LOCUS       NM_016166               7980 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens protein inhibitor of activated STAT 1 (PIAS1),
            transcript variant 2, mRNA.
ACCESSION   NM_016166
VERSION     NM_016166.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Zhang,X.Y., Li,S.S., Gu,Y.R., Xiao,L.X., Ma,X.Y., Chen,X.R.,
            Wang,J.L., Liao,C.H., Lin,B.L., Huang,Y.H. and Lian,Y.F.
  TITLE     CircPIAS1 promotes hepatocellular carcinoma progression by
            inhibiting ferroptosis via the miR-455-3p/NUPR1/FTH1 axis
  JOURNAL   Mol Cancer 23 (1), 113 (2024)
   PUBMED   38802795
  REMARK    GeneRIF: CircPIAS1 promotes hepatocellular carcinoma progression by
            inhibiting ferroptosis via the miR-455-3p/NUPR1/FTH1 axis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Ho,P.J., Kweon,J., Blumensaadt,L.A., Neely,A.E., Kalika,E.,
            Leon,D.B., Oh,S., Stringer,C.W.P., Lloyd,S.M., Ren,Z. and Bao,X.
  TITLE     Multi-omics integration identifies cell-state-specific repression
            by PBRM1-PIAS1 cooperation
  JOURNAL   Cell Genom 4 (1), 100471 (2024)
   PUBMED   38190100
  REMARK    GeneRIF: Multi-omics integration identifies cell-state-specific
            repression by PBRM1-PIAS1 cooperation.
REFERENCE   3  (bases 1 to 7980)
  AUTHORS   Wang,L., Zeng,W., Wang,C., Lu,Y., Xiong,X., Chen,S., Huang,Q.,
            Yan,F. and Huang,Q.
  TITLE     SUMOylation and coupling of eNOS mediated by PIAS1 contribute to
            maintenance of vascular homeostasis
  JOURNAL   FASEB J 38 (1), e23362 (2024)
   PUBMED   38102979
  REMARK    GeneRIF: SUMOylation and coupling of eNOS mediated by PIAS1
            contribute to maintenance of vascular homeostasis.
REFERENCE   4  (bases 1 to 7980)
  AUTHORS   Wu,C., Zhu,X., Dai,Q., Chu,Z., Yang,S. and Dong,Z.
  TITLE     SUMOylation of SMAD4 by PIAS1 in Conjunction with Vimentin
            Upregulation Promotes Migration Potential in Non-Small Cell Lung
            Cancer
  JOURNAL   Front Biosci (Landmark Ed) 28 (8), 192 (2023)
   PUBMED   37664931
  REMARK    GeneRIF: SUMOylation of SMAD4 by PIAS1 in Conjunction with Vimentin
            Upregulation Promotes Migration Potential in Non-Small Cell Lung
            Cancer.
REFERENCE   5  (bases 1 to 7980)
  AUTHORS   Belaguli,N.S., Zhang,M., Garcia,A.H. and Berger,D.H.
  TITLE     PIAS1 is a GATA4 SUMO ligase that regulates GATA4-dependent
            intestinal promoters independent of SUMO ligase activity and GATA4
            sumoylation
  JOURNAL   PLoS One 7 (4), e35717 (2012)
   PUBMED   22539995
  REMARK    GeneRIF: PIAS1 is a SUMO ligase for GATA4 that differentially
            regulates GATA4 transcriptional activity independent of SUMO ligase
            activity and GATA4 sumoylation.
REFERENCE   6  (bases 1 to 7980)
  AUTHORS   Kotaja,N., Aittomaki,S., Silvennoinen,O., Palvimo,J.J. and
            Janne,O.A.
  TITLE     ARIP3 (androgen receptor-interacting protein 3) and other PIAS
            (protein inhibitor of activated STAT) proteins differ in their
            ability to modulate steroid receptor-dependent transcriptional
            activation
  JOURNAL   Mol Endocrinol 14 (12), 1986-2000 (2000)
   PUBMED   11117529
REFERENCE   7  (bases 1 to 7980)
  AUTHORS   Liao,J., Fu,Y. and Shuai,K.
