LOCUS       NM_001172436            6070 bp    mRNA    linear   PRI 13-OCT-2024
DEFINITION  Homo sapiens phosphorylase kinase regulatory subunit alpha 1
            (PHKA1), transcript variant 3, mRNA.
ACCESSION   NM_001172436
VERSION     NM_001172436.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Bisciglia,M., Froissart,R., Bedat-Millet,A.L., Romero,N.B.,
            Pettazzoni,M., Hogrel,J.Y., Petit,F.M. and Stojkovic,T.
  TITLE     A novel PHKA1 mutation associating myopathy and cognitive
            impairment: Expanding the spectrum of phosphorylase kinase b (PhK)
            deficiency
  JOURNAL   J Neurol Sci 424, 117391 (2021)
   PUBMED   33799212
  REMARK    GeneRIF: A novel PHKA1 mutation associating myopathy and cognitive
            impairment: Expanding the spectrum of phosphorylase kinase b (PhK)
            deficiency.
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6070)
  AUTHORS   Preisler,N., Orngreen,M.C., Echaniz-Laguna,A., Laforet,P.,
            Lonsdorfer-Wolf,E., Doutreleau,S., Geny,B., Akman,H.O., Dimauro,S.
            and Vissing,J.
  TITLE     Muscle phosphorylase kinase deficiency: a neutral metabolic variant
            or a disease?
  JOURNAL   Neurology 78 (4), 265-268 (2012)
   PUBMED   22238410
  REMARK    GeneRIF: muscle PHKA deficiency may present as an almost
            asymptomatic condition, despite a mild impairment of muscle
REFERENCE   4  (bases 1 to 6070)
  AUTHORS   Echaniz-Laguna,A., Akman,H.O., Mohr,M., Tranchant,C.,
            Talmant-Verbist,V., Rolland,M.O. and Dimauro,S.
  TITLE     Muscle phosphorylase b kinase deficiency revisited
  JOURNAL   Neuromuscul Disord 20 (2), 125-127 (2010)
   PUBMED   20080404
REFERENCE   5  (bases 1 to 6070)
  AUTHORS   Orngreen,M.C., Schelhaas,H.J., Jeppesen,T.D., Akman,H.O.,
            Wevers,R.A., Andersen,S.T., ter Laak,H.J., van Diggelen,O.P.,
            DiMauro,S. and Vissing,J.
  TITLE     Is muscle glycogenolysis impaired in X-linked phosphorylase b
            kinase deficiency?
  JOURNAL   Neurology 70 (20), 1876-1882 (2008)
   PUBMED   18401027
  REMARK    GeneRIF: X-linked PHK deficiency causes a mild metabolic myopathy
            with blunted muscle glycogen breakdown and impaired lactate
            production during dynamic exercise, which impairs oxidative
            capacity only marginally
REFERENCE   6  (bases 1 to 6070)
  AUTHORS   Gossen,M., Wullrich,A. and Kilimann,M.W.
  TITLE     Dinucleotide repeat polymorphism within the PHKA1 gene at Xq12-q13
  JOURNAL   Hum Genet 95 (4), 469-470 (1995)
   PUBMED   7705849
REFERENCE   7  (bases 1 to 6070)
  AUTHORS   Wehner,M., Clemens,P.R., Engel,A.G. and Kilimann,M.W.
  TITLE     Human muscle glycogenosis due to phosphorylase kinase deficiency
            associated with a nonsense mutation in the muscle isoform of the
            alpha subunit
  JOURNAL   Hum Mol Genet 3 (11), 1983-1987 (1994)
   PUBMED   7874115
REFERENCE   8  (bases 1 to 6070)
  AUTHORS   Herbert,M., Goldstein,J.L., Rehder,C., Austin,S., Kishnani,P.S. and
            Bali,D.S.
  TITLE     Phosphorylase Kinase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21634085
REFERENCE   9  (bases 1 to 6070)
  AUTHORS   Meyer,H.E., Meyer,G.F., Dirks,H. and Heilmeyer,L.M. Jr.
