LOCUS       NM_001385109            3993 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens polyhomeotic homolog 2 (PHC2), transcript variant 5,
            mRNA.
ACCESSION   NM_001385109
VERSION     NM_001385109.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Freire-Beneitez,V., Pomella,N., Millner,T.O., Dumas,A.A.,
            Niklison-Chirou,M.V., Maniati,E., Wang,J., Rajeeve,V., Cutillas,P.
            and Marino,S.
  TITLE     Elucidation of the BMI1 interactome identifies novel regulatory
            roles in glioblastoma
  JOURNAL   NAR Cancer 3 (1), zcab009 (2021)
   PUBMED   34316702
  REMARK    Erratum:[NAR Cancer. 2021 May 25;3(2):zcab020. doi:
            10.1093/narcan/zcab020.. PMID: 34319293]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3993)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 3993)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3993)
  AUTHORS   Gray,F., Cho,H.J., Shukla,S., He,S., Harris,A., Boytsov,B.,
            Jaremko,L., Jaremko,M., Demeler,B., Lawlor,E.R., Grembecka,J. and
            Cierpicki,T.
  TITLE     BMI1 regulates PRC1 architecture and activity through homo- and
            hetero-oligomerization
  JOURNAL   Nat Commun 7, 13343 (2016)
   PUBMED   27827373
  REMARK    GeneRIF: Interaction of BMI1 with polyhomeotic protein PHC2 and
            homo-oligomerization via ubiquitin-like domain are necessary for
            H2A ubiquitination activity of PRC1 and for clonogenic potential of
            U2OS cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3993)
  AUTHORS   Tonkin,E., Hagan,D.M., Li,W. and Strachan,T.
  TITLE     Identification and characterisation of novel mammalian homologues
            of Drosophila polyhomeoticpermits new insights into relationships
            between members of the polyhomeotic family
  JOURNAL   Hum Genet 111 (4-5), 435-442 (2002)
   PUBMED   12384788
REFERENCE   7  (bases 1 to 3993)
  AUTHORS   Levine,S.S., Weiss,A., Erdjument-Bromage,H., Shao,Z., Tempst,P. and
            Kingston,R.E.
  TITLE     The core of the polycomb repressive complex is compositionally and
            functionally conserved in flies and humans
  JOURNAL   Mol Cell Biol 22 (17), 6070-6078 (2002)
   PUBMED   12167701
REFERENCE   8  (bases 1 to 3993)
  AUTHORS   Gunther,M., Laithier,M. and Brison,O.
  TITLE     A set of proteins interacting with transcription factor Sp1
            identified in a two-hybrid screening
  JOURNAL   Mol Cell Biochem 210 (1-2), 131-142 (2000)
   PUBMED   10976766
REFERENCE   9  (bases 1 to 3993)
  AUTHORS   Satijn,D.P., Gunster,M.J., van der Vlag,J., Hamer,K.M., Schul,W.,
            Alkema,M.J., Saurin,A.J., Freemont,P.S., van Driel,R. and Otte,A.P.
  TITLE     RING1 is associated with the polycomb group protein complex and
            acts as a transcriptional repressor
  JOURNAL   Mol Cell Biol 17 (7), 4105-4113 (1997)
   PUBMED   9199346
REFERENCE   10 (bases 1 to 3993)
  AUTHORS   Gunster,M.J., Satijn,D.P., Hamer,K.M., den Blaauwen,J.L., de
            Bruijn,D., Alkema,M.J., van Lohuizen,M., van Driel,R. and Otte,A.P.
  TITLE     Identification and characterization of interactions between the
            vertebrate polycomb-group protein BMI1 and human homologs of
            polyhomeotic
  JOURNAL   Mol Cell Biol 17 (4), 2326-2335 (1997)
   PUBMED   9121482
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL138837.12 and AL513327.34.
