LOCUS       NM_001134438            6042 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens pleckstrin homology like domain family B member 2
            (PHLDB2), transcript variant 1, mRNA.
ACCESSION   NM_001134438
VERSION     NM_001134438.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Li,H., Wang,Z., Liang,H., Liu,X., Liu,H., Zhuang,Z. and Hou,J.
  TITLE     Depletion of PHLDB2 Suppresses Epithelial-Mesenchymal Transition
            and Enhances Anti-Tumor Immunity in Head and Neck Squamous Cell
            Carcinoma
  JOURNAL   Biomolecules 14 (2), 232 (2024)
   PUBMED   38397469
  REMARK    GeneRIF: Depletion of PHLDB2 Suppresses Epithelial-Mesenchymal
            Transition and Enhances Anti-Tumor Immunity in Head and Neck
            Squamous Cell Carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Ribolla,L.M., Sala,K., Tonoli,D., Ramella,M., Bracaglia,L.,
            Bonomo,I., Gonnelli,L., Lamarca,A., Brindisi,M., Pierattelli,R.,
            Provenzani,A. and de Curtis,I.
  TITLE     Interfering with the ERC1-LL5beta interaction disrupts plasma
            membrane-Associated platforms and affects tumor cell motility
  JOURNAL   PLoS One 18 (7), e0287670 (2023)
   PUBMED   37437062
  REMARK    GeneRIF: Interfering with the ERC1-LL5beta interaction disrupts
            plasma membrane-Associated platforms and affects tumor cell
            motility.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6042)
  AUTHORS   Luo,M., Huang,Z., Yang,X., Chen,Y., Jiang,J., Zhang,L., Zhou,L.,
            Qin,S., Jin,P., Fu,S., Peng,L., Li,B., Fang,Y., Pu,W., Gong,Y.,
            Liu,Y., Ren,Z., Liu,Q.L., Wang,C., Xiao,F., He,D., Zhang,H., Li,C.,
            Xu,H., Dai,L., Peng,Y., Zhou,Z.G., Huang,C. and Chen,H.N.
  TITLE     PHLDB2 Mediates Cetuximab Resistance via Interacting With EGFR in
            Latent Metastasis of Colorectal Cancer
  JOURNAL   Cell Mol Gastroenterol Hepatol 13 (4), 1223-1242 (2022)
   PUBMED   34952201
  REMARK    GeneRIF: PHLDB2 Mediates Cetuximab Resistance via Interacting With
            EGFR in Latent Metastasis of Colorectal Cancer.
REFERENCE   4  (bases 1 to 6042)
  AUTHORS   Ge,D., Shao,Y., Wang,M., Tao,H., Mu,M. and Tao,X.
  TITLE     RNA-seq-Based Screening in Coal Dust-Treated Cells Identified
            PHLDB2 as a Novel Lung Cancer-Related Molecular Marker
  JOURNAL   Biomed Res Int 2021, 1978434 (2021)
   PUBMED   34337001
  REMARK    GeneRIF: RNA-seq-Based Screening in Coal Dust-Treated Cells
            Identified PHLDB2 as a Novel Lung Cancer-Related Molecular Marker.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6042)
  AUTHORS   Kang,W., Zhang,J., Huang,T., Zhou,Y., Wong,C.C., Chan,R.C.K.,
            Dong,Y., Wu,F., Zhang,B., Wu,W.K.K., Chan,M.W.Y., Cheng,A.S.L.,
            Yu,J., Wong,N., Lo,K.W. and To,K.F.
  TITLE     NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric
            carcinogenesis by upregulating PHLDB2 expression and activating Akt
            pathway
  JOURNAL   Oncogene 40 (9), 1578-1594 (2021)
   PUBMED   33452458
  REMARK    GeneRIF: NOTCH3, a crucial target of miR-491-5p/miR-875-5p,
            promotes gastric carcinogenesis by upregulating PHLDB2 expression
            and activating Akt pathway.
REFERENCE   6  (bases 1 to 6042)
  AUTHORS   Lansbergen,G., Grigoriev,I., Mimori-Kiyosue,Y., Ohtsuka,T.,
            Higa,S., Kitajima,I., Demmers,J., Galjart,N., Houtsmuller,A.B.,
            Grosveld,F. and Akhmanova,A.
