LOCUS       NM_016436               5879 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens PHD finger protein 20 (PHF20), mRNA.
ACCESSION   NM_016436
VERSION     NM_016436.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Zhang,Z., Wang,L., Zhao,L., Wang,Q., Yang,C., Zhang,M., Wang,B.,
            Jiang,K., Ye,Y., Wang,S. and Shen,Z.
  TITLE     N6-methyladenosine demethylase ALKBH5 suppresses colorectal cancer
            progression potentially by decreasing PHF20 mRNA methylation
  JOURNAL   Clin Transl Med 12 (8), e940 (2022)
   PUBMED   35979628
  REMARK    GeneRIF: N6-methyladenosine demethylase ALKBH5 suppresses
            colorectal cancer progression potentially by decreasing PHF20 mRNA
            methylation.
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Liu,X., Zhang,Z., Kan,S., Lv,Z., Zhou,S., Liu,X., Jing,P. and Xu,W.
  TITLE     PHF20 inhibition promotes apoptosis and cisplatin chemosensitivity
            via the OCT4-p-STAT3-MCL1 signaling pathway in hypopharyngeal
            squamous cell carcinoma
  JOURNAL   Int J Oncol 59 (1) (2021)
   PUBMED   33982773
  REMARK    GeneRIF: PHF20 inhibition promotes apoptosis and cisplatin
            chemosensitivity via the OCT4pSTAT3MCL1 signaling pathway in
            hypopharyngeal squamous cell carcinoma.
REFERENCE   3  (bases 1 to 5879)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5879)
  AUTHORS   Long,W., Zhao,W., Ning,B., Huang,J., Chu,J., Li,L., Ma,Q., Xing,C.,
            Wang,H.Y., Liu,Q. and Wang,R.F.
  TITLE     PHF20 collaborates with PARP1 to promote stemness and
            aggressiveness of neuroblastoma cells through activation of SOX2
            and OCT4
  JOURNAL   J Mol Cell Biol 10 (2), 147-160 (2018)
   PUBMED   29452418
  REMARK    GeneRIF: PHF20 interacts with poly(ADP-ribose) polymerase 1 (PARP1)
            and directly binds to promoter regions of octamer-binding
            transcription factor 4 (OCT4) and sex determining region Y-box 2
            (SOX2) to modulate a histone mark associated with active
            transcription, trimethylation of lysine 4 on histone H3 protein
            subunit (H3K4me3).
REFERENCE   5  (bases 1 to 5879)
  AUTHORS   Klein,B.J., Wang,X., Cui,G., Yuan,C., Botuyan,M.V., Lin,K., Lu,Y.,
            Wang,X., Zhao,Y., Bruns,C.J., Mer,G., Shi,X. and Kutateladze,T.G.
  TITLE     PHF20 Readers Link Methylation of Histone H3K4 and p53 with H4K16
            Acetylation
  JOURNAL   Cell Rep 17 (4), 1158-1170 (2016)
   PUBMED   27760318
  REMARK    GeneRIF: These findings establish a unique PHF20-mediated link
            between MOF histone acetyltransferase (HAT), p53, and dimethylated
            lysine 4 of histone H3, and suggest a model for rapid spreading of
            H4K16ac-enriched open chromatin.
REFERENCE   6  (bases 1 to 5879)
  AUTHORS   Pallasch,C.P., Struss,A.K., Munnia,A., Konig,J., Steudel,W.I.,
            Fischer,U. and Meese,E.
  TITLE     Autoantibodies against GLEA2 and PHF3 in glioblastoma:
            tumor-associated autoantibodies correlated with prolonged survival
  JOURNAL   Int J Cancer 117 (3), 456-459 (2005)
   PUBMED   15906353
  REMARK    GeneRIF: Survival of patients with GLEA2 antibodies was increased
            to 17.4 months and for patients with PHF3 antibodies to 14.7
            months, as compared to 7.2 months for patients without GLEA2 or
            PHF3 antibodies in glioblastoma.
REFERENCE   7  (bases 1 to 5879)
  AUTHORS   Behrends,U., Schneider,I., Rossler,S., Frauenknecht,H., Golbeck,A.,
            Lechner,B., Eigenstetter,G., Zobywalski,C., Muller-Weihrich,S.,
            Graubner,U., Schmid,I., Sackerer,D., Spath,M., Goetz,C., Prantl,F.,
            Asmuss,H.P., Bise,K. and Mautner,J.
