LOCUS       NM_015020               8317 bp    mRNA    linear   PRI 05-JUN-2024
DEFINITION  Homo sapiens PH domain and leucine rich repeat protein phosphatase
            2 (PHLPP2), transcript variant 1, mRNA.
ACCESSION   NM_015020 XM_041191
VERSION     NM_015020.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Etemad-Moghadam,S., Mohammadpour,H., Emami Razavi,A. and
            Alaeddini,M.
  TITLE     Pleckstrin Homology Domain Leucine-rich Repeat Protein Phosphatase
            Acts as a Tumor Suppressor in Oral Squamous Cell Carcinoma
  JOURNAL   Appl Immunohistochem Mol Morphol 32 (5), 249-253 (2024)
   PUBMED   38602289
  REMARK    GeneRIF: Pleckstrin Homology Domain Leucine-rich Repeat Protein
            Phosphatase Acts as a Tumor Suppressor in Oral Squamous Cell
            Carcinoma.
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Kang,D., Hwang,H.J., Baek,Y., Sung,J.Y., Kim,K., Park,H.J.,
            Ko,Y.G., Kim,Y.N. and Lee,J.S.
  TITLE     TRIM22 induces cellular senescence by targeting PHLPP2 in
            hepatocellular carcinoma
  JOURNAL   Cell Death Dis 15 (1), 26 (2024)
   PUBMED   38199981
  REMARK    GeneRIF: TRIM22 induces cellular senescence by targeting PHLPP2 in
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8317)
  AUTHORS   Sharma,M. and Dey,C.S.
  TITLE     PHLPP isoforms differentially regulate Akt isoforms and AS160
            affecting neuronal insulin signaling and insulin resistance via
            Scribble
  JOURNAL   Cell Commun Signal 20 (1), 179 (2022)
   PUBMED   36376971
  REMARK    GeneRIF: PHLPP isoforms differentially regulate Akt isoforms and
            AS160 affecting neuronal insulin signaling and insulin resistance
            via Scribble.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8317)
  AUTHORS   Iyer,D.N., Foo,D.C., Lo,O.S., Wan,T.M., Li,X., Sin,R.W., Pang,R.W.,
            Law,W.L. and Ng,L.
  TITLE     MiR-509-3p is oncogenic, targets the tumor suppressor PHLPP2, and
            functions as a novel tumor adjacent normal tissue based prognostic
            biomarker in colorectal cancer
  JOURNAL   BMC Cancer 22 (1), 351 (2022)
   PUBMED   35361144
  REMARK    GeneRIF: MiR-509-3p is oncogenic, targets the tumor suppressor
            PHLPP2, and functions as a novel tumor adjacent normal tissue based
            prognostic biomarker in colorectal cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8317)
  AUTHORS   Liu,N., Jiang,X., Guo,L., Zhang,C., Jiang,M., Sun,Z., Zhang,Y.,
            Mi,W., Li,J., Fu,Y., Wang,F., Zhang,L. and Zhang,Y.
  TITLE     Mutant p53 achieved Gain-of-Function by promoting tumor growth and
            immune escape through PHLPP2/AKT/PD-L1 pathway
  JOURNAL   Int J Biol Sci 18 (6), 2419-2438 (2022)
   PUBMED   35414774
  REMARK    GeneRIF: Mutant p53 achieved Gain-of-Function by promoting tumor
            growth and immune escape through PHLPP2/AKT/PD-L1 pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8317)
  AUTHORS   Hirano,I., Nakamura,S., Yokota,D., Ono,T., Shigeno,K., Fujisawa,S.,
            Shinjo,K. and Ohnishi,K.
  TITLE     Depletion of Pleckstrin homology domain leucine-rich repeat protein
            phosphatases 1 and 2 by Bcr-Abl promotes chronic myelogenous
            leukemia cell proliferation through continuous phosphorylation of
            Akt isoforms
  JOURNAL   J Biol Chem 284 (33), 22155-22165 (2009)
   PUBMED   19261608
  REMARK    GeneRIF: Bcr-Abl represses the expression of PHLPP1 and PHLPP2 and
            continuously activates Akt1, -2, and -3 via phosphorylation on
            Ser-473, resulting in the proliferation of CML cells
REFERENCE   7  (bases 1 to 8317)
  AUTHORS   Gao,T., Brognard,J. and Newton,A.C.
