LOCUS       NM_004671              11243 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens protein inhibitor of activated STAT 2 (PIAS2),
            transcript variant beta, mRNA.
ACCESSION   NM_004671 XM_938229
VERSION     NM_004671.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Rodrigues,J.S., Chenlo,M., Bravo,S.B., Perez-Romero,S.,
            Suarez-Farina,M., Sobrino,T., Sanz-Pamplona,R., Gonzalez-Prieto,R.,
            Blanco Freire,M.N., Nogueiras,R., Lopez,M., Fugazzola,L.,
            Cameselle-Teijeiro,J.M. and Alvarez,C.V.
  TITLE     dsRNAi-mediated silencing of PIAS2beta specifically kills
            anaplastic carcinomas by mitotic catastrophe
  JOURNAL   Nat Commun 15 (1), 3736 (2024)
   PUBMED   38744818
  REMARK    GeneRIF: dsRNAi-mediated silencing of PIAS2beta specifically kills
            anaplastic carcinomas by mitotic catastrophe.
            Erratum:[Nat Commun. 2024 Jul 11;15(1):5835. doi:
            10.1038/s41467-024-50135-0.. PMID: 38992026]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   Li,Y., Lin,H., Shu,S., Sun,Y., Lai,W., Chen,W., Hu,Z. and Peng,H.
  TITLE     Integrative transcriptome analysis reveals TEKT2 and PIAS2
            involvement in diabetic nephropathy
  JOURNAL   FASEB J 36 (11), e22592 (2022)
   PUBMED   36251411
  REMARK    GeneRIF: Integrative transcriptome analysis reveals TEKT2 and PIAS2
            involvement in diabetic nephropathy.
REFERENCE   3  (bases 1 to 11243)
  AUTHORS   Magalhaes,J., Tresse,E., Ejlerskov,P., Hu,E., Liu,Y., Marin,A.,
            Montalant,A., Satriano,L., Rundsten,C.F., Carlsen,E.M.M.,
            Rydbirk,R., Sharifi-Zarchi,A., Andersen,J.B., Aznar,S., Brudek,T.,
            Khodosevich,K., Prinz,M., Perrier,J.M., Sharma,M., Gasser,T. and
            Issazadeh-Navikas,S.
  TITLE     PIAS2-mediated blockade of IFN-beta signaling: a basis for sporadic
            Parkinson disease dementia
  JOURNAL   Mol Psychiatry 26 (10), 6083-6099 (2021)
   PUBMED   34234281
  REMARK    GeneRIF: PIAS2-mediated blockade of IFN-beta signaling: a basis for
            sporadic Parkinson disease dementia.
REFERENCE   4  (bases 1 to 11243)
  AUTHORS   Ghafouri-Fard,S., Hesami,O., Nazer,N., Sayad,A. and Taheri,M.
  TITLE     Expression of PIAS Genes in Migraine Patients
  JOURNAL   J Mol Neurosci 71 (10), 2053-2059 (2021)
   PUBMED   33763841
  REMARK    GeneRIF: Expression of PIAS Genes in Migraine Patients.
REFERENCE   5  (bases 1 to 11243)
  AUTHORS   Zheng,Y., Zhou,Z.M., Yin,L.L., Li,J.M. and Sha,J.H.
  TITLE     Molecular cloning and characterization of a novel splicing variant
            of PIASx
  JOURNAL   Acta Pharmacol Sin 25 (8), 1058-1064 (2004)
   PUBMED   15301740
REFERENCE   6  (bases 1 to 11243)
  AUTHORS   Kotaja,N., Aittomaki,S., Silvennoinen,O., Palvimo,J.J. and
            Janne,O.A.
  TITLE     ARIP3 (androgen receptor-interacting protein 3) and other PIAS
            (protein inhibitor of activated STAT) proteins differ in their
            ability to modulate steroid receptor-dependent transcriptional
            activation
  JOURNAL   Mol Endocrinol 14 (12), 1986-2000 (2000)
   PUBMED   11117529
REFERENCE   7  (bases 1 to 11243)
  AUTHORS   Minty,A., Dumont,X., Kaghad,M. and Caput,D.
