LOCUS       NM_002637               6286 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens phosphorylase kinase regulatory subunit alpha 1
            (PHKA1), transcript variant 1, mRNA.
ACCESSION   NM_002637
VERSION     NM_002637.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Bisciglia,M., Froissart,R., Bedat-Millet,A.L., Romero,N.B.,
            Pettazzoni,M., Hogrel,J.Y., Petit,F.M. and Stojkovic,T.
  TITLE     A novel PHKA1 mutation associating myopathy and cognitive
            impairment: Expanding the spectrum of phosphorylase kinase b (PhK)
            deficiency
  JOURNAL   J Neurol Sci 424, 117391 (2021)
   PUBMED   33799212
  REMARK    GeneRIF: A novel PHKA1 mutation associating myopathy and cognitive
            impairment: Expanding the spectrum of phosphorylase kinase b (PhK)
            deficiency.
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6286)
  AUTHORS   Preisler,N., Orngreen,M.C., Echaniz-Laguna,A., Laforet,P.,
            Lonsdorfer-Wolf,E., Doutreleau,S., Geny,B., Akman,H.O., Dimauro,S.
            and Vissing,J.
  TITLE     Muscle phosphorylase kinase deficiency: a neutral metabolic variant
            or a disease?
  JOURNAL   Neurology 78 (4), 265-268 (2012)
   PUBMED   22238410
  REMARK    GeneRIF: muscle PHKA deficiency may present as an almost
            asymptomatic condition, despite a mild impairment of muscle
REFERENCE   4  (bases 1 to 6286)
  AUTHORS   Echaniz-Laguna,A., Akman,H.O., Mohr,M., Tranchant,C.,
            Talmant-Verbist,V., Rolland,M.O. and Dimauro,S.
  TITLE     Muscle phosphorylase b kinase deficiency revisited
  JOURNAL   Neuromuscul Disord 20 (2), 125-127 (2010)
   PUBMED   20080404
REFERENCE   5  (bases 1 to 6286)
  AUTHORS   Orngreen,M.C., Schelhaas,H.J., Jeppesen,T.D., Akman,H.O.,
            Wevers,R.A., Andersen,S.T., ter Laak,H.J., van Diggelen,O.P.,
            DiMauro,S. and Vissing,J.
  TITLE     Is muscle glycogenolysis impaired in X-linked phosphorylase b
            kinase deficiency?
  JOURNAL   Neurology 70 (20), 1876-1882 (2008)
   PUBMED   18401027
  REMARK    GeneRIF: X-linked PHK deficiency causes a mild metabolic myopathy
            with blunted muscle glycogen breakdown and impaired lactate
            production during dynamic exercise, which impairs oxidative
            capacity only marginally
REFERENCE   6  (bases 1 to 6286)
  AUTHORS   Gossen,M., Wullrich,A. and Kilimann,M.W.
  TITLE     Dinucleotide repeat polymorphism within the PHKA1 gene at Xq12-q13
  JOURNAL   Hum Genet 95 (4), 469-470 (1995)
   PUBMED   7705849
REFERENCE   7  (bases 1 to 6286)
  AUTHORS   Wehner,M., Clemens,P.R., Engel,A.G. and Kilimann,M.W.
  TITLE     Human muscle glycogenosis due to phosphorylase kinase deficiency
            associated with a nonsense mutation in the muscle isoform of the
            alpha subunit
  JOURNAL   Hum Mol Genet 3 (11), 1983-1987 (1994)
   PUBMED   7874115
REFERENCE   8  (bases 1 to 6286)
  AUTHORS   Herbert,M., Goldstein,J.L., Rehder,C., Austin,S., Kishnani,P.S. and
            Bali,D.S.
  TITLE     Phosphorylase Kinase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21634085
REFERENCE   9  (bases 1 to 6286)
  AUTHORS   Meyer,H.E., Meyer,G.F., Dirks,H. and Heilmeyer,L.M. Jr.
  TITLE     Localization of phosphoserine residues in the alpha subunit of
            rabbit skeletal muscle phosphorylase kinase
  JOURNAL   Eur J Biochem 188 (2), 367-376 (1990)
   PUBMED   2108025
REFERENCE   10 (bases 1 to 6286)
  AUTHORS   Francke,U., Darras,B.T., Zander,N.F. and Kilimann,M.W.
