LOCUS       NM_017934              11926 bp    mRNA    linear   PRI 23-MAR-2025
DEFINITION  Homo sapiens PHIP subunit of CUL4-Ring ligase complex (PHIP), mRNA.
ACCESSION   NM_017934
VERSION     NM_017934.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Tirado-Class,N., Hathaway,C., Chung,W.K. and Dungrawala,H.
  TITLE     PHIP variants associated with Chung-Jansen syndrome disrupt
            replication fork stability and genome integrity
  JOURNAL   Cold Spring Harb Mol Case Stud 8 (5) (2022) In press
   PUBMED   35863899
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11926)
  AUTHORS   Morgan,M.A.J., Popova,I.K., Vaidya,A., Burg,J.M., Marunde,M.R.,
            Rendleman,E.J., Dumar,Z.J., Watson,R., Meiners,M.J., Howard,S.A.,
            Khalatyan,N., Vaughan,R.M., Rothbart,S.B., Keogh,M.C. and
            Shilatifard,A.
  TITLE     A trivalent nucleosome interaction by PHIP/BRWD2 is disrupted in
            neurodevelopmental disorders and cancer
  JOURNAL   Genes Dev 35 (23-24), 1642-1656 (2021)
   PUBMED   34819353
  REMARK    GeneRIF: A trivalent nucleosome interaction by PHIP/BRWD2 is
            disrupted in neurodevelopmental disorders and cancer.
REFERENCE   3  (bases 1 to 11926)
  AUTHORS   Kim,D.K., Redon,C.E., Aladjem,M.I., Kim,H.K. and Jang,S.M.
  TITLE     Molecular double clips within RepID WD40 domain control chromatin
            binding and CRL4-substrate assembly
  JOURNAL   Biochem Biophys Res Commun 567, 208-214 (2021)
   PUBMED   34171797
  REMARK    GeneRIF: Molecular double clips within RepID WD40 domain control
            chromatin binding and CRL4-substrate assembly.
REFERENCE   4  (bases 1 to 11926)
  AUTHORS   Townsend,A., Lora,G., Engel,J., Tirado-Class,N. and Dungrawala,H.
  TITLE     DCAF14 promotes stalled fork stability to maintain genome integrity
  JOURNAL   Cell Rep 34 (4), 108669 (2021)
   PUBMED   33503431
  REMARK    GeneRIF: DCAF14 promotes stalled fork stability to maintain genome
            integrity.
REFERENCE   5  (bases 1 to 11926)
  AUTHORS   Raich,L., Meier,K., Gunther,J., Christ,C.D., Noe,F. and Olsson,S.
  TITLE     Discovery of a hidden transient state in all bromodomain families
  JOURNAL   Proc Natl Acad Sci U S A 118 (4) (2021)
   PUBMED   33468647
  REMARK    GeneRIF: Discovery of a hidden transient state in all bromodomain
            families.
REFERENCE   6  (bases 1 to 11926)
  AUTHORS   Filippakopoulos,P., Picaud,S., Mangos,M., Keates,T., Lambert,J.P.,
            Barsyte-Lovejoy,D., Felletar,I., Volkmer,R., Muller,S., Pawson,T.,
            Gingras,A.C., Arrowsmith,C.H. and Knapp,S.
  TITLE     Histone recognition and large-scale structural analysis of the
            human bromodomain family
  JOURNAL   Cell 149 (1), 214-231 (2012)
   PUBMED   22464331
REFERENCE   7  (bases 1 to 11926)
  AUTHORS   Bai,S.W., Herrera-Abreu,M.T., Rohn,J.L., Racine,V., Tajadura,V.,
            Suryavanshi,N., Bechtel,S., Wiemann,S., Baum,B. and Ridley,A.J.
  TITLE     Identification and characterization of a set of conserved and new
            regulators of cytoskeletal organization, cell morphology and
            migration
  JOURNAL   BMC Biol 9, 54 (2011)
   PUBMED   21834987
  REMARK    Erratum:[BMC Biol. 2024 Oct 10;22(1):228. doi:
            10.1186/s12915-024-02036-3. PMID: 39390519]
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 11926)
  AUTHORS   Podcheko,A., Northcott,P., Bikopoulos,G., Lee,A., Bommareddi,S.R.,
            Kushner,J.A., Farhang-Fallah,J. and Rozakis-Adcock,M.