  TITLE     Covalent modification of p73alpha by SUMO-1. Two-hybrid screening
            with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1
            interaction motif
  JOURNAL   J Biol Chem 275 (46), 36316-36323 (2000)
   PUBMED   10961991
REFERENCE   8  (bases 1 to 11243)
  AUTHORS   Moilanen,A.M., Karvonen,U., Poukka,H., Yan,W., Toppari,J.,
            Janne,O.A. and Palvimo,J.J.
  TITLE     A testis-specific androgen receptor coregulator that belongs to a
            novel family of nuclear proteins
  JOURNAL   J Biol Chem 274 (6), 3700-3704 (1999)
   PUBMED   9920921
REFERENCE   9  (bases 1 to 11243)
  AUTHORS   Liu,B., Liao,J., Rao,X., Kushner,S.A., Chung,C.D., Chang,D.D. and
            Shuai,K.
  TITLE     Inhibition of Stat1-mediated gene activation by PIAS1
  JOURNAL   Proc Natl Acad Sci U S A 95 (18), 10626-10631 (1998)
   PUBMED   9724754
REFERENCE   10 (bases 1 to 11243)
  AUTHORS   Wu,L., Wu,H., Ma,L., Sangiorgi,F., Wu,N., Bell,J.R., Lyons,G.E. and
            Maxson,R.
  TITLE     Miz1, a novel zinc finger transcription factor that interacts with
            Msx2 and enhances its affinity for DNA
  JOURNAL   Mech Dev 65 (1-2), 3-17 (1997)
   PUBMED   9256341
  REMARK    Erratum:[Mech Dev 1997 Dec;69(1-2):219]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG718291.1, AF077954.1,
            AC090241.9 and AF086240.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004671.4.
            
            Summary: This gene encodes a member of the protein inhibitor of
            activated STAT family, which function as SUMO E3 ligases and play
            important roles in many cellular processes by mediating the
            sumoylation of target proteins. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene. Isoforms of the encoded protein enhance the sumoylation of
            specific target proteins including the p53 tumor suppressor
            protein, c-Jun, and the androgen receptor. A pseudogene of this
            gene is located on the short arm of chromosome 4. The symbol MIZ1
            has also been associated with ZBTB17 which is a different gene
            located on chromosome 1. [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (beta) encodes isoform (beta).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.190155.1,
                                           SRR1660809.50898.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000585916.6/ ENSP00000465676.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               BG718291.1         33-218
            187-2009            AF077954.1         25-1847
            2010-10566          AC090241.9         92840-101396
            10567-11237         AF086240.1         1-671
            11238-11243         AC090241.9         102068-102073
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.1"
     gene            1..11243
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="protein inhibitor of activated STAT 2"
                     /db_xref="GeneID:9063"
                     /db_xref="HGNC:HGNC:17311"
                     /db_xref="MIM:603567"
     exon            1..186
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..2028
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /EC_number="2.3.2.27"
                     /note="isoform beta is encoded by transcript variant beta;
                     zinc finger, MIZ-type containing 4; E3 SUMO-protein ligase
                     PIAS2; DAB2-interacting protein; msx-interacting zinc
                     finger protein; androgen receptor-interacting protein 3;