  TITLE     Assignment of human genes for phosphorylase kinase subunits alpha
            (PHKA) to Xq12-q13 and beta (PHKB) to 16q12-q13
  JOURNAL   Am J Hum Genet 45 (2), 276-282 (1989)
   PUBMED   2757032
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC240504.3, DR002787.1,
            BC104944.1, X73874.1, BJ998054.1, BX647542.1 and AW450116.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002637.3.
            
            Summary: Phosphorylase kinase is a polymer of 16 subunits, four
            each of alpha, beta, gamma and delta. The alpha subunit includes
            the skeletal muscle and hepatic isoforms, and the skeletal muscle
            isoform is encoded by this gene. The beta subunit is the same in
            both the muscle and hepatic isoforms, and encoded by one gene. The
            gamma subunit also includes the skeletal muscle and hepatic
            isoforms, which are encoded by two different genes. The delta
            subunit is a calmodulin and can be encoded by three different
            genes. The gamma subunits contain the active site of the enzyme,
            whereas the alpha and beta subunits have regulatory functions
            controlled by phosphorylation. The delta subunit mediates the
            dependence of the enzyme on calcium concentration. Mutations in
            this gene cause glycogen storage disease type 9D, also known as
            X-linked muscle glycogenosis. Alternatively spliced transcript
            variants encoding different isoforms have been identified in this
            gene. A pseudogene has been found on chromosome 1.[provided by
            RefSeq, Feb 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X73874.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373542.9/ ENSP00000362643.4
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AC240504.3         157301-157425
            126-379             DR002787.1         22-275
            380-2128            BC104944.1         1-1749
            2129-4472           X73874.1           1864-4207
            4473-4528           BJ998054.1         75-130
            4529-6275           BX647542.1         1-1747
            6276-6286           AW450116.1         17-27               c
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.1"
     gene            1..6286
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="phosphorylase kinase regulatory subunit alpha 1"
                     /db_xref="GeneID:5255"
                     /db_xref="HGNC:HGNC:8925"
                     /db_xref="MIM:311870"
     exon            1..504
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     CDS             427..4098
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /EC_number="2.7.11.19"
                     /note="isoform 1 is encoded by transcript variant 1;
                     phosphorylase kinase, alpha 1 (muscle), muscle
                     glycogenosis; phosphorylase kinase alpha M subunit"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit alpha,
                     skeletal muscle isoform isoform 1"
                     /protein_id="NP_002628.2"
                     /db_xref="CCDS:CCDS14421.1"
                     /db_xref="GeneID:5255"
                     /db_xref="HGNC:HGNC:8925"
                     /db_xref="MIM:311870"
                     /translation="MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWV
                     RDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESF
                     KYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLD
                     EVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLF
                     GVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLV
                     ELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTY
                     FILDGVFSGNAEQVQEYKEALEAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDR
                     VPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEE
                     IKTILKDKGIYVETIAEVYPIRVQPARILSHIYSSLGCNNRMKLSGRPYRHMGVLGTS
                     KLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPI
                     SHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQTGKLSEFLTTSCCTHLSFMDPGPE
                     GKLYSEDYDDNYDYLESGNWMNDYDSTSHARCGDEVARYLDHLLAHTAPHPKLAPTSQ
                     KGGLDRFQAAVQTTCDLMSLVTKAKELHVQNVHMYLPTKLFQASRPSFNLLDSPHPRQ
                     ENQVPSVRVEIHLPRDQSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWNTEL
                     YNERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTV
                     GLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAE
                     MFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERS
                     VRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSISAESQSPGTSM
                     TPSSGSFPSAYDQQSSKDSRQGQWQRRRRLDGALNRVPVGFYQKVWKVLQKCHGLSVE
                     GFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIG
                     SIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLS
                     KAAATYVQEFLPHSICAMQ"
     exon            505..663
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            664..711
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            712..880
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            881..963
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1044
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1143
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1290
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1344
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1467
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1563
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1671
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1750
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1885
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1995
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2140
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2219
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2386
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2563
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..2655
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2795
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2796..2952
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2953..3032
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3033..3111
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3112..3241
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3343
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3459
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3498
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3499..3669
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3670..3723
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3724..3924
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..6286
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4453..4458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: AATAAT"
     polyA_site      4472
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="major polyA site"
     regulatory      4544..4549
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: AGTAAA"
     polyA_site      4566
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
     regulatory      6237..6242
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: AATAAG"
     regulatory      6251..6256
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: ATTAAA"
     polyA_site      6256
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
     polyA_site      6286
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
ORIGIN      
        1 gcctcccgct cgcgtctccc gcaaggtgga ggcggcgcag cggtcgttga cctgttggcg