  TITLE     Novel tumor antigens identified by autologous antibody screening of
            childhood medulloblastoma cDNA libraries
  JOURNAL   Int J Cancer 106 (2), 244-251 (2003)
   PUBMED   12800201
REFERENCE   8  (bases 1 to 5879)
  AUTHORS   Wang,Y., Han,K.J., Pang,X.W., Vaughan,H.A., Qu,W., Dong,X.Y.,
            Peng,J.R., Zhao,H.T., Rui,J.A., Leng,X.S., Cebon,J., Burgess,A.W.
            and Chen,W.F.
  TITLE     Large scale identification of human hepatocellular
            carcinoma-associated antigens by autoantibodies
  JOURNAL   J Immunol 169 (2), 1102-1109 (2002)
   PUBMED   12097419
REFERENCE   9  (bases 1 to 5879)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   10 (bases 1 to 5879)
  AUTHORS   Fischer,U., Struss,A.K., Hemmer,D., Pallasch,C.P., Steudel,W.I. and
            Meese,E.
  TITLE     Glioma-expressed antigen 2 (GLEA2): a novel protein that can elicit
            immune responses in glioblastoma patients and some controls
  JOURNAL   Clin Exp Immunol 126 (2), 206-213 (2001)
   PUBMED   11703362
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC093405.1, AY027523.1, AL137330.1 and AL109965.34.
            
            On Nov 23, 2018 this sequence version replaced NM_016436.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150178.1, SRR1660803.41886.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374012.8/ ENSP00000363124.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-396               BC093405.1         15-410
            397-3198            AY027523.1         336-3137
            3199-5875           AL137330.1         262-2938
            5876-5879           AL109965.34        60497-60500
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.22-q11.23"
     gene            1..5879
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /note="PHD finger protein 20"
                     /db_xref="GeneID:51230"
                     /db_xref="HGNC:HGNC:16098"
                     /db_xref="MIM:610335"
     exon            1..65
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            66..180
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..3136
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /note="glioma-expressed antigen 2; hepatocellular
                     carcinoma-associated antigen 58; transcription factor TZP;
                     novel zinc finger protein; tudor domain containing 20A"
                     /codon_start=1
                     /product="PHD finger protein 20"
                     /protein_id="NP_057520.2"
                     /db_xref="CCDS:CCDS13268.1"
                     /db_xref="GeneID:51230"
                     /db_xref="HGNC:HGNC:16098"
                     /db_xref="MIM:610335"
                     /translation="MTKHPPNRRGISFEVGAQLEARDRLKNWYPAHIEDIDYEEGKVL
                     IHFKRWNHRYDEWFCWDSPYLRPLEKIQLRKEGLHEEDGSSEFQINEQVLACWSDCRF
                     YPAKVTAVNKDGTYTVKFYDGVVQTVKHIHVKAFSKDQNIVGNARPKETDHKSLSSSP
                     DKREKFKEQRKATVNVKKDKEDKPLKTEKRPKQPDKEGKLICSEKGKVSEKSLPKNEK
                     EDKENISENDREYSGDAQVDKKPENDIVKSPQENLREPKRKRGRPPSIAPTAVDSNSQ
                     TLQPITLELRRRKISKGCEVPLKRPRLDKNSSQEKSKNYSENTDKDLSRRRSSRLSTN