                     protein inhibitor of activated STAT X; E3 SUMO-protein
                     transferase PIAS2"
                     /codon_start=1
                     /product="E3 SUMO-protein ligase PIAS2 isoform beta"
                     /protein_id="NP_004662.2"
                     /db_xref="CCDS:CCDS32824.1"
                     /db_xref="GeneID:9063"
                     /db_xref="HGNC:HGNC:17311"
                     /db_xref="MIM:603567"
                     /translation="MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHL
                     LKSGCSPAVQIKIRELYRRRYPRTLEGLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIH
                     SLPSTSVTPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVL
                     IKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAET
                     SCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQ
                     ISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSE
                     IATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKA
                     AYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVSSQPCTKIESSSVL
                     SKPCSVTVASEASKKKVDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQ
                     PSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPISSMSSDLPGLDFLSLIPVDPQYCPP
                     MFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD"
     exon            187..661
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            662..746
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            747..797
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            798..888
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1023
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1129
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1203
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1364
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1498
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1670
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1810
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1848
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..11243
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2822..2827
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="hexamer: TATAAA"
     polyA_site      2855
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="major polyA site"
     regulatory      3993..3998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="hexamer: AATAAA"
     polyA_site      4019
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="major polyA site"
     regulatory      4374..4379
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="hexamer: AATACA"
     polyA_site      4402
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
     regulatory      5504..5509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="hexamer: AATAAA"
     polyA_site      5524
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
     regulatory      6041..6046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="hexamer: AATAAA"
     polyA_site      6071
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
     regulatory      6425..6430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="hexamer: ATTATA"
     polyA_site      6446
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
     regulatory      11219..11224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
                     /note="hexamer: ATTAAA"
     polyA_site      11243
                     /gene="PIAS2"