  TITLE     Distinct roles of the NH2- and COOH-terminal domains of the protein
            inhibitor of activated signal transducer and activator of
            transcription (STAT) 1 (PIAS1) in cytokine-induced PIAS1-Stat1
            interaction
  JOURNAL   Proc Natl Acad Sci U S A 97 (10), 5267-5272 (2000)
   PUBMED   10805787
REFERENCE   8  (bases 1 to 7980)
  AUTHORS   Tan,J., Hall,S.H., Hamil,K.G., Grossman,G., Petrusz,P., Liao,J.,
            Shuai,K. and French,F.S.
  TITLE     Protein inhibitor of activated STAT-1 (signal transducer and
            activator of transcription-1) is a nuclear receptor coregulator
            expressed in human testis
  JOURNAL   Mol Endocrinol 14 (1), 14-26 (2000)
   PUBMED   10628744
REFERENCE   9  (bases 1 to 7980)
  AUTHORS   Liu,B., Liao,J., Rao,X., Kushner,S.A., Chung,C.D., Chang,D.D. and
            Shuai,K.
  TITLE     Inhibition of Stat1-mediated gene activation by PIAS1
  JOURNAL   Proc Natl Acad Sci U S A 95 (18), 10626-10631 (1998)
   PUBMED   9724754
REFERENCE   10 (bases 1 to 7980)
  AUTHORS   Valdez,B.C., Henning,D., Perlaky,L., Busch,R.K. and Busch,H.
  TITLE     Cloning and characterization of Gu/RH-II binding protein
  JOURNAL   Biochem Biophys Res Commun 234 (2), 335-340 (1997)
   PUBMED   9177271
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF167160.1, AC107871.10 and
            AA837658.1.
            
            On Feb 27, 2020 this sequence version replaced NM_016166.2.
            
            Summary: This gene encodes a member of the protein inhibitor of
            activated STAT (PIAS) family. PIAS proteins function as SUMO E3
            ligases and play important roles in many cellular processes by
            mediating the sumoylation of target proteins. This protein plays a
            central role as a transcriptional coregulator of numerous cellular
            pathways includign the STAT1 and nuclear factor kappaB pathways.
            Alternate splicing results in multiple transcript variants.
            [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (2) uses an alternate 5' structure
            which results in a distinct 5' UTR and 5' coding region compared to
            variant 1. The encoded isoform (2) is shorter and has a distinct
            N-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.10216.1,
                                           SRR1803616.31761.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000249636.11/ ENSP00000249636.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2208              AF167160.1         85-2292
            2209-5409           AC107871.10        76715-79915
            5410-5597           AA837658.1         29-216              c
            5598-7980           AC107871.10        80104-82486
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q23"
     gene            1..7980
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="protein inhibitor of activated STAT 1"
                     /db_xref="GeneID:8554"
                     /db_xref="HGNC:HGNC:2752"
                     /db_xref="MIM:603566"
     exon            1..36
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..1968
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2; AR
                     interacting protein; zinc finger, MIZ-type containing 3;
