LOCUS       NM_194449               6299 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens PH domain and leucine rich repeat protein phosphatase
            1 (PHLPP1), mRNA.
ACCESSION   NM_194449 XM_166290
VERSION     NM_194449.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Weaver,S.R., Peralta-Herrera,E., Torres,H.M., Jessen,E.,
            Bradley,E.W. and Westendorf,J.J.
  TITLE     Phlpp1 alters the murine chondrocyte phospho-proteome during
            endochondral bone formation
  JOURNAL   Bone 189, 117265 (2024)
   PUBMED   39349089
  REMARK    GeneRIF: Phlpp1 alters the murine chondrocyte phospho-proteome
            during endochondral bone formation.
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Etemad-Moghadam,S., Mohammadpour,H., Emami Razavi,A. and
            Alaeddini,M.
  TITLE     Pleckstrin Homology Domain Leucine-rich Repeat Protein Phosphatase
            Acts as a Tumor Suppressor in Oral Squamous Cell Carcinoma
  JOURNAL   Appl Immunohistochem Mol Morphol 32 (5), 249-253 (2024)
   PUBMED   38602289
  REMARK    GeneRIF: Pleckstrin Homology Domain Leucine-rich Repeat Protein
            Phosphatase Acts as a Tumor Suppressor in Oral Squamous Cell
            Carcinoma.
REFERENCE   3  (bases 1 to 6299)
  AUTHORS   Andreozzi,F., Procopio,C., Greco,A., Mannino,G.C., Miele,C.,
            Raciti,G.A., Iadicicco,C., Beguinot,F., Pontiroli,A.E.,
            Hribal,M.L., Folli,F. and Sesti,G.
  TITLE     Increased levels of the Akt-specific phosphatase PH domain
            leucine-rich repeat protein phosphatase (PHLPP)-1 in obese
            participants are associated with insulin resistance
  JOURNAL   Diabetologia 54 (7), 1879-1887 (2011)
   PUBMED   21461637
  REMARK    GeneRIF: Increased abundance of PHLPP-1, production of which is
            regulated by insulin, may represent a new molecular defect in
            insulin-resistant states such as obesity.
REFERENCE   4  (bases 1 to 6299)
  AUTHORS   Suljagic,M., Laurenti,L., Tarnani,M., Alam,M., Malek,S.N. and
            Efremov,D.G.
  TITLE     Reduced expression of the tumor suppressor PHLPP1 enhances the
            antiapoptotic B-cell receptor signal in chronic lymphocytic
            leukemia B-cells
  JOURNAL   Leukemia 24 (12), 2063-2071 (2010)
   PUBMED   20861921
  REMARK    GeneRIF: Reduced expression of the tumor suppressor PHLPP1 enhances
            the antiapoptotic B-cell receptor signal in chronic lymphocytic
            leukemia B-cells.
REFERENCE   5  (bases 1 to 6299)
  AUTHORS   Jackson,T.C., Verrier,J.D., Semple-Rowland,S., Kumar,A. and
            Foster,T.C.
  TITLE     PHLPP1 splice variants differentially regulate AKT and PKCalpha
            signaling in hippocampal neurons: characterization of PHLPP
            proteins in the adult hippocampus
  JOURNAL   J Neurochem 115 (4), 941-955 (2010)
   PUBMED   20819118
REFERENCE   6  (bases 1 to 6299)
  AUTHORS   Kanan,Y., Matsumoto,H., Song,H., Sokolov,M., Anderson,R.E. and
            Rajala,R.V.
  TITLE     Serine/threonine kinase akt activation regulates the activity of
            retinal serine/threonine phosphatases, PHLPP and PHLPPL
  JOURNAL   J Neurochem 113 (2), 477-488 (2010)
   PUBMED   20089132
REFERENCE   7  (bases 1 to 6299)
  AUTHORS   Liu,J., Weiss,H.L., Rychahou,P., Jackson,L.N., Evers,B.M. and
            Gao,T.
