LOCUS       NM_000292               5077 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens phosphorylase kinase regulatory subunit alpha 2
            (PHKA2), mRNA.
ACCESSION   NM_000292
VERSION     NM_000292.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Dong,R., Wei,X., Zhang,K., Song,F., Lv,Y., Gao,M., Wang,D., Ma,J.,
            Gai,Z. and Liu,Y.
  TITLE     Genotypic and phenotypic characteristics of 12 chinese children
            with glycogen storage diseases
  JOURNAL   Front Genet 13, 932760 (2022)
   PUBMED   36105079
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 5077)
  AUTHORS   Benner,A., Alhaidan,Y., Lines,M.A., Brusgaard,K., De Leon,D.D.,
            Sparkes,R., Frederiksen,A.L. and Christesen,H.T.
  TITLE     PHKA2 variants expand the phenotype of phosphorylase B kinase
            deficiency to include patients with ketotic hypoglycemia only
  JOURNAL   Am J Med Genet A 185 (10), 2959-2975 (2021)
   PUBMED   34117828
  REMARK    GeneRIF: PHKA2 variants expand the phenotype of phosphorylase B
            kinase deficiency to include patients with ketotic hypoglycemia
            only.
REFERENCE   4  (bases 1 to 5077)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5077)
  AUTHORS   Fernandes,S.A., Cooper,G.E., Gibson,R.A. and Kishnani,P.S.
  TITLE     Benign or not benign? Deep phenotyping of liver Glycogen Storage
            Disease IX
  JOURNAL   Mol Genet Metab 131 (3), 299-305 (2020)
   PUBMED   33317799
  REMARK    GeneRIF: Benign or not benign? Deep phenotyping of liver Glycogen
            Storage Disease IX.
REFERENCE   6  (bases 1 to 5077)
  AUTHORS   Herbert,M., Goldstein,J.L., Rehder,C., Austin,S., Kishnani,P.S. and
            Bali,D.S.
  TITLE     Phosphorylase Kinase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21634085
REFERENCE   7  (bases 1 to 5077)
  AUTHORS   Davidson,J.J., Ozcelik,T., Hamacher,C., Willems,P.J., Francke,U.
            and Kilimann,M.W.
  TITLE     cDNA cloning of a liver isoform of the phosphorylase kinase alpha
            subunit and mapping of the gene to Xp22.2-p22.1, the region of
            human X-linked liver glycogenosis
  JOURNAL   Proc Natl Acad Sci U S A 89 (6), 2096-2100 (1992)
   PUBMED   1372435
REFERENCE   8  (bases 1 to 5077)
  AUTHORS   Wauters,J.G., Bossuyt,P.J., Davidson,J., Hendrickx,J.,
            Kilimann,M.W. and Willems,P.J.
  TITLE     Regional mapping of a liver alpha-subunit gene of phosphorylase
            kinase (PHKA) to the distal region of human chromosome Xp
  JOURNAL   Cytogenet Cell Genet 60 (3-4), 194-196 (1992)
   PUBMED   1505214
REFERENCE   9  (bases 1 to 5077)
  AUTHORS   Willems,P.
  TITLE     Families with X-linked liver glycogenosis due to phosphorylase
            kinase deficiency
  JOURNAL   Clin Genet 38 (1), 80 (1990)
   PUBMED   2387090
REFERENCE   10 (bases 1 to 5077)
  AUTHORS   Willems,P.J., Gerver,W.J., Berger,R. and Fernandes,J.
  TITLE     The natural history of liver glycogenosis due to phosphorylase
            kinase deficiency: a longitudinal study of 41 patients
  JOURNAL   Eur J Pediatr 149 (4), 268-271 (1990)
   PUBMED   2303074
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC014036.2, X80497.1,
            CD722555.1 and W63591.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_000292.2.
