LOCUS       NM_000293               5459 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens phosphorylase kinase regulatory subunit beta (PHKB),
            transcript variant 1, mRNA.
ACCESSION   NM_000293
VERSION     NM_000293.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Yang,W., Zhang,C., Li,Y., Jin,A., Sun,Y., Yang,X., Wang,B. and
            Guo,W.
  TITLE     Phosphorylase Kinase beta Represents a Novel Prognostic Biomarker
            and Inhibits Malignant Phenotypes of Liver Cancer Cell
  JOURNAL   Int J Biol Sci 15 (12), 2596-2606 (2019)
   PUBMED   31754332
  REMARK    GeneRIF: PHKB acts as a novel prognostic indicator for HCC, which
            exerts its suppression function via inactivating AKT and STAT3.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5459)
  AUTHORS   Wang,G., Shen,W., Liu,C.Y., Liu,Y., Wu,T., Cui,X., Yu,T., Zhu,Y.,
            Song,J., Du,P. and Cui,L.
  TITLE     Phosphorylase kinase beta affects colorectal cancer cell growth and
            represents a novel prognostic biomarker
  JOURNAL   J Cancer Res Clin Oncol 143 (6), 971-980 (2017)
   PUBMED   28275865
  REMARK    GeneRIF: PHKb affects colorectal cancer cell growth and represents
            a novel prognostic biomarker.
REFERENCE   4  (bases 1 to 5459)
  AUTHORS   Terashima,M., Fujita,Y., Togashi,Y., Sakai,K., De Velasco,M.A.,
            Tomida,S. and Nishio,K.
  TITLE     KIAA1199 interacts with glycogen phosphorylase kinase beta-subunit
            (PHKB) to promote glycogen breakdown and cancer cell survival
  JOURNAL   Oncotarget 5 (16), 7040-7050 (2014)
   PUBMED   25051373
  REMARK    GeneRIF: Analysis showed that the glycogen phosphorylase kinase
            beta-subunit (PHKB) interacted with the C-terminal region of
            KIAA1199 protein.
REFERENCE   5  (bases 1 to 5459)
  AUTHORS   Schlafli,P., Troger,J., Eckhardt,K., Borter,E., Spielmann,P. and
            Wenger,R.H.
  TITLE     Substrate preference and phosphatidylinositol monophosphate
            inhibition of the catalytic domain of the Per-Arnt-Sim domain
            kinase PASKIN
  JOURNAL   FEBS J 278 (10), 1757-1768 (2011)
   PUBMED   21418524
REFERENCE   6  (bases 1 to 5459)
  AUTHORS   van den Berg,I.E., van Beurden,E.A., de Klerk,J.B., van
            Diggelen,O.P., Malingre,H.E., Boer,M.M. and Berger,R.
  TITLE     Autosomal recessive phosphorylase kinase deficiency in liver,
            caused by mutations in the gene encoding the beta subunit (PHKB)
  JOURNAL   Am J Hum Genet 61 (3), 539-546 (1997)
   PUBMED   9326319
REFERENCE   7  (bases 1 to 5459)
  AUTHORS   Burwinkel,B., Maichele,A.J., Aagenaes,O., Bakker,H.D., Lerner,A.,
            Shin,Y.S., Strachan,J.A. and Kilimann,M.W.
  TITLE     Autosomal glycogenosis of liver and muscle due to phosphorylase
            kinase deficiency is caused by mutations in the phosphorylase
            kinase beta subunit (PHKB)
  JOURNAL   Hum Mol Genet 6 (7), 1109-1115 (1997)
   PUBMED   9215682
REFERENCE   8  (bases 1 to 5459)
  AUTHORS   Wullrich-Schmoll,A. and Kilimann,M.W.
  TITLE     Structure of the human gene encoding the phosphorylase kinase beta
            subunit (PHKB)
  JOURNAL   Eur J Biochem 238 (2), 374-380 (1996)
   PUBMED   8681948
REFERENCE   9  (bases 1 to 5459)
  AUTHORS   Herbert,M., Goldstein,J.L., Rehder,C., Austin,S., Kishnani,P.S. and
            Bali,D.S.