  TITLE     Loss of PHLPP expression in colon cancer: role in proliferation and
            tumorigenesis
  JOURNAL   Oncogene 28 (7), 994-1004 (2009)
   PUBMED   19079341
  REMARK    GeneRIF: Loss of PHLPP expression is associated with colon cancer.
REFERENCE   8  (bases 1 to 6299)
  AUTHORS   Brognard,J., Sierecki,E., Gao,T. and Newton,A.C.
  TITLE     PHLPP and a second isoform, PHLPP2, differentially attenuate the
            amplitude of Akt signaling by regulating distinct Akt isoforms
  JOURNAL   Mol Cell 25 (6), 917-931 (2007)
   PUBMED   17386267
  REMARK    GeneRIF: Mechanism to selectively terminate Akt-signaling pathways
            through the differential inactivation of specific Akt isoforms by
            specific PHLPP isoforms.
REFERENCE   9  (bases 1 to 6299)
  AUTHORS   Gao,T., Furnari,F. and Newton,A.C.
  TITLE     PHLPP: a phosphatase that directly dephosphorylates Akt, promotes
            apoptosis, and suppresses tumor growth
  JOURNAL   Mol Cell 18 (1), 13-24 (2005)
   PUBMED   15808505
REFERENCE   10 (bases 1 to 6299)
  AUTHORS   Shimizu,K., Okada,M., Takano,A. and Nagai,K.
  TITLE     SCOP, a novel gene product expressed in a circadian manner in rat
            suprachiasmatic nucleus
  JOURNAL   FEBS Lett 458 (3), 363-369 (1999)
   PUBMED   10570941
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022046.11, BC126277.1,
            AC015989.11 and AC027553.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_194449.3.
            
            Summary: This gene encodes a member of the serine/threonine
            phosphatase family. The encoded protein promotes apoptosis by
            dephosphorylating and inactivating the serine/threonine kinase Akt,
            and functions as a tumor suppressor in multiple types of cancer.
            Increased expression of this gene may also play a role in obesity
            and type 2 diabetes by interfering with Akt-mediated insulin
            signaling. [provided by RefSeq, Dec 2011].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to one that is more supported by available conservation
            data and publications. There are no publicly available human
            transcripts that include the extended region. However, the update
            is supported by homologous transcript data and is consistent with
            the full-length 190 kDa human isoform described in the literature.
            This 190 kDa product, known as PHLPP1beta, has been detected in
            several studies, including PMIDs 17386267, 19079341, 20089132,
            20819118 and 20861921.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014927.2, AB011178.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262719.10/ ENSP00000262719.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1563              AC022046.11        124777-126339
            1564-2263           BC126277.1         1-700
            2264-2264           AC015989.11        140867-140867
            2265-5649           BC126277.1         702-4086
            5650-6299           AC027553.6         51332-51981         c
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.33"
     gene            1..6299