  TITLE     The phosphatase PHLPP controls the cellular levels of protein
            kinase C
  JOURNAL   J Biol Chem 283 (10), 6300-6311 (2008)
   PUBMED   18162466
REFERENCE   8  (bases 1 to 8317)
  AUTHORS   Brognard,J., Sierecki,E., Gao,T. and Newton,A.C.
  TITLE     PHLPP and a second isoform, PHLPP2, differentially attenuate the
            amplitude of Akt signaling by regulating distinct Akt isoforms
  JOURNAL   Mol Cell 25 (6), 917-931 (2007)
   PUBMED   17386267
REFERENCE   9  (bases 1 to 8317)
  AUTHORS   Martin,J., Han,C., Gordon,L.A., Terry,A., Prabhakar,S., She,X.,
            Xie,G., Hellsten,U., Chan,Y.M., Altherr,M., Couronne,O., Aerts,A.,
            Bajorek,E., Black,S., Blumer,H., Branscomb,E., Brown,N.C.,
            Bruno,W.J., Buckingham,J.M., Callen,D.F., Campbell,C.S.,
            Campbell,M.L., Campbell,E.W., Caoile,C., Challacombe,J.F.,
            Chasteen,L.A., Chertkov,O., Chi,H.C., Christensen,M., Clark,L.M.,
            Cohn,J.D., Denys,M., Detter,J.C., Dickson,M.,
            Dimitrijevic-Bussod,M., Escobar,J., Fawcett,J.J., Flowers,D.,
            Fotopulos,D., Glavina,T., Gomez,M., Gonzales,E., Goodstein,D.,
            Goodwin,L.A., Grady,D.L., Grigoriev,I., Groza,M., Hammon,N.,
            Hawkins,T., Haydu,L., Hildebrand,C.E., Huang,W., Israni,S.,
            Jett,J., Jewett,P.B., Kadner,K., Kimball,H., Kobayashi,A.,
            Krawczyk,M.C., Leyba,T., Longmire,J.L., Lopez,F., Lou,Y., Lowry,S.,
            Ludeman,T., Manohar,C.F., Mark,G.A., McMurray,K.L., Meincke,L.J.,
            Morgan,J., Moyzis,R.K., Mundt,M.O., Munk,A.C., Nandkeshwar,R.D.,
            Pitluck,S., Pollard,M., Predki,P., Parson-Quintana,B., Ramirez,L.,
            Rash,S., Retterer,J., Ricke,D.O., Robinson,D.L., Rodriguez,A.,
            Salamov,A., Saunders,E.H., Scott,D., Shough,T., Stallings,R.L.,
            Stalvey,M., Sutherland,R.D., Tapia,R., Tesmer,J.G., Thayer,N.,
            Thompson,L.S., Tice,H., Torney,D.C., Tran-Gyamfi,M., Tsai,M.,
            Ulanovsky,L.E., Ustaszewska,A., Vo,N., White,P.S., Williams,A.L.,
            Wills,P.L., Wu,J.R., Wu,K., Yang,J., Dejong,P., Bruce,D.,
            Doggett,N.A., Deaven,L., Schmutz,J., Grimwood,J., Richardson,P.,
            Rokhsar,D.S., Eichler,E.E., Gilna,P., Lucas,S.M., Myers,R.M.,
            Rubin,E.M. and Pennacchio,L.A.
  TITLE     The sequence and analysis of duplication-rich human chromosome 16
  JOURNAL   Nature 432 (7020), 988-994 (2004)
   PUBMED   15616553
REFERENCE   10 (bases 1 to 8317)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX647823.1, AC009097.9 and BC129927.1.
