LOCUS       NM_012396               2749 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens pleckstrin homology like domain family A member 3
            (PHLDA3), transcript variant 1, mRNA.
ACCESSION   NM_012396
VERSION     NM_012396.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Saito,M., Sada,A., Fukuyo,M., Aoki,K., Okumura,K., Tabata,Y.,
            Chen,Y., Kaneda,A., Wakabayashi,Y. and Ohki,R.
  TITLE     PHLDA3 Is an Important Downstream Mediator of p53 in Squamous Cell
            Carcinogenesis
  JOURNAL   J Invest Dermatol 142 (4), 1040-1049 (2022)
   PUBMED   34592332
  REMARK    GeneRIF: PHLDA3 Is an Important Downstream Mediator of p53 in
            Squamous Cell Carcinogenesis.
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Ma,S., Quan,P., Yu,C., Fan,X., Yang,S., Jia,W., Zhang,L., Wang,F.,
            Liu,F., Yang,L., Qin,W. and Yang,X.
  TITLE     PHLDA3 exerts an antitumor function in prostate cancer by
            down-regulating Wnt/beta-catenin pathway via inhibition of Akt
  JOURNAL   Biochem Biophys Res Commun 571, 66-73 (2021)
   PUBMED   34303965
  REMARK    GeneRIF: PHLDA3 exerts an antitumor function in prostate cancer by
            down-regulating Wnt/beta-catenin pathway via inhibition of Akt.
REFERENCE   3  (bases 1 to 2749)
  AUTHORS   R Mangone,F., Av Valoyes,M., G do Nascimento,R., Pf Conceicao,M., R
            Bastos,D., C Pavanelli,A., C Soares,I., S de Mello,E., Nonogaki,S.,
            Ab de T Osorio,C. and A Nagai,M.
  TITLE     Prognostic and predictive value of Pleckstrin homology-like domain,
            family A family members in breast cancer
  JOURNAL   Biomark Med 14 (16), 1537-1552 (2020)
   PUBMED   33179538
  REMARK    GeneRIF: Prognostic and predictive value of Pleckstrin
            homology-like domain, family A family members in breast cancer.
REFERENCE   4  (bases 1 to 2749)
  AUTHORS   Bensellam,M., Chan,J.Y., Lee,K., Joglekar,M.V., Hardikar,A.A.,
            Loudovaris,T., Thomas,H.E., Jonas,J.C. and Laybutt,D.R.
  TITLE     Phlda3 regulates beta cell survival during stress
  JOURNAL   Sci Rep 9 (1), 12827 (2019)
   PUBMED   31492921
  REMARK    GeneRIF: Phlda3 regulates beta cell survival during stress.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2749)
  AUTHORS   Liu,J., Liu,X., Hui,X., Cai,L., Li,X., Yang,Y., Shu,S., Wang,F.,
            Xia,H. and Li,S.
  TITLE     Novel Role for Pleckstrin Homology-Like Domain Family A, Member 3
            in the Regulation of Pathological Cardiac Hypertrophy
  JOURNAL   J Am Heart Assoc 8 (16), e011830 (2019)
   PUBMED   31426686
  REMARK    GeneRIF: Novel Role for Pleckstrin Homology-Like Domain Family A,
            Member 3 in the Regulation of Pathological Cardiac Hypertrophy.
REFERENCE   6  (bases 1 to 2749)
  AUTHORS   Muroi,H., Nakajima,M., Satomura,H., Takahashi,M., Yamaguchi,S.,
            Sasaki,K., Yokobori,T., Miyazaki,T., Kuwano,H. and Kato,H.
  TITLE     Low PHLDA3 expression in oesophageal squamous cell carcinomas is
            associated with poor prognosis
  JOURNAL   Anticancer Res 35 (2), 949-954 (2015)
   PUBMED   25667479
  REMARK    GeneRIF: Low PHLDA3 expression in ESCC may be predictive of tumor
            recurrence suggesting that Akt activation may be a therapeutic
            target in ESCCs
REFERENCE   7  (bases 1 to 2749)
  AUTHORS   Ohki,R., Saito,K., Chen,Y., Kawase,T., Hiraoka,N., Saigawa,R.,
            Minegishi,M., Aita,Y., Yanai,G., Shimizu,H., Yachida,S., Sakata,N.,
            Doi,R., Kosuge,T., Shimada,K., Tycko,B., Tsukada,T., Kanai,Y.,
            Sumi,S., Namiki,H., Taya,Y., Shibata,T. and Nakagama,H.
  TITLE     PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine
            tumors
  JOURNAL   Proc Natl Acad Sci U S A 111 (23), E2404-E2413 (2014)
   PUBMED   24912192
  REMARK    GeneRIF: Results indicate that tumor suppressor PHLDA3-mediated
            pathway is important in the development of pancreatic
            neuroendocrine tumors (PanNETs).
REFERENCE   8  (bases 1 to 2749)
  AUTHORS   Yoo,N.J., Kim,Y.R. and Lee,S.H.