  TITLE     Identification of a WD40 repeat-containing isoform of PHIP as a
            novel regulator of beta-cell growth and survival
  JOURNAL   Mol Cell Biol 27 (18), 6484-6496 (2007)
   PUBMED   17636024
REFERENCE   9  (bases 1 to 11926)
  AUTHORS   Obuse,C., Yang,H., Nozaki,N., Goto,S., Okazaki,T. and Yoda,K.
  TITLE     Proteomics analysis of the centromere complex from HeLa interphase
            cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of
            the CEN-complex, while BMI-1 is transiently co-localized with the
            centromeric region in interphase
  JOURNAL   Genes Cells 9 (2), 105-120 (2004)
   PUBMED   15009096
REFERENCE   10 (bases 1 to 11926)
  AUTHORS   Farhang-Fallah,J., Yin,X., Trentin,G., Cheng,A.M. and
            Rozakis-Adcock,M.
  TITLE     Cloning and characterization of PHIP, a novel insulin receptor
            substrate-1 pleckstrin homology domain interacting protein
  JOURNAL   J Biol Chem 275 (51), 40492-40497 (2000)
   PUBMED   11018022
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA500781.1, DQ924532.1,
            BF934406.1, AL450327.12 and BQ006356.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_017934.6.
            
            Summary: This gene encodes a protein that binds to the insulin
            receptor substrate 1 protein and regulates glucose transporter
            translocation in skeletal muscle cells. The encoded protein may
            also regulate growth and survival of pancreatic beta cells.
            Elevated copy number of this gene may be associated with melanoma
            severity and the encoded protein may promote melanoma metastasis in
            human patients. [provided by RefSeq, Oct 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.83153.1,
                                           SRR1803615.80009.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000275034.5/ ENSP00000275034.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               DA500781.1         41-226
            187-2173            DQ924532.1         1-1987
            2174-2174           BF934406.1         268-268
            2175-5718           DQ924532.1         1989-5532
            5719-11297          AL450327.12        166972-172550       c
            11298-11926         BQ006356.1         18-646              c
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q14.1"
     gene            1..11926
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /note="PHIP subunit of CUL4-Ring ligase complex"
                     /db_xref="GeneID:55023"
                     /db_xref="HGNC:HGNC:15673"
                     /db_xref="MIM:612870"
     exon            1..226
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..5652
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /note="DDB1 and CUL4 associated factor 14; pleckstrin
                     homology domain interacting protein; IRS-1 PH
                     domain-binding protein; WD repeat-containing protein 11"
                     /codon_start=1
                     /product="PH-interacting protein"
                     /protein_id="NP_060404.4"
                     /db_xref="CCDS:CCDS4987.1"
                     /db_xref="GeneID:55023"
                     /db_xref="HGNC:HGNC:15673"
                     /db_xref="MIM:612870"
                     /translation="MSCERKGLSELRSELYFLIARFLEDGPCQQAAQVLIREVAEKEL
                     LPRRTDWTGKEHPRTYQNLVKYYRHLAPDHLLQICHRLGPLLEQEIPQSVPGVQTLLG