            
            Summary: Phosphorylase kinase is a polymer of 16 subunits, four
            each of alpha, beta, gamma and delta. The alpha subunit includes
            the skeletal muscle and hepatic isoforms, and the hepatic isoform
            is encoded by this gene. The beta subunit is the same in both the
            muscle and hepatic isoforms, and encoded by one gene. The gamma
            subunit also includes the skeletal muscle and hepatic isoforms,
            which are encoded by two different genes. The delta subunit is a
            calmodulin and can be encoded by three different genes. The gamma
            subunits contain the active site of the enzyme, whereas the alpha
            and beta subunits have regulatory functions controlled by
            phosphorylation. The delta subunit mediates the dependence of the
            enzyme on calcium concentration. Mutations in this gene cause
            glycogen storage disease type 9A, also known as X-linked liver
            glycogenosis. Alternatively spliced transcript variants have been
            reported, but the full-length nature of these variants has not been
            determined.[provided by RefSeq, Feb 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.260408.1, D38616.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379942.5/ ENSP00000369274.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1760              BC014036.2         36-1795
            1761-4411           X80497.1           1705-4355
            4412-4734           CD722555.1         298-620
            4735-5077           W63591.1           139-481
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.13"
     gene            1..5077
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /note="phosphorylase kinase regulatory subunit alpha 2"
                     /db_xref="GeneID:5256"
                     /db_xref="HGNC:HGNC:8926"
                     /db_xref="MIM:300798"
     exon            1..260
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..3890
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /EC_number="2.7.11.19"
                     /note="phosphorylase kinase alpha L subunit; phosphorylase
                     kinase alpha-subunit"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit alpha,
                     liver isoform"
                     /protein_id="NP_000283.1"
                     /db_xref="CCDS:CCDS14190.1"
                     /db_xref="GeneID:5256"
                     /db_xref="HGNC:HGNC:8926"
                     /db_xref="MIM:300798"
                     /translation="MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHEQKDAWV
                     RDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVAKVEKF
                     KHTQSTKDSLHAKYNTATCGTVVGDDQWGHLQVDATSLFLLFLAQMTASGLRIIFTLD
                     EVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLF
                     GAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLV
                     NVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTY
                     FIIDGVFSGDAVQVQEYREALEGILIRGKNGIRLVPELYAVPPNKVDEEYKNPHTVDR
                     VPMGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNH
                     IKDLLRKHGVNVQSIADIHPIQVQPGRILSHIYAKLGRNKNMNLSGRPYRHIGVLGTS
                     KLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPI
                     SRTMLTNDGSDIHSAVLSTIRKLEDGYFGGARVKLGNLSEFLTTSFYTYLTFLDPDCD
                     EKLFDNASEGTFSPDSDSDLVGYLEDTCNQESQDELDHYINHLLQSTSLRSYLPPLCK
                     NTEDRHVFSAIHSTRDILSVMAKAKGLEVPFVPMTLPTKVLSAHRKSLNLVDSPQPLL
                     EKVPESDFQWPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQ
                     HGVTVQNLLGELYGKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLP
                     PEPREKIISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLR
                     LRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSVRP
                     IHSSTSSPTISIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQFFSVGQAASSS
                     AHSSKSARSSTPSSPTGTSSSDSGGHHIGWGERQGQWLRRRRLDGAINRVPVGFYQRV
                     WKILQKCHGLSIDGYVLPSSTTREMTPHEIKFAVHVESVLNRVPQPEYRQLLVEAIMV
                     LTLLSDTEMTSIGGIIHVDQIVQMASQLFLQDQVSIGAMDTLEKDQATGICHFFYDSA
                     PSGAYGTMTYLTRAVASYLQELLPNSGCQMQ"
     exon            261..419