            
            On Jan 10, 2014 this sequence version replaced NM_015020.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647823.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000568954.5/ ENSP00000457991.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-234               BX647823.1         2-235
            235-235             AC009097.9         185248-185248       c
            236-817             BX647823.1         237-818
            818-4313            BC129927.1         445-3940
            4314-8317           AC009097.9         105705-109708       c
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.2"
     gene            1..8317
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /note="PH domain and leucine rich repeat protein
                     phosphatase 2"
                     /db_xref="GeneID:23035"
                     /db_xref="HGNC:HGNC:29149"
                     /db_xref="MIM:611066"
     exon            1..373
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            374..663
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     CDS             380..4351
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /EC_number="3.1.3.16"
                     /note="isoform 1 is encoded by transcript variant 1; PH
                     domain leucine-rich repeat-containing protein phosphatase
                     2; protein phosphatase, Mg2+/Mn2+ dependent 3B"
                     /codon_start=1
                     /product="PH domain leucine-rich repeat-containing protein
                     phosphatase 2 isoform 1"
                     /protein_id="NP_055835.2"
                     /db_xref="CCDS:CCDS32479.1"
                     /db_xref="GeneID:23035"
                     /db_xref="HGNC:HGNC:29149"
                     /db_xref="MIM:611066"
                     /translation="MKRNGSRNCLNRRSRFGSRERDWLREDVKRGCVYLYGADTTTAT
                     TTTTTSSSSSSSSSSSDLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTER
                     PLQIVYDYLSRLGFDDPVRIQEEATNPDLGCMIRFYGEKPCHMDRLDRILLSGIYNVR
                     KGKTQLHKWAERLVVLCGTCLIVSSVKDCQTGKMHILPLVGGKIEEVKRRQYSLAFSS
                     AGAQAQTYHVSFETLAEYQRWQRQASKVVSQRISTVDLSCYSLEEVPEHLFYSQDITY
                     LNLRHNFMQLERPGGLDTLYKFSQLKGLNLSHNKLGLFPILLCEISTLTELNLSCNGF
                     HDLPSQIGNLLNLQTLCLDGNFLTTLPEELGNLQQLSSLGISFNNFSQIPEVYEKLTM
                     LDRVVMAGNCLEVLNLGVLNRMNHIKHVDLRMNHLKTMVIENLEGNKHITHVDLRDNR
                     LTDLDLSSLCSLEQLHCGRNQLRELTLSGFSLRTLYASSNRLTAVNVYPVPSLLTFLD
                     LSRNLLECVPDWACEAKKIEVLDVSYNLLTEVPVRILSSLSLRKLMLGHNHVQNLPTL
                     VEHIPLEVLDLQHNALTRLPDTLFSKALNLRYLNASANSLESLPSACTGEESLSMLQL
                     LYLTNNLLTDQCIPVLVGHLHLRILHLANNQLQTFPASKLNKLEQLEELNLSGNKLKT
                     IPTTIANCKRLHTLVAHSNNISIFPEILQLPQIQFVDLSCNDLTEILIPEALPATLQD
                     LDLTGNTNLVLEHKTLDIFSHITTLKIDQKPLPTTDSTVTSTFWSHGLAEMAGQRNKL
                     CVSALAMDSFAEGVGAVYGMFDGDRNEELPRLLQCTMADVLLEEVQQSTNDTVFMANT
                     FLVSHRKLGMAGQKLGSSALLCYIRPDTADPASSFSLTVANVGTCQAVLCRGGKPVPL