                     AGRQSLLRTNKSCKHVVWKGSALAALHCGRPPESPVNYGSPPSIADTLFSRKLNGKYR
                     LERLVPTAVYQHMKMHKRILGHLSSVYCVTFDRTGRRIFTGSDDCLVKIWATDDGRLL
                     ATLRGHAAEISDMAVNYENTMIAAGSCDKMIRVWCLRTCAPLAVLQGHSASITSLQFS
                     PLCSGSKRYLSSTGADGTICFWLWDAGTLKINPRPAKFTERPRPGVQMICSSFSAGGM
                     FLATGSTDHIIRVYFFGSGQPEKISELEFHTDKVDSIQFSNTSNRFVSGSRDGTARIW
                     QFKRREWKSILLDMATRPAGQNLQGIEDKITKMKVTMVAWDRHDNTVITAVNNMTLKV
                     WNSYTGQLIHVLMGHEDEVFVLEPHPFDPRVLFSAGHDGNVIVWDLARGVKIRSYFNM
                     IEGQGHGAVFDCKCSPDGQHFACTDSHGHLLIFGFGSSSKYDKIADQMFFHSDYRPLI
                     RDANNFVLDEQTQQAPHLMPPPFLVDVDGNPHPSRYQRLVPGRENCREEQLIPQMGVT
                     SSGLNQVLSQQANQEISPLDSMIQRLQQEQDLRRSGEAVISNTSRLSRGSISSTSEVH
                     SPPNVGLRRSGQIEGVRQMHSNAPRSEIATERDLVAWSRRVVVPELSAGVASRQEEWR
                     TAKGEEEIKTYRSEEKRKHLTVPKENKIPTVSKNHAHEHFLDLGESKKQQTNQHNYRT
                     RSALEETPRPSEEIENGSSSSDEGEVVAVSGGTSEEEERAWHSDGSSSDYSSDYSDWT
                     ADAGINLQPPKKVPKNKTKKAESSSDEEEESEKQKQKQIKKEKKKVNEEKDGPISPKK
                     KKPKERKQKRLAVGELTENGLTLEEWLPSTWITDTIPRRCPFVPQMGDEVYYFRQGHE
                     AYVEMARKNKIYSINPKKQPWHKMELREQELMKIVGIKYEVGLPTLCCLKLAFLDPDT
                     GKLTGGSFTMKYHDMPDVIDFLVLRQQFDDAKYRRWNIGDRFRSVIDDAWWFGTIESQ
                     EPLQLEYPDSLFQCYNVCWDNGDTEKMSPWDMELIPNNAVFPEELGTSVPLTDGECRS
                     LIYKPLDGEWGTNPRDEECERIVAGINQLMTLDIASAFVAPVDLQAYPMYCTVVAYPT
                     DLSTIKQRLENRFYRRVSSLMWEVRYIEHNTRTFNEPGSPIVKSAKFVTDLLLHFIKD
                     QTCYNIIPLYNSMKKKVLSDSEDEEKDADVPGTSTRKRKDHQPRRRLRNRAQSYDIQA
                     WKKQCEELLNLIFQCEDSEPFRQPVDLLEYPDYRDIIDTPMDFATVRETLEAGNYESP
                     MELCKDVRLIFSNSKAYTPSKRSRIYSMSLRLSAFFEEHISSVLSDYKSALRFHKRNT
                     ITKRRKKRNRSSSVSSSAASSPERKKRILKPQLKSESSTSAFSTPTRSIPPRHNAAQI
                     NGKTESSSVVRTRSNRVVVDPVVTEQPSTSSAAKTFITKANASAIPGKTILENSVKHS
                     KALNTLSSPGQSSFSHGTRNNSAKENMEKEKPVKRKMKSSVLPKASTLSKSSAVIEQG
                     DCKNNALVPGTIQVNGHGGQPSKLVKRGPGRKPKVEVNTNSGEIIHKKRGRKPKKLQY
                     AKPEDLEQNNVHPIRDEVLPSSTCNFLSETNNVKEDLLQKKNRGGRKPKRKMKTQKLD
                     ADLLVPASVKVLRRSNRKKIDDPIDEEEEFEELKGSEPHMRTRNQGRRTAFYNEDDSE
                     EEQRQLLFEDTSLTFGTSSRGRVRKLTEKAKANLIGW"
     exon            227..285
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            286..315
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            316..375
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            376..526
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            527..625
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            626..786
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            787..1008
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1109
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1180
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1281
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1322
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1421
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1575
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1710
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1711..1839
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..2065
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2203