                     GTHEILDPDLVVSDLVDTDPLQDTLSSTKESEEGQLKSALEAGQVSSALTCHSFGDGS
                     GAAGLELNCPSMGENTMKTEPTSPLVELQEISTVEVTNTFKKTDDFGSSNAPAVDLDH
                     KFRCKVVDCLKFFRKAKLLHYHMKYFHGMEKSLEPEESPGKRHVQTRGPSASDKPSQE
                     TLTRKRVSASSPTTKDKEKNKEKKFKEFVRVKPKKKKKKKKKTKPECPCSEEISDTSQ
                     EPSPPKAFAVTRCGSSHKPGVHMSPQLHGPESGHHKGKVKALEEDNLSESSSESFLWS
                     DDEYGQDVDVTTNPDEELDGDDRYDFEVVRCICEVQEENDFMIQCEECQCWQHGVCMG
                     LLEENVPEKYTCYVCQDPPGQRPGFKYWYDKEWLSRGHMHGLAFLEENYSHQNAKKIV
                     ATHQLLGDVQRVIEVLHGLQLKMSILQSREHPDLPLWCQPWKQHSGEGRSHFRNIPVT
                     DTRSKEEAPSYRTLNGAVEKPRPLALPLPRSVEESYITSEHCYQKPRAYYPAVEQKLV
                     VETRGSALDDAVNPLHENGDDSLSPRLGWPLDQDRSKGDSDPKPGSPKVKEYVSKKAL
                     PEEAPARKLLDRGGEGLLSSQHQWQFNLLTHVESLQDEVTHRMDSIEKELDVLESWLD
                     YTGELEPPEPLARLPQLKHCIKQLLMDLGKVQQIALCCST"
     exon            181..352
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            353..437
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            438..517
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            518..905
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1019
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1199
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1379
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1658
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1757
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1922
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2101
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2201
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2397
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2809
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..2993
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..5879
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5857..5862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /note="hexamer: AATAAA"
     polyA_site      5879
                     /gene="PHF20"
                     /gene_synonym="C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP"
                     /note="major polyA site"
ORIGIN      
        1 gcagagcacg cagagtcctt ctgtcggttg gtcctggagc ggcgcagccg gagtggggcc
       61 gtgaggagaa taaaggcagc cccgttgatg actgaaaatg acaaagcatc cacctaacag
      121 acgaggaatc agctttgaag tgggagccca gttggaagcc cgggaccgtt taaaaaactg
      181 gtatccagct cacatagaag acattgacta cgaggaagga aaagtactca tccatttcaa
      241 gcgttggaac catcgttatg atgagtggtt ctgctgggac agtccttatt tacgcccttt
      301 agagaaaata cagctgagga aagagggctt gcatgaagag gatggatctt ctgaatttca
      361 aataaatgag caggtccttg cttgctggtc tgattgtcgt ttttacccgg ccaaagtcac
      421 tgctgttaac aaggatggta cttacactgt gaaattttat gatggagtag ttcagactgt
      481 caaacatatt catgtcaaag ctttttccaa agatcagaat attgtgggta atgctaggcc
      541 taaagaaaca gatcacaaaa gtctttcatc atctcctgat aaacgagaga agtttaaaga
      601 acagagaaaa gcaacagtga atgtgaagaa agacaaagaa gataaaccct taaagacaga
      661 aaagcgaccc aagcagcctg ataaagaagg aaagttaatc tgttctgaaa aggggaaagt
      721 gtcagagaaa agtcttccca agaacgagaa ggaagacaag gaaaacattt ccgaaaatga