  TITLE     Localization of phosphoserine residues in the alpha subunit of
            rabbit skeletal muscle phosphorylase kinase
  JOURNAL   Eur J Biochem 188 (2), 367-376 (1990)
   PUBMED   2108025
REFERENCE   10 (bases 1 to 6070)
  AUTHORS   Francke,U., Darras,B.T., Zander,N.F. and Kilimann,M.W.
  TITLE     Assignment of human genes for phosphorylase kinase subunits alpha
            (PHKA) to Xq12-q13 and beta (PHKB) to 16q12-q13
  JOURNAL   Am J Hum Genet 45 (2), 276-282 (1989)
   PUBMED   2757032
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC240504.3, DR002787.1,
            BC143501.1, DA171597.1, DA998603.1, BX647542.1 and AW450116.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001172436.1.
            
            Summary: Phosphorylase kinase is a polymer of 16 subunits, four
            each of alpha, beta, gamma and delta. The alpha subunit includes
            the skeletal muscle and hepatic isoforms, and the skeletal muscle
            isoform is encoded by this gene. The beta subunit is the same in
            both the muscle and hepatic isoforms, and encoded by one gene. The
            gamma subunit also includes the skeletal muscle and hepatic
            isoforms, which are encoded by two different genes. The delta
            subunit is a calmodulin and can be encoded by three different
            genes. The gamma subunits contain the active site of the enzyme,
            whereas the alpha and beta subunits have regulatory functions
            controlled by phosphorylation. The delta subunit mediates the
            dependence of the enzyme on calcium concentration. Mutations in
            this gene cause glycogen storage disease type 9D, also known as
            X-linked muscle glycogenosis. Alternatively spliced transcript
            variants encoding different isoforms have been identified in this
            gene. A pseudogene has been found on chromosome 1.[provided by
            RefSeq, Feb 2010].
            
            Transcript Variant: This variant (3) lacks two alternate in-frame
            exons in the middle and 3' coding regions, respectively, compared
            to variant 1, resulting in a shorter protein (isoform 3), compared
            to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.2329708.1,
                                           SRR14038191.1326693.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-125               AC240504.3         157301-157425
            126-387             DR002787.1         22-283
            388-3937            BC143501.1         9-3558
            3938-4022           DA171597.1         497-581
            4023-4492           DA998603.1         74-543
            4493-6059           BX647542.1         181-1747
            6060-6070           AW450116.1         17-27               c
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.1"
     gene            1..6070
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="phosphorylase kinase regulatory subunit alpha 1"
                     /db_xref="GeneID:5255"
                     /db_xref="HGNC:HGNC:8925"
                     /db_xref="MIM:311870"
     exon            1..504
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     CDS             427..3882
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /EC_number="2.7.11.19"
                     /note="isoform 3 is encoded by transcript variant 3;
                     phosphorylase kinase, alpha 1 (muscle), muscle
                     glycogenosis; phosphorylase kinase alpha M subunit"
                     /codon_start=1
                     /product="phosphorylase b kinase regulatory subunit alpha,
                     skeletal muscle isoform isoform 3"
                     /protein_id="NP_001165907.1"
                     /db_xref="CCDS:CCDS55453.1"
                     /db_xref="GeneID:5255"
                     /db_xref="HGNC:HGNC:8925"
                     /db_xref="MIM:311870"