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            2204..2387
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2505
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2646
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2723
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            2724..2955
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            2956..3075
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3183
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3308
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3309..3391
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3392..3503
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3504..3565
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3566..3721
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3722..3842
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3843..3968
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            3969..4038
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            4039..4089
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            4090..4239
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4392
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            4393..4556
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            4557..4816
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            4817..5014
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     exon            5015..11926
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10453..10458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /note="hexamer: AATAGA"
     polyA_site      10474
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /note="major polyA site"
     regulatory      10749..10754
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /note="hexamer: AATAAA"
     polyA_site      10780
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
     regulatory      11900..11905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
                     /note="hexamer: AATAAA"
     polyA_site      11926
                     /gene="PHIP"
                     /gene_synonym="BRWD2; CHUJANS; DCAF14; DIDOD; ndrp; RepID;
                     WDR11"
ORIGIN      
        1 agtcgctgcc tgagagttgt tgtttcctcc tcctcctcct ccgcctccgc cgccgttgct
       61 tgaatggtgg agccgaagct cggctcgtga acacacactg acagctatag ggcaggcggc
      121 ggcaccgtcc ccgcttcccc tcggcggcgg ggtgtcccgt cggcggccct gaagtgaccc
      181 ataaacatgt cttgtgagag gaaaggcctc tcggagctgc gatcggagct ctacttcctc
      241 atcgcccggt tcctggaaga tggaccctgt cagcaggcgg ctcaggtgct gatccgcgag
      301 gtggccgaga aggagctgct gccccggcgc accgactgga ccgggaagga gcatcccagg
      361 acctaccaga atctggtgaa gtattacaga cacttagcac ctgatcactt gctgcaaata
      421 tgtcatcgac taggacctct tcttgaacaa gaaattcctc aaagtgttcc tggagtacaa
      481 actttattag gagctggaag acagtcttta ctacgcacaa ataaaagctg caagcatgtt
      541 gtgtggaaag gatctgctct ggctgcgttg cactgtggaa gaccacctga gtcaccagtt
      601 aactatggta gcccacccag cattgcggat actctgtttt caaggaagct gaatgggaaa
      661 tacagacttg agcgacttgt tccaactgca gtgtatcagc acatgaaaat gcataaacga
      721 attcttggac acttgtcatc tgtgtactgt gtaacttttg atcgaactgg cagacggata
      781 tttactggtt ctgatgactg tcttgtgaaa atatgggcaa cagatgatgg gaggttgtta
      841 gctaccttaa gaggacatgc tgctgaaata tcagacatgg ctgtaaacta tgagaatacc
      901 atgatagcag ctggaagttg tgataaaatg atccgagtct ggtgtcttcg aacctgtgca