            
            Summary: In Drosophila melanogaster, the 'Polycomb' group (PcG) of
            genes are part of a cellular memory system that is responsible for
            the stable inheritance of gene activity. PcG proteins form a large
            multimeric, chromatin-associated protein complex. The protein
            encoded by this gene has homology to the Drosophila PcG protein
            'polyhomeotic' (Ph) and is known to heterodimerize with EDR1 and
            colocalize with BMI1 in interphase nuclei of human cells. The
            specific function in human cells has not yet been determined. Two
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853561.571.1,
                                           SRR14038193.4475011.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000683057.1/ ENSP00000507877.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               AL138837.12        42141-42260         c
            121-348             AL513327.34        173196-173423       c
            349-507             AL513327.34        170119-170277       c
            508-585             AL513327.34        168847-168924       c
            586-750             AL513327.34        168251-168415       c
            751-837             AL513327.34        166366-166452       c
            838-1150            AL513327.34        164946-165258       c
            1151-1566           AL513327.34        152668-153083       c
            1567-1732           AL513327.34        152231-152396       c
            1733-1935           AL513327.34        131920-132122       c
            1936-2065           AL513327.34        130105-130234       c
            2066-2180           AL513327.34        129178-129292       c
            2181-2322           AL513327.34        127901-128042       c
            2323-2599           AL513327.34        126700-126976       c
            2600-3993           AL513327.34        121456-122849       c
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p35.1"
     gene            1..3993
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /note="polyhomeotic homolog 2"
                     /db_xref="GeneID:1912"
                     /db_xref="HGNC:HGNC:3183"
                     /db_xref="MIM:602979"
     exon            1..120
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            121..348
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     CDS             175..2754
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /note="isoform e is encoded by transcript variant 5; early
                     development regulator 2 (homolog of polyhomeotic 2);
                     polyhomeotic-like protein 2; early development regulatory
                     protein 2; polyhomeotic-like 2"
                     /codon_start=1
                     /product="polyhomeotic-like protein 2 isoform e"
                     /protein_id="NP_001372038.1"
                     /db_xref="CCDS:CCDS90911.1"
                     /db_xref="GeneID:1912"
                     /db_xref="HGNC:HGNC:3183"
                     /db_xref="MIM:602979"
                     /translation="MENELPVPHTSSSACATSSTSGASSSSGCNNSSSGGSGRPTGPQ
                     ISVYSGIPDRQTVQVIQQALHRQPSTAAQYLQQMYAAQQQHLMLQTAALQQQHLSSAQ
                     LQSLAAVQQASLVSNRQGSTSGSNVSAQAPAQSSSINLAASPAAAQLLNRAQSVNSAA
                     ASGIAQQAVLLGNTSSPALTASQAQMYLRAQMLIFTPTATVATVQPELGTGSPARPPT
                     PAQVQNLTLRTQQTPAAAASGPTPTQPVLPSLALKPTPGGSQPLPTPAQSRNTAQASP