                     SKVFSLEQDPEEAQRVKDQKAIITEDNKVNGVTCCTRMLGCTYLYPWILPKPHISSTP
                     LTIQDELLILGNKALWEHLSYTEAVNAVRHVQDPLAAAKKLCTLAQSYGCQDNVGAMV
                     VYLNIGEEGCTCEMNGLTLPGPVGFASTTTIKDAPKPATPSSSSGIASEFSSEMSTSE
                     VSSEVGSTASDEHNAGGLDTALLPRPERRCSLHPTPTSGLFQRQPSSATFSSNQSDNG
                     LDSDDDQPVEGVITNGSKVEVEVDIHCCRGRDLENSPPLIESSPTLCSEEHARGSCFG
                     IRRQNSVNSGMLLPMSKDRMELQKSPSTSCLYGKKLSNGSIVPLEDSLNLIEVATEVP
                     KRKTGYFAAPTQMEPEDQFVVPHDLEEEVKEQMKQHQDSRLEPEPHEEDRTEPPEEFD
                     TAL"
     exon            664..797
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            798..988
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1114
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1269
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1416
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1647
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1850
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1911
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2007
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2163
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2364
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2527
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2658
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..2769
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2770..2964
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2965..3196
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3197..8317
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8281..8286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /note="hexamer: AATAAA"
     polyA_site      8317
                     /gene="PHLPP2"
                     /gene_synonym="PHLPPL; PPM3B"
                     /note="major polyA site"
ORIGIN      
        1 gcacaggggt atagatctaa agaagggcag aggggagatc tggaaacagg ctggctgact
       61 ccaaatttgg cccattggca acaaaactac tatcactgcg agaacgaaaa gaaaaacaaa
      121 acaaagaaaa gaaaagaagt taacttttaa aagttttttt ctgaatcagc cttgcaaata
      181 aatcataaac cgggtcacag gccatattac tgcttgaagc agagcaccga attctgataa
      241 ttttgctcca ggaggaggca agggaattaa aacatggctg ggaggtgagc ggacgttttg
      301 tacgaggttg ggttgaattt gcttaagaag gtcgcacgca tctctgataa ggaagagaaa
      361 aggccttgcc aagagaaata tgaaacgcaa tgggagcaga aattgtttga ataggagaag
      421 taggtttggt tctcgagaaa gagactggct aagagaagat gtaaagagag gctgtgttta
      481 cctttatgga gcagacacta ccactgccac tacaaccacc accacctcct cttcctcttc
      541 ctcctcctcc tcttcctctg acttacatct cgtcctttgc actgtagaga caccagcatc
      601 agaaatatgt gctggagagg gaagagaaag tctttattta cagcttcatg gagacctggt
      661 caggagactg gaacctactg aacgacctct tcagatcgtt tatgattact tatccaggct
      721 gggatttgat gatcctgtgc gcatacagga ggaggctaca aatcctgacc tcggctgtat
      781 gattcgattt tatggtgaaa aaccatgcca catggatcgt ttggatcgaa tcctattgtc
      841 tggcatctat aatgtacgca agggaaagac ccagctgcat aagtgggctg agcgcctagt
      901 tgtcctctgt ggtacctgcc ttatcgtttc ctcagtgaag gattgtcaaa ctggaaagat