      961 cctttggctg ttcttcaggg ccatagtgca tctattacat cactacagtt ctcaccattg
     1021 tgcagtggct caaagagata tctatcttct actggggcag atggcactat ttgtttttgg
     1081 ctctgggatg ctggaaccct taaaataaac ccaagacctg caaaatttac agagcgccct
     1141 cggcctggag ttcaaatgat ctgttcttct tttagtgctg gtggaatgtt tctggcgacg
     1201 ggaagcacag atcatattat tcgggtttat ttttttggat caggtcagcc agagaaaata
     1261 tcagaattgg agtttcatac tgacaaagtt gacagtatcc agttttccaa cactagtaac
     1321 aggtttgtaa gtggcagtcg tgatgggaca gcacgtattt ggcaatttaa acgaagagag
     1381 tggaagagca ttttgttgga tatggctact cgtccagcag gccaaaacct tcaaggaata
     1441 gaagataaaa tcacaaaaat gaaggttact atggtagctt gggatcgaca tgacaataca
     1501 gttataactg cagttaataa catgactctg aaagtttgga attcttacac tggtcaacta
     1561 attcatgtcc tgatgggtca tgaagatgag gtatttgttc ttgaaccaca cccgttcgat
     1621 cctagagttc tcttttctgc tggtcatgat ggaaacgtga tagtgtggga tctggcaaga
     1681 ggagtcaaaa tacgatctta tttcaatatg attgaaggcc aaggacatgg cgcagtattt
     1741 gactgcaaat gctctcctga tggtcagcat tttgcatgca cagactctca tggacatctt
     1801 ttaatttttg gctttgggtc cagtagcaaa tatgacaaga tagcagatca gatgttcttt
     1861 catagtgatt atcggccact tattcgtgat gccaacaatt ttgtattaga tgaacagact
     1921 cagcaagcac ctcatcttat gcctccccct tttttggttg atgttgatgg taaccctcat
     1981 ccatcaagat atcaaagatt agttcctggc cgtgaaaatt gcagggagga gcaactcatc
     2041 cctcagatgg gagtaacttc ctcaggactg aatcaagttt taagtcagca agcaaaccag
     2101 gagatcagcc cactggacag catgattcaa agactacaac aggagcaaga cctgagacgt
     2161 tctggtgaag cagttatcag taataccagc cgtttaagta gaggctccat aagttctacc
     2221 tcagaggttc attcaccacc aaacgtagga ctaagacgta gtggacaaat tgaaggtgta
     2281 cggcaaatgc acagcaacgc accaagaagt gaaatagcca cagagcggga tctggtagct
     2341 tggagtcgaa gggtggtagt acccgagcta tcagctggtg tagccagtag gcaagaagaa
     2401 tggagaactg caaagggaga agaagaaata aagacttaca ggtcagaaga gaaaagaaaa
     2461 cacttaactg ttccaaaaga gaataaaata cccactgtct caaagaatca tgctcatgag
     2521 catttcctgg atcttggaga atccaaaaag caacagacaa atcaacacaa ttatcgtaca
     2581 agatctgcat tggaagagac tcctagaccc tcagaagaga tagaaaatgg cagtagttct
     2641 tcagatgaag gcgaagtagt tgctgtcagt ggtggaacat ccgaagaaga agagagagca
     2701 tggcacagtg atggcagttc tagtgactac tccagtgatt actctgactg gacagcagat
     2761 gcaggaatta atctgcagcc accaaagaaa gttcctaaga ataaaaccaa gaaagcagaa
     2821 agcagttcag atgaagaaga agaatctgaa aaacagaagc aaaaacagat taaaaaggaa
     2881 aagaaaaaag taaatgaaga aaaagatgga ccaatatcac caaagaaaaa gaagcccaaa
     2941 gaaagaaaac aaaagagatt ggctgtggga gaactaactg aaaatggttt gacattagaa
     3001 gaatggttgc catcaacatg gattacagat accattcccc gaagatgtcc atttgtgcca
     3061 cagatgggtg atgaggttta ttatttccga caaggacatg aagcctatgt cgaaatggcc
     3121 cggaaaaata aaatatatag tatcaatccc aaaaaacaac catggcataa aatggagcta
     3181 cgggaacaag aacttatgaa aatagttggc ataaagtatg aagtgggatt acctaccctt
     3241 tgctgcctta aacttgcttt tctagatcct gatactggta aactgactgg cggatcattt
     3301 accatgaaat accatgatat gcctgacgtt atagatttcc tagtcttgag acaacaattt
     3361 gatgatgcaa aatacaggcg atggaatata ggtgaccgct tcaggtctgt catagatgat
     3421 gcctggtggt ttggaacaat cgaaagccag gaacctcttc aacttgagta ccctgatagt
     3481 ctgtttcaat gctacaatgt ttgctgggac aatggagata cagaaaagat gagtccttgg
     3541 gatatggagc ttatacctaa taatgctgta tttcctgaag aactaggtac cagtgttcct
     3601 ttaactgatg gtgagtgcag atcactaatc tataaacctc ttgatggaga atggggtacc
     3661 aatcccaggg atgaagaatg tgaaagaatt gtggcaggaa taaaccagtt gatgacacta