                     DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; E3
                     SUMO-protein ligase PIAS1; gu-binding protein; RNA
                     helicase II-binding protein; DEAD/H box-binding protein 1;
                     protein inhibitor of activated STAT protein 1; E3
                     SUMO-protein transferase PIAS1"
                     /codon_start=1
                     /product="E3 SUMO-protein ligase PIAS1 isoform 2"
                     /protein_id="NP_057250.1"
                     /db_xref="CCDS:CCDS45290.1"
                     /db_xref="GeneID:8554"
                     /db_xref="HGNC:HGNC:2752"
                     /db_xref="MIM:603566"
                     /translation="MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHL
                     LKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGH
                     PASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDN
                     SQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPN
                     LCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGR
                     NYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLL
                     CPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLF
                     MEILKYCTDCDEIQFKEDGTWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQS
                     SNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRT
                     PSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAA
                     AVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRES
                     HSHTVTNRSSTDTASIFGIIPDIISLD"
     exon            37..481
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            482..566
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            567..614
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            615..705
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            706..840
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            841..946
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1020
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1181
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1312
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1493
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1636
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1674
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..7980
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2112..2117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="hexamer: AAGAAA"
     polyA_site      2133
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="major polyA site"
     regulatory      5574..5579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="hexamer: AATAAA"
     regulatory      5579..5584
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="hexamer: AAGAAA"
     polyA_site      5592
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="major polyA site"
     polyA_site      5597
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="major polyA site"
     regulatory      7960..7965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
                     /note="hexamer: ATTAAA"
     polyA_site      7980
                     /gene="PIAS1"
                     /gene_synonym="DDXBP1; GBP; GU/RH-II; ZMIZ3"
ORIGIN      