  TITLE     CLASPs attach microtubule plus ends to the cell cortex through a
            complex with LL5beta
  JOURNAL   Dev Cell 11 (1), 21-32 (2006)
   PUBMED   16824950
  REMARK    GeneRIF: LL5beta and ELKS can form a PIP3-regulated cortical
            platform to which CLASPs attach distal microtubule ends.
REFERENCE   7  (bases 1 to 6042)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   8  (bases 1 to 6042)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   9  (bases 1 to 6042)
  AUTHORS   Paranavitane,V., Coadwell,W.J., Eguinoa,A., Hawkins,P.T. and
            Stephens,L.
  TITLE     LL5beta is a phosphatidylinositol (3,4,5)-trisphosphate sensor that
            can bind the cytoskeletal adaptor, gamma-filamin
  JOURNAL   J Biol Chem 278 (2), 1328-1335 (2003)
   PUBMED   12376540
REFERENCE   10 (bases 1 to 6042)
  AUTHORS   Dowler,S., Currie,R.A., Campbell,D.G., Deak,M., Kular,G.,
            Downes,C.P. and Alessi,D.R.
  TITLE     Identification of pleckstrin-homology-domain-containing proteins
            with novel phosphoinositide-binding specificities
  JOURNAL   Biochem J 351 (Pt 1), 19-31 (2000)
   PUBMED   11001876
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA438817.1, AK315050.1, AC060225.12 and AW264132.1.
            
            On May 17, 2019 this sequence version replaced NM_001134438.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a). Variants 1 and 2
            both encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK315050.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000431670.7/ ENSP00000405405.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                DA438817.1         86-98
            14-911              AK315050.1         11-908
            912-912             AC060225.12        37453-37453
            913-2147            AK315050.1         910-2144
            2148-2148           AC060225.12        71964-71964
            2149-4088           AK315050.1         2145-4084
            4089-6014           AC060225.12        127354-129279
            6015-6042           AW264132.1         18-45               c
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.2"
     gene            1..6042
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /note="pleckstrin homology like domain family B member 2"
                     /db_xref="GeneID:90102"
                     /db_xref="HGNC:HGNC:29573"
                     /db_xref="MIM:610298"
     exon            1..312
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            313..1661
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     CDS             327..4088
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /note="isoform a is encoded by transcript variant 1; LL5
                     beta; protein LL5-beta"
                     /codon_start=1
                     /product="pleckstrin homology-like domain family B member
                     2 isoform a"
                     /protein_id="NP_001127910.1"
                     /db_xref="CCDS:CCDS46886.1"
                     /db_xref="GeneID:90102"
                     /db_xref="HGNC:HGNC:29573"
                     /db_xref="MIM:610298"
                     /translation="MEEHSYIQKELDLQNGSLEEDSVVHSVENDSQNMMESLSPKKYS
                     SSLRFKANGDYSGSYLTLSQPVPAKRSPSPLGTSVRSSPSLAKIQGSKQFSYDGTDKN
                     IPMKPPTPLLNTTSSLSGYPLGRADFDHYTGRDSERALRLSEKPPYSKYSSRHKSHDN
                     VYSLGGLEGRKASGSLLAMWNGSSLSDAGPPPISRSGAASMPSSPKQARKMSIQDSLA
                     LQPKLTRHKELASENINLRTRKYSSSSLSHMGAYSRSLPRLYRATENQLTPLSLPPRN