     3721 gatattgcct cagcatttgt ggcccccgtg gatctgcaag cctatcccat gtattgcaca
     3781 gtagtggcat atccaacgga tctaagtaca attaaacaaa gactggaaaa caggttttac
     3841 aggcgggttt cttccctaat gtgggaagtt cgatatatag agcataatac acgaacattt
     3901 aatgagcctg gaagccctat tgtgaaatct gctaaattcg tgactgatct tcttctacat
     3961 tttataaagg atcagacttg ttataacata attccacttt ataattcaat gaagaagaaa
     4021 gttttgtctg attctgagga tgaagagaaa gatgctgatg tgccaggaac ttctactcga
     4081 aaaaggaagg accatcagcc tagaagaaga ttacgtaata gagcccagtc ttacgatatt
     4141 caagcatgga agaaacagtg tgaagaattg ttaaatctca tatttcaatg tgaagattca
     4201 gagcctttcc gtcagccggt agatctcctt gaatatccag actacagaga catcattgac
     4261 actccaatgg attttgctac cgttagagaa actttagagg ctgggaatta tgagtcacca
     4321 atggagttat gtaaagatgt cagacttatt ttcagtaatt ccaaagcata tacaccaagc
     4381 aaaagatcaa ggatttacag catgagtttg cgcctgtctg ctttctttga agaacacatt
     4441 agttcagttt tatcagatta taaatctgct cttcgttttc ataaaagaaa taccataacc
     4501 aaaaggagga agaaaagaaa cagaagcagc tctgtttcca gtagtgctgc atcaagccct
     4561 gaaaggaaaa aaaggatctt aaaaccccag ctaaaatcag aaagctctac ctctgcattc
     4621 tctacaccta cacgatcaat accgccaaga cacaatgctg ctcagataaa cggtaaaaca
     4681 gaatctagtt ctgtggttcg aaccagaagc aaccgagtgg ttgtagatcc agttgtcact
     4741 gagcaaccat ctacttcttc agctgcaaag acttttatta caaaagctaa tgcatctgca
     4801 ataccaggga aaacaatact agagaattct gtgaaacatt ccaaagcttt gaatactctt
     4861 tccagtcctg gtcaatccag ttttagtcat ggcactagga ataattctgc aaaagaaaac
     4921 atggaaaagg aaaagccagt caaacgtaaa atgaagtcat ctgtactccc aaaggcgtcc
     4981 actctttcaa agtcatcagc tgtcattgag caaggagatt gtaagaacaa cgctcttgta
     5041 ccaggaacca ttcaagtaaa tggccatgga ggacagccat caaaacttgt gaagagggga
     5101 cctggaagga aacctaaagt agaagttaat accaatagtg gtgaaattat acacaagaaa
     5161 aggggtagaa agcccaaaaa gctacagtat gcaaagccag aagatttaga gcaaaataat
     5221 gtgcatccca tcagagatga agtacttcct tcttcaacat gcaattttct ttctgaaact
     5281 aataatgtaa aggaagattt gttacagaaa aagaatcgtg gaggtaggaa gcccaaaagg
     5341 aagatgaaga cacaaaaatt agatgcagat ctcctagtcc ctgcaagtgt caaagtgtta
     5401 aggagaagta accgaaaaaa gatagatgat cctatagatg aggaagaaga gtttgaagaa
     5461 ctcaaaggct ctgaacccca catgagaact agaaatcaag gtcgaaggac agctttctat
     5521 aatgaggatg actctgaaga ggagcaaagg cagctgttgt tcgaagacac ctctttaact
     5581 tttggaactt ctagtagagg acgagtccga aagttgactg aaaaagcaaa agctaattta
     5641 attggttggt aacttgtacc aaaatatttt acttcaaaat ctataaagca ggtacagtta
     5701 aggaataagt agaactaagg cttctgcttc cttgctgctg tggtggagta gggaatgtta
     5761 tgatttgatt tgcaaaaaaa aaaaaaaaaa aaaaaacaaa cttacgagac acttcacttc
     5821 tttaaatgaa tatatatata tatatataaa tatatacata tatattttaa atgtttgcta
     5881 tttattgccc ttagataggt tatgcatttt cacattcatt tcattcactg tttgaaacga
     5941 aagaaattga tctataatga attcctgatt tatttataga aaagacaatt ctgctacact
     6001 ctttaggagc ataatattcc tagtgataga gcatttcatg ttaaaatgaa ttatttttga
     6061 ccaagtgagg tacatttcta ttgatacaag tcatgcccct aaaagataga tgttatacag
     6121 agtagcagtg aattaagaaa tgtttcctct aaaagataaa tataaaattt ccaccatttg
     6181 ataccctaaa cagtttaatt ttaattatag aatgttcatc tacaaagtat ctagaggagg
     6241 cttttgcttt attccatttc tgccaaacaa gagaaaatgt actgatgaag ggaaacatat
     6301 ccacattgga aaacatttga ctgtctaatt tttcagacct tgattcttat atcactcaat
     6361 ctctgtttat tgtgccaaag actgagaatc agtgcagtgg aaagcctgtt tttgacgtca