       61 gcggccgggc gggaggctgt gggccgcggc ggtctcccgg ggttgagggg aggatgcagg
      121 agtcacacgc ggaggccgga ctggactaag ggggcaggag cggcgcctgg ggcgctgcgg
      181 cggggctcta gaccgcggga agccagtgct agcgctggcg cgtggtgggc ccctgaggcc
      241 gccggagtcc ggagaggtac gcggaatccg cggcgccagg cctgagcggt gggagggctc
      301 tgcggggcct ggtgttcagg cgtcccacca cgagggtgga gcagcgttgg atacttgttc
      361 cttagggacc gaaggctccg gtggcacccg ggctatttct cagaggacaa ttagtaacgt
      421 gtcgccatga ggagccggag taactccggg gtccggctgg acggctacgc tcgactggtg
      481 caacagacca tcctgtgcca tcagaatcca gtgactggct tgcttccagc cagctatgat
      541 cagaaagatg cttgggtccg agataatgtg tacagcatct tggctgtgtg gggtttgggc
      601 ctggcctatc ggaagaatgc agaccgggat gaggataagg caaaggccta tgaattggag
      661 cagagtgtag tgaagctgat gagaggacta ctgcactgca tgatcagaca ggtggataaa
      721 gtagaatcct tcaaatatag tcagagtact aaggatagcc tccatgcaaa gtacaacacc
      781 aaaacctgtg ccactgtagt gggtgatgat caatggggac acctgcagtt ggatgctacc
      841 tctgtgtacc tgctcttctt agcccaaatg actgcctcag gactccatat catccacagc
      901 ctagatgaag tcaatttcat acagaacctt gtgttttaca ttgaagctgc atataaaact
      961 gctgacttcg ggatatggga acgtggagac aagaccaacc aagggatctc agagttgaat
     1021 gccagttcag ttggaatggc aaaggcagcc ctggaagcat tagatgaact ggatctgttt
     1081 ggtgtgaaag gtgggcctca atcagttatc catgtcctgg ctgatgaagt acagcactgc
     1141 cagtctatcc taaattcact actgccccgt gcttcaacat caaaagaggt tgatgctagt
     1201 ctactctcag tggtttcctt ccctgccttt gcagtagagg atagccagtt ggtggagctc
     1261 acaaaacagg aaatcatcac caagcttcag ggtcgttatg gttgctgtcg ctttctacga
     1321 gatggatata aaactcctaa agaggatccc aatcgtctgt actatgaacc agctgagctg
     1381 aagctatttg aaaacattga gtgtgaatgg ccattgttct ggacatactt tattcttgat
     1441 ggggtcttca gtggcaatgc agaacaggtt caagaatata aagaggctct tgaagcagtc
     1501 ctcatcaagg gcaaaaatgg agtcccactt ctgccagagc tgtacagtgt tcctcctgac
     1561 agggtcgatg aagaatatca gaatcctcac actgtggacc gagtccccat ggggaaattg
     1621 cctcacatgt ggggtcagtc tctatacatt ttaggaagct tgatggcaga gggattttta
     1681 gcccctggag aaattgatcc cctgaatcgc aggttttcta ctgtaccgaa gcccgatgtt
     1741 gtggttcaag tctccattct agctgaaaca gaagaaatca agaccatttt gaaggacaag
     1801 ggaatttacg tggagaccat tgctgaggta taccccatca gagtacaacc agctcgtatt
     1861 ctcagccaca tttattccag cctaggatgc aacaatagaa tgaaactcag tggacgaccc
     1921 tacagacaca tgggagtgct tggaacttca aaactctatg acattcggaa aactatcttt
     1981 actttcactc cacagtttat agaccagcaa cagttctacc tggctctgga caacaagatg
     2041 atagtggaaa tgcttagaac agacctctcc tacctctgta gccgctggcg gatgacaggc
     2101 cagcccacca tcaccttccc catctcacac agcatgcttg atgaagatgg aacaagcttg
     2161 aattcaagta tcctggcagc actccgaaaa atgcaagatg ggtattttgg tggggcaagg
     2221 gttcaaacag gtaaattgtc agagtttttg acaacatctt gttgcacaca cttgagcttc
     2281 atggaccctg gacctgaggg taagctgtac agtgaagatt atgatgacaa ctatgattac
     2341 ctggaatctg gcaactggat gaatgattat gattcaacca gtcatgctcg ctgtggtgat
     2401 gaagttgctc gttatttaga tcaccttttg gcgcacactg ctccccatcc taaactagcc
     2461 cctacctcac agaagggagg gctagatcgg ttccaagctg ctgtgcaaac aacctgcgac
     2521 ttaatgtcct tggtgaccaa ggccaaggaa ctgcatgtac agaatgttca catgtatctt
     2581 cctacgaagt tatttcaggc ttcccggcct tcattcaact tacttgattc acctcatccc
     2641 cgacaggaga accaggttcc ctctgttcgt gtagaaatac atcttcctag agaccagtct
     2701 ggggaggtgg actttaaagc actggtttta cagttgaagg agacctcaag cttacaggaa
     2761 caagctgata tcctctatat gctgtatact atgaaaggac ctgactggaa cactgaattg
     2821 tataatgaac ggagtgctac agtgagagag cttcttaccg agctgtatgg caaagtggga
     2881 gaaattcgtc actggggcct gatccgatac atttctggga tcttaaggaa gaaagtggaa
     2941 gcacttgatg aggcctgcac agaccttctc tcccaccaga aacatttgac agtaggactt
     3001 cctccagaac ctcgagaaaa gactatctct gcacctctgc cctatgaggc gctcactcag
     3061 ctgatagatg aagccagtga aggggatatg agcatttcaa tccttacaca ggaaataatg
     3121 gtatatctag ccatgtatat gcgaacccag cctggcctct ttgctgaaat gtttcgactt