  TITLE     Phosphorylase Kinase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21634085
REFERENCE   10 (bases 1 to 5459)
  AUTHORS   Francke,U., Darras,B.T., Zander,N.F. and Kilimann,M.W.
  TITLE     Assignment of human genes for phosphorylase kinase subunits alpha
            (PHKA) to Xq12-q13 and beta (PHKB) to 16q12-q13
  JOURNAL   Am J Hum Genet 45 (2), 276-282 (1989)
   PUBMED   2757032
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X84908.1 and AC092374.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000293.2.
            
            Summary: Phosphorylase kinase is a polymer of 16 subunits, four
            each of alpha, beta, gamma and delta. The alpha subunit includes
            the skeletal muscle and hepatic isoforms, encoded by two different
            genes. The beta subunit is the same in both the muscle and hepatic
            isoforms, encoded by this gene, which is a member of the
            phosphorylase b kinase regulatory subunit family. The gamma subunit
            also includes the skeletal muscle and hepatic isoforms, encoded by
            two different genes. The delta subunit is a calmodulin and can be
            encoded by three different genes. The gamma subunits contain the
            active site of the enzyme, whereas the alpha and beta subunits have
            regulatory functions controlled by phosphorylation. The delta
            subunit mediates the dependence of the enzyme on calcium
            concentration. Mutations in this gene cause glycogen storage
            disease type 9B, also known as phosphorylase kinase deficiency of
            liver and muscle. Alternatively spliced transcript variants
            encoding different isoforms have been identified in this gene. Two
            pseudogenes have been found on chromosomes 14 and 20,
            respectively.[provided by RefSeq, Feb 2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X84908.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2146411, SAMEA2151741
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000323584.10/ ENSP00000313504.5
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4164              X84908.1           5-4168
            4165-5459           AC092374.4         10246-11540         c
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q12.1"
     gene            1..5459
                     /gene="PHKB"
                     /note="phosphorylase kinase regulatory subunit beta"
                     /db_xref="GeneID:5257"
                     /db_xref="HGNC:HGNC:8927"
                     /db_xref="MIM:172490"
     exon            1..96
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..3302
                     /gene="PHKB"
                     /EC_number="2.7.11.19"
                     /note="isoform a is encoded by transcript variant 1;
                     phosphorylase b kinase regulatory subunit beta;
                     phosphorylase kinase beta-subunit; phosphorylase kinase
                     subunit beta; phosphorylase kinase, beta"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit beta
                     isoform a"
                     /protein_id="NP_000284.1"
                     /db_xref="CCDS:CCDS10729.1"
                     /db_xref="GeneID:5257"
                     /db_xref="HGNC:HGNC:8927"
                     /db_xref="MIM:172490"
                     /translation="MAGAAGLTAEVSWKVLERRARTKRSGSVYEPLKSINLPRPDNET
                     LWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCGGDQKAKIQDSLYCAAGAWALALAYR
                     RIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTCLHSVFNVHTGDE
                     LLSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDF
                     GVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNR
                     QTLCSLLPRESRSHNTDAALLPCISYPAFALDDEVLFSQTLDKVVRKLKGKYGFKRFL
                     RDGYRTSLEDPNRCYYKPAEIKLFDGIECEFPIFFLYMMIDGVFRGNPKQVQEYQDLL
                     TPVLHHTTEGYPVVPKYYYVPADFVEYEKNNPGSQKRFPSNCGRDGKLFLWGQALYII
                     AKLLADELISPKDIDPVQRYVPLKDQRNVSMRFSNQGPLENDLVVHVALIAESQRLQV
                     FLNTYGIQTQTPQQVEPIQIWPQQELVKAYLQLGINEKLGLSGRPDRPIGCLGTSKIY
                     RILGKTVVCYPIIFDLSDFYMSQDVFLLIDDIKNALQFIKQYWKMHGRPLFLVLIRED
                     NIRGSRFNPILDMLAALKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEF
                     KSFEELEPPKHSKVKRQSSTPSAPELGQQPDVNISEWKDKPTHEILQKLNDCSCLASQ
                     AILLGILLKREGPNFITKEGTVSDHIERVYRRAGSQKLWLAVRYGAAFTQKFSSSIAP
                     HITTFLVHGKQVTLGAFGHEEEVISNPLSPRVIQNIIYYKCNTHDEREAVIQQELVIH
                     IGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPALDLYQLSPSEVKQLLLDI
                     LQPQQNGRCWLNRRQIDGSLNRTPTGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDM
                     TMYEMNFSLLVEDTLGNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKE
                     AFNEFQKDQSRLKEIEKQDDMTSFYNTPPLGKRGTCSYLTKAVMNLLLEGEVKPNNDD
                     PCLIS"
     exon            97..186
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            187..325
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            326..425
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            426..533
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            534..614
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            615..730
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            731..794
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            795..890
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1088
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1146
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1224
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1383
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1478
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1534
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1628
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1712
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1817
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1900
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..1991
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2053
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2216
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2298
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2356
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..2447
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2650
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2651..2785
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2915
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            2916..3023
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            3024..3164
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..5459
                     /gene="PHKB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3661..3666
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKB"
                     /note="hexamer: AATAAA"
     polyA_site      3677
                     /gene="PHKB"
     regulatory      3915..3920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKB"
                     /note="hexamer: AATAAA"
     polyA_site      3938
                     /gene="PHKB"