                     /gene_synonym="ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4"
ORIGIN      
        1 acgtgacggg cccggctgta gcggcggcgg ccgcggcgtc ttaagcggcg cccagtgcag
       61 gatggtgctg gaggcggcgg cggccgtggt ggcggcagcg tcgttggcgg cagcgggagt
      121 gggtgcggcg gcagcggcgg cggcgcccgc gggtggtata aaatggcgga tttcgaagag
      181 ttgaggaata tggtttctag ttttagggtt tctgaactac aagtattact aggctttgct
      241 ggacggaata aaagtggacg caagcatgac ctcctgatga gggcgctgca tttattgaag
      301 agcggctgca gccctgcggt tcagattaaa atccgagaat tgtatagacg ccgatatcca
      361 cgaactcttg aaggactttc tgatttatcc acaatcaaat catcggtttt cagtttggat
      421 ggtggctcat cacctgtaga acctgacttg gccgtggctg gaatccactc gttgccttcc
      481 acttcagtta cacctcactc accatcctct cctgttggtt ctgtgctgct tcaagatact
      541 aagcccacat ttgagatgca gcagccatct cccccaattc ctcctgtcca tcctgatgtg
      601 cagttaaaaa atctgccctt ttatgatgtc cttgatgttc tcatcaagcc cacgagttta
      661 gttcaaagca gtattcagcg atttcaagag aagtttttta tttttgcttt gacacctcaa
      721 caagttagag agatatgcat atccagggat tttttgccag gtggtaggag agattataca
      781 gtccaagttc agttgagact ttgcctggca gagacaagtt gccctcaaga agataactat
      841 ccaaatagtc tatgtataaa agtaaatggg aagctatttc ctttgcctgg ctatgcacca
      901 ccgcctaaaa atgggattga acagaagcgc cctggacgcc ccttgaatat tacatcttta
      961 gttaggttat cttcagctgt gccaaaccaa atttccattt cttgggcatc agaaattggg
     1021 aagaattact ctatgtctgt atatcttgta cggcagctta catcagccat gttattacag
     1081 agattaaaaa tgaaaggtat tagaaaccct gatcattcca gagcactaat taaagaaaaa
     1141 cttactgcag atcctgatag tgaaattgct acaactagcc ttcgggtatc cttgatgtgc
     1201 cctttaggaa aaatgaggct gacaatccca tgccgtgcag tgacttgtac acatctgcag
     1261 tgttttgatg ctgccctcta tctacaaatg aatgagaaaa agcccacctg gatttgtcct
     1321 gtgtgtgaca aaaaagctgc ctatgaaagt ctaatattag atgggctttt tatggaaatt
     1381 ctcaatgact gttctgatgt agatgagatc aaattccaag aagatggttc ttggtgtcca
     1441 atgagaccga agaaagaagc tatgaaagta tccagccaac cgtgtacaaa aatagaaagt
     1501 tcaagcgtcc tcagtaagcc ttgttcagtg actgtagcca gtgaggcaag caagaagaaa
     1561 gtagatgtta ttgatcttac aatagaaagc tcttctgacg aagaggaaga ccctcctgcc
     1621 aaaaggaaat gcatctttat gtcagaaaca caaagcagcc caaccaaagg ggttctcatg
     1681 tatcagccat cttctgtaag ggtgcccagt gtgacttcgg ttgatcctgc tgctattccg
     1741 ccttcattaa cagactactc agtaccattc caccatacgc caatatcaag catgtcatca
     1801 gatttgccag gtttggattt tctttccctt attccagttg atccccagta ctgtcctcct
     1861 atgtttttgg atagtctcac ctcaccctta acagcaagca gtacgtctgt caccaccacc
     1921 agctcccatg aaagcagtac tcatgttagt tcatccagca gcaggagtga gacaggggtc
     1981 ataaccagca gtggaagtaa cattcctgac atcatctcat tggactaaag gaggactcac
     2041 ttgattctgg gaatcattca tcagaactgc tttttcttgg atctctagtc tgtggaaacg
     2101 ttcttttttt ttttttttta ataatttggt atttattgaa agtcagatgg attcttttgc
     2161 tttctgaggg gtgaacacag aagactgcaa caagaaccaa atgacatgca aggatttttc
     2221 ttaattatgc tgtactctca gcatcagata cattcaacca taactgtatc ttcctgagag
     2281 atggatttca ctttcatacg ttaatggatg gagtctacaa atcagtgaaa aaagtttttg
     2341 ttaaaataaa gagcaataaa attatgtaaa tattcaagta accttttttc taattaaaaa
     2401 aaaaattgta atcactgatt ctagaatgac agcaacttct gtttaacctt acgtgaagga
     2461 aaaaaaatat atggaaagaa cttaatgttt gctgaatgag tcctttccat ggagatttgc
     2521 tctgtttcat tgaagtaatg aagtttcagt acgtggccaa agcttggatt ccacttttcg
     2581 tcctgcattt ccacttttct tcgtgattgc atcaaagaaa aatggcattc gtgttacttg
     2641 gtttttttcc tgcttatcat ttacactttg acgttgtttg ctaatgagtg ctgtgtgaag
     2701 ttctgggatt tggctactct tcattggtga ttatgtttaa aagcactatg cagattctgc
