LOCUS       NP_056970                258 aa            linear   PRI 28-APR-2025
DEFINITION  peptidase inhibitor 15 preproprotein [Homo sapiens].
ACCESSION   NP_056970
VERSION     NP_056970.1
DBSOURCE    REFSEQ: accession NM_015886.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 258)
  AUTHORS   Jiang,Y., Zheng,X., Jiao,D., Chen,P., Xu,Y., Wei,H. and Qian,Y.
  TITLE     Peptidase inhibitor 15 as a novel blood diagnostic marker for
            cholangiocarcinoma
  JOURNAL   EBioMedicine 40, 422-431 (2019)
   PUBMED   30638862
  REMARK    GeneRIF: PI15 as a novel marker for predicting the diagnosis and
            follow-up of cholangiocarcinoma patients.
REFERENCE   2  (residues 1 to 258)
  AUTHORS   Prusty,B.K., Chowdhury,S.R., Gulve,N. and Rudel,T.
  TITLE     Peptidase Inhibitor 15 (PI15) Regulates Chlamydial CPAF Activity
  JOURNAL   Front Cell Infect Microbiol 8, 183 (2018)
   PUBMED   29900129
  REMARK    GeneRIF: Human serine peptidase inhibitor PI15 as a potential host
            factor involved in the regulation of CPAF activation. Silencing
            expression as well as over expression of PI15 affected normal
            development of Chlamydia.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 258)
  AUTHORS   Melzer,D., Perry,J.R., Hernandez,D., Corsi,A.M., Stevens,K.,
            Rafferty,I., Lauretani,F., Murray,A., Gibbs,J.R., Paolisso,G.,
            Rafiq,S., Simon-Sanchez,J., Lango,H., Scholz,S., Weedon,M.N.,
            Arepalli,S., Rice,N., Washecka,N., Hurst,A., Britton,A., Henley,W.,
            van de Leemput,J., Li,R., Newman,A.B., Tranah,G., Harris,T.,
            Panicker,V., Dayan,C., Bennett,A., McCarthy,M.I., Ruokonen,A.,
            Jarvelin,M.R., Guralnik,J., Bandinelli,S., Frayling,T.M.,
            Singleton,A. and Ferrucci,L.
  TITLE     A genome-wide association study identifies protein quantitative
            trait loci (pQTLs)
  JOURNAL   PLoS Genet 4 (5), e1000072 (2008)
   PUBMED   18464913
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 258)
  AUTHORS   Kaplan,F., Ledoux,P., Kassamali,F.Q., Gagnon,S., Post,M.,
            Koehler,D., Deimling,J. and Sweezey,N.B.
  TITLE     A novel developmentally regulated gene in lung mesenchyme: homology
            to a tumor-derived trypsin inhibitor
  JOURNAL   Am J Physiol 276 (6), L1027-L1036 (1999)
   PUBMED   10362728
REFERENCE   5  (residues 1 to 258)
  AUTHORS   Yamakawa,T., Miyata,S., Ogawa,N., Koshikawa,N., Yasumitsu,H.,
            Kanamori,T. and Miyazaki,K.
  TITLE     cDNA cloning of a novel trypsin inhibitor with similarity to
            pathogenesis-related proteins, and its frequent expression in human
            brain cancer cells
  JOURNAL   Biochim Biophys Acta 1395 (2), 202-208 (1998)
   PUBMED   9473672
REFERENCE   6  (residues 1 to 258)
  AUTHORS   Koshikawa,N., Nakamura,T., Tsuchiya,N., Isaji,M., Yasumitsu,H.,
            Umeda,M. and Miyazaki,K.
  TITLE     Purification and identification of a novel and four known serine
            proteinase inhibitors secreted by human glioblastoma cells
  JOURNAL   J Biochem 119 (2), 334-339 (1996)
   PUBMED   8882727
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026616.10, CR749657.1,
            D45027.1 and AC011632.7.
            
            Summary: This gene encodes a trypsin inhibitor. The protein shares
            similarity to insect venom allergens, mammalian testis-specific
            proteins and plant pathogenesis-related proteins. It is frequently
            expressed in human neuroblastoma and glioblastoma cell lines, and
            thus may play a role in the central nervous system. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR749657.1, SRR1803611.259258.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000260113.7/ ENSP00000260113.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q21.13"
     Protein         1..258
                     /product="peptidase inhibitor 15 preproprotein"
                     /note="25 kDa trypsin inhibitor; protease inhibitor 15;
                     PI-15; CRISP-8; sugarCrisp; cysteine-rich secretory
                     protein 8"
                     /calculated_mol_wt=27144
     sig_peptide     1..19
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=1939
     Site            26
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O43692.1)"
     Site            36
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O43692.1)"
     mat_peptide     61..258
                     /product="Peptidase inhibitor 15. /id=PRO_0000287623"
                     /note="propagated from UniProtKB/Swiss-Prot (O43692.1)"
                     /calculated_mol_wt=22720
     Region          67..212
                     /region_name="CAP_PI15"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of peptidase
                     inhibitor 15; cd18814"
                     /db_xref="CDD:349408"
     Site            124
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O43692.1)"
     CDS             1..258
                     /gene="PI15"
                     /gene_synonym="CRISP8; P24TI; P25TI"
                     /coded_by="NM_015886.5:183..959"
                     /db_xref="CCDS:CCDS6218.1"
                     /db_xref="GeneID:51050"
                     /db_xref="HGNC:HGNC:8946"
                     /db_xref="MIM:607076"
ORIGIN      
        1 miaisavssa llfsllceas tvvllnstds spptnnftdi eaalkaqlds adipkarrkr
       61 yisqndmiai ldyhnqvrgk vfppaanmey mvwdenlaks aeawaatciw dhgpsyllrf
      121 lgqnlsvrtg ryrsilqlvk pwydevkdya fpypqdcnpr cpmrcfgpmc thytqmvwat
      181 snrigcaiht cqnmnvwgsv wrravylvcn yapkgnwige apykvgvpcs scppsyggsc
      241 tdnlcfpgvt snylywfk
//