  TITLE     Expressional and mutational analysis of PHLDA3 gene in common human
            cancers
  JOURNAL   Pathology 43 (5), 510-511 (2011)
   PUBMED   21753719
  REMARK    GeneRIF: findings indicate somatic mutation of PHLDA3 is rare in
            common cancer types; PHLDA3 expression was lost in 22% of prostate
            cancers; these results indicate that PHLDA3 are altered in prostate
            cancers by loss of expression
REFERENCE   9  (bases 1 to 2749)
  AUTHORS   Kawase,T., Ohki,R., Shibata,T., Tsutsumi,S., Kamimura,N.,
            Inazawa,J., Ohta,T., Ichikawa,H., Aburatani,H., Tashiro,F. and
            Taya,Y.
  TITLE     PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt
  JOURNAL   Cell 136 (3), 535-550 (2009)
   PUBMED   19203586
  REMARK    GeneRIF: Study identifies PHLDA3 as a p53 target gene that encodes
            a PH domain-only protein and finds that PHLDA3 competes with the PH
            domain of Akt for binding of membrane lipids, inhibiting Akt
            translocation to the cellular membrane and activation.
REFERENCE   10 (bases 1 to 2749)
  AUTHORS   Frank,D., Mendelsohn,C.L., Ciccone,E., Svensson,K., Ohlsson,R. and
            Tycko,B.
  TITLE     A novel pleckstrin homology-related gene family defined by
            Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression,
            chromosomal location, and parental imprinting
  JOURNAL   Mamm Genome 10 (12), 1150-1159 (1999)
   PUBMED   10594239
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC096677.2, BC068273.1, BC014390.1, AF151100.1, BE778799.1,
            BX090314.1, BE892338.1, BQ000725.1, AI624156.1 and BC033874.1.
            
            On May 17, 2019 this sequence version replaced NM_012396.4.
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            CCDS Note: This CCDS ID represents the protein described in PMID:
            19203586 and is supported by BC014390.1. It should be noted this
            transcript is predicted to undergo nonsense-mediated mRNA decay
            (NMD). However, the protein is represented because it was detected
            endogenously in PMID: 19203586. It is likely that the majority of
            transcripts representing this variant will undergo NMD, while some
            low level of NMD escape may allow for the expression of this
            protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.126849.1,
                                           SRR1803616.230560.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367311.5/ ENSP00000356280.3
            NMD candidate          :: PMID: 19203586
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AC096677.2         67170-67186         c
            18-317              BC068273.1         1-300
            318-839             BC014390.1         153-674
            840-1173            AF151100.1         757-1090
            1174-1531           BC014390.1         1009-1366
            1532-1533           BC068273.1         1516-1517
            1534-1758           AC096677.2         63267-63491         c
            1759-1770           BE778799.1         1-12
            1771-1774           AC096677.2         63251-63254         c
            1775-1864           BE778799.1         17-106
            1865-1955           BX090314.1         1-91
            1956-1956           BE892338.1         80-80
            1957-1994           BX090314.1         93-130
            1995-1995           BE892338.1         121-121
            1996-2206           BX090314.1         132-342
            2207-2522           BQ000725.1         19-334              c
            2523-2624           AI624156.1         128-229             c
            2625-2625           BC033874.1         142-142
            2626-2743           AI624156.1         9-126               c
            2744-2749           AC096677.2         62276-62281         c
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..2749
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="pleckstrin homology like domain family A member 3"
                     /db_xref="GeneID:23612"
                     /db_xref="HGNC:HGNC:8934"
                     /db_xref="MIM:607054"
     exon            1..848
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             403..786
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="pleckstrin homology-like domain, family A, member
                     2; TDAG51/Ipl homolog 1"
                     /codon_start=1
                     /product="pleckstrin homology-like domain family A member
                     3"
                     /protein_id="NP_036528.1"
                     /db_xref="CCDS:CCDS1412.1"
                     /db_xref="GeneID:23612"
                     /db_xref="HGNC:HGNC:8934"
                     /db_xref="MIM:607054"
