LOCUS       NM_015886               6735 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens peptidase inhibitor 15 (PI15), transcript variant 1,
            mRNA.
ACCESSION   NM_015886
VERSION     NM_015886.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Jiang,Y., Zheng,X., Jiao,D., Chen,P., Xu,Y., Wei,H. and Qian,Y.
  TITLE     Peptidase inhibitor 15 as a novel blood diagnostic marker for
            cholangiocarcinoma
  JOURNAL   EBioMedicine 40, 422-431 (2019)
   PUBMED   30638862
  REMARK    GeneRIF: PI15 as a novel marker for predicting the diagnosis and
            follow-up of cholangiocarcinoma patients.
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Prusty,B.K., Chowdhury,S.R., Gulve,N. and Rudel,T.
  TITLE     Peptidase Inhibitor 15 (PI15) Regulates Chlamydial CPAF Activity
  JOURNAL   Front Cell Infect Microbiol 8, 183 (2018)
   PUBMED   29900129
  REMARK    GeneRIF: Human serine peptidase inhibitor PI15 as a potential host
            factor involved in the regulation of CPAF activation. Silencing
            expression as well as over expression of PI15 affected normal
            development of Chlamydia.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6735)
  AUTHORS   Melzer,D., Perry,J.R., Hernandez,D., Corsi,A.M., Stevens,K.,
            Rafferty,I., Lauretani,F., Murray,A., Gibbs,J.R., Paolisso,G.,
            Rafiq,S., Simon-Sanchez,J., Lango,H., Scholz,S., Weedon,M.N.,
            Arepalli,S., Rice,N., Washecka,N., Hurst,A., Britton,A., Henley,W.,
            van de Leemput,J., Li,R., Newman,A.B., Tranah,G., Harris,T.,
            Panicker,V., Dayan,C., Bennett,A., McCarthy,M.I., Ruokonen,A.,
            Jarvelin,M.R., Guralnik,J., Bandinelli,S., Frayling,T.M.,
            Singleton,A. and Ferrucci,L.
  TITLE     A genome-wide association study identifies protein quantitative
            trait loci (pQTLs)
  JOURNAL   PLoS Genet 4 (5), e1000072 (2008)
   PUBMED   18464913
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6735)
  AUTHORS   Kaplan,F., Ledoux,P., Kassamali,F.Q., Gagnon,S., Post,M.,
            Koehler,D., Deimling,J. and Sweezey,N.B.
  TITLE     A novel developmentally regulated gene in lung mesenchyme: homology
            to a tumor-derived trypsin inhibitor
  JOURNAL   Am J Physiol 276 (6), L1027-L1036 (1999)
   PUBMED   10362728
REFERENCE   5  (bases 1 to 6735)
  AUTHORS   Yamakawa,T., Miyata,S., Ogawa,N., Koshikawa,N., Yasumitsu,H.,
            Kanamori,T. and Miyazaki,K.
  TITLE     cDNA cloning of a novel trypsin inhibitor with similarity to
            pathogenesis-related proteins, and its frequent expression in human
            brain cancer cells
  JOURNAL   Biochim Biophys Acta 1395 (2), 202-208 (1998)
   PUBMED   9473672
REFERENCE   6  (bases 1 to 6735)
  AUTHORS   Koshikawa,N., Nakamura,T., Tsuchiya,N., Isaji,M., Yasumitsu,H.,
            Umeda,M. and Miyazaki,K.
  TITLE     Purification and identification of a novel and four known serine
            proteinase inhibitors secreted by human glioblastoma cells
  JOURNAL   J Biochem 119 (2), 334-339 (1996)
   PUBMED   8882727
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026616.10, CR749657.1,
            D45027.1 and AC011632.7.
            
            On Nov 22, 2018 this sequence version replaced NM_015886.4.