                     /translation="MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWV
                     RDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESF
                     KYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLD
                     EVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLF
                     GVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLV
                     ELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTY
                     FILDGVFSGNAEQVQEYKEALEAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDR
                     VPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEE
                     IKTILKDKGIYVETIAEVYPIRVQPARILSHIYSSLGCNNRMKLSGRPYRHMGVLGTS
                     KLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQPTITFPI
                     SHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQTGKLSEFLTTSCCTHLSFMDPGPE
                     GKLYSEDYDDNYDYLESGNWMNDYDSTSHDVHMYLPTKLFQASRPSFNLLDSPHPRQE
                     NQVPSVRVEIHLPRDQSGEVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWNTELY
                     NERSATVRELLTELYGKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSHQKHLTVG
                     LPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMVYLAMYMRTQPGLFAEM
                     FRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSV
                     RPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPSSGSFPSAYDQQ
                     SSKDSRQGQWQRRRRLDGALNRVPVGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTP
                     GEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIAND
                     LFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHS
                     ICAMQ"
     exon            505..663
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            664..711
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            712..880
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            881..963
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            964..1044
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1143
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1290
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1344
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1467
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1563
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1671
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1750
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1885
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1995
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2140
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2219
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2386
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2478
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..2618
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2619..2775
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2776..2855
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2856..2934
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2935..3064
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3166
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3282
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3283..3453
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3507
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3508..3708
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     exon            3709..6070
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4237..4242
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: AATAAT"
     polyA_site      4256
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="major polyA site"
     regulatory      4328..4333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: AGTAAA"
     polyA_site      4350
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
     regulatory      6021..6026
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: AATAAG"