      781 cagagagtat tctggagatg cccaagtgga taagaaacct gaaaatgaca ttgtgaagag
      841 tccacaagaa aacttgaggg aacccaaaag aaaacgaggc agaccccctt ccatagctcc
      901 tactgctgtg gattcaaact ctcaaacttt gcaaccaata acattggaac tgagaagaag
      961 gaaaatatca aaaggatgtg aagtcccatt aaaacgtcct cggcttgaca aaaattcatc
     1021 ccaggaaaag tcaaaaaact actcggaaaa cactgacaaa gacttatcga ggagacgttc
     1081 ctccaggctg tccactaatg ggacccatga gatcctagat cctgacttgg ttgtatcaga
     1141 tttggttgat acggatcctt tgcaagacac gttgtctagt accaaggaat ctgaagaagg
     1201 tcagttgaag tctgctttgg aagctggcca ggtctcatct gcactgactt gccactcctt
     1261 tggggatgga tccggggctg caggcttgga gttgaactgc ccatcaatgg gagaaaacac
     1321 gatgaaaaca gaaccgactt ctccccttgt ggaattacaa gagatttcga ctgtggaagt
     1381 aacaaatact tttaagaaaa cagatgattt tgggtcatct aatgcaccag ctgtcgacct
     1441 agaccataag tttagatgca aagttgtgga ctgtttaaaa tttttccgca aagccaaact
     1501 gttgcactat cacatgaagt atttccatgg aatggagaag tcactggagc cagaagagag
     1561 cccgggaaag aggcatgtcc aaaccagggg cccttcagct tcagacaagc ccagccagga
     1621 gaccctgacc aggaagcggg tctctgccag ttccccaact acaaaagaca aggaaaagaa
     1681 taaagagaag aaattcaagg agtttgtgag agtgaagcca aagaagaaaa agaaaaagaa
     1741 aaagaaaacc aaacctgaat gcccctgcag tgaggagatc agtgacacct cccaggaacc
     1801 ttctccaccc aaggcatttg ctgttaccag gtgtgggtcc tcacacaagc caggggtcca
     1861 tatgagcccg cagcttcatg gcccagaatc tggacaccac aaagggaaag tgaaagcatt
     1921 ggaggaggat aatttgagtg agtcctcttc tgagagcttt ctctggagtg atgatgagta
     1981 tggccaagat gtggatgtga ccaccaaccc agatgaggaa cttgatgggg atgaccgcta
     2041 tgacttcgag gtggtccgct gcatctgtga ggtccaggag gaaaatgact tcatgattca
     2101 gtgtgaagag tgccagtgct ggcagcatgg ggtctgcatg ggattactgg aagaaaatgt
     2161 gcccgagaaa tacacctgtt atgtttgcca agaccctcca ggtcagaggc ctggcttcaa
     2221 gtactggtat gacaaggagt ggctgagcag gggacatatg catggcctgg catttctaga
     2281 agagaactac tcccatcaga atgccaagaa gatcgtggcc acccaccagc ttcttggtga
     2341 tgtgcagaga gtgattgagg ttctgcatgg cctgcagctc aagatgagca tcttgcaaag
     2401 ccgggagcat cctgatctgc cgctgtggtg ccagccttgg aaacagcact caggggaggg
     2461 gagatctcat ttcagaaaca tccctgtcac tgacaccagg agcaaggagg aagctccaag
     2521 ctatagaact ttgaacgggg cagtggagaa gcccaggccc ctggccctgc ccctgccgcg
     2581 ttctgtggag gaatcctata tcaccagtga gcattgctac cagaagcccc gcgcctatta
     2641 ccctgccgtg gagcagaagc tggtggtgga gacgaggggc tctgccctcg acgatgcggt
     2701 caaccccctc catgagaacg gcgatgattc cctttccccg cgcctgggct ggcctctaga
     2761 ccaagacagg agcaaggggg acagtgaccc caaacccggc tccccaaagg tgaaggaata
     2821 tgtctccaaa aaggccctac cagaagaagc ccctgctcgg aagctgctgg acagaggtgg
     2881 agaggggctg ctgagctccc agcaccagtg gcagtttaac ctgctgaccc atgtggaatc
     2941 tcttcaggat gaagttacgc acaggatgga ctccattgag aaggagttgg atgtgttgga
     3001 gagctggctg gactacactg gggaactgga gccccctgag ccgctggcca ggcttccgca
     3061 gctcaagcat tgtatcaagc agctgctgat ggacctgggc aaggtgcagc agatcgccct
     3121 ctgctgctca acatgaaact gggcacccaa aactcatggg ggcacaatcc tggggcacct
     3181 gcaggaggag cttcgcatat ttaaataaat aaacctagca tgctgaatgc acgtgacacc
     3241 gactgacttc agggatctgg gccaggagtg tggtggacat tggacaaaga ggccattttg
     3301 gctgcgggag gacactctga tctcgaagcc tgccataaag gtagcaaata gactcttggg