        1 gacagacgca agatggcgga cagtgcggaa ctaaagcaaa tggttatgag ccttagagtt
       61 tctgaactcc aagtactgtt gggctacgcc gggagaaaca agcacggacg caaacacgaa
      121 cttctcacaa aagccctgca tttgctaaag gctggctgta gtcctgctgt gcaaatgaaa
      181 attaaggaac tctataggcg gcggttccca cagaaaatca tgacgcctgc agacttgtcc
      241 atccccaacg tacattcaag tcctatgcca gcaactttgt ctccatctac cattccacaa
      301 ctcacttacg atggtcaccc tgcatcatcg ccattactcc ctgtttctct tctgggacct
      361 aaacatgaac tggaactccc acatcttaca tcagctcttc acccagtcca tccggatata
      421 aaacttcaaa aattaccatt ttatgattta ctggatgaac tgataaaacc caccagtcta
      481 gcatcagaca acagtcagcg ctttcgagaa acctgttttg catttgcctt gacaccacaa
      541 caagtgcagc aaatcagtag ttccatggat atttctggga ccaaatgtga cttcacagta
      601 caggtccagt taaggttttg tttatcagaa accagttgtc cacaagaaga tcacttccca
      661 cccaatcttt gtgtgaaagt gaatacaaaa ccttgcagcc ttccaggtta ccttccacct
      721 acaaaaaatg gcgtggaacc aaagcgaccc agccgaccaa ttaatatcac ctcacttgtc
      781 cgactgtcca caacagtacc aaacacgatt gttgtttctt ggactgcaga aattggaaga
      841 aactattcca tggcagtata tcttgtaaaa cagttgtcct caacagttct tcttcagagg
      901 ttacgagcaa agggaataag gaatccggat cattctagag ctttaattaa agagaagttg
      961 actgcggatc cagacagtga aatagctaca accagcctaa gggtttctct actatgtcca
     1021 cttggtaaaa tgcggctgac aattccgtgt cgggccctta catgttctca tctacaatgt
     1081 tttgacgcaa ctctttacat tcagatgaat gagaaaaaac caacctgggt ttgtcctgtc
     1141 tgtgataaga aggctccata tgaacacctt attattgatg gcttgtttat ggaaatccta
     1201 aagtactgta cagactgtga tgaaatacaa tttaaggagg atggcacttg ggcaccgatg
     1261 agatcaaaaa aggaagtaca ggaagtttct gcctcttaca atggagtcga tggatgcttg
     1321 agctccacat tggagcatca ggtagcgtct caccaccagt cctcaaataa aaacaagaaa
     1381 gtagaagtga ttgacctaac catagacagt tcatctgatg aagaggaaga agagccatct
     1441 gccaagagga cctgtccttc cctatctccc acatcaccac taaataataa aggcatttta
     1501 agtcttccac atcaagcatc tccagtatcc cgcaccccaa gccttcctgc tgtagacaca
     1561 agctacatta atacctccct catccaagac tataggcatc ctttccacat gacacccatg
     1621 ccttacgact tacaaggatt agatttcttt cctttcttat caggagacaa tcagcattac
     1681 aacacctcct tgcttgccgc tgcagcagca gcagtttcag atgatcaaga cctcctacac
     1741 tcgtctcggt ttttcccgta tacctcctca cagatgtttc ttgatcagtt aagtgcagga
     1801 ggcagtactt ctctgccaac caccaatgga agcagtagtg gcagtaacag cagcctggtt
     1861 tcttccaaca gcctaaggga aagccatagc cacaccgtca caaacaggag cagcacggac
     1921 acggcatcca tctttggcat cataccagac attatttcat tggactgatt cccaggccct
     1981 gctgctccca tccccacccc agatcgaatg aacttggcag aaagaagaga actttgtgct
     2041 ctgttttacc ttactctgtt tagaaaagta tacaagcgtg ttttttttcc tttttttagg
     2101 gaaaaaatta aaagaaatgt acagagaaca aaactatatt ttcagtttta cttttgtata
     2161 taaatctaag actgcctgtg tgataaaaca cttgtttaaa aaaaaaaagg aaagaaaaga
     2221 aaaaagaaaa acaagcaccc acaaaccacc ttcagttcat ttttttctgg attctgaaga
     2281 tttttcatta tttgtcctat ggttttggtt ttatttgact tcgatggcat tattttattt
     2341 gcaataacag aaaaggaatt gcatgtatga agttttcaat cgtgggcttt tctttgttgt
     2401 ggggaggggg tcgggggata gtttgatttc cattttctga aaacgacaga cttggattct
     2461 gtttgtgtgt gcatatttta tccagcctta agttataaag ctcatctgtc ccgctgcatt
     2521 ccctgtgtat tttcaggaca tggctcgtgg gtgtgtgtgt tcattgtgtg cgtctgtatg
     2581 tatttttctg tcatcactgt tccctctcct cccgagtgtg cattcagtta atataatcag
     2641 ttgcttgctt ctttcaaagt gctttgaagg tcttgaactc acgtgtgagc atctttatca