     regulatory      6035..6040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
                     /note="hexamer: ATTAAA"
     polyA_site      6040
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
     polyA_site      6070
                     /gene="PHKA1"
                     /gene_synonym="PHKA"
ORIGIN      
        1 gcctcccgct cgcgtctccc gcaaggtgga ggcggcgcag cggtcgttga cctgttggcg
       61 gcggccgggc gggaggctgt gggccgcggc ggtctcccgg ggttgagggg aggatgcagg
      121 agtcacacgc ggaggccgga ctggactaag ggggcaggag cggcgcctgg ggcgctgcgg
      181 cggggctcta gaccgcggga agccagtgct agcgctggcg cgtggtgggc ccctgaggcc
      241 gccggagtcc ggagaggtac gcggaatccg cggcgccagg cctgagcggt gggagggctc
      301 tgcggggcct ggtgttcagg cgtcccacca cgagggtgga gcagcgttgg atacttgttc
      361 cttagggacc gaaggctccg gtggcacccg ggctatttct cagaggacaa ttagtaacgt
      421 gtcgccatga ggagccggag taactccggg gtccggctgg acggctacgc tcgactggtg
      481 caacagacca tcctgtgcca tcagaatcca gtgactggct tgcttccagc cagctatgat
      541 cagaaagatg cttgggtccg agataatgtg tacagcatct tggctgtgtg gggtttgggc
      601 ctggcctatc ggaagaatgc agaccgggat gaggataagg caaaggccta tgaattggag
      661 cagagtgtag tgaagctgat gagaggacta ctgcactgca tgatcagaca ggtggataaa
      721 gtagaatcct tcaaatatag tcagagtact aaggatagcc tccatgcaaa gtacaacacc
      781 aaaacctgtg ccactgtagt gggtgatgat caatggggac acctgcagtt ggatgctacc
      841 tctgtgtacc tgctcttctt agcccaaatg actgcctcag gactccatat catccacagc
      901 ctagatgaag tcaatttcat acagaacctt gtgttttaca ttgaagctgc atataaaact
      961 gctgacttcg ggatatggga acgtggagac aagaccaacc aagggatctc agagttgaat
     1021 gccagttcag ttggaatggc aaaggcagcc ctggaagcat tagatgaact ggatctgttt
     1081 ggtgtgaaag gtgggcctca atcagttatc catgtcctgg ctgatgaagt acagcactgc
     1141 cagtctatcc taaattcact actgccccgt gcttcaacat caaaagaggt tgatgctagt
     1201 ctactctcag tggtttcctt ccctgccttt gcagtagagg atagccagtt ggtggagctc
     1261 acaaaacagg aaatcatcac caagcttcag ggtcgttatg gttgctgtcg ctttctacga
     1321 gatggatata aaactcctaa agaggatccc aatcgtctgt actatgaacc agctgagctg
     1381 aagctatttg aaaacattga gtgtgaatgg ccattgttct ggacatactt tattcttgat
     1441 ggggtcttca gtggcaatgc agaacaggtt caagaatata aagaggctct tgaagcagtc
     1501 ctcatcaagg gcaaaaatgg agtcccactt ctgccagagc tgtacagtgt tcctcctgac
     1561 agggtcgatg aagaatatca gaatcctcac actgtggacc gagtccccat ggggaaattg
     1621 cctcacatgt ggggtcagtc tctatacatt ttaggaagct tgatggcaga gggattttta
     1681 gcccctggag aaattgatcc cctgaatcgc aggttttcta ctgtaccgaa gcccgatgtt
     1741 gtggttcaag tctccattct agctgaaaca gaagaaatca agaccatttt gaaggacaag
     1801 ggaatttacg tggagaccat tgctgaggta taccccatca gagtacaacc agctcgtatt
     1861 ctcagccaca tttattccag cctaggatgc aacaatagaa tgaaactcag tggacgaccc
     1921 tacagacaca tgggagtgct tggaacttca aaactctatg acattcggaa aactatcttt
     1981 actttcactc cacagtttat agaccagcaa cagttctacc tggctctgga caacaagatg
     2041 atagtggaaa tgcttagaac agacctctcc tacctctgta gccgctggcg gatgacaggc
     2101 cagcccacca tcaccttccc catctcacac agcatgcttg atgaagatgg aacaagcttg
     2161 aattcaagta tcctggcagc actccgaaaa atgcaagatg ggtattttgg tggggcaagg
     2221 gttcaaacag gtaaattgtc agagtttttg acaacatctt gttgcacaca cttgagcttc
     2281 atggaccctg gacctgaggg taagctgtac agtgaagatt atgatgacaa ctatgattac
     2341 ctggaatctg gcaactggat gaatgattat gattcaacca gtcatgatgt tcacatgtat
     2401 cttcctacga agttatttca ggcttcccgg ccttcattca acttacttga ttcacctcat
     2461 ccccgacagg agaaccaggt tccctctgtt cgtgtagaaa tacatcttcc tagagaccag
     2521 tctggggagg tggactttaa agcactggtt ttacagttga aggagacctc aagcttacag
     2581 gaacaagctg atatcctcta tatgctgtat actatgaaag gacctgactg gaacactgaa
     2641 ttgtataatg aacggagtgc tacagtgaga gagcttctta ccgagctgta tggcaaagtg
     2701 ggagaaattc gtcactgggg cctgatccga tacatttctg ggatcttaag gaagaaagtg
     2761 gaagcacttg atgaggcctg cacagacctt ctctcccacc agaaacattt gacagtagga
     2821 cttcctccag aacctcgaga aaagactatc tctgcacctc tgccctatga ggcgctcact
     2881 cagctgatag atgaagccag tgaaggggat atgagcattt caatccttac acaggaaata