     6421 ggacagcata cacttttcaa tattggaaag gctatatatt ctaaagagca agttattaaa
     6481 gagttatgct gagatatatc tttttttggt actaatagaa aataatgcac tggtgggtcc
     6541 tttgacagag atgttctaga ggaaatgttt aagtggttaa aggattcaag gaaaatacag
     6601 gaaaaaaggt atgggatttg atgtttactt gttgatctgg attaatgtca tttctaaact
     6661 ttagacatat ctaatagcct aaaatgactt acaaagaaac gtgtctgtgt gtgtatgtgt
     6721 atatattgat aaatgggtat aattaaatat atatacaaac acatacttat atactattac
     6781 ccaggtataa attctttttt caggattttt tagatagcag tagaataaaa ataattttaa
     6841 aatgtcatac tttatagttt aattttaagg gggaaattgg ttattttcaa ttattaaagg
     6901 ttaaaatagg ccaaatcact ttttatgcaa tcatgtttta tacagtggtc tcattgcaca
     6961 ttgtgttttt tctgcacttt ttacggtatc cttatcatgc tggtatgtca atatacaccc
     7021 taaagaaaaa ctccatttaa tgattgtgcc ttgtattcta ttattttttg catcattagt
     7081 aaaattaagt tgtctattgt aaatgataac tatatgactt aggagaatgt attgctatat
     7141 gtactgctat ggaaaaggaa agcagttatt tatttgttta tggtacagtt agttatttta
     7201 taccttaaaa accaacatac ttaccatttc tattgtttaa aaattactgt ccattttttt
     7261 aaaaatttaa agaatgtagt attttctgag gactggtatg gtacaaaaat gtaatcaaaa
     7321 ttttcagata ctacattttt aaaaagtgag gatggggaaa atatgtatca gcatgaccct
     7381 gatgtagaaa aataaagtct gcaattgaac cttggcaaaa tacattacat aattatttct
     7441 gataaggata aatattagct gtcatttaac tgtcgtgaag aatgaaaaag tgatacattt
     7501 gggcttttta gatcatttgt aactaaattt gtacagaaat tctcctgtga aattaagtaa
     7561 accattttcc agatcttgac tgcattgctg agattttgca gttgaaaaat aatttcaaat
     7621 ttttcaggtt ttttgtatat ttattttttt ctaacaaata tatttaacta taaaattaaa
     7681 attcagtagt taaactcttc ataatgcaga aatggatggc ttgattgtat agaaaaatga
     7741 gtctttcagt tacaccattc ttgaaaataa tagtgtatgt ttgaaatttg tcattaatta
     7801 gttgcttttg aaggcaatct atttgaaata tgtaatttcc tgatgttatc tgcattgtgt
     7861 taagaaaaaa ataataggct ataaaatcaa tttttgtaaa aaaaatgtac agttttaagt
     7921 tgtgtacaga aaaaggaaga gcgtacacag tcttagagtg gaatatacct ctagtacagt
     7981 gtatgtgtta attccaactc aagcaaagaa atgagtattt accatctttc ctataacatg
     8041 taaatgtatt attcctgttc tacagacact gtatatttat gaccttattt ggacattatt
     8101 agacactggt ttcttagtaa agcacagtaa cttggatcag attctttgta accgaaacac
     8161 ttaaacatgt attagtaata ccaatatatt tagattttat atttttataa tttagcttct
     8221 atgcattaca gaagttgaat taagaaattt aagtcatcac catatttttg ttttctttac
     8281 cacttgggaa aataatcctc tttgaggcag taacactatg tatgtactgt taataggcca
     8341 gaaagaaata ttggcagttg tcttaggtaa gcaataaaaa catctccaat ttactgaaag
     8401 ttcagaaata taactcttac atgcatggaa agtgtaaacc aatttaggta tttctaagta
     8461 cccaacccta gtattctctc cctcaactcc aacattctca agtcccagat gaaaagtttc
     8521 agtggagcat tagtttaaat atttagtcta atcagagact cacagccctg ggcaatagat
     8581 acatagcttt ttaaaatttt atcctttgtg acctgctata gtggcattgg actattttct
     8641 gcatgtatta taaactaaag attggcctta tttcaattgt ttttattgca caacttcact
     8701 acaaatttaa agactgctgg cattattata aaactgtctt cctccaatcc aagtaaataa
     8761 gtatgtggaa tatttgtgag ctggattagg taacatctga gacttgggat tgactaaatt
     8821 tagttatcac ttggaaatga attacataag atacagtaat cagagaaaat atctttatgc
     8881 agcatgtatg ttaacatcta gaactgtccc tcatctgcag tgcagtagag gaatcagaca
     8941 ttttaagaaa tgtattcaaa tatttttatc ttatatgtta gcagcattta catgttttca
     9001 gatatgattt gtaatataaa ttaatttctc cagttaacaa tgtttacttc aattctgaca
     9061 ttccatttta tacatatata cctactgtat atctctctca aatgtcagct agaagcaact
     9121 ttttcagttt tctatctaat gaaaacggat atattctgaa tgtttttgtt acctgaaatt
