LOCUS       NM_018323               3594 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B),
            transcript variant 1, mRNA.
ACCESSION   NM_018323
VERSION     NM_018323.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Lopes da Silva,M., O'Connor,M.N., Kriston-Vizi,J., White,I.J.,
            Al-Shawi,R., Simons,J.P., Mossinger,J., Haucke,V. and Cutler,D.F.
  TITLE     Type II PI4-kinases control Weibel-Palade body biogenesis and von
            Willebrand factor structure in human endothelial cells
  JOURNAL   J Cell Sci 129 (10), 2096-2105 (2016)
   PUBMED   27068535
  REMARK    GeneRIF: RNA interference of the type II phosphatidylinositol
            4-kinases PI4KIIa and PI4KIIa in primary human endothelial cells
            leads to formation of an increased proportion of short WPB with
            perturbed packing of VWF, as exemplified by increased exposure of
            antibody-binding sites.
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Klima,M., Baumlova,A., Chalupska,D., Hrebabecky,H., Dejmek,M.,
            Nencka,R. and Boura,E.
  TITLE     The high-resolution crystal structure of phosphatidylinositol
            4-kinase IIbeta and the crystal structure of phosphatidylinositol
            4-kinase IIalpha containing a nucleoside analogue provide a
            structural basis for isoform-specific inhibitor design
  JOURNAL   Acta Crystallogr D Biol Crystallogr 71 (Pt 7), 1555-1563 (2015)
   PUBMED   26143926
  REMARK    GeneRIF: Here two crystal structures are presented: the structure
            of human PIK42A and the structure of PIK42B containing a nucleoside
            analogue.
REFERENCE   3  (bases 1 to 3594)
  AUTHORS   Sinha,R.K., Bojjireddy,N., Kanojia,D. and Subrahmanyam,G.
  TITLE     Type II PtdIns 4-kinase beta associates with CD4-p56lck complex and
            is involved in CD4 receptor signaling
  JOURNAL   Mol Cell Biochem 395 (1-2), 231-239 (2014)
   PUBMED   24972704
  REMARK    GeneRIF: Results provide evidence that type II PtdIns 4-kinase b
            was indeed recruited to CD4- p56lck complex upon CD4 receptor cross
            linking, suggests a mechanism for its association and a role for it
            in CD4-mediated intracellular calcium release.
REFERENCE   4  (bases 1 to 3594)
  AUTHORS   Bojjireddy,N., Sinha,R.K. and Subrahmanyam,G.
  TITLE     Type II phosphatidylinositol 4-kinases interact with
            FcepsilonRIgamma subunit in RBL-2H3 cells
  JOURNAL   Mol Cell Biochem 390 (1-2), 197-203 (2014)
   PUBMED   24481753
  REMARK    GeneRIF: Canonical tyrosine residues mutation in FcepsilonRIgamma
            ITAM (Y65 and Y76) reveals that these two tyrosine residues in
            gamma subunit are required for its interaction with type II PtdIns
            4-kinases.
REFERENCE   5  (bases 1 to 3594)
  AUTHORS   Sinha,R.K., Bojjireddy,N., Kulkarni,D., Ratheesh,A.,
            Chiplunkar,S.V., Gude,R. and Subrahmanyam,G.
  TITLE     Type II phosphatidylinositol 4-kinase beta is an integral signaling
            component of early T cell activation mechanisms
  JOURNAL   Biochimie 95 (8), 1560-1566 (2013)
   PUBMED   23619705
  REMARK    GeneRIF: type II PtdIns 4-kinase beta is a key component in early T
            cell activation signaling cascades
REFERENCE   6  (bases 1 to 3594)
  AUTHORS   Jones,D.R., Bultsma,Y., Keune,W.J., Halstead,J.R., Elouarrat,D.,
            Mohammed,S., Heck,A.J., D'Santos,C.S. and Divecha,N.