     2941 atggtatatc tagccatgta tatgcgaacc cagcctggcc tctttgctga aatgtttcga
     3001 cttcgaattg gtctgatcat acaagttatg gcaacagaac tggcccactc ccttcgatgc
     3061 tcagctgagg aagccacaga gggcctgatg aatctcagtc cttcggccat gaagaatctc
     3121 ctgcatcaca ttctcagcgg caaggagttt ggagtggaac gaagcgttcg tcccactgat
     3181 tcaaatgtca gtcctgctat ttctatccac gagattggtg ctgttggagc aaccaaaaca
     3241 gaacgaactg ggatcatgca gttaaaaagt gagataaagc agtcacctgg aacctctatg
     3301 actccaagta gtgggtcctt tcctagtgca tatgatcagc agtcatctaa agatagtcgt
     3361 caaggtcaat ggcaacgccg aagaaggctg gatggggcac tgaatagagt tccagttgga
     3421 ttttatcaga aagtatggaa agttttgcag aagtgtcacg gactttctgt tgaagggttt
     3481 gtccttcctt cctctaccac tagagagatg actccaggtg agattaaatt ctctgttcat
     3541 gtggagtctg tcctgaatcg tgtacctcag ccagagtacc gtcagctgct ggttgaagcc
     3601 atccttgtcc tcaccatgct ggcagatatt gaaattcata gcatcggaag catcattgct
     3661 gtggaaaaaa tagtgcatat tgccaatgac ttgttccttc aagaacagaa aacccttggc
     3721 gcagatgata ccatgttggc aaaggatccc gcatctggca tctgtactct tctgtatgac
     3781 agtgcaccca gtggcaggtt tggcaccatg acctacctct ccaaggcagc cgccacctac
     3841 gtgcaggagt tcctgcccca cagcatctgt gccatgcaat gagggctttg gttcctggct
     3901 tctgggagcc ttttgacagc tggtccctgc ctcggttgat tgtgcatgga actaaaatgt
     3961 tattgcctaa tcactccaac cctgcccctt tctgtcccat ccttcccaag aagagagaac
     4021 tttttcgata aactaactac tgtagaagaa gtgaacactt acctggaggc tcaccttgca
     4081 gaaccagtga caatcttatg agtataatga acactcagcc aggcctgtca tgattggctt
     4141 tatttctttc atcattcata aaagtttgca tgtgttttta ttctctagat ctgttaccaa
     4201 tatagttttc taactcctgt ttggggagca agtgttaata ataacttatt cctaaagctt
     4261 ggtttttctt ttttgtgatt ttattgtgta tatgagtggt gggtactttg ggacattatt
     4321 tcaaatgagt aaactctaaa ttgttaaata tttatgctgc tataaattag aattacctat
     4381 tctacgtgcc ttttcttagg aaaagagagt actggatcat ggttttcttc cttacttaca
     4441 ggttgcttca cactgagtga taatttaaaa ttgtttctat gtagaatcat tgtctctact
     4501 gttgccctct ctgtgtctgc acacataatg cctctctcac tttggtgaaa tatcaaaaaa
     4561 atgcaagtac agcatgcata tttaagaatt tctgaactaa cacttgaaat cacattttat
     4621 aaagattgtt ggtactcaga gttgtatttg cagcagaaaa tgcagaataa cccagaaatg
     4681 gagcccggag cccaattcca gagaggaagg gaaagaagag acgatgcctg gacaatgtct
     4741 tatacatagg gaacagagag ggaatcagag gtggcttagt tccctgaaga cagccttatg
     4801 cgcttaatcc ctgcctttga aaaatgggcc aatacacaac acagacaata atttgaagcg
     4861 tctgtattca gcaaagataa atacagcagt ccagtctata ggtgctctac ttagcattct
     4921 tttcatgtgc tcctggtctc tgtctcactc acctactttc tactacatcc aactcaactc
     4981 cctgacaccc tatcccccca tctattctta aactctttca tatatatttt cacttcctaa
     5041 ttctagtgta catatccctg tatatcatgg tatctgtggg catatgtctg tttcttataa
     5101 agacacatat ttgtgtgtgt atgtgtgtgt gtgtgtgtgt gtgtgtataa gcatacatgg
     5161 gaatttttct gaatgtaagc agttttgatg tgtctgtata tcaatctgta tctacatttg
     5221 atggactgag taagaatgtt gcatgtgaga gaagggatgt gactgtggac tcagattgac
     5281 acacacagat tggtggaaga atagcttcag tcctcttcct tcatcccata tgtaggtagc
     5341 cttgcttgac aaatgtttct tctaactctc cttcttctga attgtgcaga gactctgtta
     5401 acttcccttt ttgcacttag ataacttcct acttcaaccc cctcctttat cttggatttc
     5461 taaagttttt ccccaatatt gggaatggtg agggtgggga tgagagactc tctgaacaat
     5521 tcttgcagaa ccagtgacaa tcttatgaat ataatgaata agtaagcata tagtagggtg
     5581 atagaataca gatagtagaa acaaagagag gacagcaaac cttttcagca cttctctcag
     5641 agcaaatgga aacacacagt aagtagcttt ctgactacat tattttctgg tcacttttaa
     5701 agaaagttta caacctgttc tgaaactatt tgtcttttca ctggttgtaa gtgtacccca
     5761 atctcaggga gtatatctgt agtgccacag gcaaaagatc cactccttcc cacactcatt
     5821 tgcctgaact tactcgaagg gctgcatttc tctgagttta cgaaattgtg tcattatggt
     5881 ccccatacag tggtatttaa cttttaaagc aacttttaag aaaactcgac ttgttttttg
     5941 ttcattttaa gtgtgtggta ctaaaaagac atgttagact ttttttaaaa aagcacttat
     6001 gttttgaaaa tagaataaat aataagaatt tccaattaaa tcatgtctgg tgccaatgtg
     6061 caaaacttca
//