     9181 tatttttgag taggttgaaa ggaccaagat acttgagtga ttaaaggact gtaagtgttc
     9241 tgcttatatg caggagttac atgattttaa aagtcataat aggggaagta gttttatcat
     9301 ctgattactc ctattaacct aaaaagtatt tctaacataa cagattgagc agtggttttg
     9361 ttacacttag gcaagaattt attttcccat taaaatttga aaaatacact tttctttgct
     9421 tctacccttg cagtttccct tagttcccct aatttccaag tgatctgaac taaaattaat
     9481 attacattta ctaagaaact atttttgtta attatactga gcaaagtttg ctccaaatga
     9541 aaagtcatta cgaactatgt atagtaccca agaaaaggtc aaattgagtc agaagactgt
     9601 aagtaccact tttggcgctc cagagataaa ccttgtacac actgcagtag aacagtgagt
     9661 agaaaatatg agctataaat agatattatt tttagttttg atagctctgt gttgaaatac
     9721 ttttttaatg cagataaaaa ttatcaggaa attaacctta tggcacagac caaatactta
     9781 gtgcatggct gagtttctac aattcaagga gccaagaaaa taggttcaag atggaatata
     9841 gtacatgcat ataaggcaga tgcacacatg ctggttactt taagattgtg tgtgttgatt
     9901 agtcacaatg atagactata acatgagaca atacaaagtt acatttttgg accatattaa
     9961 aactgcaaga agacaggggt cttactgaag atcttttaga aaacttaaat cctgtcacag
    10021 gatatttaga catgtgtaga atgtagctca atttttaaaa agtaactgac ctagagggtg
    10081 aaagttgaaa ctgacacatt ttcaaattaa gattatgctt attttgtaca gaaaacaatg
    10141 tttaaacaca agcagatctg ttgtatgtaa taagtaacac agagttttaa aacaaattaa
    10201 ttatttagct ttattgaagt ttgttttttt ccttccgaac ctggagtatc atattataaa
    10261 cagcagtttc acaccagaat agcagtgccc tttctttttg tacatactga ttggaccttt
    10321 ctttactgtt acgtggacac tttctatgtt agttttgatg cataattctt tgaatccttt
    10381 tatacaaact agaatgtatg tgtaagaata cctgtccctg caatgtagta actacaaact
    10441 tatttttaaa taaatagaaa acttgttttt ctaagctatt ttgtagagcc tcatgattta
    10501 ttatttgctt tttaaagttt tttggcatca cataaagcac ttagtacata atctcaacaa
    10561 gctgcttacc aaagtggtca tatggcagat accattatgt aaatgctggt tcattagagt
    10621 ttccagtgcc ctgtgtgttt tgcaaagtgt gaaaactgtc agttaaaaga tatttgggtg
    10681 ccgaaatgta atttaattcc aaaacaactg aaggtcaaaa acttatatgc ctttttatgt
    10741 gtacatttaa taaaacaatt ttattgattt cttaccgtaa gttactgtga tgagtgataa
    10801 atacttcact attcagatac tttcgtaaga gatacatttc agtggaacac tttgcataaa
    10861 tattttctca aaaatgtgca atttctggga aaaaaggaat gatggaaaga aggttattgc
    10921 agttttccta gaaattttgt cagattggca tgcattttta ttgactaaga atcccaattt
    10981 tagcatgaag accattagat atgaatacat aaggccataa catttcaaat taagcacatg
    11041 gagtgatttg taattttgtg ttaatttctc cctaagatgt tttgttaaaa tgattttgta
    11101 tataataaat ttctaagttg aggaaggaag gtaaaaaaaa ttcctgataa ccttttcttt
    11161 atgaagtctg ctaataacaa tacctagtat atacttagaa gaaccagcca agaaaaatta
    11221 cctttcagca accactcttt acttatttct cttttgtaat aataccaatt ttatgaccca
    11281 ggattcccca gtatttaacg gaagtaagat taaagaccaa agcacaaaac ctctgttcct
    11341 tgcaatatag tacttcgtgt ttgtaatcaa tgtgtggtaa atttaacaca tttaccactt
    11401 actgattagt ctttgggaaa agaatggtgg aagtcattca atgaaatacc tttctaccta
    11461 attggttaga catactggca gcacagtgta atgaactggc ctggagttgg gaaatgggaa
    11521 tttgaccttc attcctgaca ggaagtagag gaaccccagc aaagtcgtat ctcagagctt
    11581 cattttcctc atctgtaaag ttttaaaggt tgattaaatt atctgtgagg tctaattctg
    11641 ttcaaatagc cagtggtgat aatctcattt gtatactgtc ctctgtattc atggctcaac
    11701 tttggctttt atatttagtc cctaaataga atacatcatt taatgtgtag cttccagaca
    11761 agctgtgtct tgcggccatg acctgattgt tgttaagtgt gaggttttct gaacccttag
    11821 cagaaggact tttaatgttt ttacaacaca ctattgtatt gctataaatt gtgaattgct
    11881 ttaaaagtac atgtttacta ataaaaagaa gtacaggcac acttta
//