  TITLE     Nuclear PtdIns5P as a transducer of stress signaling: an in vivo
            role for PIP4Kbeta
  JOURNAL   Mol Cell 23 (5), 685-695 (2006)
   PUBMED   16949365
  REMARK    GeneRIF: UV irradiation increases nuclear PtdIns5P levels via
            inhibition of the activity of the beta isoform of PtdIns5P 4-kinase
            (PIP4Kbeta), an enzyme that can phosphorylate and remove PtdIns5P.
REFERENCE   7  (bases 1 to 3594)
  AUTHORS   Srivastava,R., Sinha,R.K. and Subrahmanyam,G.
  TITLE     Type II phosphatidylinositol 4-kinase beta associates with TCR-CD3
            zeta chain in Jurkat cells
  JOURNAL   Mol Immunol 43 (5), 454-463 (2006)
   PUBMED   16337488
  REMARK    GeneRIF: Type II PtdIns 4-kinase beta interacts with TCR-CD3 zeta
            chain. The C-terminal ITAM is critical for enzyme docking on the
            zeta chain. The association is tyrosyl phosphorylation dependent.
            Mutation of Y-151 & Y-142 disrupts interaction of the 2 proteins.
REFERENCE   8  (bases 1 to 3594)
  AUTHORS   Wei,Y.J., Sun,H.Q., Yamamoto,M., Wlodarski,P., Kunii,K.,
            Martinez,M., Barylko,B., Albanesi,J.P. and Yin,H.L.
  TITLE     Type II phosphatidylinositol 4-kinase beta is a cytosolic and
            peripheral membrane protein that is recruited to the plasma
            membrane and activated by Rac-GTP
  JOURNAL   J Biol Chem 277 (48), 46586-46593 (2002)
   PUBMED   12324459
REFERENCE   9  (bases 1 to 3594)
  AUTHORS   Huang,C., Handlogten,M.E. and Miller,R.T.
  TITLE     Parallel activation of phosphatidylinositol 4-kinase and
            phospholipase C by the extracellular calcium-sensing receptor
  JOURNAL   J Biol Chem 277 (23), 20293-20300 (2002)
   PUBMED   11907035
REFERENCE   10 (bases 1 to 3594)
  AUTHORS   Balla,A., Tuymetova,G., Barshishat,M., Geiszt,M. and Balla,T.
  TITLE     Characterization of type II phosphatidylinositol 4-kinase isoforms
            reveals association of the enzymes with endosomal vesicular
            compartments
  JOURNAL   J Biol Chem 277 (22), 20041-20050 (2002)
   PUBMED   11923287
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC051749.1, AY091514.1 and
            AC108218.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_018323.3.
            
            Summary: This gene encodes a member of the type II PI4 kinase
            protein family. The encoded protein is primarily cytosolic and
            contributes to overall PI4-kinase activity along with other protein
            family members. This protein is involved in early T cell
            activation. [provided by RefSeq, Dec 2016].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051749.1, AY091514.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264864.8/ ENSP00000264864.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-362               BC051749.1         3-364
            363-2938            AY091514.1         352-2927
            2939-3594           AC108218.3         5851-6506
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.2"
     gene            1..3594
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /note="phosphatidylinositol 4-kinase type 2 beta"
                     /db_xref="GeneID:55300"
                     /db_xref="HGNC:HGNC:18215"
                     /db_xref="MIM:612101"
     exon            1..399
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..1577
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /EC_number="2.7.1.67"