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /note="PH domain and leucine rich repeat protein
                     phosphatase 1"
                     /db_xref="GeneID:23239"
                     /db_xref="HGNC:HGNC:20610"
                     /db_xref="MIM:609396"
     exon            1..1719
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..5297
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /EC_number="3.1.3.16"
                     /note="pleckstrin homology domain containing, family E
                     (with leucine rich repeats) member 1; suprachiasmatic
                     nucleus circadian oscillatory protein; SCN circadian
                     oscillatory protein; PH domain-containing family E member
                     1; protein phosphatase, Mg2+/Mn2+ dependent 3A"
                     /codon_start=1
                     /product="PH domain leucine-rich repeat-containing protein
                     phosphatase 1"
                     /protein_id="NP_919431.2"
                     /db_xref="CCDS:CCDS45881.2"
                     /db_xref="GeneID:23239"
                     /db_xref="HGNC:HGNC:20610"
                     /db_xref="MIM:609396"
                     /translation="MEPAAAATVQRLPELGREDRASAPAAAAAAAAAAAAAAAALAAA
                     AGGGRSPEPALTPAAPSGGNGSGSGAREEAPGEAPPGPLPGRAGGAGRRRRRGAPQPI
                     AGGAAPVPGAGGGANSLLLRRGRLKRNLSAAAAAASSSSSSSAAAASHSPGAAGLPAS
                     CSASASLCTRSLDRKTLLLKHRQTLQLQPSDRDWVRHQLQRGCVHVFDRHMASTYLRP
                     VLCTLDTTAGEVAARLLQLGHKGGGVVKVLGQGPGAAAAREPAEPPPEAGPRLAPPEP
                     RDSEVPPARSAPGAFGGPPRAPPADLPLPVGGPGGWSRRASPAPSDSSPGEPFVGGPV
                     SSPRAPRPVVSDTESFSLSPSAESVSDRLDPYSSGGGSSSSSEELEADAASAPTGVPG
                     QPRRPGHPAQPLPLPQTASSPQPQQKAPRAIDSPGGAVREGSCEEKAAAAVAPGGLQS
                     TPGRSGVTAEKAPPPPPPPTLYVQLHGETTRRLEAEEKPLQIQNDYLFQLGFGELWRV
                     QEEGMDSEIGCLIRFYAGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCG
                     TCLIVSSVKDSLTGKMHVLPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEY
                     LRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQNPSLPAAR
                     GLNELQRFTKLKSLNLSNNHLGDFPLAVCSIPTLAELNVSCNALRSVPAAVGVMHNLQ
                     TFLLDGNFLQSLPAELENMKQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMSGNCVETL
                     RLQALRKMPHIKHVDLRLNVIRKLIADEVDFLQHVTQLDLRDNKLGDLDAMIFNNIEV
                     LHCERNQLVTLDICGYFLKALYASSNELVQLDVYPVPNYLSYMDVSRNRLENVPEWVC
                     ESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNQLARLPERLERTSVEVLDVQHN
                     QLLELPPNLLMKADSLRFLNASANKLESLPPATLSEETNSILQELYLTNNSLTDKCVP
                     LLTGHPHLKILHMAYNRLQSFPASKMAKLEELEEIDLSGNKLKAIPTTIMNCRRMHTV
                     IAHSNCIEVFPEVMQLPEIKCVDLSCNELSEVTLPENLPPKLQELDLTGNPRLVLDHK
                     TLELLNNIRCFKIDQPSTGDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFCDNREA
                     LYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMVNTFIVMQRKLGTAGQKLG
                     GAAVLCHIKHDPVDPGGSFTLTSANVGKCQTVLCRNGKPLPLSRSYIMSCEEELKRIK
                     QHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSKGLWD
                     SLSVEEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSA
                     GGAVPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTA
                     SDEPPPGALSENSPAYPSEQRCMLHPICLSNSFQRQLSSATFSSAFSDNGLDSDDEEP
                     IEGVFTNGSRVEVEVDIHCSRAKEKEKQQHLLQVPAEASDEGIVISANEDEPGLPRKA
                     DFSAVGTIGRRRANGSVAPQERSHNVIEVATDAPLRKPGGYFAAPAQPDPDDQFIIPP
                     ELEEEVKEIMKHHQEQQQQQQPPPPPQLQPQLPRHYQLDQLPDYYDTPL"
     exon            1720..1916
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2042
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2209
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..2356
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..2587
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2790
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2851
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2947
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3103
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3104..3304
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3305..3467
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3468..3598
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3599..3703
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3704..3898
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            3899..4127
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     exon            4128..6299
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6277..6282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /note="hexamer: AATAAA"
     polyA_site      6299
                     /gene="PHLPP1"
                     /gene_synonym="PHLPP; PLEKHE1; PPM3A; SCOP"
                     /note="major polyA site"
ORIGIN      
        1 cataatcccc tggaacggcc gcgcacaacg ccattggctt ctcccttctc cgcgcgccgc
       61 cgccgtctcc cacctccgcc tcatcgcctc cctctccgcc cgctgcctcc ggagctgggg
      121 gggaaacgcg aagccccact gcaatggagc ccgccgccgc ggccacggta cagcgactcc
      181 ccgagctcgg cagggaggac cgagcttcgg ctccggcggc cgccgctgcg gcagcagcag
      241 cagcagcggc ggccgcggcg gctctggcgg cggcggccgg gggcggccgg agtccggagc
      301 ccgcgctgac cccggcggcc ccgagcggcg ggaacggcag cggcagcggg gcgcgggaag
      361 aggccccagg cgaggcgccg ccggggccgc tgccgggcag agcggggggt gccgggcgca
      421 ggaggcggcg cggggcgccc cagcccattg ccggcggggc tgcccccgta cccggggccg
      481 gcggcggcgc caactccctc ctgctgagga gagggcggct gaagaggaat ctgtccgcgg
      541 ccgccgcggc cgcctcctcg tcgtcgtcgt cctcggccgc tgctgcctcg cactcccccg
      601 gcgctgccgg cctccccgcc tcctgctcgg cctcggcgtc gctgtgcacc cggagcctgg
      661 acaggaagac gctgcttctg aagcaccggc agacgctgca gctgcagccg tcggaccggg
      721 actgggtgag gcaccagctc cagcgcggct gcgtgcacgt cttcgaccgc cacatggcct
      781 cgacctacct gcgcccggtg ctctgcacac tggacaccac ggccggcgag gtggccgccc
      841 gcctgctgca gctgggccac aaaggcggcg gcgtggtgaa ggtgctgggc caggggcccg
      901 gagccgccgc cgcccgggag cccgctgaac cgccccccga ggccggcccc cggctggcgc
      961 ccccggagcc gcgggactcg gaggtaccgc ccgcgaggag cgcgccgggt gccttcgggg
     1021 ggcctccgcg cgcgcccccc gccgacctac ccctgcccgt cggcggcccg ggcgggtggt
     1081 cgcgccgcgc cagcccagcg ccctcggact ccagccccgg cgagccgttc gttgggggcc
     1141 ctgtctcttc gccccgcgcc ccacggcctg tggtctccga caccgagagc ttcagtctga
