LOCUS       NM_014172                577 bp    mRNA    linear   PRI 04-AUG-2024
DEFINITION  Homo sapiens phosphohistidine phosphatase 1 (PHPT1), transcript
            variant 3, mRNA.
ACCESSION   NM_014172
VERSION     NM_014172.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Zavala,E., Dansereau,S., Burke,M.J., Lipchock,J.M., Maschietto,F.,
            Batista,V. and Loria,J.P.
  TITLE     A salt bridge of the C-terminal carboxyl group regulates PHPT1
            substrate affinity and catalytic activity
  JOURNAL   Protein Sci 33 (6), e5009 (2024)
   PUBMED   38747379
  REMARK    GeneRIF: A salt bridge of the C-terminal carboxyl group regulates
            PHPT1 substrate affinity and catalytic activity.
REFERENCE   2  (bases 1 to 577)
  AUTHORS   Zhang,N., Liao,Y., Lv,W., Zhu,S., Qiu,Y., Chen,N., Xiao,M. and
            Zhang,H.
  TITLE     FBXO32 targets PHPT1 for ubiquitination to regulate the growth of
            EGFR mutant lung cancer
  JOURNAL   Cell Oncol (Dordr) 45 (2), 293-307 (2022)
   PUBMED   35411430
  REMARK    GeneRIF: FBXO32 targets PHPT1 for ubiquitination to regulate the
            growth of EGFR mutant lung cancer.
REFERENCE   3  (bases 1 to 577)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 577)
  AUTHORS   Cruz-Garcia,L., O'Brien,G., Donovan,E., Gothard,L., Boyle,S.,
            Laval,A., Testard,I., Ponge,L., Wozniak,G., Miszczyk,L.,
            Candeias,S.M., Ainsbury,E., Widlak,P., Somaiah,N. and Badie,C.
  TITLE     Influence of Confounding Factors on Radiation Dose Estimation Using
            In Vivo Validated Transcriptional Biomarkers
  JOURNAL   Health Phys 115 (1), 90-101 (2018)
   PUBMED   29787434
  REMARK    GeneRIF: High PHPT1 expression is associated with cancer.
REFERENCE   5  (bases 1 to 577)
  AUTHORS   McCullough,B.S. and Barrios,A.M.
  TITLE     Facile, Fluorogenic Assay for Protein Histidine Phosphatase
            Activity
  JOURNAL   Biochemistry 57 (18), 2584-2589 (2018)
   PUBMED   29630837
  REMARK    GeneRIF: In the process of assay optimization, we discovered that
            PHPT1 is sensitive to a reducing environment and inhibited by
            transition-metal ions, with one apparent Cu(II) binding site with
            IC50 value of 500 +/- 20 muM and two apparent Zn(II) binding sites
            with IC50 values of 25 +/- 1 and 490 +/- 20 muM
REFERENCE   6  (bases 1 to 577)
  AUTHORS   Bruneel,A., Labas,V., Mailloux,A., Sharma,S., Royer,N., Vinh,J.,
            Pernet,P., Vaubourdolle,M. and Baudin,B.
  TITLE     Proteomics of human umbilical vein endothelial cells applied to
            etoposide-induced apoptosis
  JOURNAL   Proteomics 5 (15), 3876-3884 (2005)
   PUBMED   16130169
REFERENCE   7  (bases 1 to 577)
  AUTHORS   Ek,P., Pettersson,G., Ek,B., Gong,F., Li,J.P. and Zetterqvist,O.
  TITLE     Identification and characterization of a mammalian 14-kDa
            phosphohistidine phosphatase
  JOURNAL   Eur J Biochem 269 (20), 5016-5023 (2002)
   PUBMED   12383260
  REMARK    GeneRIF: Northern blot analysis indicated that the human
            phosphohistidine phosphatase mRNA was present preferentially in
            heart and skeletal muscle.
REFERENCE   8  (bases 1 to 577)
  AUTHORS   Lai,C.H., Chiu,J.Y. and Lin,W.
  TITLE     Identification of the human crooked neck gene by comparative gene
            identification
  JOURNAL   Biochim Biophys Acta 1517 (3), 449-454 (2001)
   PUBMED   11342225
REFERENCE   9  (bases 1 to 577)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
REFERENCE   10 (bases 1 to 577)
  AUTHORS   Lanfranchi,G., Muraro,T., Caldara,F., Pacchioni,B., Pallavicini,A.,
            Pandolfo,D., Toppo,S., Trevisan,S., Scarso,S. and Valle,G.