     2701 actatcccaa ttgcatgttc tccatcacat attctcttat ttgctctgta ccccctgaga
     2761 atatgtttta gagatattgg aataaagctg tctgggtaag gagtaggctt agccgaccta
     2821 tgaataatac actttagtct agttctttat tctaaatctg gattgccagt attgtgtatt
     2881 taaaccaagt ctgtgaatac ctgctttttt tggccacaga gtaacaagtt ttcatgtaag
     2941 atcttcatac caaagtagga agtaaaaata gcttagaaag ctctgtcagg tgttttgtgc
     3001 agctgacaga ggtaatgtta catcacctaa aaaagaaaga tacacggtca gttatcctaa
     3061 aaataaattg tttggaaagt acaatgcacc acatttttgt agaagtctac tatttgataa
     3121 acagttgaaa ttcaagatgt gtttgaccct tagtcatttt tactctttgg ttctgagtat
     3181 acctattttc ttagcgtatc tgccttgttt atctttttct tcacctttta acaagtatga
     3241 cataggaaag tcattttttt ttagaattca tggatcagtc tgatctactc ttattcataa
     3301 tggaacatgt aaatatactg aaaactgttt ttcaggagag aaatatgagt tggagggaag
     3361 gaaaagtggt tctactaatg ttccaaaatc ctcatcagag aaggtatgat gttctcaggt
     3421 gtggaaaata ttttttagtt gattgagaat gcaggtttaa cagaagagat aaggggcata
     3481 atgactgctg gttttccaga ctggattttc ctaccgcaac tattaatgtt ctcagagttg
     3541 atgaggacca cctttgtgta tacacttgta gttttaaacc ttgcattggt aacaaaatga
     3601 tcaactttaa tccaggtaga attcaagatg gctgtacttc agttgtatga taaaattaat
     3661 ggttctcatg acttgtgtgg catctaaaaa taatgttttt atagcatctc tctgccacta
     3721 aattgttgac ttgaattttg ggaaaaaaaa aagttggtgt tgatatgtat atgtgtgtgt
     3781 gtatatatgt atttataaac aagtgtgttt gagtaacaag tgagtttcat agtcttcccc
     3841 tacgcatgtg tattccacac acaaatggct gagttatagt cataaaacaa tttgcaataa
     3901 aaaaaaaacc aaaacagatt gtcagttaac caggaaacag ttaatgtttt ttaatgaatc
     3961 tggcattata gtgagcaaat gtcgtattaa tttaggctaa tttctaatac taccataatt
     4021 tgtgtctaaa tttctgttgg ggtagaaatt actaaaattg tggggagttt tttctgattt
     4081 ttacattgct ttaggaaaca tttttactaa ttcagctgtc ttaggtaaaa tgaatagttt
     4141 tcttcctgtt tttttatgtg tcattgttag tggtctcaga attctgatca gtaactttgt
     4201 gtatgatgct gaattacaaa ccgtttgaat gatccagttg aaaacgtatc cctctacttt
     4261 cttcagttgt agaaaaggtt aatttccctc agtgtcccac attataccaa cctaagagaa
     4321 gaacaggtaa tagggagaaa taaacatacg gtggtttcag tggttttggt catgtgtcca
     4381 caggagaaac taaccattca gttgtcttaa ttttagttcg ttctaccctg tgaggagttt
     4441 gtttccatca gttgttgact ttccaaaatg ttgcattaag taatagttgt cactctgttg
     4501 gtctcatggt caatatcaat cagactttca tgatctctac taattattag tagagtcctg
     4561 tactatgtct gtaactacta agtttaaaga aaagcacata gtcacttcat ctcttttttt
     4621 cttagcctac gctcactccc caacccatcc caacattgac atgctatctg tggacaaata
     4681 gcagttctca gaatctagtc aagttgccat catccccctt gccttggccg ttcatagtag
     4741 gtatgcatat gtttgtttct gtacagtact gtgtgtgtgt gtgtgtatat atatatacat
     4801 ctgtatgcac acatctttga taaaatagct atttgactag cagggttaaa gtggctttta
     4861 attacttcgt gagtgttatt ggatacatct taaaaaaaaa aaatctgaac cagaaccatg
     4921 ccatacttgg ttgactattt tgagcattaa aattgcttta ctaattattt agacatgatc
     4981 acaattctgt atctttactg aggaaagatt cacgtaagct ctgaaaaatc ggattctttg
     5041 gcagattttc ctttgagtca agtgtctgaa atggagtgaa aatatatcct aactaaatta
     5101 atgtggaaag agcatttttt tagacaattt caattttaaa cacataaaac tttcaagatc
     5161 ttcaggactt tttaaagcac atttgaaatt attttagtaa gaattttgtt ttatcaatag
     5221 atgttgaatt ctgtttttta attaaataca aagcttagat ttcagaaaga gagggaaaat
     5281 agctggtggt cccagagtgt gctgctgtta attgtttaac aaaggggaaa atgtacataa