            
            Summary: This gene encodes a trypsin inhibitor. The protein shares
            similarity to insect venom allergens, mammalian testis-specific
            proteins and plant pathogenesis-related proteins. It is frequently
            expressed in human neuroblastoma and glioblastoma cell lines, and
            thus may play a role in the central nervous system. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR749657.1, SRR1803611.259258.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260113.7/ ENSP00000260113.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 AC026616.10        157421-157423
            4-1020              CR749657.1         2-1018
            1021-1131           D45027.1           1323-1433
            1132-6735           AC011632.7         73887-79490
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q21.13"
     gene            1..6735
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /note="peptidase inhibitor 15"
                     /db_xref="GeneID:51050"
                     /db_xref="HGNC:HGNC:8946"
                     /db_xref="MIM:607076"
     exon            1..142
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /inference="alignment:Splign:2.1.0"
     exon            143..455
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..959
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /note="25 kDa trypsin inhibitor; protease inhibitor 15;
                     PI-15; CRISP-8; sugarCrisp; cysteine-rich secretory
                     protein 8"
                     /codon_start=1
                     /product="peptidase inhibitor 15 preproprotein"
                     /protein_id="NP_056970.1"
                     /db_xref="CCDS:CCDS6218.1"
                     /db_xref="GeneID:51050"
                     /db_xref="HGNC:HGNC:8946"
                     /db_xref="MIM:607076"
                     /translation="MIAISAVSSALLFSLLCEASTVVLLNSTDSSPPTNNFTDIEAAL
                     KAQLDSADIPKARRKRYISQNDMIAILDYHNQVRGKVFPPAANMEYMVWDENLAKSAE
                     AWAATCIWDHGPSYLLRFLGQNLSVRTGRYRSILQLVKPWYDEVKDYAFPYPQDCNPR
                     CPMRCFGPMCTHYTQMVWATSNRIGCAIHTCQNMNVWGSVWRRAVYLVCNYAPKGNWI
                     GEAPYKVGVPCSSCPPSYGGSCTDNLCFPGVTSNYLYWFK"
     exon            456..574
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /inference="alignment:Splign:2.1.0"
     exon            575..707
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /inference="alignment:Splign:2.1.0"
     exon            708..823
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /inference="alignment:Splign:2.1.0"
     exon            824..6735
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6717..6722
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /note="hexamer: AATAAA"
     polyA_site      6735
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /note="major polyA site"
ORIGIN      
        1 agcagcattc aaaccttgca gagctttgct ctcagagagt ttgtaaaaag acacactcct
       61 cttacaagag ttcatgctac cacatagcaa agaaccttaa atttttggaa gaacaatata
      121 ttcattttgg cattgtgcag agcaaagtaa actcggtggc ctcttcttct ccacccctca
      181 aaatgatagc aatctctgcc gtcagcagtg cactcctgtt ctcccttctc tgtgaagcaa
      241 gtaccgtcgt cctactcaat tccactgact catccccgcc aaccaataat ttcactgata
      301 ttgaagcagc tctgaaagca caattagatt cagcggatat ccccaaagcc aggcggaagc
      361 gctacatttc gcagaatgac atgatcgcca ttcttgatta tcataatcaa gttcggggca
      421 aagtgttccc accggcagca aatatggaat atatggtttg ggatgaaaat cttgcaaaat
      481 cggcagaggc ttgggcggct acttgcattt gggaccatgg accttcttac ttactgagat
      541 ttttgggcca aaatctatct gtacgcactg gaagatatcg ctctattctc cagttggtca
      601 agccatggta tgatgaagtg aaagattatg cttttccata tccccaggat tgcaacccca
      661 gatgtcctat gagatgtttt ggtcccatgt gcacacatta tacgcagatg gtttgggcca