     1201 gtcccagcgc cgagagcgtg tctgaccggt tggaccccta cagcagcggc ggcggctcct
     1261 cgtcgtcgtc ggaagagctc gaggccgacg cagcctcggc cccgacgggg gtcccgggcc
     1321 agccccgccg tcccggccac cccgcgcagc ccctcccgct tccccagacg gcttcctcgc
     1381 ctcagccgca gcagaaagcc ccgagggcca ttgacagccc gggcggggcc gtccgcgagg
     1441 ggtcgtgcga ggagaaggca gcggcagccg tggccccggg aggcctccag tctacccccg
     1501 ggaggagcgg ggtgaccgcg gagaaggcgc ctccgccgcc cccgccgccc accctgtacg
     1561 tgcagctcca cggagagacc acccggcgct tggaggcgga ggagaagcca ttgcagatcc
     1621 aaaatgacta cctcttccaa ctgggatttg gggagctgtg gagggtgcag gaggaaggca
     1681 tggactcgga gattggctgc ctcatccgct tctatgcagg aaaacctcac agcacgggta
     1741 gctctgaacg gattcagctc tcaggaatgt ataatgtccg taaaggcaag atgcagttgc
     1801 cagtgaaccg atggacaaga cgccaagtca tcctatgtgg gacctgcctg atagtatcat
     1861 ctgtgaaaga cagcttgacc ggaaagatgc atgttctgcc actaattggt ggaaaagtag
     1921 aagaagtgaa aaagcaccaa cactgtttag catttagctc ctctggaccc caaagccaga
     1981 cttactacat ttgctttgat actttcacag aatacttaag gtggctgcga caagtctcca
     2041 aggttgcatc ccagcgcatt agctcagtgg acctctcgtg ttgtagcctg gaacatctgc
     2101 ctgccaacct cttctacagc caagacctca ctcatctcaa tttaaaacaa aacttcctaa
     2161 ggcagaaccc tagccttcca gctgccaggg ggcttaatga actgcaaagg ttcaccaagt
     2221 tgaagagtct taacctttcc aataatcatt taggggactt cccactggca gtctgcagta
     2281 ttccaaccct ggcagagctg aacgtgtcct gcaatgccct gcgatcagtc ccggcagccg
     2341 ttggagtgat gcacaactta cagacatttt tgttggatgg aaactttctc caatcccttc
     2401 ctgctgagtt ggagaacatg aagcagctta gttatctggg tctttctttc aatgaattta
     2461 ctgacattcc cgaagtattg gagaaattga ctgctgtgga taaactttgt atgtctggaa
     2521 actgtgtgga gacccttagg ctacaggctt taagaaaaat gcctcacatt aaacatgtgg
     2581 atctaaggtt gaacgtaatt aggaagctga tagcagatga agtggacttt ctacagcatg
     2641 ttactcagct tgacctacga gacaataagc ttggtgatct agatgctatg attttcaaca
     2701 acattgaagt tttacactgt gaaaggaatc aactggtcac attagacatc tgtggctatt
     2761 tcctaaaagc gctctatgcc tcttctaatg aacttgttca acttgatgtt tacccagttc
     2821 caaattatct gtcctacatg gatgtttcaa ggaaccgctt agaaaatgtg cctgagtggg
     2881 tatgtgaaag ccgaaagcta gaagttttgg atattggcca taatcaaata tgtgaacttc
     2941 ctgcccgctt attttgtaat agcagtctcc ggaaactact ggcaggacac aaccagttgg
     3001 caaggctgcc tgaaaggcta gaaagaacct cggtggaggt cttggatgtg caacacaacc
     3061 agctccttga gctcccacct aaccttctga tgaaggctga cagcctgaga ttcctgaacg
     3121 cctctgcgaa caaactggaa agccttcctc cagccacgct ttccgaagag acaaacagta
     3181 tcttacaaga gttgtatttg acaaataaca gcctcacaga caaatgtgtg cccttgttaa
     3241 cgggacaccc ccatttgaag atccttcaca tggcctataa ccgacttcag agttttccag
     3301 caagtaaaat ggcgaaactg gaggaacttg aagaaattga tctcagtggg aataagctga
     3361 aagccatccc aacaacgatc atgaattgca ggcgcatgca caccgtgatt gctcactcca
     3421 actgcatcga ggtctttccc gaagttatgc agctcccaga gatcaagtgt gtggacctga
     3481 gctgtaatga gctaagtgaa gtcacattac cagaaaacct gcctcccaaa ctgcaggagc
     3541 tagacctgac tggaaacccg cgccttgtcc ttgatcacaa aaccctggaa ctactgaata
     3601 atatccgctg tttcaagatt gatcagcctt ctacaggaga cgcttccgga gccccagctg
     3661 tatggagtca tggttacact gaagcttcgg gggtaaaaaa caagttgtgt gtcgcagccc