                     /note="phosphatidylinositol 4-kinase type-II beta;
                     PI4KII-BETA"
                     /codon_start=1
                     /product="phosphatidylinositol 4-kinase type 2-beta"
                     /protein_id="NP_060793.2"
                     /db_xref="CCDS:CCDS3433.1"
                     /db_xref="GeneID:55300"
                     /db_xref="HGNC:HGNC:18215"
                     /db_xref="MIM:612101"
                     /translation="MEDPSEPDRLASADGGSPEEEEDGEREPLLPRIAWAHPRRGAPG
                     SAVRLLDAAGEEGEAGDEELPLPPGDVGVSRSSSAELDRSRPAVSVTIGTSEMNAFLD
                     DPEFADIMLRAEQAIEVGIFPERISQGSSGSYFVKDPKRKIIGVFKPKSEEPYGQLNP
                     KWTKYVHKVCCPCCFGRGCLIPNQGYLSEAGAYLVDNKLHLSIVPKTKVVWLVSETFN
                     YNAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPKIGSFQLFVEGYKEAEYWLRKFEAD
                     PLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVRYEKQKCEKEIDHKESKWIDDE
                     EFLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKVPFSEEIRNLILPYISDMNFV
                     QDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVE
                     RSQGGSQGRIVHLSNSFTQTVNCRKPFFSSW"
     exon            400..554
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            555..755
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            756..887
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1041
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1109
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1209
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1343
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1403
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..3594
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3458..3463
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /note="hexamer: AATAAA"
     polyA_site      3482
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /note="major polyA site"
     regulatory      3538..3543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /note="hexamer: TATAAA"
     polyA_site      3556
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
     regulatory      3572..3577
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
                     /note="hexamer: ATTAAA"
     polyA_site      3594
                     /gene="PI4K2B"
                     /gene_synonym="PI4KIIB; PIK42B"
ORIGIN      
        1 aagcgtgccc gtgcgctggt gaggtggcgt ccgttctacc cggtcgctcc cgttccgcgc
       61 catgcagagc ccagtctctg gcacctggct gctctgatct ggtctcagcg cggagggagc
      121 agagggagtc catggaggat ccctccgagc ccgaccggtt ggcgtccgcg gacggcggga
      181 gcccggagga ggaggaggat ggggagcggg agccgctgct accgcggatc gcctgggccc
      241 acccgcggag aggcgcccca ggcagcgccg tgaggctgct ggacgctgcc ggggaggagg
      301 gcgaggccgg cgacgaggag ctgcccctcc cgcccgggga cgtgggggtc tcccggagtt
      361 cgtccgccga gctggaccgg agccgccccg cggtttcagt aactattggt acttcagaga
      421 tgaatgcatt cttggatgac ccagaatttg ccgatattat gctgagagca gagcaagcaa
      481 tagaagttgg aatttttcca gaaagaatct ctcaaggttc aagtggaagt tactttgtga
      541 aggatcctaa gaggaaaatt attggtgtgt ttaaacccaa atcagaagag ccttatggtc
      601 aactcaatcc aaaatggacc aaatatgtcc ataaggtctg ctgcccttgc tgctttggcc
      661 gaggctgcct gattcctaat caggggtacc tttccgaagc gggtgcctat cttgtggaca
      721 acaagcttca tctgagcatt gtacctaaaa caaaggtggt ttggcttgtc agtgagacat
      781 ttaactataa tgcgattgac cgtgcaaaat caagaggcaa aaagtatgct ttagaaaaag