     2761 cctgacataa agtgtgttat tatgttttta gtaattgtac atttttcatt ctagagtttt
     2821 ctataaattt gaggcttgcc ttctcaaaaa agaaactatg cagccattga atgaaatgtc
     2881 tttggggtac ggtgtgactg gaatgtttgt tagaaatttg ttcacactat caaatattga
     2941 tatcttggag ccagcagaag agcagatttt gggaggtggt aataacaaaa tttaatttct
     3001 tcccaacaac ttaattttct catttatttt acagaatagt agtgaaatat ttgatgaaac
     3061 tttgtatttt ggtagcacta catagaaaat gtgttttaga tttatgatga tcatatttct
     3121 caccaatgta atttcagtct cagcagtgat tttcaaactt agggaaaggg acagcattag
     3181 attttttttt tttttcattt ttttaaaatg atatcttacc tgaaactaca aacgacaaaa
     3241 gagaattaga aatgtttgaa ttaaagtgaa gaagggttgg gggagatggg cctgaaccca
     3301 cttcctgtct caatccatgc taccccaaac actccaggga acctctgagg ttttattggg
     3361 tgcactttga aaatttctct tctatagtgt gtttgtttga ttttaaatca cagagaaaac
     3421 tgggttttac tcttagagaa acattttcat ccagtttttt agtttgcttc atttgacttc
     3481 ctaaatcatt tttgagttca caaggatttg gtacttttct gtttagcttt ctctctctaa
     3541 gctttatcta ccttaaaaac aaagtccttt ttttaatggc cagtccaacc aattgatttc
     3601 tcaaactgaa gtgcccaggt gtggactcat caatttccgt tagaataggg acatcctact
     3661 taagagttgg tgcagctcca aggagctgac ttgtccttgc ttggggtttt tttttttttt
     3721 ctttcacctt ctcaagtttc catggccttt gtgtgttctt tttatgttga tttaaattca
     3781 tatggttttc cacaaatccc ttctttggct acattgtctc cttattcaat ggattatccc
     3841 tttgtggggg ctgcttattt taaagatgtt gggggggaaa caaacccaaa tctacgagca
     3901 gtagttgcac atagttgcca gttttacctt cttagtcatt agatttccaa accatgttgc
     3961 agttttttgg tccagatata gtatttcttt ctaataaagt tttatgttgc tgctctaaat
     4021 acagatgcaa tatttattga ctctgtaatc agatagaaaa aacttaactt ggtttgtgtg
     4081 gtatgactta taaagaaatg atgtatattt gttattttgt taccctttag attgtcagag
     4141 actcccccaa tttaatcaac aaagttttat aaagtaaatg aaaatattaa tagaaattag
     4201 tttatttact tggttcttat aactgatatc tctgtgcttt tataattgtg atttgttttt
     4261 ttgttttttt ttctattttc tgtgaacagt tttaatgttc ggttttggtg ttttacactg
     4321 aaattacata taaattttta atttatttca tacaggcaac ttgcatttta aaaaatacac
     4381 tttgaagttt atcatcttga aattggggct tacgttgttt atctgtcttg agcattagta
     4441 ctttatgact ttggccttat ggcaacatca tgattattaa tccgtcagcc tttaatgtgg
     4501 tcactgtttc ttatccagac cctgactttc tagtagttta ttttgctagc ccagatttct
     4561 gcttaaccaa atataaggga gtttcagagg ggtgatctta gctgtcacct agattctgtc
     4621 agccaaccag tgattgtctg gaatatctta ggaaatgaac tgtaattgtc agcctcctaa
     4681 aatcaatttt tttttttttt tttttgaggc tgagtcttgc tcttgttgcc cgggatggag
     4741 tgcaatggtg cgatcttggc tcaccgcaac ctccacctac agggttcaag cgattctcct
     4801 gcctcagcct cccgagtagc tgggattaca ggcatgtatc accacgccca gctaattttg
     4861 tatttttagt agatacgggg tttctccatt ttggtcaggc tggtctcgaa ctcccgacct
     4921 caggtgatcc gcctgccttg gcctcccaaa gtgctgggat ttacaggcgt gagccaccgc
     4981 gcctggccaa aataaaatgt ttttatcttt cttcacttag aactatgaag gccttttctt
     5041 gttctggtaa ccccccacag tgtaattggt gctgaaaata gtttcgtgcc tgaggtctgt
     5101 tgtcactcac tcactttctg acaattaggt gagccacttg aggattttct ggcctgaagt
     5161 tataaaaact tggcatctgt cctaaggtaa ttgtaaaggc aaaatgaaag cattgaggtg
     5221 aatttaggtt atatggcatt tatctggtaa tgtgtttcag aaaagctaga atgaaaatgt
     5281 gatgtaaggg atagcaagtg taggttgatt cataattctt ggtataaaac caagttttcc
     5341 ttagtcttga ggaatttgaa gaaaggtaaa atgttggatt ttaagcaaag taggattgac
     5401 cagagactgt tctgactttg acattcagac ctttcagatg ttcttccttc agaccagtcc
     5461 aagacactca gcatttccag ccaagttgtc ttttttttct gccaataaag tattcattag
     5521 ttgacgtttg tttgtatttt aagtaaagtc aatttttttt aaagcattaa tttaattcac