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            420..467
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            468..636
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            637..719
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            720..800
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            801..899
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1046
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1100
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1223
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1319
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1427
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1506
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..1641
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1751
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1896
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..1975
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2145
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2319
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2408
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2542
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2543..2699
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2700..2779
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2780..2858
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..2988
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3090
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3091..3209
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3210..3239
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3240..3293
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3294..3464
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3518
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3519..3719
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     exon            3720..5077
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4378..4383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /note="hexamer: AATAGA"
     polyA_site      4405
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /note="major polyA site"
     regulatory      5052..5057
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
                     /note="hexamer: AATAAA"
     polyA_site      5077
                     /gene="PHKA2"
                     /gene_synonym="GSD9A; PHK; PYK; PYKL; XLG; XLG2"
ORIGIN      
        1 gctttccaac cggactttgg ggctagcgtt tagaaaagtc tcgacctcct ccggcccggt
       61 cccatcccaa gaaccgacta aggctgtgag tgtccgggaa ccagacccgc ttggaggcca
      121 cagccccgac gtcccgcgcc cacgcggcag atcgggcgct gcggcctggg agcctcgggg
      181 agatgcggag caggagcaat tccggggtcc gcttggacgg gtacgcgcgg ctggtgcagc
      241 aaaccatcct gtgttaccag aatcccgtca cggggctgct gtcagccagc catgagcaga
      301 aggatgcctg ggtgcgggat aacatctaca gtatcctggc cgtgtggggc ctgggcatgg
      361 cctaccgtaa gaatgcagac cgcgatgagg acaaggccaa ggcctacgag ctggagcaga
      421 acgtggtgaa gctgatgcga ggtcttctcc agtgcatgat gagacaggtg gccaaagtgg
      481 agaagttcaa acacactcag agcaccaagg acagcctgca cgccaagtac aacaccgcca
      541 cctgtggcac ggtggtgggc gacgaccagt ggggccacct ccaggtggat gccacctctc
      601 tcttcctcct gttcctggcc cagatgaccg cctcaggctt acgtatcatt ttcactctcg
      661 atgaggtggc cttcatacag aatcttgtct tttacataga agctgcatat aaagtcgctg
      721 attatggaat gtgggagcgt ggagataaga ctaatcaggg catcccggaa ttgaatgcaa
      781 gctccgtagg aatggccaag gcagctcttg aggcaattga tgaactggac ctttttggag
      841 cccatggagg acgcaagtca gtgattcatg ttctgccaga tgaggtcgag cactgccagt
      901 ctattctgtt ctccatgctg ccaagagcgt cgacatctaa agaaattgat gctggacttc
      961 tttccattat ttccttcccg gcctttgcag tggaagatgt aaaccttgta aatgtgacca
     1021 aaaatgaaat tatttctaag ctccaggggc gttatggatg ctgtcgcttc cttcgagatg
     1081 gttataaaac tccaagagag gaccctaatc gactgcatta tgaccctgct gaactcaagc
     1141 tcttcgaaaa cattgaatgt gagtggcctg tgttttggac atattttata atagatggag
     1201 tcttcagtgg tgatgctgtt caggtccaag aataccgaga ggccctggag ggaatactca
     1261 tcagaggcaa gaatgggatc cgcctggtgc ctgaactcta cgctgtcccg cctaacaagg
     1321 tagatgaaga gtacaagaat cctcacacag tagaccgagt tcctatgggg aaggtgcctc
     1381 atctgtgggg ccaatccttg tacatcctca gctcgctgtt ggcagaggga ttccttgccg
     1441 ctggtgaaat cgatccctta aatagaagat tttccacttc agtcaaacct gatgttgtag
     1501 tacaagttac tgttttggca gaaaacaatc acattaagga cttattgagg aaacacgggg