      961 gcacattttg cctctggttg gtggaaagat agaagaagtg aagcgacggc aatactccct
     1021 tgctttcagc tcagcaggag cccaagctca gacctatcat gtcagcttcg agactttggc
     1081 cgagtaccag cgatggcaac ggcaagcatc caaggtggtg tcccagcgaa tcagtaccgt
     1141 ggatctctcg tgttacagcc tcgaggaggt tcctgagcat ctcttctata gtcaagatat
     1201 tacctacctc aacttgcgac acaacttcat gcagttagaa agacccggag gcctcgatac
     1261 actctacaaa ttttctcaac tgaagggcct gaacttgtcc cataataaac ttgggttgtt
     1321 tcctatattg ttatgcgaga tctctaccct gactgagctc aacctttcct gtaatggatt
     1381 tcatgaccta ccaagtcaaa ttggcaatct gctaaatctt caaaccctct gtcttgatgg
     1441 caactttctg actactttac ctgaagaatt gggaaatcta caacagcttt cctccttggg
     1501 aatttccttc aacaacttta gtcaaattcc tgaggtttat gagaaactca ctatgttaga
     1561 tagagtggtt atggcaggaa attgcctgga agtcctgaac ttaggggtgc tgaataggat
     1621 gaaccatatc aagcatgtgg atttaaggat gaaccatttg aaaaccatgg ttattgaaaa
     1681 tctggaggga aataaacaca tcacccacgt ggatctgcgg gacaaccgac tgactgactt
     1741 ggatcttagc tccttatgca gcttggaaca gctgcactgt gggcggaatc agctgaggga
     1801 gctaacactc agtggctttt cccttcggac cctctatgcc agttccaaca ggctgacagc
     1861 agtgaacgtc tatccagtac ccagcctgct cactttcttg gatctctccc gaaacctgct
     1921 agagtgtgtc cctgactggg cctgtgaagc aaagaagata gaagtattag atgtgagcta
     1981 taatcttctc acagaggttc ccgtgagaat tctgagtagc ttgagtctta gaaaactgat
     2041 gctgggacac aatcatgtgc aaaaccttcc aacactggta gagcacatcc ccctcgaggt
     2101 gctggatctt cagcataatg cactcacgag gctgccagac accctcttct ccaaggcctt
     2161 aaatctcaga tacttgaatg catctgcaaa tagtctggag tctttaccat ccgcctgcac
     2221 tggagaggag agtttgagta tgctgcagct gctttatctg accaacaatc tcctgacgga
     2281 tcagtgcata cctgtcctgg tagggcacct gcacctgcga atcttgcacc ttgcaaacaa
     2341 tcagttacag acctttcctg caagcaaact aaataaattg gagcaattgg aggaactgaa
     2401 cctaagtggc aacaagctta aaaccattcc cacaaccata gcaaactgta aaaggctgca
     2461 cacccttgtt gcacactcca acaacatcag cattttccca gaaatactgc agttgcctca
     2521 gatccagttt gtagacctaa gttgcaacga cttgacagaa atcctgattc cagaggcttt
     2581 gcctgctaca ttacaagacc ttgacctgac tggaaataca aatctggttc tggaacacaa
     2641 gacactggac atatttagcc atatcacaac cctgaaaatt gatcagaaac ctttgccaac
     2701 cacagattct acagttacgt caaccttctg gagccatgga ctggctgaga tggcagggca
     2761 gagaaataag ctgtgtgtct cagcacttgc tatggatagc tttgcagagg gggtgggagc
     2821 tgtgtatggc atgtttgatg gagaccgaaa tgaggagctc ccgcgcctgc tgcagtgtac
     2881 gatggcagat gtgcttttag aagaggtaca gcagtcaact aatgacacag ttttcatggc
     2941 taacaccttc ttggtatctc acaggaaatt aggaatggct ggccagaagt tgggctcctc
     3001 cgctctcctg tgctacatcc gccctgacac tgccgatcca gcaagtagct ttagcttgac
     3061 tgtagccaat gttggcacgt gccaagcagt cctgtgccga ggtgggaagc cagtgcccct
     3121 ctctaaagtc ttcagcctgg agcaggaccc agaggaggct caaagggtga aggaccaaaa
     3181 agccatcatc acagaggaca acaaagtgaa tggggtaacc tgctgtaccc ggatgctggg
     3241 ctgtacatac ctctaccctt ggatcctccc caagccccac atatcttcca ctccgctgac
     3301 cattcaagat gagttgctga ttctgggaaa caaagcattg tgggaacact tgtcctacac
     3361 agaagctgtc aatgctgtac gtcacgtaca agacccatta gcagctgcta agaagctgtg
