LOCUS       NM_001199290            4183 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens PHOSPHO2-KLHL23 readthrough (PHOSPHO2-KLHL23),
            transcript variant 1, mRNA.
ACCESSION   NM_001199290
VERSION     NM_001199290.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Taipale,M., Tucker,G., Peng,J., Krykbaeva,I., Lin,Z.Y., Larsen,B.,
            Choi,H., Berger,B., Gingras,A.C. and Lindquist,S.
  TITLE     A quantitative chaperone interaction network reveals the
            architecture of cellular protein homeostasis pathways
  JOURNAL   Cell 158 (2), 434-448 (2014)
   PUBMED   25036637
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Prakash,T., Sharma,V.K., Adati,N., Ozawa,R., Kumar,N., Nishida,Y.,
            Fujikake,T., Takeda,T. and Taylor,T.D.
  TITLE     Expression of conjoined genes: another mechanism for gene
            regulation in eukaryotes
  JOURNAL   PLoS One 5 (10), e13284 (2010)
   PUBMED   20967262
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC016772.9 and AC009967.8.
            
            On Jun 2, 2019 this sequence version replaced NM_001199290.2.
            
            Summary: This locus represents naturally occurring read-through
            transcription between the neighboring PHOSPHO2 (phosphatase, orphan
            2) and KLHL23 (kelch-like 23) genes on chromosome 2. The
            read-through transcript includes only non-coding PHOSPHO2 exons,
            and thus encodes the KLHL23 protein. [provided by RefSeq, Dec
            2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the protein.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.146693.1,
                                           SRR1803617.236447.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2145240
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            readthrough transcript :: includes exons from GeneID 151230, 493911
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-154               AC016772.9         7398-7551           c
            155-187             AC016772.9         6773-6805           c
            188-358             AC016772.9         4489-4659           c
            359-1573            AC009967.8         33194-34408
            1574-1726           AC009967.8         39566-39718
            1727-4183           AC009967.8         47603-50059
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..4183
                     /gene="PHOSPHO2-KLHL23"
                     /note="PHOSPHO2-KLHL23 readthrough"
                     /db_xref="GeneID:100526832"
     exon            1..154
                     /gene="PHOSPHO2-KLHL23"
                     /inference="alignment:Splign:2.1.0"
     exon            155..187
                     /gene="PHOSPHO2-KLHL23"
                     /inference="alignment:Splign:2.1.0"
     exon            188..358
                     /gene="PHOSPHO2-KLHL23"
                     /inference="alignment:Splign:2.1.0"
     exon            359..1573
                     /gene="PHOSPHO2-KLHL23"
                     /inference="alignment:Splign:2.1.0"
     CDS             361..2037
                     /gene="PHOSPHO2-KLHL23"
                     /codon_start=1
                     /product="kelch-like protein 23"
                     /protein_id="NP_001186219.1"