     1561 tgaacgtcca gagtatcgcg gacattcatc caattcaagt ccagccgggc cggattctta
     1621 gtcacatata tgccaagctt ggacggaata agaatatgaa tttgagtggg cgaccgtatc
     1681 gacatattgg tgtccttgga acctctaaac tatatgtgat taggaaccaa atctttactt
     1741 ttacacccca gttcaccgac cagcatcact tctacctggc cctcgacaat gagatgatcg
     1801 tggagatgct aaggatcgag ctggcctacc tgtgcacctg ctggaggatg acgggcagac
     1861 ccacactcac cttccccatc agtcgcacca tgctcacaaa tgatggctca gacattcatt
     1921 ctgctgtgct ctccacaatt agaaaactag aggatggata ttttggagga gccagagtaa
     1981 aattagggaa cctttcggaa tttctcacca catcgttcta cacatatctg acttttctgg
     2041 atccagactg tgatgagaag ttgtttgaca atgccagcga agggactttc agtcctgata
     2101 gtgattcaga tttggtagga tatctggaag acacctgtaa tcaagaaagc caagacgaac
     2161 ttgaccatta tatcaaccac cttctgcaaa gcacatcgtt gaggtcctat ctgcctcctc
     2221 tttgtaagaa cacagaagac cgccatgtct tcagtgctat ccactccacg cgggacatac
     2281 tttctgtgat ggcaaaagca aagggtttgg aagttccatt tgttcccatg actttgccga
     2341 ctaaagttct aagtgcccac cgtaaatcac tgaatcttgt tgattctcct cagccactcc
     2401 tagaaaaggt tcctgaaagt gactttcagt ggcccagaga tgaccatggt gacgtggact
     2461 gtgagaagct ggttgagcag ctaaaagatt gttcgaacct acaggaccaa gcagacattc
     2521 tgtacattct ttatgtcata aagggtccca gctgggacac aaatctctct ggacagcacg
     2581 gggtcaccgt tcaaaacctt cttggtgagc tctatgggaa agccggcttg aaccaggagt
     2641 ggggtctgat tcgctacatc tcaggccttc tcaggaagaa agtggaggtc ctggctgagg
     2701 cctgcacaga cctgctttcg caccagaagc agctcaccgt gggcctgccg cccgagcccc
     2761 gggagaagat catctctgcg ccccttcccc cagaggagct cacaaaactc atctacgagg
     2821 ccagtgggca ggacatcagc attgccgtcc tcacgcagga gattgtggtt tacctggcca
     2881 tgtatgtcag ggcgcagccc agcctctttg tggagatgct gagactccgg attggactga
     2941 tcattcaggt gatggccacg gagctggcac ggagcctgaa ctgctcagga gaagaggctt
     3001 ctgaaagttt gatgaacctc agccctttcg atatgaaaaa tctcctgcac catattctaa
     3061 gtgggaaaga gtttggcgtt gaaagaagtg tgcgccctat ccactcctcc acatccagcc
     3121 ctaccatctc catccacgag gtgggccata ccggagtcac caaaactgag aggagtggca
     3181 ttaacagact gaggagtgaa atgaaacaga tgactaggcg gtttagtgct gatgaacagt
     3241 tcttttctgt gggccaggcc gcgtccagca gtgcgcattc ctccaagtct gcgaggtcca
     3301 gcaccccatc ctcgcccact ggcacgtcat cctcagactc gggaggacat cacatcggct
     3361 ggggtgagcg gcagggccag tggctgcgca ggagaaggct ggatggggcc atcaacaggg
     3421 tccccgtggg attctaccag agggtgtgga agatcctcca gaagtgccac ggtctctcca
     3481 tcgatggtta tgtcctccca tcctcgacga cccgagagat gaccccgcat gagatcaagt
     3541 ttgctgtcca tgtcgaatcg gtgctgaacc gcgtgccgca gcccgagtac cggcagctgc
     3601 tggtggaagc catcatggtg ctgacgctgc tctcggacac ggagatgacc agcatcgggg
     3661 gcatcatcca cgtggaccag atcgtgcaga tggccagtca gctgttcttg caggaccagg
     3721 tgtcaattgg tgccatggac accctggaga aagaccaagc cacaggaatc tgccacttct
     3781 tttatgacag cgctccgagt ggggcttatg ggacgatgac ctacctaaca agagcagtgg
     3841 cttcttattt gcaggaattg ttgcccaatt cgggctgcca gatgcaatag ggtctcacct
     3901 ggaaacatga tcacactctc aatctgtcac gtgcccccta gccttactgg gaaccttctg
     3961 tcccccaaga tcccctgtgc tatcaggaaa gcatgtccca tcagaaacac tctcgggggg
     4021 caatggtagc actcaccctg aaactgatgt atgttaaagc cacagagata gagctgagga
     4081 gtcctgtgtt cccccgcaag gagcaccccg ggatcatttt ctaggttcat ttctctggaa
     4141 catttgctgt agcatctggt ctcacggact ctgaggagga attggaaatt ggtctctttt
     4201 gagtgcagag ggaactgaga cgccagctta aattggctct tgcagagagt tacagaaata
     4261 gtttcgatga gctagtgaca catcctaaag atgcaaagat cctcctggcg gcagtagcct
     4321 tgacaagggc cacctcttca caggatgcag tctgtctgtg caccaaactc ttcaccaaat
     4381 agaacacttg tgtctctctg tggaatgggg gttttcttgt gccttgcttg ctttcatagc
     4441 cttccatttt attggcatgg ctgccttgat gtaacataat tctctgtccc caagatttag
     4501 aaaattcctc ttcgttcacc ttggctcatg gtcttccagg gtttttatcc tggctgtcta
     4561 tgaactaggg ttttctcctg ccttaggaaa aatactgcat cttctggaat ctagaaaaaa
     4621 aaaaaaaaaa agacaaggct cctcttatag ccaggcaagc agttgtttag ggctagacct
     4681 gttgtccccg ctcgcgtggg gtgaacgccc cgcagtggat actgtcctcc tgtgctctgg
     4741 gcgaggcgtc atagggtgag cacaagtcag cagtgccatg gaagtgaaca ggcatgtccc
     4801 cgcagaaaca atgcttccct ctgaaaaata gagccaggct aggaacctgt accctcttgg
     4861 tagaagcact ttggtcttca ctctcttggg tgggtgcagt tggaggcacc gattggagga
     4921 gagcaaggtt gagtcaggtg tcagggtctg gcaaatcttt tgccaatgcc ctagcaatgg
     4981 agtttctctc cgggcattgt cttaccttgg gggaaggaaa caaaaccaat gggaaaacag
     5041 tttctgtaaa taataaacct tgaaagacaa atgctca
//