     3421 cacattagcg cagagctatg gctgtcagga caatgtaggg gcgatggtag tttatttgaa
     3481 tattggtgag gaaggctgca cttgtgaaat gaatgggctc accctcccag gtcctgtggg
     3541 atttgcttca accaccacta tcaaggatgc ccctaagcca gccactccat cctctagcag
     3601 tgggattgcc tctgagttca gcagtgagat gtccacctca gaggtgagca gtgaagtggg
     3661 gtccactgct tctgatgagc ataatgctgg gggcctggac actgccttgc ttccgaggcc
     3721 agagcggcgc tgcagcctcc acccaacacc cacctctggg ctgtttcagc gccagccttc
     3781 ttctgctacc ttctccagta accagtctga caacggcctg gacagtgatg atgaccagcc
     3841 cgttgagggg gtcataacca atggcagcaa ggtagaggtg gaagtagaca tccactgctg
     3901 cagggggagg gatctggaga actcaccccc tctcatagag agttctccta ccctgtgttc
     3961 tgaggaacat gctagagggt cgtgttttgg gatccgaaga cagaacagtg tgaatagtgg
     4021 catgctcctg ccaatgagca aggacaggat ggagttacag aagtctccct ccacctcctg
     4081 cctctatggg aagaaactct ccaatggctc tattgtgccc ctagaggaca gcctgaacct
     4141 cattgaagtg gccacagaag tgcccaagag gaaaactggc tattttgctg cccccactca
     4201 gatggaacca gaggaccagt ttgttgtgcc tcatgacctg gaagaagaag tgaaggaaca
     4261 aatgaaacag caccaggaca gccggctcga gcctgagccc catgaagagg atcggaccga
     4321 gcccccggag gagttcgaca cagcactatg actgccccac tgggcacagt gtgggaggag
     4381 gctgtgcagg gttggggtag ggacttgcta gaggcattct gcctctacat ttctttttgt
     4441 ttgttcgttt tttttttgtt tgtttgtttt gagacggagt cttgctcagt cgctcaggct
     4501 ggagtgcaat ggtggggtct cgggtcactg cagcctctgt ccctgggttc aagccattct
     4561 cctgtctcag cctcccgagt agctgggatt acaggcacct gccattatgc ccggctaagt
     4621 tttatttttt ttttttagag acggggtttc accatgttgg ccaggctggt gttgaactct
     4681 tgacctcagg tgatccaccc tcctctgcct cccaaagtgc tgtgattaca ggcctcagcc
     4741 accatgccca gccctgcgtc tacatttcta aaccatagct gtgtggggtt gaactcggag
     4801 ccaaaaagtg tgagagccat caggggctgg ctctggataa actggtagcc actatcagtg
     4861 ttaagtttca catttaacct gcattggaat tcccaggggt actgggaaga aagcagctgt
     4921 tctgtatcag tcctaccacc tgccattaac cctttctctc ctaggatcat tttgagaatt
     4981 tgcctgcctg ggcaggaaag ggactatttc tgtggaggaa aaaagtgaag attgattctc
     5041 tttactagtt gctgctgatg gatctctgtg acagagaaat caccttatct cagactaatg
     5101 gggtgtgatg tgactagtca catggctttt cattcttctc tacgagaata cagcctatca
     5161 aaatgatgtc tgttggaaat gtagaaccaa tcaaacagat aatttatgta tgtaatgtaa
     5221 tgagagcact tttcattgac tgtgaacttt ttatttttga atctgcactc gagccaatct
     5281 tcttagaggc agcccggcac cttcatccat aggcagagag agaactgggt gttggagact
     5341 tattcgaggg tataggaagg gccctgtgaa gttgatttaa cttttggatg tcagactgtg
     5401 aaagctcctg agaaacttgg ggtaatagga tcttcttttg gggatgaaaa tggggaaggc
     5461 gtgaggacct agactacttc tccctagatc agaaaaagag aattacccct tgacaaatat
     5521 gatacctgct aggtatttcc cagggaaatt tagggattgg cgtctttccc tagcatgtgg
     5581 aggaattggc agacagcttc ctaagggcgg ggagcggggg cccaaggctg acactgcttg
     5641 catccacgtg accttaagtt atggcagatg actctgaaac ggactgaggc caatgagaac
     5701 agatggatgg agcactcagg ttagacttgt tccttctcct atgctggagg agagggatgg
     5761 ttctctagaa tgttggaggt gagttgagag ctcgcctctt gaatgttgaa cagtgtactc
     5821 ttctgaaaac tgcatattca ctttatgtgg tttcagaata ctgggctcaa tactaacata