                     /note="major polyA site"
     regulatory      4376..4381
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKB"
                     /note="hexamer: ATTAAA"
     polyA_site      4398
                     /gene="PHKB"
     regulatory      5436..5441
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKB"
                     /note="hexamer: AGTAAA"
     polyA_site      5459
                     /gene="PHKB"
ORIGIN      
        1 aaggcggcga ccggagcgcg atggcggggg cggcgggact cacggcagaa gtgagctgga
       61 aggtcttgga gcgaagagct cggaccaagc gctcaggctc agtttatgaa cctcttaaaa
      121 gcattaatct tccaagacct gataatgaaa ctctctggga taagttggac cattattaca
      181 gaattgtcaa gtcaacattg ctgctgtatc aaagtccaac taccggtctc tttcccacta
      241 aaacatgcgg tggtgaccag aaggccaaga tccaggacag cctatactgc gctgctgggg
      301 cctgggcttt ggctcttgca tacaggcgaa ttgatgatga caagggaagg acccatgagc
      361 tggagcactc agctataaaa tgcatgagag gaattctcta ctgctatatg cgtcaggccg
      421 ataaggtcca gcagtttaag caggatccac gcccaacaac atgtcttcac tctgttttca
      481 atgtgcatac aggagatgag ttgctttcct atgaggaata tggtcatctt cagataaatg
      541 cagtgtcact ttatctcctt taccttgtgg aaatgatttc ctcaggactc cagattatct
      601 acaacactga tgaggtctct tttattcaaa accttgtatt ttgtgtggaa agagtttacc
      661 gtgtgcctga ctttggtgtc tgggaaagag gaagcaaata taataatggc agcacagagc
      721 tacattcgag ctcggttggt ttagcaaaag cagctctaga agcaattaat ggattcaacc
      781 tttttggcaa ccagggctgt tcgtggtcag ttatatttgt ggatctcgat gctcacaatc
      841 gcaacaggca aactttgtgc tcgctgttac ccagagaatc aagatcacat aatacagatg
      901 ctgccctgct cccctgcatc agttatcctg catttgccct ggatgatgaa gttcttttta
      961 gccagacact tgataaagtg gttagaaaat taaaaggaaa atatggattt aaacgtttct
     1021 tgagagatgg gtatagaaca tcattggaag atcccaacag atgctactac aagccagctg
     1081 aaattaagct atttgatggc attgaatgtg aatttcccat atttttcctt tatatgatga
     1141 ttgatggagt ttttagaggc aatcctaagc aagtacagga atatcaggat cttttgactc
     1201 cagtacttca tcataccaca gaaggatatc ctgttgtacc aaagtactat tatgtgccag
     1261 ctgactttgt agaatatgaa aaaaataacc ctggtagtca aaaacgattt cctagcaact
     1321 gtggccgtga tggaaaactg tttctttggg gacaagcact ttatatcatc gcaaaactcc
     1381 tggctgatga acttattagt cctaaagaca ttgatcctgt ccagcgctat gtcccactaa
     1441 aggatcaacg taacgtgagc atgaggtttt ccaatcaggg cccactggaa aatgacttgg
     1501 tagttcatgt ggcacttata gcagaaagcc aaagacttca agtttttctg aacacatatg
     1561 gtattcaaac tcaaactcct caacaagtag aacccattca gatatggcct cagcaggagc
     1621 ttgtgaaagc ttatttgcag ctgggtatca atgaaaagtt aggactctct ggaaggccag
     1681 acaggcccat tggctgcctc gggacatcaa agatttatcg cattctagga aagactgtgg
     1741 tttgttaccc gattattttc gacctaagtg atttctacat gtctcaggat gttttcctgc
     1801 tgatagatga cataaagaat gcgctgcagt tcattaaaca atattggaaa atgcatggac
     1861 gtccactttt ccttgttctc atccgggaag acaatataag aggtagccgg ttcaacccca
     1921 tattagatat gctggcagcc cttaaaaaag gaataattgg aggagtcaaa gttcatgtgg
     1981 atcgtctaca gacactaata tctggagctg tggtagaaca acttgatttc ctacgaatca
     2041 gtgacacaga agagcttcca gaatttaaga gttttgagga actagaacct cccaaacatt
     2101 caaaagtcaa acggcaaagc agcaccccta gtgctcctga actgggacag cagccggatg
     2161 tcaacattag tgaatggaag gacaaaccca cccacgaaat tcttcaaaaa ctgaatgatt
     2221 gcagttgtct ggctagccaa gccatcctgc tgggtatact gctcaaaaga gaaggcccca
     2281 acttcatcac aaaggaaggt accgtttctg atcacattga gagagtctat agaagagctg
     2341 gcagccaaaa actttggttg gcggtgcgct acggggctgc atttacccag aaattttctt
     2401 cctctatagc cccacacatt actacttttc tggtacatgg gaaacaggta actctgggtg
     2461 cctttgggca tgaagaagaa gttatctcta atcctttgtc tccaagagtg attcaaaaca
     2521 tcatctatta taagtgtaac acccatgatg agagggaagc ggtcattcag caagaactgg