     5341 acagataaag ttaccataaa ttccatgaac ttaaatctgt gattcattgc cttaaaactt
     5401 tctctcttag aatttccata ccgcatgcca aaccagtaaa atggctttta aaaatgtata
     5461 gtagacaatg tcagtttgta taaaagtacc aagtgaaaat atttattaca tgcattggaa
     5521 aaaaattgtt tacctattga atgttacctg tttatgtaga gctctttaga tgtaataaaa
     5581 gaaaagcctt cagttaattt gtcttctgta aaataacact gctggatgtt caaggggaca
     5641 atccctatga gacaagtgat aatggtttgt gcttccaaag cacctttcat ccagagattt
     5701 caaagcatga caagtaacta actaattttc ttgcagcatg ctttgaggta agtaatgaat
     5761 atagccatca tttccctttc ttgttgaaat atggctaact ctttaataaa tcacagcatc
     5821 tgtttagtat ctgcagtttg aatgctaaga gcaccgtggc cttcttgtaa acaacactag
     5881 gtgctagaga aaccagctgg aatttcagga atgagcttga aaccaggacc aggagtaagg
     5941 taacagccct tcctcgggta gaggtttgaa tcaaacactt aataggatct tagactctgg
     6001 accactgaat cccttaatga gcagggagtc cgtgaagagg agccccaggt tctaatgacc
     6061 attccacacc aactgtgtgt ttttggcaag ttatatttca gattcctgtt aggcaaatga
     6121 gattcataaa acttgcttta aaaccatact gggactattc agtgataaat atagacatag
     6181 aaaagctttg acggaaagta ccctcattta ttatatccag aacttgctct tcctgtgcca
     6241 tctaaatcat tggtatctat ctcccatcta atctttggta ttccaggttg gctgtgcagg
     6301 atttacttcc taatgtccat gactggagct caaaagaact gtgggcctag gacctttgag
     6361 ctttgcctga aagtgacagc agttctctcg gcggggccag catctaaccg gccctctggt
     6421 ggatttacca tgaaggtaat gaagtttaag caccaggacc cttcacttgt tctggtccta
     6481 ggagcagccc cagaaattct gcatttctgt ttttgtattc ttaaggcagc caaatctcgt
     6541 aaacctcaga ccccacaaaa catctgctcc tacactgtgt ggacctgggc ttcccagcca
     6601 gatggtcaca acttcaccta gttggcattt gggccattta tttaccctca ccccacaaaa
     6661 ctctttggct cctaggaatt cctcctctcc ttgcagttat gcttggggaa atgagagtct
     6721 ccttcctcag ggccttgttt gggtctgaag ccacacagca tcgttgagac agttgcacta
     6781 acactacaac tcttgttccc tgcagttttc cctgtgcccg gtgcctagtg aaagatttgt
     6841 gtcttgtaca tcctcccaaa agacacatcc catttgattt ggttttcttc tactttagcc
     6901 actgtggtac tactagacaa agccacagcc cagagaaccc acttttgata catacatgga
     6961 tgcaaatctt tgtacttgaa gccctgcacc cctacctgtg ttggtgtggt actccagccc
     7021 ttttaggact gtgctggcac tacgaatgat ccagatttga caagtgtatg gcacctctga
     7081 atctctggca gcaccaacct ggcaccttct agctgtgtaa ctcactcaaa gccactccag
     7141 ctggatggac tcccagaaag cctagtgctc ctactactgt tttccagggc tgttgtaccc
     7201 tggccattgc tggttacatc cccttatttc tctccgtaag tcctggggag gtggcttaag
     7261 gcaatgacca gtgtgggcca cttgagcaga ccatcccgga acctctctgc ctgttaacag
     7321 agcccaggga acagctcgga attctcacag cattggaagc ccccttcttg agctctgcaa
     7381 ggccagtcaa tttagcagct cacatctggt ttcctttggt gtggggtgta aagtagatcg
     7441 ctcttcctgc tctcagctga tgctgcctca ttgtagcatg tgttttcact ctagtgtttt
     7501 cactctacac tctgtgttta tgaatgaata gcctgagcta tgtcactata tctgttgaga
     7561 tggaaatgag aatttgtaaa tgggcctctg gcactgcaag aattgttgtc ataagtgtta
     7621 cagctttctg ttcattgctg cttctcagct agcactggct tcatcttgga aaatcttgga
     7681 aatatggaag atggttctag cccccaaata cagaataagt ctcttggtag attgccctta
     7741 agtcatcagc tcaatccttc ctcaacaggc tccatgaggt aagaagtggg aatccagctt
     7801 gtgtgggggg gctttgagga gtgaaatgat ttgcccaaag tcacagtggg aagcagcaga
     7861 gccaggactg gaacccaggc cagactccaa accgcaggct ccttccatgc ttgaaagggc
     7921 cggactcacg gtagttaata aaatcaatac aaatctttta ttaaagatct actcatacca
//