  TITLE     Identification of 4370 expressed sequence tags from a
            3'-end-specific cDNA library of human skeletal muscle by DNA
            sequencing and filter hybridization
  JOURNAL   Genome Res 6 (1), 35-42 (1996)
   PUBMED   8681137
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL355987.31.
            
            On Jul 19, 2019 this sequence version replaced NM_014172.5.
            
            Summary: This gene encodes an enzyme that catalyzes the reversible
            dephosphorylation of histidine residues in proteins. It may be
            involved in the dephosphorylation of G-beta and ATP citrate lyase
            and in negatively regulating CD4 T lymphocytes by dephosphorylation
            and inhibition of KCa3.1 channels. Alternative splicing results in
            multiple transcript variants. [provided by RefSeq, Dec 2013].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CD388659.1, BP296211.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000247665.12/ ENSP00000247665.10
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-182               AL355987.31        127894-128075
            183-307             AL355987.31        128498-128622
            308-577             AL355987.31        129240-129509
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     gene            1..577
                     /gene="PHPT1"
                     /gene_synonym="CGI-202; HEL-S-132P; HSPC141; PHP; PHP14"
                     /note="phosphohistidine phosphatase 1"
                     /db_xref="GeneID:29085"
                     /db_xref="HGNC:HGNC:30033"
                     /db_xref="MIM:610167"
     exon            1..182
                     /gene="PHPT1"
                     /gene_synonym="CGI-202; HEL-S-132P; HSPC141; PHP; PHP14"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..400
                     /gene="PHPT1"
                     /gene_synonym="CGI-202; HEL-S-132P; HSPC141; PHP; PHP14"
                     /EC_number="3.9.1.3"
                     /note="isoform 3 is encoded by transcript variant 3; 14
                     kDa phosphohistidine phosphatase; sex-regulated protein
                     janus-a; protein janus-A homolog; epididymis secretory
                     sperm binding protein Li 132P; protein histidine
                     phosphatase"
                     /codon_start=1
                     /product="14 kDa phosphohistidine phosphatase isoform 3"
                     /protein_id="NP_054891.2"
                     /db_xref="CCDS:CCDS7009.1"
                     /db_xref="GeneID:29085"
                     /db_xref="HGNC:HGNC:30033"
                     /db_xref="MIM:610167"
                     /translation="MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIV
                     RGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQH
                     AISTEKIKAKYPDYEVTWANDGY"
     exon            183..307
                     /gene="PHPT1"
                     /gene_synonym="CGI-202; HEL-S-132P; HSPC141; PHP; PHP14"
                     /inference="alignment:Splign:2.1.0"
     exon            308..577
                     /gene="PHPT1"
                     /gene_synonym="CGI-202; HEL-S-132P; HSPC141; PHP; PHP14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      558..563
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHPT1"
                     /gene_synonym="CGI-202; HEL-S-132P; HSPC141; PHP; PHP14"
                     /note="hexamer: ATTAAA"
     polyA_site      577
                     /gene="PHPT1"
                     /gene_synonym="CGI-202; HEL-S-132P; HSPC141; PHP; PHP14"
                     /note="major polyA site"
ORIGIN      
        1 aggggactcc gggaggagga acatggcggt ggcggacctc gctctcattc ctgatgtgga
       61 catcgactcc gacggcgtct tcaagtatgt gctgatccga gtccactcgg ctccccgctc
      121 cggggctccg gctgcagaga gcaaggagat cgtgcgcggc tacaagtggg ctgagtacca
      181 tgcggacatc tacgacaaag tgtcgggcga catgcagaag caaggctgcg actgtgagtg
      241 tctgggcggc gggcgcatct cccaccagag tcaggacaag aagattcacg tgtacggcta
      301 ttccatggcc tatggtcctg cccagcacgc catttcaact gagaaaatca aagccaagta
      361 ccccgactac gaggtcacct gggctaacga cggctactga gcactcccag cccggggcct
      421 gctgcctcca gcagccactt cagagccccc gcctttgcct gcactcctct tgcagggctg
      481 gccctgcctg ctcctgcggc agcctctggt gacgtgctgt ccaccaggcc ttggagacag
      541 gctagcctgg ccacagaatt aaacgtgttg ccacacc
//