                     /translation="MTAAATATVLKEGVLEKRSGGLLQLWKRKRCVLTERGLQLFEAK
                     GTGGRPKELSFARIKAVECVESTGRHIYFTLVTEGGGEIDFRCPLEDPGWNAQITLGL
                     VKFKNQQAIQTVRARQSLGTGTLVS"
     exon            849..2749
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1501..1506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="hexamer: AATGAA"
     polyA_site      1535
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
     regulatory      2500..2505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="hexamer: AATAAA"
     polyA_site      2522
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="major polyA site"
     regulatory      2607..2612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="hexamer: ATTACA"
     regulatory      2623..2628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="hexamer: TATAAA"
     polyA_site      2630
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
     polyA_site      2644
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
     regulatory      2690..2695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
                     /note="hexamer: AATAAG"
     polyA_site      2737
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
     polyA_site      2749
                     /gene="PHLDA3"
                     /gene_synonym="TIH1"
ORIGIN      
        1 aggcgagcgg cggaacatgt aagggcacat cccgcgagct gccgcccagc gcgcagacag
       61 agcccagggg agcaagagaa cgggcgggcg gtggggctca cggcctaggg aggcgcggag
      121 gcatctggca gaggcgggtc gggctgggcc agctggggta gagcggagga gcgggtgccg
      181 gctgaagcgg ggcggtgggc gcggagcgcg ctgggggcac cgacaccacc tcaccggcag
      241 ccgggtgctg agggccgcgg tgtgggtgcg cggagcagtc agggcgcagg tgggcagcgc
      301 gcacggcctg ccagcccggg gcgccagaat cctgcgctgc ggggccgaga ggggcgccgc
      361 gcccgccgca gcctggagct ttccgcgaac ctcggggcgc ccatgacggc ggcggcgacg
      421 gctaccgtgc tcaaggaggg cgtgctggag aagcgcagcg gcgggctgct gcagctgtgg
      481 aagcggaagc gctgcgtcct caccgaacgc gggctgcagc tcttcgaggc caagggcacg
      541 ggcggccggc ccaaggagct cagcttcgcc cgcatcaagg ccgtggagtg cgtggagagc
      601 accgggcgcc acatctactt cacgctggtg accgaagggg gcggcgagat cgacttccgc
      661 tgccccctgg aagatcccgg ctggaacgcc cagatcaccc taggcctggt caagttcaag
      721 aaccagcagg ccatccagac agtgcgggcc cggcagagcc tcgggaccgg gaccctcgtg
      781 tcctaaacca ccgggcgcac catctttcct tcatgctacc caccacctca gtgctgaggt
      841 caaggcagct tcgttgttcc ctctggcttg tgggggcacg gctgtgctcc atgtggcaag
      901 gtggaaggca tggacgtgtg gaggaggcgc tggagctgaa ggaatggacg agccctggga
      961 ggagggcaga aggctacgca gggctgagga tgaagatgca gcccctggat ggtcccagac
     1021 tctcaggaca tgcccagctc aggggcttcg agccacaggc ctggcctcat atggcatgag
     1081 ggggagctgg cataggagcc ccctccctgc tgtggtcctg ccctctgtcc tgcagactgc
     1141 tcttagcccc ctggctttgt gccaggcctg gaggagggca gtcccccatg gggtgccgag
     1201 ccaacgcctc aggaatcagg aggccagcct ggtaccaaaa ggagtaccca gggcctggta
     1261 cccaggccca ctccagaatg gcctctggac tcaccttgag aagggggagc tgctgggcct
     1321 aaagcccact cctgggggtc tcctgctgct taggtccttt tgggaccccc acccatccag
     1381 gccctttctt tgcacacttc ttcccccacc tctacgcatc ttccccccac tgcggtgttc
     1441 ggcctgaagg tggtgggggt gagggggggt ttggccatta gcatttcatg tctttcccca
     1501 aatgaagatg ccctgcaaag ggcagtaacc acacagtttg ctgagtgaaa ttctttgccc
     1561 acaaagaccc agggcttgga gcatctctct ctttatcctt aatctcccaa tttgcaaccc
     1621 attctgaagt ctccagcctg gataggcaga gacactaaaa ccccagtaga cagggccttg
     1681 gtggtcctag gaaggagggc ccatagattc tggaccgagg gatgtcaagc tggtcagttc
     1741 agtccctatg aatgtctctt ctacaggagg ctaccctgcc cctgctaccc tgggagaagc
     1801 ctcagctttc tgggcagagt ttgtctccct gtcatttata ctctcaggct ttatacattt
     1861 acacagtaag ttctccctcc tggagggtta aaaggaataa tttcaacagg gtgaaggcct
     1921 ggcacggtgg ctcacaactg taatccaagg actttgggag gctgaggtgg gtggatcacc
     1981 tgaggtcagg aatttgagac cagcctggcc aacttggtga aaccctgtct ctactaaaaa
     2041 caaaaattag ccaggtgagg tggcacacac ctatagcccc agctactggg ggaggctgag
     2101 gcaggagaat tgcttgaacc tgggaggcag aggttacagt gagctgagat ggcaccactg
     2161 cactccagcc taggtgacaa agcagcaaga cgcagtctca aaacaacaac aacaaaaaaa
     2221 acgggaaaac aggtacagac aatagctgcc tggagttgta cagaaacttg attgggtaac
     2281 cctggacctt tccaggctgt ggccagcagt tgaccctgcc tgcctttcct ccattgtttt
     2341 cccatgtctg accttccctg ttgcaaagca gtggcctact acagggtctc tctggaggga
     2401 gcaaggaggc tcagtggccc cattcagcaa ttcgaagtcc ctttaattgt tttgtgcttc
     2461 caacctgttt tgttcccgtt cagatttctc tcgccttcca ataaaaactt ccctgtcctg
     2521 tgccccagtc cctcagagtc cacaagaggt tcaacttgct catctgatgt gtgaattaca
     2581 tcacacagtt gaatgagaat ttgttaatta caacagaact tttataaata tgtcacttac
     2641 tgtatccaat tgtacaattg tccatgggtg gcttaatccc accatggcaa ataagcgtct
     2701 gtttatgcaa ccctaatcag tgctgagaaa gatcagagct caactagaa
//