                     /db_xref="GeneID:100526832"
                     /translation="MALKGQEDYIYLFKDSTHPVDFLDAFRTFYLDGLFTDITLQCPS
                     GIIFHCHRAVLAACSNYFKAMFTADMKEKFKNKIKLSGIHHDILEGLVNYAYTSQIEI
                     TKRNVQSLLEAADLLQFLSVKKACERFLVRHLDIDNCIGMHSFAEFHVCPELEKESRR
                     ILCSKFKEVWQQEEFLEISLEKFLFILSRKNLSVWKEEAIIEPVIKWTAHDVENRIEC
                     LYNLLSYINIDIDPVYLKTALGLQRSCLLTENKIRSLIYNALNPMHKEISQRSTATMY
                     IIGGYYWHPLSEVHIWDPLTNVWIQGAEIPDYTRESYGVTCLGPNIYVTGGYRTDNIE
                     ALDTVWIYNSESDEWTEGLPMLNARYYHCAVTLGGCVYALGGYRKGAPAEEAEFYDPL
                     KEKWIPIANMIKGVGNATACVLHDVIYVIGGHCGYRGSCTYDKVQSYNSDINEWSLIT
                     SSPHPEYGLCSVPFENKLYLVGGQTTITECYDPEQNEWREIAPMMERRMECGAVIMNG
                     CIYVTGGYSYSKGTYLQSIEKYDPDLNKWEIVGNLPSAMRSHGCVCVYNV"
     exon            1574..1726
                     /gene="PHOSPHO2-KLHL23"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..4183
                     /gene="PHOSPHO2-KLHL23"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4164..4169
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHOSPHO2-KLHL23"
                     /note="hexamer: AATAAA"
     polyA_site      4183
                     /gene="PHOSPHO2-KLHL23"
                     /note="major polyA site"
ORIGIN      
        1 gcagttggcg gtcgggctag agaagagagg cgcctgcgct tgcgagctgg gcttgtgagt
       61 ggggctgccg agagggcagg cgtggggcga ggccaaagga ctgaacccgc aggagcgtca
      121 cgggcgccgg ggcggctgcc gacggcggga ctgggttttc tatcagatgt tccacgtaat
      181 aatgctggag ttaagaagtt tccattattt tgctccaaac cagaagactc tgttccctgt
      241 atatagaata ggagtaatat ttgaaaacaa ctggctgatg tttaaaactg aagattgtca
      301 tgattgttta tcctaatccc aatgctgaag taagattgtc ttggaaatac taagttggcc
      361 atggctctaa aaggacaaga agattatatt tatcttttca aggattcaac acatccagtg
      421 gattttctgg atgcattcag aacattttac ttggatggat tatttactga tattactctt
      481 cagtgtcctt caggcataat tttccattgt caccgagccg ttttagctgc ttgcagcaat
      541 tattttaagg caatgttcac agctgacatg aaagaaaaat ttaaaaataa aataaaactc
      601 tctggcatcc accatgatat tctggaaggc cttgtaaatt atgcatacac ttcccaaatt
      661 gaaataacta aaagaaatgt tcaaagcctg cttgaggcag cggatctgct acagttcctt
      721 tcagtaaaga aggcttgtga gcggtttttg gtaaggcact tggatattga taattgtatt
      781 ggaatgcact cctttgcaga atttcatgtg tgtccagaac tagagaagga atctcgaaga
      841 attctatgtt caaagtttaa ggaagtgtgg caacaagaag aatttctgga aatcagcctt
      901 gaaaagtttc tctttatctt gtccagaaag aatctcagtg tttggaaaga agaagctatc
      961 atagagccag ttattaagtg gactgctcat gatgtagaaa atcgaattga atgcctctat
     1021 aatctactga gctatatcaa cattgatata gatccagtgt acttaaaaac agccttaggc
     1081 cttcaaagaa gctgcctgct caccgaaaat aagatccgct ccctaatata caatgccttg
     1141 aatcccatgc ataaagagat ttcccagagg tccacagcca caatgtatat aattggaggc
     1201 tattactggc atcctttatc agaggttcac atatgggatc ctttgacaaa tgtttggatt
     1261 cagggagcag aaataccaga ttataccagg gagagctatg gtgttacatg tttaggaccc
     1321 aacatttatg taactggggg ctacaggacg gataacatag aagctcttga cacagtgtgg
     1381 atctataaca gtgaaagtga tgaatggaca gaaggtttgc caatgctcaa tgccaggtat
     1441 taccactgtg cagtcacctt gggtggctgt gtctatgctt taggtggtta cagaaaaggg
     1501 gctccagcag aagaggctga gttctatgat cctttaaaag agaaatggat tcctattgca
