LOCUS       NM_153370               1885 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens peptidase inhibitor 16 (PI16), transcript variant 1,
            mRNA.
ACCESSION   NM_153370
VERSION     NM_153370.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Chen,Q., Jiang,L.Y., Cao,C., Liu,F.Y., Li,D.R., Wu,P.F. and
            Jiang,K.R.
  TITLE     Peptidase inhibitor 16 promotes proliferation of pancreatic ductal
            adenocarcinoma cells through OASL signaling
  JOURNAL   Mol Carcinog 63 (5), 938-950 (2024)
   PUBMED   38353288
  REMARK    GeneRIF: Peptidase inhibitor 16 promotes proliferation of
            pancreatic ductal adenocarcinoma cells through OASL signaling.
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wang,F., Gu,X., Lin,S., Wu,Q., Sun,Y., Zhang,Q., Luo,A., Feng,X.,
            Wang,L., Xu,L., Sun,W. and Tan,W.
  TITLE     Peptidase inhibitor 16 promotes inflammatory arthritis by
            suppressing Foxp3 expression via regulating K48-linked ubiquitin
            degradation Bmi-1 in regulatory T cells
  JOURNAL   Clin Immunol 259, 109883 (2024)
   PUBMED   38147957
  REMARK    GeneRIF: Peptidase inhibitor 16 promotes inflammatory arthritis by
            suppressing Foxp3 expression via regulating K48-linked ubiquitin
            degradation Bmi-1 in regulatory T cells.
REFERENCE   3  (bases 1 to 1885)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1885)
  AUTHORS   Hope,C.M., Welch,J., Mohandas,A., Pederson,S., Hill,D.,
            Gundsambuu,B., Eastaff-Leung,N., Grosse,R., Bresatz,S., Ang,G.,
            Papademetrios,M., Zola,H., Duhen,T., Campbell,D., Brown,C.Y.,
            Krumbiegel,D., Sadlon,T., Couper,J.J. and Barry,S.C.
  TITLE     Peptidase inhibitor 16 identifies a human regulatory T-cell subset
            with reduced FOXP3 expression over the first year of recent onset
            type 1 diabetes
  JOURNAL   Eur J Immunol 49 (8), 1235-1250 (2019)
   PUBMED   31127857
  REMARK    GeneRIF: this study shows that Peptidase inhibitor 16 identifies a
            human regulatory T-cell subset with reduced FOXP3 expression over
            the first year of recent onset type 1 diabetes
REFERENCE   5  (bases 1 to 1885)
  AUTHORS   Lupsa,N., Ersek,B., Horvath,A., Bencsik,A., Lajko,E., Sillo,P.,
            Oszvald,A., Wiener,Z., Remenyi,P., Mikala,G., Masszi,T., Buzas,E.I.
            and Pos,Z.
  TITLE     Skin-homing CD8+ T cells preferentially express GPI-anchored
            peptidase inhibitor 16, an inhibitor of cathepsin K
  JOURNAL   Eur J Immunol 48 (12), 1944-1957 (2018)
   PUBMED   30365157
  REMARK    GeneRIF: this study shows that skin-homing CD8(+) T cells
            preferentially express GPI-anchored peptidase inhibitor 16
REFERENCE   6  (bases 1 to 1885)
  AUTHORS   Nicholson,I.C., Mavrangelos,C., Bird,D.R., Bresatz-Atkins,S.,
            Eastaff-Leung,N.G., Grose,R.H., Gundsambuu,B., Hill,D.,
            Millard,D.J., Sadlon,T.J., To,S., Zola,H., Barry,S.C. and
            Krumbiegel,D.