     3181 cgaattggtc tgatcataca agttatggca acagaactgg cccactccct tcgatgctca
     3241 gctgaggaag ccacagaggg cctgatgaat ctcagtcctt cggccatgaa gaatctcctg
     3301 catcacattc tcagcggcaa ggagtttgga gtggaacgaa gcgttcgtcc cactgattca
     3361 aatgtcagtc ctgctatttc tatccacgag attggtgctg ttggagcaac caaaacagaa
     3421 cgaactggga tcatgcagtt aaaaagtgag ataaagcagg tggaatttcg tagactgtca
     3481 atctcagctg agagtcagtc acctggaacc tctatgactc caagtagtgg gtcctttcct
     3541 agtgcatatg atcagcagtc atctaaagat agtcgtcaag gtcaatggca acgccgaaga
     3601 aggctggatg gggcactgaa tagagttcca gttggatttt atcagaaagt atggaaagtt
     3661 ttgcagaagt gtcacggact ttctgttgaa gggtttgtcc ttccttcctc taccactaga
     3721 gagatgactc caggtgagat taaattctct gttcatgtgg agtctgtcct gaatcgtgta
     3781 cctcagccag agtaccgtca gctgctggtt gaagccatcc ttgtcctcac catgctggca
     3841 gatattgaaa ttcatagcat cggaagcatc attgctgtgg aaaaaatagt gcatattgcc
     3901 aatgacttgt tccttcaaga acagaaaacc cttggcgcag atgataccat gttggcaaag
     3961 gatcccgcat ctggcatctg tactcttctg tatgacagtg cacccagtgg caggtttggc
     4021 accatgacct acctctccaa ggcagccgcc acctacgtgc aggagttcct gccccacagc
     4081 atctgtgcca tgcaatgagg gctttggttc ctggcttctg ggagcctttt gacagctggt
     4141 ccctgcctcg gttgattgtg catggaacta aaatgttatt gcctaatcac tccaaccctg
     4201 cccctttctg tcccatcctt cccaagaaga gagaactttt tcgataaact aactactgta
     4261 gaagaagtga acacttacct ggaggctcac cttgcagaac cagtgacaat cttatgagta
     4321 taatgaacac tcagccaggc ctgtcatgat tggctttatt tctttcatca ttcataaaag
     4381 tttgcatgtg tttttattct ctagatctgt taccaatata gttttctaac tcctgtttgg
     4441 ggagcaagtg ttaataataa cttattccta aagcttggtt tttctttttt gtgattttat
     4501 tgtgtatatg agtggtgggt actttgggac attatttcaa atgagtaaac tctaaattgt
     4561 taaatattta tgctgctata aattagaatt acctattcta cgtgcctttt cttaggaaaa
     4621 gagagtactg gatcatggtt ttcttcctta cttacaggtt gcttcacact gagtgataat
     4681 ttaaaattgt ttctatgtag aatcattgtc tctactgttg ccctctctgt gtctgcacac
     4741 ataatgcctc tctcactttg gtgaaatatc aaaaaaatgc aagtacagca tgcatattta
     4801 agaatttctg aactaacact tgaaatcaca ttttataaag attgttggta ctcagagttg
     4861 tatttgcagc agaaaatgca gaataaccca gaaatggagc ccggagccca attccagaga
     4921 ggaagggaaa gaagagacga tgcctggaca atgtcttata catagggaac agagagggaa
     4981 tcagaggtgg cttagttccc tgaagacagc cttatgcgct taatccctgc ctttgaaaaa
     5041 tgggccaata cacaacacag acaataattt gaagcgtctg tattcagcaa agataaatac
     5101 agcagtccag tctataggtg ctctacttag cattcttttc atgtgctcct ggtctctgtc
     5161 tcactcacct actttctact acatccaact caactccctg acaccctatc cccccatcta
     5221 ttcttaaact ctttcatata tattttcact tcctaattct agtgtacata tccctgtata
     5281 tcatggtatc tgtgggcata tgtctgtttc ttataaagac acatatttgt gtgtgtatgt
     5341 gtgtgtgtgt gtgtgtgtgt gtataagcat acatgggaat ttttctgaat gtaagcagtt
     5401 ttgatgtgtc tgtatatcaa tctgtatcta catttgatgg actgagtaag aatgttgcat
     5461 gtgagagaag ggatgtgact gtggactcag attgacacac acagattggt ggaagaatag
     5521 cttcagtcct cttccttcat cccatatgta ggtagccttg cttgacaaat gtttcttcta
     5581 actctccttc ttctgaattg tgcagagact ctgttaactt ccctttttgc acttagataa
     5641 cttcctactt caaccccctc ctttatcttg gatttctaaa gtttttcccc aatattggga
     5701 atggtgaggg tggggatgag agactctctg aacaattctt gcagaaccag tgacaatctt
     5761 atgaatataa tgaataagta agcatatagt agggtgatag aatacagata gtagaaacaa
     5821 agagaggaca gcaaaccttt tcagcacttc tctcagagca aatggaaaca cacagtaagt
     5881 agctttctga ctacattatt ttctggtcac ttttaaagaa agtttacaac ctgttctgaa
     5941 actatttgtc ttttcactgg ttgtaagtgt accccaatct cagggagtat atctgtagtg
     6001 ccacaggcaa aagatccact ccttcccaca ctcatttgcc tgaacttact cgaagggctg
     6061 catttctctg agtttacgaa attgtgtcat tatggtcccc atacagtggt atttaacttt
     6121 taaagcaact tttaagaaaa ctcgacttgt tttttgttca ttttaagtgt gtggtactaa
     6181 aaagacatgt tagacttttt ttaaaaaagc acttatgttt tgaaaataga ataaataata
     6241 agaatttcca attaaatcat gtctggtgcc aatgtgcaaa acttca
//
