LOCUS       NM_001198784             868 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens piercer of microtubule wall 2 (PIERCE2), mRNA.
ACCESSION   NM_001198784 XR_109198 XR_111527 XR_114855
VERSION     NM_001198784.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Gui,M., Croft,J.T., Zabeo,D., Acharya,V., Kollman,J.M.,
            Burgoyne,T., Hoog,J.L. and Brown,A.
  TITLE     SPACA9 is a lumenal protein of human ciliary singlet and doublet
            microtubules
  JOURNAL   Proc Natl Acad Sci U S A 119 (41), e2207605119 (2022)
   PUBMED   36191189
REFERENCE   2  (bases 1 to 868)
  AUTHORS   Gui,M., Farley,H., Anujan,P., Anderson,J.R., Maxwell,D.W.,
            Whitchurch,J.B., Botsch,J.J., Qiu,T., Meleppattu,S., Singh,S.K.,
            Zhang,Q., Thompson,J., Lucas,J.S., Bingle,C.D., Norris,D.P., Roy,S.
            and Brown,A.
  TITLE     De novo identification of mammalian ciliary motility proteins using
            cryo-EM
  JOURNAL   Cell 184 (23), 5791-5806 (2021)
   PUBMED   34715025
REFERENCE   3  (bases 1 to 868)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC013355.7, AV735277.1, AK130862.1 and BX648577.1.
            
            On May 17, 2019 this sequence version replaced NM_001198784.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.567899.1, AK130862.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000569691.2/ ENSP00000456337.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC013355.7         76142-76171
            31-53               AV735277.1         1-23
            54-780              AK130862.1         1-727
            781-782             BX648577.1         606-607
            783-868             AC013355.7         86360-86445
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.3"
     gene            1..868
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
                     /note="piercer of microtubule wall 2"
                     /db_xref="GeneID:145788"
                     /db_xref="HGNC:HGNC:44654"
                     /db_xref="MIM:619669"
     exon            1..292
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
                     /inference="alignment:Splign:2.1.0"
     CDS             275..640
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
                     /note="uncharacterized protein C15orf65"
                     /codon_start=1
                     /product="piercer of microtubule wall 2 protein"
                     /protein_id="NP_001185713.1"
                     /db_xref="CCDS:CCDS58363.1"
                     /db_xref="GeneID:145788"
                     /db_xref="HGNC:HGNC:44654"
                     /db_xref="MIM:619669"
                     /translation="MTDRNRDKKSTSPSNSDTEMKSEQLPPCVNPGNPVFSCMLDPKT
                     LQTATSLSKPQMIMYKTNSSHYGEFLPIPQFFPCNYTPKEQVFSSHIRATGFYQNNTL
                     NTAPDRTRTLDFPNIQHTL"
     exon            293..868
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
                     /inference="alignment:Splign:2.1.0"
     regulatory      761..766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
                     /note="hexamer: AATAAA"
     polyA_site      782
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
                     /note="major polyA site"
     regulatory      829..834
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
                     /note="hexamer: AATAAA"
     polyA_site      868
                     /gene="PIERCE2"
                     /gene_synonym="C14orf65; C15orf65"
ORIGIN      
        1 gatccccaca acatccggga gccggcgacc acttccagtt gctatggtta cgagttgcaa
       61 cctccagaaa gaattcgtgg tttcacccgg gaaaacagct ccccggatta aacggatagg
      121 tttacacata ctgatccacc cagctattca tcttctgttt gctgctttaa ttgggtgcgg
      181 ttaaaaggcc acgtccctag gcgttcaccg gctttcttgc catctgctgc atgaaaactg
      241 actttgccga aaaaattaac aaagaagagc gaaaatgaca gaccgcaacc gggataagaa
      301 aagtacttca ccttcaaatt cagacacaga aatgaaatct gaacaactgc ctccttgtgt
      361 gaaccctggc aatcctgtgt tttcatgtat gttggatcca aagacactcc agacagccac
      421 ctcactatca aaacctcaaa tgattatgta taaaaccaat tcaagtcatt atggtgaatt
      481 tctacctatt ccacagtttt tcccctgcaa ttatactcca aaggagcaag tattttcaag
      541 ccatatcaga gcaactggat tttatcaaaa taacactcta aatactgcac ctgacagaac
      601 cagaactctt gattttccta atattcaaca cactctatga aaatatattc ctttgtatat
      661 tgaagagaaa atatactcgg gaaaaatgag tgttaaatct aagggtagaa tacctaataa
      721 agaagataaa aagttttgaa tcaattttta aaataagtta aataaagtat ttcaactgat
      781 aactacatga cagtgacttt ttaaatgaaa atatgttcaa aatagcaaaa taaaaggtag
      841 aaagtgtttt agtccatgta atccaaaa
//