     5581 gggtatgcct ttcatttctc tggccttcag ttggtttaaa aaaaaaaaaa aaaaaaaaag
     5641 cacttagccg gaccattctt tctttagtaa acatttttat tttcttaatt tttcctgaaa
     5701 gattttcttc attttcatgg aatgtcataa tgacactacc attattttct agctctttgc
     5761 ataatagaaa aatgtagagc agtgcaccat ttaaacacgt ctgacattgg atttagtggt
     5821 cctaggttca tgtgatttgg gagccttaag ttgttattta gattgattca gcctgattca
     5881 ccatcttttt tactacagtt ctacatgaat gtggtgaaac aggtagaaga aaattaagca
     5941 tttctctgct tttggagtct aatgttcctg cctagtgact ggtttgtaac acccccctca
     6001 cccctacttt gttaggaacc tgcaattcct aaaagaaata aataaagctg tacccaatgt
     6061 ccattcttta atgcttgcct ggttgaataa tgtttgtttt ttctgtttat gtcagtagta
     6121 catgcttatt gtggaaaatt tagacaatac agaaaagtat aacaattaat tgcctatgat
     6181 tctgtcacca gaaaaaatca cttgatattt ttgcatcttc cttgccattg tctttgctac
     6241 ttctgcatat ttttacttag tttcatcatt ctcagtgtaa tttatatctt ggggtttttc
     6301 cccgcaacct acaggcatta ttccatgtca ttaaaactgt gtcaactatt attaatttta
     6361 tggtactcca ttgttatgga tgtgccatgt ttcatataac ctttacttat tttgaacagt
     6421 ttagattata tctcataatg ccacaatgaa tgtctttgtg tataaattat tttcttagga
     6481 taaaatactg gaggtggaat ttctagtcaa gggtatatga acacttttgt agttcttgat
     6541 tcataccact tttccacatg ggtatgttaa tttatactgc agctagatgg tatgggaagg
     6601 gtggtctagc catattttcc aagaacatgc aacgttttat ctttatgcag gcatctgcac
     6661 taagaccctc tgcctttctg actctatgga acggggttaa ctgatatgga gatcattggc
     6721 agagattttc ccaagctttc ttagttttaa atatatctga catgggccgg gtatggaggc
     6781 tcacgcctgt aatcccagca ctttgggagg ccaaggcggg cagataatct gaagtcagga
     6841 gttggagacc agcctggcta acatggaaca tggtgaaacc ccatctctag caaaaacata
     6901 aattaggtgg gtgtgatggc aggcgcctgt agtcccagct acttgggaga ccgaggcagg
     6961 agaatcgctt gaacccagga ggcggaggat ttgcagtgat ccgagattgt gccactgcac
     7021 tccagcctgg gtaacaaagc aagactctct aaaaaaaaaa aaaaaaaaaa aaaaaaatat
     7081 atatatatat atatatatat atataaaatc tgacatgttt cagaaacacc tacgtatggc
     7141 tatccaagct tttcccatga ttttactggc actgtaaagt tgcacaaata ttctttgtca
     7201 gactacgttt tccatcttta gttcaaaagt tttgtcactt tttaaaaaaa gcctcttgta
     7261 cctaaggcct gcccttaaga ggtcagctct ctttttcctc ttcctgtagg ccccattatg
     7321 gcctttctga gctccccaaa gccagtagaa tctactgagc caaaggcatt ccatccccaa
     7381 actgaaaagt ggtaaggcta aaagctataa aaacaggcaa acattgcctg ttttcacttt
     7441 atattccctt atgtttccaa taagggtagc tgcagggaaa gaaaaaaaca gattgctacc
     7501 ttcagatgtt tgaggtacaa cattttcttg attggcgtag cacaatcatt tcccaaactg
     7561 cttcctattt ctgaatggct tttttaaaaa gctttatttt gtgttctcaa agcttaatta
     7621 ttttaagtga cgaagagtgt taccgtgttg tagaatagca tatgcctctt attttggcct
     7681 tcatcaaact gtttaataaa tgtttagagt gttttgtaag tacacgtgga atagagtgtt
     7741 acagaagttt tggttccaga catcaggcag cttataatca aggttccaaa aatcaagaat
     7801 tcttaagctg ggcatggtgg ctcatgcctg taatcccagc actttgggaa gccaaggcag
     7861 gcaaatcact tgaggccagg agtttgagac cagcctggcc aacttggtaa aaccccatct
     7921 ctactaaaaa tacaaaaaat tagggcatga tggctcatgt ctgtaatccc agctacttgg
     7981 gaggctgagg gatgagaatt gcttgaacct gagaggcgga ggttgcagtg actcaggatc
     8041 atgaccctgt actccagcct gggtgacaga gtgagaccaa aaaaaaaaaa aaaaaaaaaa
     8101 aaaaaaagta acaggcattg tgcaaagaat tttccaagaa tgtctcattc ttgcaatggc
     8161 ttctcagagg aaagtggtgt tattccccat tttacaaatg aaacaggttt aggtaagttt
     8221 cccaaggtca cagtgaatga gtagtggact tgggagtctg acctagactt accccaaaag
     8281 ctctctgcgt aaactgaagt gacgttaatt agctgtgcta tctaggagac tgatatggga
     8341 atctagactt aaaattatga gaactggttt cagatgatgg ccagagtaat aaggaaggag