     1561 aacatgatta aaggtgtggg aaatgctact gcctgtgtct tacatgatgt tatctacgtc
     1621 attggtggcc actgtggcta cagaggaagc tgcacctatg acaaagttca gagctacaat
     1681 tccgatatca acgaatggag cctcatcacc tccagtccac atccagaata tggattgtgc
     1741 tcagttccgt ttgaaaataa gctctatcta gtcggcggac aaactacaat cacagaatgc
     1801 tatgaccctg aacaaaatga atggagagag atagctccca tgatggaaag gaggatggag
     1861 tgcggtgccg tcatcatgaa tggatgtatt tatgtcactg gaggatactc ctactcaaag
     1921 ggaacgtatc ttcagagcat tgagaaatat gatccagatc ttaataagtg ggaaatagtg
     1981 ggtaatcttc ccagtgccat gcggtctcat gggtgtgttt gtgtgtataa tgtctaattg
     2041 aatctgcaga aatgaccaag caatcacttt tttggagtat agttttataa aaaaagaatg
     2101 cagggtttga agttccttac ctgataattg tgtctggcac atgatagggg atcagtaaat
     2161 tgtaattcct aaccctactg tactcccaaa catggtgatt catggtcaag aaaaatctta
     2221 tatatatata tatatacaca cacacatata tgtgttcata tatatgtata catatatatg
     2281 tgtatatata cgtatgtata catatatgtg tatatatacg tatgtatgta tacatatgtg
     2341 tatatatagt atgtatgtat acatgtatgt gtatatatac gtatgtatgt atacatatat
     2401 gtgtatacat atatatgtgt gtatatatat acacatatat acgtatatat gtgtatatat
     2461 atacacagtt gaatcagtgg gattaatacc tataatctct ggttttcaaa ggtaatatag
     2521 aatatttgac acttggtaaa aggtgaacta cctttgtagt gaatcttttc ctcttggtag
     2581 catcaacact ggggataaat cagaaccatt ctgtggaatg aaatgtttct caagagccta
     2641 taatatagta gatagtgcat attaagatgt ctggctgggc atggtggctc atgcctgtaa
     2701 tcccagcact ttgggaggct gaggcgggag gatcacttga gcctagaagt tggagactaa
     2761 cctggcgaga ccctgtctca aaaaaaaaaa aaaatcaatt tagctagcag gaatttgaca
     2821 tttaaagagg gaatcttctc tacctctaca aaatcaaccc ttaacacttt tttcaccttc
     2881 agaattattt agaatgtgga tttactttgt ctagtttttt cctttatagt gtaatttatt
     2941 gttactctct atttactgac taccagagat atacaaaata ctgtgcaaaa gattgttact
     3001 gttcaaagag cttacaaact aaatttaaaa gaatagcaag tctgaaaaat agaagatagt
     3061 caaatatttg tttatgctaa gaactgtttt taattttcag ctttgatgta aattgctagc
     3121 ttagttgtct ttagttcaag catgttagga aatcctcttt attcttaaat atagatacat
     3181 gtctgcatat atgattgaat tttagagcca gaaagcatgt agagcagttg tgattagtag
     3241 aaaatacact gacaagaaga tatttgcatt tatttcccaa agctgccagt aactagattt
     3301 tgtgacttaa gtcatttaac ctctccttga ttagagcttc ctgagccatg cacactggca
     3361 ctgcaaatca cagacaggcc tagatacaga tcccctcacc ccttgctaca tccaaaggtg
     3421 gcctgggtgg ctggagcctc tgggccagaa ccacctttgt ccattttcct cagtgtgcct
     3481 acagcatcgc tttccatgtg tgccataact tgggaatagt tgggatgtgt tgcttaactc
     3541 ctttacacca ttagttatct gtgagaccat ctattccaca ctcttcattt tttaaaaaga
     3601 gacaataatt atttttaact cactgaagtt taaaactacc tagcatttct gattaaaaaa
     3661 aatttagaag gaaaatttta atgcatttat ttcagatggc gttttaaata gatacaaccc
     3721 tttggaaaca caatatggta ctacatagca agggctgtaa aaatgaccat atcctttgcc
     3781 ccataaattc tacttctata cttttaacct taaaaataat tcagaaaaaa gataaatgca
     3841 tgaagacatt tatgtctgca ttctttataa taacaaaaat tggggaaaaa aactccatgt
     3901 caagaaagtg ggataagaaa aatagtgaag tattatactg ccattaaaat aattatttgg
     3961 aagactgtag aaacgtggac atgtttgata tggtaagtaa aatgagcaca atacaaaata
     4021 gtatgcatac tatgattgtg actttgtaaa atctttatgc atgtggaagg taatctgcaa
     4081 aacaaattca ttgtgttagt gtagcgggat atggaatgac tttatttatt taaatctttt
     4141 ctttaatatt actttgtatt ttcaataaaa ataaatcact gta
//