                     SLGNSKRTKLGEKDLPHSVIDNDNYLNFSSLSSGALPYKTSASEGNPYVSSTLSVPAS
                     PRVARKMLLASTSSCASDDFDQASYVGTNPSHSLLAGESDRVFATRRNFSCGSVEFDE
                     ADLESLRQASGTPQPALRERKSSISSISGRDDLMDYHRRQREERLREQEMERLERQRL
                     ETILSLCAEYTKPDSRLSTGTTVEDVQKINKELEKLQLSDEESVFEEALMSPDTRYRC
                     HRKDSLPDADLASCGSLSQSSASFFTPRSTRNDELLSDLTRTPPPPSSTFPKASSESS
                     YLSILPKTPEGISEEQRSQELAAMEETRIVILNNLEELKQKIKDINDQMDESFRELDM
                     ECALLDGEQKSETTELMKEKEILDHLNRKIAELEKNIVGEKTKEKVKLDAEREKLERL
                     QELYSEQKTQLDNCPESMREQLQQQLKRDADLLDVESKHFEDLEFQQLEHESRLDEEK
                     ENLTQQLLREVAEYQRNIVSRKEKISALKKQANHIVQQAQREQDHFVKEKNNLIMMLQ
                     REKENLCNLEKKYSSLSGGKGFPVNPNTLKEGYISVNEINEPCGNSTNLSPSTQFPAD
                     ADAVATEPATAVLASQPQSKEHFRSLEERKKQHKEGLYLSDTLPRKKTTSSISPHFSS
                     ATMGRSITPKAHLPLGQSNSCGSVLPPSLAAMAKDSESRRMLRGYNHQQMSEGHRQKS
                     EFYNRTASESNVYLNSFHYPDHSYKDQAFDTLSLDSSDSMETSISACSPDNISSASTS
                     NIARIEEMERLLKQAHAEKTRLLESREREMEAKKRALEEEKRRREILEKRLQEETSQR
                     QKLIEKEVKIRERQRAQARPLTRYLPVRKEDFDLRSHVETAGHNIDTCYHVSITEKTC
                     RGFLIKMGGKIKTWKKRWFVFDRNKRTFSYYADKHETKLKGVIYFQAIEEVYYDHLKN
                     ANKSPNPLLTFSVKTHDRIYYMVAPSPEAMRIWMDVIVTGAEGYTHFLL"
     exon            1662..2045
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2189
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2190..2327
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2328..2456
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2457..2612
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2613..2723
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2724..2813
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2814..2957
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            2958..3098
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            3099..3198
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3403
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            3404..3494
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..3641
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            3642..3861
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..3947
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     exon            3948..6042
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5756..5761
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /note="hexamer: ATTAAA"
     regulatory      5787..5792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /note="hexamer: AATAAA"
     polyA_site      5788
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /note="major polyA site"
     polyA_site      5814
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /note="major polyA site"
     regulatory      5970..5975
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
                     /note="hexamer: AATGAA"
     polyA_site      5985
                     /gene="PHLDB2"
                     /gene_synonym="LL5b; LL5beta"
ORIGIN      
        1 actgcgttgc tgcgaacgta gctttgagaa gctggcgccc agtgatgggg caaacagcca
       61 tgcccttcca gcagccgtga aagccccaac agcaaactgc ctgggagcgg ggcaggtcac
      121 caacttcgtt gctcgaactc cctgggtgcc cgccgcggtg gttacaaagg gggtcaagag
      181 tgccggaccc agccgcgcgg agcccaccat tgcggcccga gggggacccg acgggggccc
      241 gacggtgtgg cgtggcgcaa ggagcgcgcc tgccttctct cgccgccggg aacgggctgc
      301 accaatggcc agattccagc aagattatgg aagagcatag ctacatacaa aaggagctag