                     AGAKPGIADSVMEPHKKGDGNSSVPGSMEGRAGLSRTVPAVAAHPLIAPAYAQLQPHQ
                     LLPQPSSKHLQPQFVIQQQPQPQQQQPPPQQSRPVLQAEPHPQLASVSPSVALQPSSE
                     AHAMPLGPVTPALPLQCPTANLHKPGGSQQCHPPTPDTGPQNGHPEGVPHTPQRRFQH
                     TSAVILQLQPASPVPQQCVPDDWKEVAPGEKSVPETRSGPSPHQQAIVTAMPGGLPVP
                     TSPNIQPSPAHETGQGIVHALTDLSSPGMTSGNGNSASSIAGTAPQNGENKPPQAIVK
                     PQILTHVIEGFVIQEGAEPFPVGRSSLLVGNLKKKYAQGFLPEKLPQQDHTTTTDSEM
                     EEPYLQESKEEGAPLKLKCELCGRVDFAYKFKRSKRFCSMACAKRYNVGCTKRVGLFH
                     SDRSKLQKAGAATHNRRRASKASLPPLTKDTKKQPTGTVPLSVTAALQLTHSQEDSSR
                     CSDNSSYEEPLSPISASSSTSRRRQGQRDLELPDMHMRDLVGMGHHFLPSEPTKWNVE
                     DVYEFIRSLPGCQEIAEEFRAQEIDGQALLLLKEDHLMSAMNIKLGPALKIYARISML
                     KDS"
     exon            349..507
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            508..585
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            586..750
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            751..837
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1150
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1566
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1732
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1935
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2065
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2180
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2181..2322
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2599
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..3993
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3975..3980
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /note="hexamer: AATAAA"
     polyA_site      3993
                     /gene="PHC2"
                     /gene_synonym="EDR2; HPH2; PH2"
                     /note="major polyA site"
ORIGIN      
        1 ctccgcgggc agcgtccgag cagccagcag ggtgcgcggc gccgagccga gggggcgggc
       61 tgaggggcgg cccccgggag agagcggcca ggtggaggag gagcgcggag gcgaccgcag
      121 catctgcctg cccttctgcc atccgagcgc cctgactgcg ccacactgca ggccatggag
      181 aatgagctgc cagtcccaca tacatctagc agtgcctgtg ccaccagcag taccagcggg
      241 gccagtagca gcagtggctg caacaacagc agcagtggtg gaagtggccg ccccaccggg
      301 ccccagattt ctgtgtacag tggtattcca gaccggcaga ccgtgcaggt gatccagcag
      361 gccctgcaca gacagcccag cacggccgct cagtacctgc agcagatgta cgccgcccag
      421 cagcagcacc tcatgctgca gaccgcggcg ctccagcagc agcacctcag cagcgcccag
      481 ctccagagcc tggcagccgt acagcaggca agcctggtat ccaatagaca aggaagcact
      541 tcaggcagca atgtgtctgc gcaggccccg gcccagtcat cttcgatcaa cctggcagcc
      601 tccccagcag cagcccagct cctcaaccgg gcccagagtg tgaactctgc agcagcctca
      661 ggcatcgctc agcaggctgt gctcttgggc aacacgtctt ccccagccct gactgcaagc
      721 caagcacaga tgtatctgag ggcacagatg ctcatcttca cgcccacggc caccgtcgct
      781 actgtgcagc ctgagctcgg cactggctcc cccgcccggc cccccacccc cgcccaggta
      841 cagaacttga ccctccgaac acagcagaca ccagcggcag cagcctcggg ccccaccccc
      901 actcagcctg tcctgcccag cttggccctg aaacccacgc cgggcggtag ccagcctctg
      961 cctaccccag cacagagcag aaatactgct caggcttccc ctgcaggtgc caagcctggc
     1021 atagctgaca gtgtgatgga gccacacaag aaaggagatg gcaacagcag tgtgccaggg
     1081 agcatggaag gccgggctgg gctcagccgg acggttcctg ctgtggctgc ccaccccctc
     1141 attgcaccag cctatgctca gctgcagcca caccagctcc tcccacagcc atcctcaaag
     1201 cacctgcagc cccaatttgt gatccagcag cagccacagc cacaacagca gcagccgccg