      721 cttccaatcg gataggatgc gcaattcata cttgccaaaa catgaatgtt tggggatctg
      781 tgtggcgacg tgcagtttac ttggtatgca actatgcccc aaagggcaat tggattggag
      841 aagcaccata taaagtaggg gtaccatgtt catcttgtcc tccaagttat gggggatctt
      901 gtactgacaa tctgtgtttt ccaggagtta cgtcaaacta cctgtactgg tttaaataag
      961 tttacctttt cctccaggaa atataatgat ttctgggaac atgggcatgt atatatatat
     1021 atggagagag aattttgcac atattataca tattttgtgc taatcttgtt ttcctcttag
     1081 tattcctttg tataaattag tgtttgtcta gcatgtttgt ttaatccttt gaaatatttg
     1141 aaacatcaat ttctattttc tgacctctaa gcctaaatta agatattgta tatgtaatga
     1201 tgacatagtt gatgcatcca atcctaaaac ttacattcca aaggaattat atcattatgt
     1261 tcctaaggag taaatatata tttgacctgt aagtgtgtgt atgtatacat atacatatgt
     1321 atgtgtatgg atttatatat gcacacaaac atataatatg tgatgtaaca tgtagatgat
     1381 aatatgattc agtagtcaac ttgagggaaa tttttaaaaa actattctca attatatacg
     1441 aggtgatggg acttcttaac acacatttct ataataccca tgaaatgata atttgtaaaa
     1501 taacacttag tgatatctgg aaataataat tcaattaagc aaccacgaat ttcaccctgg
     1561 agatattttt tcttatttga gtccaccaaa ggataatgcc aacttatata agttctcaaa
     1621 tcatgccttc cgcttagtct cattttattc attcagtcgt catgagttga gtgcttacta
     1681 catgcaaggc actctgctag ttatattcta ataatgcaga gataattaga catggttccc
     1741 gccctcaaga agctcacaaa agtattcagg aaataatgca gactagtgat tttgctataa
     1801 aattattttt gaaggaagca gacacagcag tatttacctg taggtggagc aagtaataag
     1861 ccatgctgtg caatatatac ataaagcttc tgcttctcat gggaatttag ttacagtgct
     1921 tggaatgaga aggggaagga aagaattaac aaatgccaag atttctggag cagattgtac
     1981 agctgtgact ttggaaaaca gaaagtaaga ccctcagaaa accaatgaag tctaagagaa
     2041 ataaaattta gtggacaggt atgaaaagtg taattgcgcc taactaccag atggagagct
     2101 tcagaatggg ctatccttag agtctagtac atcttgaggc ctctcagcag gagacaaagg
     2161 attccaaaaa gagatgtgga ggtgctgagg gcacctctat ctctttgttg tttagtctgt
     2221 cattgaatca atctacttat catcttgggt ctttgagtat tgtatgaaag atccttcgtg
     2281 cacaccacac acagtcatga ttttgttaaa gtagccttct tcagatgctt ttctaagggc
     2341 tagttaccaa ctttatttct gtgtttctgt agaagaaaca ttttcagttc ttcattgagt
     2401 ttgattatgg aaatccattc aaagtcacta tgaaaatttt actcatgtag tttggaaatg
     2461 caacattttc ctatcatgaa atctctttca gagaggagaa tacaacatct tagtccagac
     2521 atttaacata ctgcatttca agtacatgtg tgtgtgtttt attcaggttg tgtaatgctc
     2581 ccgtagaatt atagaacaat taaatatggt tagttccaga gtgcaaatta cagaaggaag
     2641 ctacttgttt aaaattccat acacgtttgc agtttcttgt acacatttgg atactttgaa
     2701 agatgacaga ttgttaaatc cattcaatgg taaagaaact caccatctgg agattgagtc
     2761 tacttgttaa tgaatgacta gcccaattat ccttataaat tgaatatggt gaccaaatgc
     2821 tttgatatca tactactctg cctttgtggg cacatatgta gacactacta aaaataaata
     2881 tttttggaga ttaaaatgga gaatagaagt aattacatta tttaggtctt aatccaactt
     2941 ttttctaata tatctaaaca attgaaaggg aagcttattc atggaatatt ggcttgattt
     3001 atctagaaag tttttccttc ttcaatttta ctatattcat tctacaggaa cagcaataag
     3061 tactattaaa cagaagatgg ctacactaag ttccaatttt gttgctgaat tgcttctgtg
     3121 agttcacttt tcagttctaa ggaagaataa tatttgctac atatttcaca ggggttctta
     3181 tgaaggtaaa tttaccagat taataaaaat ttatgaatat taaaattatc attaataata
     3241 taaaacactt atttgagatt aaattaaatt tttcatgagc ccctctttgg caggaactct
     3301 gtttaattct ttgtatttat cccagcttct taaatggtgg ctgtaacata ataaatgttt
     3361 aataaatgct tatatgaatg gatttttaga attaactaag aagccaaaaa tggcaacaat
     3421 ttacagaaat cccacctttc catgcttaag acaaaaatgt cttaaatata aagctgtgat
     3481 tatatcaaaa atccagataa atcatcaaat atatcagatt aagaccaggg tttacacact
     3541 taggcaatag tcctttccaa accatgacaa aaactacaag tttatttata atttaacaac
     3601 tcagctgaaa atataacggg tatatttgtt attctaactc tattttttaa agttaataat
     3661 ataaagtggc catgtaaaat attttatttt ccaggctaaa gcaaatgaaa gtttgctggt