     3361 attcccctct tctgtgcaca tcgttgaatg aagagagtct ttttgcacaa acttcacttg
     3421 aaattgtgcc actgatgata aacggaatga gagccaaaaa agtttagttg gagacagttg
     3481 taaattcaat ttggagttta tttaattgac ttttctatca cgttggggca catgccaact
     3541 ccctggtttc ttcctggcat ggtgtttggg cagcaggcat cattttcctt ttctagcttc
     3601 ataggaatat tgtgagctca ccatgctgtg gaggttggga aagagcagag tcttggctgc
     3661 cctgctttct ccttaggact cttcactttt ctcaccacat ctcttgcatg acttcatggt
     3721 actggggaca agtttgtatg ccttcacccc agagctggct gggttatggc ttttgtagca
     3781 gagcccatac agcctatgga agaactagaa tctcactcac agtaataaga atctaggagg
     3841 aattccaaac cgaagcaggc agggtctgga acccaaagga cagcattttc tacccacttc
     3901 ttaatattga cagcttcccc gttctattta atgtccaaaa atgtttccca aaatttcaaa
     3961 ctctttcact gtaaagattt gttacaaaga atgtggtttg gggaattacc ttattttata
     4021 ttgttgtaaa caaacttcaa attctacatg tgcgactttt ctccttcctg aagggtgttt
     4081 agtagtcagc gttttcagaa ttgttttgtt actatacttt aacattttac atttcctgtt
     4141 tgtattattt tgtgagagca aggtgatcat gctgcttaag gtccaggtac aacctatttg
     4201 taccttttga gacaatattt gtgttacttt tgcaggttac ggttccacat gtaattgcta
     4261 tattttgttt tgtttttcct tactaggcaa agttaaaatg ttccatgctt tgaggagtga
     4321 cccatttcac tactttgttt tcttatcact aaaggcaaaa atcaaagcac agttgtccat
     4381 taacacttat aagttaatta tgggtttatg agtctgtaat gttatatgct gcaaacattt
     4441 actatgtaaa cgtgaagtag ccaataatat ctcaatagta gtaacagtat ctttagcgac
     4501 ctttggaata gttaagcaca ggtcattgtg gacatgaatt caggcctctg tactaaaatc
     4561 tatttcaggg aatgttctgt ctagtgattt gctcaccatt tgatatataa tgaattatag
     4621 gacaagtata agctgatctg ctatagctgt ccatcagaga gaatacacgt ggctataaca
     4681 tctataacaa aacgacgatt cctctacaag aggctgtttc tcactgctaa cgttggtgtt
     4741 tctggcgtgg gaagaaatgc acaggcgtgc atggcatgca cgttcagaca gctgcattgt
     4801 aagagttctg tcatgcagtc tgaaaaggga agaaacagga tggctttctg tagccacacc
     4861 tgtgaggcgt gatgattgtt gtattattag attactgatt tttcttttct gaaaatacat
     4921 ttgagtttta atcacatctg tggaagctgt aacttttaag gtagtcaatt tcatgtcttg
     4981 ttcaacgatg taagcagaaa ctgaattgcc ctaattttgc caactggcca ttattgggat
     5041 tattgtttaa atttttggta aaggaagtaa tctcctttca ttcattgtga cttttgttct
     5101 tagggaagca gagaagactc cccaagttct tataacctca ttgtgcttcc ctaatttaaa
     5161 gccatgttga ggggctccat tcccaattcc tgggtcaagg tgaattaacc ctatgttgga
     5221 gcactggaaa ccgttatttg caaacattgc tgttaccatt tagaaatatg tgcactatca
     5281 gctgggtgca gtggctcacg tctgtaatcc cagcactttg ggaggccgag gcaggtggat
     5341 cacctgaggt caggagttcg agaccagcct ggccaacagg gtgaaacccc gtctctacta
     5401 aaagtacaaa aattagctgg gcgtggtggc ggacgcctgt aatctcagct acttgggagg
     5461 ctgaagcagg agaggtgctg gaacctggga ggcggaggtt gaagtgagcc gagattgcgc
     5521 tattgcactc cagctcgggc gacaactgca agactccatc tcaaaaaaat aaaaataaaa
     5581 gaaaagaaag aaatatgtgc actacctaag ttttgtcttt agaaaaacta tccacctata
     5641 aaaaattacc ttgacaaaaa tagttccggt ttgactaatc attttgtttc tttaagtggt
     5701 aagtgtatgc aaggtggatc cttgatgagc caacattgca ctgtggatac atatctatgt
     5761 ttacgcgcta ttagaacaga aggcgctgta tatagaaatg ttgctttgaa gcaatatttg
     5821 caaaacacgc agacttctgt atctgtattt ggaaaaaata aaacaggttc ttgttgcta
//