  TITLE     PI16 is expressed by a subset of human memory Treg with enhanced
            migration to CCL17 and CCL20
  JOURNAL   Cell Immunol 275 (1-2), 12-18 (2012)
   PUBMED   22533972
  REMARK    GeneRIF: PI16-positive Treg showed enhanced in vitro migration
            towards the inflammatory chemokines CCL17 and CCL20, suggesting
            they can migrate to sites of inflammation
REFERENCE   7  (bases 1 to 1885)
  AUTHORS   Bozaoglu,K., Curran,J.E., Stocker,C.J., Zaibi,M.S., Segal,D.,
            Konstantopoulos,N., Morrison,S., Carless,M., Dyer,T.D., Cole,S.A.,
            Goring,H.H., Moses,E.K., Walder,K., Cawthorne,M.A., Blangero,J. and
            Jowett,J.B.
  TITLE     Chemerin, a novel adipokine in the regulation of angiogenesis
  JOURNAL   J Clin Endocrinol Metab 95 (5), 2476-2485 (2010)
   PUBMED   20237162
REFERENCE   8  (bases 1 to 1885)
  AUTHORS   Reeves,J.R., Dulude,H., Panchal,C., Daigneault,L. and Ramnani,D.M.
  TITLE     Prognostic value of prostate secretory protein of 94 amino acids
            and its binding protein after radical prostatectomy
  JOURNAL   Clin Cancer Res 12 (20 Pt 1), 6018-6022 (2006)
   PUBMED   17062675
  REMARK    GeneRIF: a novel and independent prognostic markers following
            radical prostatectomy for prostate cancer.
REFERENCE   9  (bases 1 to 1885)
  AUTHORS   Reeves,J.R., Xuan,J.W., Arfanis,K., Morin,C., Garde,S.V.,
            Ruiz,M.T., Wisniewski,J., Panchal,C. and Tanner,J.E.
  TITLE     Identification, purification and characterization of a novel human
            blood protein with binding affinity for prostate secretory protein
            of 94 amino acids
  JOURNAL   Biochem J 385 (Pt 1), 105-114 (2005)
   PUBMED   15344909
  REMARK    GeneRIF: A new blood protein PSP94-binding protein (PSPBP) is
            identified that binds prostate secretory protein of 94 amino acids
            (PSP94).
REFERENCE   10 (bases 1 to 1885)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC035634.1, BC022399.1 and AL050088.1.
            
            On Nov 22, 2018 this sequence version replaced NM_153370.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.248262.1, BC022399.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373674.4/ ENSP00000362778.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-170               BC035634.1         306-475
            171-1222            BC022399.1         170-1221
            1223-1598           BC035634.1         1528-1903
            1599-1879           BC022399.1         1599-1879
            1880-1885           AL050088.1         601-606
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.2"
     gene            1..1885
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /note="peptidase inhibitor 16"
                     /db_xref="GeneID:221476"
                     /db_xref="HGNC:HGNC:21245"
     exon            1..194
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1415
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /note="protease inhibitor 16; microseminoprotein,
                     beta-binding protein; PSP94-binding protein; cysteine-rich
                     secretory protein 9"
                     /codon_start=1
                     /product="peptidase inhibitor 16 precursor"
                     /protein_id="NP_699201.2"
                     /db_xref="CCDS:CCDS34440.1"
                     /db_xref="GeneID:221476"
                     /db_xref="HGNC:HGNC:21245"
                     /translation="MHGSCSFLMLLLPLLLLLVATTGPVGALTDEEKRLMVELHNLYR
                     AQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLA
                     MEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIEL
                     LVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPS
                     FRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSM
                     ATEAPPCVTTEVPSILAAHSLPSLDEEPVTFPKSTHVPIPKSADKVTDKTKVPSRSPE
                     NSLDPKMSLTGARELLPHAQEEAEAEAELPPSSEVLASVFPAQDKPGELQATLDHTGH
                     TSSKSLPNFPNTSATANATGGRALALQSSLPGAEGPDKPSVVSGLNSGPGHVWGPLLG
                     LLLLPPLVLAGIF"
     exon            195..416
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /inference="alignment:Splign:2.1.0"
     exon            417..526
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /inference="alignment:Splign:2.1.0"
     exon            527..615
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /inference="alignment:Splign:2.1.0"
     exon            616..1293
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1433
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1885
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1859..1864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /note="hexamer: AATAAA"
     polyA_site      1885
                     /gene="PI16"
                     /gene_synonym="CD364; CRISP9; MSMBBP; PSPBP"
                     /note="major polyA site"
ORIGIN      
        1 agaaggagag acggctggcc accatgcacg gctcctgcag tttcctgatg cttctgctgc
       61 cgctactgct actgctggtg gccaccacag gccccgttgg agccctcaca gatgaggaga
      121 aacgtttgat ggtggagctg cacaacctct accgggccca ggtatccccg acggcctcag
      181 acatgctgca catgagatgg gacgaggagc tggccgcctt cgccaaggcc tacgcacggc
      241 agtgcgtgtg gggccacaac aaggagcgcg ggcgccgcgg cgagaatctg ttcgccatca
      301 cagacgaggg catggacgtg ccgctggcca tggaggagtg gcaccacgag cgtgagcact
      361 acaacctcag cgccgccacc tgcagcccag gccagatgtg cggccactac acgcaggtgg
      421 tatgggccaa gacagagagg atcggctgtg gttcccactt ctgtgagaag ctccagggtg
      481 ttgaggagac caacatcgaa ttactggtgt gcaactatga gcctccgggg aacgtgaagg
      541 ggaaacggcc ctaccaggag gggactccgt gctcccaatg tccctctggc taccactgca
      601 agaactccct ctgtgaaccc atcggaagcc cggaagatgc tcaggatttg ccttacctgg
      661 taactgaggc cccatccttc cgggcgactg aagcatcaga ctctaggaaa atgggtactc
      721 cttcttccct agcaacgggg attccggctt tcttggtaac agaggtctca ggctccctgg
      781 caaccaaggc tctgcctgct gtggaaaccc aggccccaac ttccttagca acgaaagacc
      841 cgccctccat ggcaacagag gctccacctt gcgtaacaac tgaggtccct tccattttgg
      901 cagctcacag cctgccctcc ttggatgagg agccagttac cttccccaaa tcgacccatg
      961 ttcctatccc aaaatcagca gacaaagtga cagacaaaac aaaagtgccc tctaggagcc
     1021 cagagaactc tctggacccc aagatgtccc tgacaggggc aagggaactc ctaccccatg
     1081 cccaggagga ggctgaggct gaggctgagt tgcctccttc cagtgaggtc ttggcctcag
     1141 tttttccagc ccaggacaag ccaggtgagc tgcaggccac actggaccac acggggcaca
     1201 cctcctccaa gtccctgccc aatttcccca atacctctgc caccgctaat gccacgggtg
     1261 ggcgtgccct ggctctgcag tcgtccttgc caggtgcaga gggccctgac aagcctagcg
     1321 tcgtgtcagg gctgaactcg ggccctggtc atgtgtgggg ccctctcctg ggactactgc
     1381 tcctgcctcc tctggtgttg gctggaatct tctgaagggg ataccactca aagggtgaag
     1441 aggtcagctg tcctcctgtc atcttcccca ccctgtcccc agcccctaaa caagatactt
     1501 cttggttaag gccctccgga agggaaaggc tacggggcat gtgcctcatc acaccatcca
     1561 tcctggaggc acaaggcctg gctggctgcg agctcaggag gccgcctgag gactgcacac
     1621 cgggcccaca cctctcctgc ccctccctcc tgagtcctgg gggtgggagg atttgaggga
     1681 gctcactgcc tacctggcct ggggctgtct gcccacacag catgtgcgct ctccctgagt
     1741 gcctgtgtag ctggggatgg ggattcctag gggcagatga aggacaagcc ccactggagt
     1801 ggggttcttt gagtggggga ggcagggacg agggaaggaa agtaactcct gactctccaa
     1861 taaaaacctg tccaacctgt ggcaa
//
