtaatct ctttgtttta tctggtaggt tttccagata
     5941 aaagattata caggtttgga atcttaatat ccaaccccca actccaagcc ctgccccaac
     6001 aacaacagct aattatttta catcttgatg atggtaaatt tttcatgatt aaccaatttt
     6061 gggcacctat tccgccatat atggttttat ttaattttca cagcaacact tcaaagttga
     6121 tattcatatt ctaaatttat agatgaacga agaggctcaa agattaaaca acttgctaat
     6181 aagtgatttt tacccatgct ttttccatta tattatgttt tggatttttc cagagtaccc
     6241 aaacccagca gatgcatact gctccaaaat aaatggaact caatactggc tctgtcaagt
     6301 gtctcttgcc aatcaattgt ccttctaatc ttttgagggg cagttctctc agttgtacca
     6361 agtcactgtc atccccaaac ttttcaatga ttccaggcct ttaacaactc ccctccacct
     6421 ccaaaccctc tctcaccaaa ttgtgtaatt tctttgttta ctgctggctt catcatgcac
     6481 ttttccttca tagcctattt taagaagttg atttgctgaa ctgcttttta gccaagctat
     6541 ttgtaaatca agctacacaa agtatgtggc cataatttcc aaaaggcaaa tgatcattgt
     6601 tcaattgctg ttgctctgca gtgtgcattc atgcagttaa aaattgtact gcattgatag
     6661 tacggcatca gagaatagat cacttaggtt caaatcccag tgcatgatcc tgagcaagat
     6721 tatcatatct agatacatta gtttttatct gtgaaatggg aatgatagca caatcttcat
     6781 agaattgttg gtaaggatta agtgagttga tatgctcaaa gtgtgcatgc tggcatagaa
     6841 caagtcactg tttacaagcc tttaaagaag gagctgttct ggcactgtaa acttgaacct
     6901 tttttcccca aatctaatgg atataggcaa ggaaattata tttatataaa ataaatgttt
     6961 gactaccttt gatcataaac tttattctca tcttgacctg ttcctttgaa aagataataa
     7021 atactgatat gtgaaaaa
//