     2581 tcatccatat tggctggatc atctccaata accctgagtt attcagtggc atgctgaaaa
     2641 tacgaatcgg gtggatcatc catgccatgg agtatgaact tcagatccgt ggcggagaca
     2701 agccagcctt ggacttgtat cagctgtcac ctagtgaagt taaacagctt ctgctggata
     2761 ttctgcagcc tcaacagaat ggaagatgtt ggctgaacag gcgtcagatc gatgggtctt
     2821 tgaatagaac tcccaccggg ttctatgacc gagtgtggca gattctggag cgcacgccca
     2881 atgggatcat tgttgctggg aagcatttgc ctcagcaacc aaccctgtca gatatgacca
     2941 tgtatgagat gaatttctct ctccttgttg aagacacgtt gggaaatatt gaccagccac
     3001 agtacagaca gatcgttgta gagttactta tggttgtatc cattgtactg gaaagaaacc
     3061 ccgagctaga atttcaagac aaagtagatc tagacagact ggtcaaagaa gcatttaatg
     3121 aatttcaaaa agatcagagt cggctaaagg aaattgaaaa acaagatgac atgacttcct
     3181 tttacaacac tcctcccctg ggaaaaagag gaacatgcag ctatttgaca aaggcggtga
     3241 tgaatctgct gctggaagga gaagtcaagc caaacaatga tgacccgtgt ctgattagct
     3301 agtggggaag gtgtaggaag ctctgttgag acacatgttc tgaagtgtgt tgtgtttcat
     3361 gttcaagctt aatcaaggca gccattaata tacgaactga gcatgctggg gaggtgaatg
     3421 ccacatcctt ggcggggtta tggacctctt gcatgtcata gccaatctaa cggtaatggt
     3481 aaatgctttt aatcaagcag gaaaaagttc tcatgattat gccaactata atagtaatcc
     3541 tcactgagtg ataaaaatag tttatgaatt gaaaatttgc cgctgcatgt tgtatgatca
     3601 aatagttcat caaaatgaat ctttgctctt tggactgaat tcttaccata ctgccattaa
     3661 aataaatttg ccaactagta atgcatactg gaaatcaaaa gatactgaaa gaatggtgaa
     3721 cttctcttag tggtattgtc atgctaaaag atgttaatat acatcataaa agcaaagtca
     3781 gccagctgat attttggttc tcaaaaactg cattattaat aatattttag tatacagagc
     3841 tattctacag tttttacatt gtaaacatga ctgtggtttt gtatttgcta aatatagggg
     3901 ttggactaaa atataataaa tctgtacctt atcaaacatt ttctttgagc tcctgctaaa
     3961 aataggacat gtctatgatt gttcaaaaat atgttaaatt taggctcagc acagtagctc
     4021 acacctgaaa tcttagcact tcgggaggct gaggcaggtg gatcacttga ggttaggagt
     4081 tcaagaccag cccagccaac atggtgaaaa ccctgtctct actaaaaata caaaaattag
     4141 ccaggcatga tggtgcatgc ctttaaaccc agctactgag gaggctgagg catgagaatt
     4201 gcttgaacca ggagacggag gttgcagtga gctgaaatcc tgccactgca caccagcctg
     4261 ggtgacagag cgagactcca tctcaaaaaa aaaaaaaaaa aagaaaaaga aaaaaatatg
     4321 ttaaatttaa ggactgttaa cctacgttct actagtaaaa acaacattaa gggtcattaa
     4381 attttatttc tgaattaatg aacatatctg gacatttttt gttcctaggt tcacaagatt
     4441 gagcttcaaa ctcccgagtg ttttcattta attgatttag aggtagaatg gaagagaatc
     4501 tgtggtacct accattatac acatttagtt gcccagttta cttaaacttt aatgaaagta
     4561 gaaagtagat taaacattta aattttattt ctttcctaaa tagaaaaaaa aaaggcccat
     4621 gagtttgagc ccttgcccca aagagagttg tttttctgat caatcttact ggagtttcac
     4681 ctgagaagat agatttgtca cataaaaaat gcagcttatt tgtagtgttt tgaaaggatg
     4741 taagaaagga tgagtggctt cagtattgag tcaatcgata tcaaatgaat ggatgtgtga
     4801 gcacatggaa aaatctgctg tcagtcacat ttctcataac attgaggtac agtgccgaga
     4861 tcttgcataa gaaattgtat ggatattagt tggcaaaatt gaaatgaatg agacacagtt
     4921 gggccctaaa gaactaagga gggggattga caaagaatag aattaaacta cttgactcaa
     4981 tatgttaaga gagtttatga aaagatagtc gtgaagggtg aagctaattt gtaaaactaa
     5041 tgcctgaaaa aaacgtaagc atgaagtcca tggagaggga attgagagct gacacttcat
     5101 aactggagaa actggaaatt aaagaaaggg aaacaaaaga cttaaaattt ttgtttgccg
     5161 aaatttttac gaaagtaaac tgtgttgtag ttttaagaat ttttttaaaa aatctgatta
     5221 gctgagcaaa gttaccagaa aactgctatc ctagttgaaa tcaaacatta cttgcaaatg
     5281 tgttttctga gtgttagctt cctgcttaaa tgttacatat gcaaaattct gaagttttcc
     5341 tgtgctactc ctgaaagcat tcagaatctg tgacacgtat tgacttcatg ctttgagatt
     5401 caaaatttat tgttctgaaa gttatattta gaatcagtaa atcttgatat tcaccttta
//