     5881 agaaagacac ttcattgaga aattcttaag cttacagaaa acctatactc tttgcacatt
     5941 ccacataacc ctagcaaaat gcagtttctt catacttctg tcactttttc cattggaaga
     6001 tttgcttagg aaaattaatt cctatttatt cccacaaaat gttggcattg cttgatttta
     6061 cccaatgggg aatgtgcttt gaatttttgg aacactttta caattaaaaa taaagtaggg
     6121 ccatttttaa tttgtttcat cagaaactat gttaaagaga gggttaaata taaatatatt
     6181 ttcgtgtgta tttttgggaa gatttttgtt caaagcaata gtcaaaatca gatgacctgt
     6241 ccataataat tatgtgtctt catcttctca gaggccccat gctcatatgc acgtgtcatt
     6301 gggatacact cttgggggat ttggtacact ctaatggatg tctaatgttc aacccctcga
     6361 gaatctgaac ttgagtcccc agattgtcaa actactggtc agctattgag aattttagaa
     6421 ctcaggtctt tgatttgaag tagggaacat agtggctcac acagagttta ggtgctgtta
     6481 gaaagatggg aacaagagtg ttttgccacc ttatttttat atgggaaatt ttttttaatg
     6541 aagaaaaaat gaaaatgaaa taacaggatg atagtgatga gtgatgtaaa acatcttact
     6601 tagatggcag aaccttcggg ttgtagaata gtgatgtcta aaaattaaag ttattttggg
     6661 aatacaccac tttaatagta tagtcttata aaaattattc attatggtga acccttcttg
     6721 tctgctattc gtttcccaac ctacttattg gaaccacctc aaaacccagt tctgaaatga
     6781 ccttaccaaa agtaaatgta tttatttttt agtcagcaga actctgtaat tccaaatgtt
     6841 gttctgtgtg gtagaatttt ttttcaggaa ccattaggtt gtattgaaaa tctttgttat
     6901 aggcgatacc aaaactgatt attctttttt gcagtctgct ttaattcatg tccttctgca
     6961 gttgctctgt attaaaacag ggtaaaaagg ccatagccca ttatgaaaaa tataatacaa
     7021 aactctttga cctatgaggt aacttacaga cattgtgttt tctaaacagg ctgtcagtga
     7081 aagcccgtat ctgtctccag gtgaatgtaa tttacttccg agtactttac ccagaggatg
     7141 tgttccccag gtgggcagag tacagttgat ctctagcaca gacagagatt ctggcctctg
     7201 catattctca ggtctctgtg tgtacctccc attgagtaga gaagcttaag ataatttctg
     7261 agagaagaac actgctgatt gtgggagcag tttaggagtc catggaagaa agaaaaatac
     7321 atgtgtcttg gcagccatgg tgtatttttg tccaaatgga ttggaaggat atttgaatat
     7381 ttgaatgttg gtatgacata aagctgcagt gcactataga gtcaagtcat tgaattacca
     7441 ctcctgatac agggctttat tgtactactg tgaagtgtat gtgtgcaata cattggtgag
     7501 ttcatttact ggtgtacgga agagccagca ggagcagcgt ggtcattgct gggtgctatt
     7561 acagttgctt gtagtgagtg ctgttttcca ggagatggag ccagttgggt gtggcagatc
     7621 tactgaatat caaatgatgc tcttcttccc atgtagacct tcagcaaaag ccggtacttg
     7681 gaagccacag gctcaccttc tctatctatc caataattat taatgaagag acctccataa
     7741 gggagcagct ggctgttatc gataaatgta ccaattatta aataattagt ctccaagcca
     7801 ttcagtgatg tcttcagcat cactatagga ctgtctagtg tcacttttta cttccttctg
     7861 ggtggaggct ttccgactcc caatcatgaa ggcaagttaa tctttccagt tagtgacttt
     7921 tgccccatag ttggggtaag cacttcctag attgagaaaa agcagctaca gtcaatcctg
     7981 ctctgtttgc ctcatttggt gatcagtcag tcacacataa gttccttgta ttctaaattt
     8041 catgcacttc tcccagatgc tatagggttt tctctcactg ttgccagtgg atgtcatcca
     8101 gacagtgggc tcatatctta cggttttgtg caatcattgt cgtattgtag tcttaagact
     8161 cattatagtg tatttttgat atttttgaaa tgtgttaaat tttttaattc aataatatga
     8221 gccagagcat gttgcagcaa atctattgtt tgtaaaaata acaataacaa taataaataa
     8281 aataaagtgg aatctttttc atggctttgt tttaaaa
//