     1261 ccccagcagt cacggcctgt gctccaagct gagccccacc cccagctcgc ctcagtctct
     1321 ccaagcgtgg ccctccagcc cagctcagag gcccatgcca tgccactagg cccggttaca
     1381 cccgccctgc cactccagtg tcccactgcc aacctgcaca agcctggcgg cagtcagcag
     1441 tgtcaccctc ccacacctga tactgggcct cagaatggac atcccgaggg cgtgccccac
     1501 acccctcaac gcaggttcca gcacacttca gctgtcatct tacaactgca gcctgcttca
     1561 ccagtgcccc agcagtgtgt ccctgatgac tggaaagaag tggcaccagg ggagaaaagt
     1621 gtgcctgaga cgcggtctgg cccatcacca catcagcagg ctattgtcac tgccatgcct
     1681 ggtggcctgc ctgtacccac gagccctaac atccagccgt ccccagctca cgagacaggg
     1741 cagggcattg ttcatgcact gaccgacctc agcagccccg gcatgacctc agggaacgga
     1801 aactctgcct ccagcatcgc cggcactgcc ccccagaatg gtgagaataa accaccacag
     1861 gccattgtga aaccccaaat cctgacgcat gttatcgaag ggtttgtgat ccaggagggg
     1921 gcggagcctt tcccggtggg acgctcgtcc ctgctggtgg ggaatctcaa gaagaagtat
     1981 gcacaggggt tcctgcctga gaaacttcca cagcaggatc acaccaccac cactgactcg
     2041 gagatggagg agccctatct gcaagaatcc aaagaggagg gtgctcccct caaactcaag
     2101 tgtgagctct gtggccgggt ggactttgcc tataagttca agcgttccaa gcgcttctgt
     2161 tccatggctt gtgcaaagag gtacaacgtg ggatgcacca aacgggtggg acttttccac
     2221 tcagaccgga gcaagctgca gaaggcagga gctgcgaccc acaaccgccg tcgggccagc
     2281 aaagccagtc tgccaccact taccaaggat accaagaagc agccaacagg cactgtgccc
     2341 ctttcggtta ctgctgcttt gcagctaaca cacagccagg aagactccag ccgttgctca
     2401 gataactcaa gctatgagga acccttgtca cccatctcag ccagctcatc tacttcccgc
     2461 cggcgacaag gccagcggga cctggagctc cccgacatgc atatgcggga cctggtgggc
     2521 atgggacacc acttcctgcc aagtgagccc accaagtgga atgtagaaga cgtctacgaa
     2581 ttcatccgct ctctgccagg ctgccaggag atagcagagg aattccgtgc ccaggaaatc
     2641 gacgggcaag ccctgctgct gctcaaggag gaccacctga tgagcgccat gaacatcaag
     2701 ctggggcccg ccctgaagat ctacgcccgc atcagcatgc tcaaggactc ctagggctgg
     2761 tggcagccag gattctggcc cagggcgcct cctcccgact gagcagagcc agacagacat
     2821 tcctgagggg cccagaaatg gggccggttg gagggcaggg gctctcccta ggggcatagc
     2881 tggtgaggag gtctgggcac ctcctccatg gctctcaggg gcctttcatt tctgtgggag
     2941 gggcagagag gtaggtggca cagaagatgg ggctttatgc ttgtaaatat tgatagcact
     3001 ggcttcctcc aaagtcccaa tactctagcc ccgctctctt cccctctttc tgtcccccat
     3061 tttccagggg gtatatggtc agggctcccc aacctgagtt gggttacttc aagggcagcc
     3121 agcaggcctg gatggaggcc tagaaagccc ttgccttcct tcctcccact tctttctcca
     3181 ggcctggtta actcttccgt tgtcagcttc tcccccttca gcctgtttct gcagcagcca
     3241 gggttctccc ccctacaccc tctgcaggtg gagagagaga agctgggccc agccgggccg
     3301 tgcctgctgg cacagacgcc ttaacgctgt gtgtatgact gtgtgactgt gtgggagcct
     3361 ggactgacag ataggccaag ggctactctc tggcatctcc aggtgttttg tagcaaacag
     3421 ccacttagtg ctttgtcctg gactccactc agcctcagga tggggaatag ccaagaatgg
     3481 cagcctcagc gcagaggcaa ggtcagaaag agacggcgct tcagagtttc ctttccagac
     3541 acccctcccc gcactgtgaa gttcccctga ccgccctcct ggttcacaaa gagcattaag
     3601 aaagctgcgg tggtctgagc aacatagccc aaagggctga gcctcctggc ctgcctgccc
     3661 gcccaccctg ggagtcccag tggtgaggct cagagaactg ctaaggggaa agaacagctg
     3721 gagtttctgt tgatgtgaag aaggcagctc ttggcctccc actcccacac ttctttgcct
     3781 ataaatcttc ctagcagcaa tttgagctac ctgaggagga ggcagggcag aaagggcgag
     3841 ggcctgcctc tgacctgccg tgtcctttgc aggaaggagg taggcacctt tctgagctta
     3901 ttctattccc cacccacacc cccaggcagg gttggaaatg aaggactttt ttaacctttg
     3961 ttttgttttt taaaaataaa tctgtaaaat ctg
//