     3721 tgtcggtgaa taacttctgt gacaaccgcg aagccctgta tggtgtgttt gacggagacc
     3781 ggaatgtgga ggtgccctac cttctccagt gcactatgag tgacattttg gctgaagagc
     3841 tgcaaaaaac aaaaaacgaa gaagaataca tggtcaatac attcattgtc atgcaaagga
     3901 aacttggaac tgctgggcag aagcttggtg gtgccgctgt cctttgtcat atcaagcatg
     3961 accctgtgga tccaggagga tccttcacct tgacctctgc taatgtgggc aagtgccaaa
     4021 cagttctctg tcgaaatgga aagccgctgc ctctgtccag atcttacatc atgagctgtg
     4081 aagaagagct gaagaggatt aaacagcaca aggccattat cactgaggat ggcaaggtga
     4141 acggagtgac tgagtccacg cgcatcctgg gctacacctt cctccatccc agtgtggtgc
     4201 ctcgccccca cgtgcagtcc gtgctcctga ctccccagga tgagttcttc atcctaggca
     4261 gtaaggggtt gtgggacagc ctgtccgtcg aggaggccgt ggaagccgtg cgcaacgtgc
     4321 ccgatgccct ggctgctgcc aagaagctgt gtaccctggc ccagagctac ggctgccacg
     4381 acagcatcag cgctgtggtg gtgcagctca gtgtcactga ggacagcttc tgctgctgcg
     4441 agctcagcgc cggtggggct gtgccaccac ccagtcctgg catctttcct ccctcagtga
     4501 acatggtgat caaggatcgg ccctcagatg ggctgggcgt gccgtcctcc agcagcggca
     4561 tggcttccga gattagcagt gagctctcca cttctgagat gagcagcgag gtggggtcaa
     4621 cagcctccga tgagcccccg cccggagccc taagcgagaa cagccctgcc taccccagtg
     4681 agcagcgctg catgctccac cccatctgtc tgtccaactc cttccagcgc cagctatcca
     4741 gcgccacgtt ctctagcgcc ttctccgaca acggccttga cagtgacgat gaggagccca
     4801 tcgagggcgt cttcaccaac ggcagccggg tggaggtgga ggtggacatc cactgcagcc
     4861 gggccaagga gaaggagaaa cagcagcacc tgcttcaggt gccagcagag gccagtgatg
     4921 agggcattgt catcagcgcc aacgaggatg agccaggtct gcccaggaag gcagacttct
     4981 ctgccgttgg gaccattggg cgccggaggg ccaatggctc tgttgcgccc caggaaagga
     5041 gccacaatgt gatagaggtg gctacagacg cacctcttcg aaagcctgga ggctattttg
     5101 ctgccccggc tcagccggat cctgatgatc agtttatcat acccccggag ctggaagagg
     5161 aggtcaaaga aatcatgaag catcaccagg agcaacagca gcagcagcag ccgccaccac
     5221 cccctcagct ccagccgcag ctgccgcggc actaccagct ggaccagctg ccagattatt
     5281 acgacacgcc actatgaccc agccgagctg tttaacaaat aaactaacca caaaagactg
     5341 agttgcaaga gtctcccagg ctcacattaa accaggggtt ttactccaca tccttccccc
     5401 agacactgtt cccaacctgt catcgcagct aatctgtagg ttctctttct ttgggttatt
     5461 tttttaagta atcaccactt tcttctagtg atgctttacc aatatgattt acatttgtta
     5521 acttctcccc ctaacatatc agatatgtaa agacaaagaa caaaaggttt aatatattac
     5581 agagaaacag ttaatgataa tgtaatattt tttaaaatgg ctttttgttg tttgtttgga
     5641 aggcagggca ggctgccgtt gctaaatgat ttaataatat tgtaattctg tatttctttg
     5701 gggggaaaag gcttttgttt tgttttgttt tgttttgttt tgtttttgtc ttgaaaataa
     5761 tagacatttg tagaatatgg agactaactc ctaggagttg ctttactctg tcaggtgact
     5821 taagtcactg ggattcacta attttctctg agagaacagc tgattgagaa tttccattgt
     5881 aaatagctca gtgttgtata gtgaggctta cgatgttttg tagtcttggc gtaaggacac
     5941 agcccaagta actgacgttt cccctccccc tcccctctga ggagcctgcc tgcctcacaa
     6001 ctcaccctca cttcactgaa tgaggaggct gagcagctgc agtgtttctg tccggaggaa
     6061 atggatctta ggccactgga caagaacctg cacccaaggg ccctgaaccc attttcctcc
     6121 cctgtcccag ccttcccact ttgacagaca cttttaactg tgttccttac tgctgccaca
     6181 atcagcatgg ttgtatagtg ccccattagg ccatttacat acccagagtt atactcaagc
     6241 agaatgcaca aatggacatg tcataatttt tgttacaata aatatgaaat ttacaagta
//