      361 atttacaaaa tggtagctta gaggaagact ctgtggtgca ttctgttgag aacgattccc
      421 aaaacatgat ggagagcctc agcccaaaga aatactcttc cagtctgaga tttaaagcca
      481 atggagacta ttctggctcc tatttaaccc tctcacaacc tgtgcctgca aagagaagcc
      541 cttctccttt gggaaccagt gtcagaagca gcccctcctt agccaaaatc cagggaagca
      601 agcagttctc ttatgatgga actgacaaaa atattcctat gaaacctcca actcctttac
      661 tcaacactac atcctccctc agtggatatc cacttggaag agcagacttt gatcattata
      721 ctggccggga cagtgaaagg gccttgaggc tctcagagaa gcctccctat tccaaatata
      781 gctcaaggca taaatcgcat gacaatgtct actctcttgg agggctggaa ggtcggaagg
      841 catctggctc gctcctggcc atgtggaatg gaagttccct gagtgatgct ggcccgcctc
      901 ctatcagcag atcgggagcc gcaagcatgc cttcaagccc aaagcaagcc aggaaaatga
      961 gcattcagga cagcctggcg cttcaaccca agttaactag acacaaggag cttgcatctg
     1021 aaaacatcaa tttgagaact aggaagtact ccagcagcag cctgagtcac atgggagcct
     1081 acagccgatc acttcccagg ttgtacagag ccacagagaa ccagctgaca cctctcagct
     1141 tgcctccaag aaactctctg ggcaattcca aacgaacaaa acttggggaa aaggatctac
     1201 ctcatagcgt aatagacaat gacaattacc ttaatttttc ttctttgagc tcaggggctt
     1261 taccctataa aacctctgct tctgaaggca atccttatgt aagttctacc ctcagtgtcc
     1321 ctgccagtcc acgagtggct cggaagatgc ttctggcctc cacctcctcc tgtgcctctg
     1381 atgactttga tcaggcttca tatgtgggga caaacccgag tcattcactt cttgctggag
     1441 agtcagacag agtttttgcg accaggagga acttctcttg tggatctgtg gaatttgatg
     1501 aggcagattt ggaaagcctc agacaggcct caggaacccc ccagcctgcc cttcgggaac
     1561 ggaaaagcag tattagctcc atttcaggac gtgatgacct gatggattat caccggcggc
     1621 agagggagga aagactcagg gagcaggaaa tggagcgatt ggagagacag cgtctggaga
     1681 ccatcctcag tctctgtgct gaatacacaa agcctgacag tcgcttatct actgggacca
     1741 ccgtggaaga tgtgcagaaa atcaacaagg agcttgagaa gctgcagctc tctgatgagg
     1801 agtctgtgtt tgaggaagcc ctcatgagcc ctgacacaag atacaggtgc caccggaaag
     1861 actccctccc tgatgcagac ttggcaagct gtgggagtct cagtcagagc agtgccagct
     1921 tctttacccc caggagcacc aggaatgatg aactactcag tgacctcacc cggactcctc
     1981 caccaccatc ctccaccttt ccgaaagctt ccagcgagtc ctcttatcta agtatcctac
     2041 caaagacccc agagggtata agtgaagaac agagatctca ggagttggct gcaatggaag
     2101 aaacccggat agtcattctg aacaacctcg aggaacttaa gcaaaaaatc aaagacataa
     2161 atgatcagat ggatgagtct ttcagagagt tggatatgga atgtgctctt ttggatggag
     2221 aacagaaatc tgaaacaact gaacttatga aggagaagga gattttggat catctaaacc
     2281 ggaaaatagc tgaactggaa aagaacattg ttggtgaaaa gaccaaggag aaggtaaagc
     2341 ttgatgctga aagggaaaaa ctagagaggc ttcaggagct ttactccgag cagaagaccc
     2401 agctggacaa ttgtcctgag tccatgaggg aacagttaca acaacaactg aagagggatg
     2461 ctgacctgtt ggatgttgaa agcaaacact ttgaagacct ggagttccag cagcttgaac
     2521 atgagagccg tctagatgaa gaaaaggaga acttgactca acagctcctg cgtgaagttg
     2581 ctgaatatca acggaacatc gtttctagaa aggaaaaaat ttctgcattg aaaaagcaag
     2641 ccaatcacat tgttcagcag gctcagagag agcaagatca ttttgtgaaa gaaaagaata
     2701 atttaataat gatgttgcaa agagaaaagg agaatctttg taatttggaa aagaaatact
     2761 ccagcctctc tggggggaaa gggtttcccg ttaaccccaa tactttaaaa gagggctata
     2821 tcagtgtaaa tgagattaat gagccgtgtg gcaattccac gaatctatcc ccttccactc
     2881 agtttcctgc tgatgctgat gctgttgcca ctgagcctgc cacagctgtg ctggcgagcc
     2941 agccacagag taaagagcac tttagaagtc tggaagaaag gaaaaaacag cataaagaag
     3001 gcctctatct gagtgatact ttgcctcgaa agaaaaccac atcttccatc tccccacatt
     3061 tcagcagtgc tactatgggg agaagcatca ccccaaaggc ccatctgccc ctaggacaga
     3121 gtaacagctg tggaagtgtg ctccctccct cactggcagc catggccaaa gactcagaat
     3181 ctcggaggat gctcagaggt tataatcacc aacagatgag tgaaggacac aggcagaaat