     8401 tggatgctga agccatttca aaggaagact caagattcag tgactgaata taggagtaag
     8461 ggttgagtgc atttttcatc catttttcaa gcctgggtaa cttggatgtt aatggcacta
     8521 tctacagaga caggaggcag tttagagtga agacgaatca tgtatgggag acagtgatga
     8581 atgttaagca tgggatttca gaaatgaagc atccatgagg ctcattcctg gtttttctgc
     8641 agtcttttaa ataaaagtac aaacctccct tgtcatccat ttttgccttc acttatctcc
     8701 attctgtatt atgttctagt cacgccccag tcactgatcc cttcctaggc catacttggc
     8761 tacatcttag ccttttcttt tgatttttct cttcactgcc ttgcccatta caaatttggc
     8821 tgccacctga ggccctgcca taccctacag ctcacaccag cgtgggctgt gttcacattc
     8881 tacatatctc aagatacagt ggttgctgtc cttcccccct actgcatcgt tacacgtctc
     8941 taaaaaatct ctgcctctga agctcatgcc aagaggttat actatcagct acctctagtc
     9001 ttataactgc ctgatcattc cctttgattt gctggaagct ttgactcaca gtgttccttg
     9061 ccttccttga ttctggtcat tattattaat atcttgaaca ttctatagat gatttatcca
     9121 agttggtctg tgaggtattg gttactttac ctccagcatt ttcgtctact gaaccacgct
     9181 ttctgttttc ctctttgcta cttgtccaga ctgttctttc attattgcag ccttgaagaa
     9241 atcctcagta acctatccat cctccgacat gacgtagcat agttcctttg cccattctca
     9301 tcagttataa caaatgttat tgccctcttt tcaggccact tccttctcag aagatgactt
     9361 tgctgcttaa ttcaaacaga agggacagtc cgaaagcatg atttcttccc cacctatgac
     9421 cacctccatc cttttttgtt tccagtttca gagaatgagg taggccttta aggccaactg
     9481 actctggtct cctcctgtca tcttcaggac cttcctctgt cagacttccc ctctcttatc
     9541 tccatgtctc tcacctctct actgctgctt ttcttggctt acaaatggtc tcagtcatcc
     9601 agtatttgag attatattag gtgccaggca ttgtatcagg cacttggaac acaaggcagg
     9661 atcctgcccc caccaaagtt gtgctccaat agcgtcaaca gaggtgtgaa atagggagat
     9721 tacaatgtta atggtaaaag acaaagtata gggtgctaca agagcatgta aatgaggcac
     9781 ctcaatgatt taatagaagt aatgtggaga aatgtgagga ttgatgaaaa gcttgtccaa
     9841 aaagggccag aaatgtttag aattgctgga agtttgctaa ggaatattga gaaatatgca
     9901 tagatagatc gtgaagggcc ttgtaaaaca gtgatgagac attgaagagt tttcatgatc
     9961 attagtagta atgagaattt gttgagtttg gcatgattat ctccatatta caaatgtgga
    10021 aacagattct gacaggataa gcgacttact tgcccagggt catgcagctt gtaattgagg
    10081 agtcaggttt caaatccagt tttaactact ttctcagagg cctttcttga tcattctatc
    10141 taaaaagcaa gtgttcccta actattatcc cagtaccctg gtttatatac tcttaataca
    10201 taaaataatc cctgcatatt gtgtgtttcc cctactagat tgtaagctcc atgagggcag
    10261 aggtgataac ttttttcact tgcatcccag agcctaacat gatatgggct acatagaagg
    10321 tgcccagtaa atattggtgg atgaagaaat ggtggataac catttgcatt tgacttatgc
    10381 tgactggaaa ttcaatacct ttggaatata taatacctca gcagtaagca ttcaccaaga
    10441 gaggaaaaca gaaggaggtg catgtttagg gtaggtggta cgggagatgg atgctgaggt
    10501 gttgaaaggc agcatactca ccttctgaac tccaaaactt acaggggcaa gggccattaa
    10561 tgaggggtgg gtagagcagt gcacagtggg gataatatgg gagtaggtgt attaaacctg
    10621 gaagtgtaag caactccttg tagacccacc ctactgtgta taaaaggctg gagaaattct
    10681 tcatatgatc tactctaggt tatgtactga ggaccacctt atctgggtca ggtacactac
    10741 acagagtgct acaatataat tgtaaacata atttatcctc actgtgatgg ctagccacct
    10801 gacccaaatc attattagac tcaatacttt ggaactggaa caaaaatcag ttgacctgat
    10861 gtctgaaaga catgcccacc tgctatttga caagctatag acatatccta ccaattgatg
    10921 ggtagtagca ctgaaactat gaaagtggat gaactcatcc agaggagttg atgagtgaga
    10981 aaagaagact ggaaaaggaa cacaaggaaa tcaagaagca gcagctgaga ctgaaggaaa
    11041 ataacgtggt attacagagg ccaaaaaata gagtatttca agaagcaagt ggttagtgga
    11101 gttaaaagct gctgatacaa cctaaaaaag taagtatcca ttttttaaaa aactttcatt
    11161 taaaatacca ccatatttta ttgaatctaa gtacctgatt gtaagacatt aaagaaaaat
    11221 taaacctgct aattaaacta tga
//