      841 tgccaaaagt gggtagaaag tttcatagga taggactccc tcctaagatt ggttcctttc
      901 agttatttgt tgaaggttac aaggaggctg aatattggct taggaaattt gaagctgacc
      961 ctttgcctga gaatattaga aaacaatttc agtcacaatt tgaaagatta gttattttgg
     1021 attacatcat cagaaataca gacaggggca atgataattg gttagtcaga tacgaaaagc
     1081 agaaatgtga aaaggaaatt gaccataagg aatcaaaatg gattgatgat gaagaattcc
     1141 ttattaaaat agctgcaatt gataatggtc tagcatttcc ttttaaacat cctgatgaat
     1201 ggagagcata tccatttcac tgggcttggc ttcctcaagc aaaagttccc ttttctgaag
     1261 aaataagaaa tttgattcta ccatatattt ctgacatgaa ctttgtgcaa gatttatgtg
     1321 aagatctcta tgaacttttt aagactgaca aaggatttga caaagccact tttgaaagtc
     1381 agatgtctgt gatgaggggt cagatcttaa accttactca ggcattgaga gacgggaaga
     1441 gtcctttcca gctagtacag ataccttgtg tgattgtgga acgcagtcaa ggtggaagtc
     1501 agggtcggat tgtccacctg agcaattcct ttacccagac tgtcaattgc aggaagccat
     1561 ttttttcctc ctggtagtaa atgtcagagt aagagaaaca aactgtttag aattatcatg
     1621 tttttaaaac atcatagtaa tataaatctg ctgttaggag ctccagttgc taaaacctca
     1681 atttaagtct ttaaaaggtt gtattttgaa tgtaaccaaa agtttacagt tttttgtcca
     1741 aatattaaat ttctatttca gggaagaagt gctatatctc ctatattgta tttttgtaga
     1801 aaatttgtat tttatgttgt tgttagttta aaaggtaatt ttacacatgc tggaatgact
     1861 gtaattactc tagaattcca agtagaatac aataactttt aatattgaga agaatgttca
     1921 tgctaattct tcttacatta caaaaggcct ttgaggatgc ctacgtctga aattgctctt
     1981 acgaacttta ataaaatagt tagctaatag aaaaacaggt aagaataaag caatgttgcc
     2041 ttaatttcaa aagctgctat tttagaattt gaataagtac tcctaaagtg accattatta
     2101 gggaccagaa aattatatct tggctaagta atagaggacc attttggttt ttgtacttga
     2161 gaatattttt ggtgaattac tttgttgtag tgaggaaaaa acctaagaaa tttccccttt
     2221 ttttaaaaaa tggaaatatt caattgagac ttgaggggaa taatagaaaa ttaaggtaga
     2281 tccccaatat tttggaatac caaaattgcc ttaaaaattc ccttctgttt cttacatggg
     2341 atcaaatact tgagattagt acttcagagt actggccttg ttcaatttag tacttcaatt
     2401 agtattaaac ttcactaaaa agtaaaccat actccaaatt gtatattgga ttgcattttg
     2461 gggtcctagg tcatacgttc ttcaaaatta ttatgattgt actattgtac ttgaaattac
     2521 agatgttatt ataattacag tcaaatgtag actatcaggc caaattaaag gggagcatgg
     2581 cagataacca taaagtcatt tatatttgat tttgaaatgt atttttgtac tttattttga
     2641 atatcatcca tatgtctgac attattggaa tttgtaacat tgttaatgca ctaagtgatt
     2701 taaattcaat tgatgaagat gtgattttac agaagcagaa gtttcatttt cttgaggctt
     2761 aaaaccaatg tcaccacttg ggcttaactg ggtaatttgt ggtctaggcc ttttgttttc
     2821 taagcttact atcttgtgtt tgtttatttg cttttaatga agtattttgt gtagaaggtt
     2881 aaattaggat gcaaaacaga tctgccattc ctttttccct tatatcttcc ttttggtctt
     2941 catggacgag atgaatgagg attctgctgc cctgagggag ttcattggaa acctgcgttc
     3001 tcctacctct tccaaccctc cattagctca attttgagat aatggaaaat tgactggaaa
     3061 ttcaaaactc aaactactat ctttagatat aaacactagt aattaaaatg tgccttttga
     3121 agatgttttc taagagaaag gaaatacgtt gcagtgatgt gggtactgct ttcataaaac
     3181 agttttttca gtattttgag aattgccata ttaattttta taatggaaaa cttaataaat
     3241 tgctactgtt ttatatcata aaattaaaat accatggtaa tatttgcaaa aggtctggcc
     3301 ataccagaaa agtacagttg agatagttaa gatataacca caagtcagag tacattggtt
     3361 gtattttgta aactttcatg aactgaattc ttatttaaat agtatggttt tttttcaata
     3421 agtatattta tagtgacaaa tgtggtagac taaaggtaat aaaaatcatt gtcttaagat
     3481 tatgttcttc tgtatgtacg tgtttggaat atttagaatg tttatgaaca catttactat
     3541 aaatgatgcc aaactatcat tttcttcata tattaaaggt atagtttaat tctt
//