     3721 atcaacacag cctgccatat ttttcacagc atgcaacaat ggtgctagga tagctatttc
     3781 ttactgtaat tgccagaggc agaaatggtc tgggtataag ctatttcata aaagcagctt
     3841 taaattgtca gtattaaggt tttcatgtgg aaaggtgtca ttcaaaaaaa aagtaattgg
     3901 catacatatt ccacatcatc gatcctctct gtggtgttaa tttttttata tgaccagtag
     3961 aaaaatttta atattctcac aatataggtt ttggggcttc catatcatca aaagactgaa
     4021 aaattataat tttagaatta aactgatgga tttcattata gaattatctg tgagttgtgt
     4081 agacacagtc ttaatgtttc tggttatgac agataagttt gctcaaaaaa tgtggatgaa
     4141 gccattattg ttattattgt tattgcttct gttcagttgt ctaagtatca tcccttctgt
     4201 ggcccatcac gcagcagagt tgccctacaa atttcatttg gcagcgccat aacattcatt
     4261 taaaaagttt atgaaaacat tcatttgaaa gttccatgca gctttagcac agagttgacc
     4321 aaacactggc gtaagttcaa tttacacaga atatttgaat tgaaacaata gaaatttttc
     4381 tcataatata tacctatgtg aaaccaactt atctgcataa ttaaatctaa tacatattta
     4441 agccagttta agtgctttgt gttgatgcca tgcttatcaa atacatgcac aagctaaaca
     4501 taatttgaat gggtctatga aggaaaaata atgcttagac tttggtgtag gttcttcctg
     4561 tgtagccata tacccaggct ctgcagtatc gaaggatgca aatgttgaca tagatggaag
     4621 ctcttaccta ccaaagtgtt taggaaggat aaagttacat ttgtcttaat ttctaacatt
     4681 atctttgctt ttatgtttca taaaaatttg tcattattta tgctggtgaa acgtataatc
     4741 acatccaatt atttgaacac atgcaaaata attttttaaa ttatgttatt gtttaaattt
     4801 gacttatggg agatcagtca aaaacttaga aggtttaaca cttcactgat taatggtgct
     4861 gaaaacacgt tacaattacc acatatcctt gctataagtt ttgaagtttc ttagcaatta
     4921 aagttttttt attcagtgtg aactgtcagt atctattctg gtgctaaatg tatggtgcta
     4981 aatgaattgt tagtgttgat ggctttagta atgctccttt tattcattgc taaatttagt
     5041 gttatccatt tgattcctga ttcagaaata tcaataaaat cctatgttaa attaatcttt
     5101 accaaaaaca ggcaagttaa ctctgttgtt ttaattcaac agtccaacat tatttaggtg
     5161 ttacagagtg taaatatatt tctttgggag ttattttctt tttaaaatct ttttatagct
     5221 tggcaatgtc caaagtcaaa tatcacctaa actggttaga ttacttctac agctaataat
     5281 attgcaggca ctggcgccct ctggtggtta tgaagacaaa ttcttaatgg ctacttgacc
     5341 tacagcaaaa gccatttctg taccataaaa atttgttgtg caatattaga attatcatat
     5401 gtttcctaca tctgacagca cctaaaatgt ttgataatat taacatgtat ctaagaggaa
     5461 aaaagagtta atatattctg gcacccactt tcctagtaat gttttccatg attttccagt
     5521 tctgaggcac ttattaaagt gctttttttt ttctgaatta attaggtatt ggtaaaatat
     5581 atttttaaat ttagttagct ttataaacac aattagaatt acaattaatt aacagaggta
     5641 taattgtctc actttcagaa gtgatcattt atttttattt agcacaggtc ataagaaaaa
     5701 tatatagaaa aataatcaat ttcatatata aaaggattat ttctccacct ttaattattg
     5761 gcctatcatt tgttagtgtt atttggtcat attattgaac taatgtatta ttccattcaa
     5821 agtctttcta gatttaaaaa tgtatgcaaa agcttaggat tatatcatgt gtaactatta
     5881 tagataacat cctaaacctt cagtttagat atataattga ctgggtgtaa tctcttttgt
     5941 aatctgtttt gacagatttc ttaaattatg ttagcataat caaggaagat ttaccttgaa
     6001 gcactttcca aattgatact ttcaaactta ttttaaagca gtagaacctt ttctatgaac
     6061 taaatcacat gcaaaactcc aacctgtagt atacataaaa tggacttact tattcctctc
     6121 accttctcca gtgcctagga atattcttct ctgagcccta ggattgattc tatcacacag
     6181 agcaacatta atctaaatgg tttagctccc tcttttttct ctaaaaacaa tcagctaata
     6241 aaaaaaaaat ttgagggcct aaattatttc aatggttgtt tgaaatattc agttcagttt
     6301 gtacctgtta gcagtctttc agtttggggg agaattaaat actgtgctaa gctggtgctt
     6361 ggatacatat tacagcatct tgtgttttat ttgacaaaca gaattttggt gccataatat
     6421 tttgagaatt agagaagatt gtgatgcata tatataaaca ctatttttaa aaaatatcta
     6481 aatatgtctc acatatttat ataatcctca aatatactgt accattttag atatttttta
     6541 aacagattaa tttggagaag ttttattcat tacctaattc tgtggcaaaa atggtgcctc
     6601 tgatgttgtg atatagtatt gtcagtgtgt acatatataa aacctgtgta aacctctgtc
     6661 cttatgaacc ataacaaatg tagcttttta aagtccattg tattgttttt tctttcaata
     6721 aaagagtata attaa
//