     3241 ctgaatttta taaccgcaca gcatctgaat caaatgtcta cttgaatagt ttccattatc
     3301 cagatcacag ctacaaggac caggcctttg atactctgag cctcgatagc tctgatagca
     3361 tggagaccag catctctgct tgctcaccag acaacatctc tagtgccagc acttcaaata
     3421 ttgctagaat agaagaaatg gagagacttt tgaagcaggc tcatgcagaa aagacgcggc
     3481 tgctcgaatc cagggaacgg gaaatggaag ccaaaaaacg agccctggaa gaagaaaaac
     3541 gacgccggga aatcctggaa aaacgattac aggaagaaac tagccagagg cagaagttaa
     3601 tagaaaagga agtaaaaata agggagagac aaagggcaca ggctcgtcct ttgacacgct
     3661 acctgcctgt ccggaaggaa gactttgatt tgcggagcca tgtagagact gctggccaca
     3721 atattgacac ctgttaccat gtatcaatca cagagaagac ctgccgagga ttcctcatca
     3781 aaatgggtgg gaaaattaaa acgtggaaaa aacgttggtt tgtttttgat cggaacaagc
     3841 gaacattctc ttattatgca gacaagcatg aaactaaatt gaaaggagta atatactttc
     3901 aagccattga agaagtctat tatgatcacc tcaagaatgc taataagagt cctaatccgt
     3961 tactcacctt tagcgtcaag actcatgaca gaatctatta tatggtagcc ccatcgccag
     4021 aagccatgcg gatctggatg gatgttatag ttacgggggc agaaggttac actcacttct
     4081 tgttgtagtg aactgaggca acagtccact tcagggcaga cggcaataat ctcttacaag
     4141 aatgaagcca tattcaaccc cagatgagaa aacccaacag atccatccct tgagctgtaa
     4201 acactcagaa ctcctttcat atcaagacaa gttatttgta aaaaataaag aaggggtttt
     4261 aatacaaacc ttcataataa atagcaaaat aattgaagct tccatgagaa agaaaacact
     4321 attttgataa attggatcac ttataggaac atttctaata aactgttttt aatcagttgt
     4381 cggatttggt gaaataaact aaacaggtta cagaatatct gtatgtactt ggaaatacag
     4441 aataacttta tcacccaaat cactggcatt gacattattg gtaatcaact ggcttttttt
     4501 aaaaaaggta gcattttgtt gacagttatt ttgtaaacat aagcaaataa gggcttggag
     4561 ggaaatacat tttaggagga gttttgcctt aattttttaa gtactgcacc aaaaccaaag
     4621 agctgacctg acttctgtgg aacagtagta actgcaagtg atgaactgca tttcgtattg
     4681 ttctgtatat ttcaaaatgg tattttgatg ccatcaaatg cccaggaaat tgactttgca
     4741 gtgtcaccac tggtgtaagc tactatatat gtatatatat atgtagtaaa ccactttttg
     4801 taaaagaaga aagagcaaaa agctgtgtgt tttagaaaaa aaagccaggt tacgcaacag
     4861 gcattctgtc atgttgaaca attttaaata aagagaatat ctggtgttag gagcttgttt
     4921 tgctgaagat ttctccattc ctggtgctga gaataaaggc aaccagtagc caatgtcctt
     4981 tagattgtct gatttctttt tgttgtggag cacacctgct aactgctccc tcaacataac
     5041 tatgaaatca tagctctgtt ttcaccaaag aacagaccaa ttaacatact tatttgcaga
     5101 agtggtgtag ttctataaaa cggcaaatga agttcaactt aatattctct ataatgtatt
     5161 attttatttt attttttaca attagccttt tttttagtta atttttgtca aatgaaacga
     5221 cttcaggcaa gtctctttta taatggtttt tcaagtgcca tttattctag tttatcatgt
     5281 tttgcatgtt tgaaagtatg aatgtgctct ttcctaaaac atggcaaatg aatagatgta
     5341 gagaataaca atattactta caagatgaaa tgattagatt agaagtgtcc ctttattaaa
     5401 ctttgtcagc ctgactgggt acaattcttt tgttaatttg cagtgtggtt tgtatacacg
     5461 tatacgtgtt atcaataata agattttgca actggatgac acaagatttt acttgaacag
     5521 tgaaggacaa aaatcatgat tgtggaagat atttttaaaa tctgattttg cagcgatcac
     5581 ttttaaaccc tgtagtgatg taagactaaa atataattgc taagattttg ttggttaatg
     5641 taaagatatg acttttctgc actgtactct cttcatagga ttgtaaaggt gttctaatcc
     5701 aattgcatga tgtagtaagc ctcttaaata tgtgtgttaa atatattgag tttggattaa
     5761 aatgttgaca tgatttcaca tttgaaaata aactcatctc ttattttgaa gttacctatc
     5821 tgtagtatga tggaggatga attaatcgca aatgacagtt gtagaaacta tgtaaagttt
     5881 gttgtgtgct aacattatga tttgtagtgt ataaactgaa gtattccaat agaagtatct
     5941 ctggttacat cctattgctt acaaaatgaa atgaaccctg aaaaactctg acatcatatt
     6001 gttgtctgtt tacacagata catttattgc tggggattgc aa
//
