LOCUS       NM_080672               2736 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens phosphatase and actin regulator 3 (PHACTR3),
            transcript variant 1, mRNA.
ACCESSION   NM_080672
VERSION     NM_080672.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Bosch,L.J., Oort,F.A., Neerincx,M., Khalid-de Bakker,C.A., Terhaar
            sive Droste,J.S., Melotte,V., Jonkers,D.M., Masclee,A.A.,
            Mongera,S., Grooteclaes,M., Louwagie,J., van Criekinge,W.,
            Coupe,V.M., Mulder,C.J., van Engeland,M., Carvalho,B. and
            Meijer,G.A.
  TITLE     DNA methylation of phosphatase and actin regulator 3 detects
            colorectal cancer in stool and complements FIT
  JOURNAL   Cancer Prev Res (Phila) 5 (3), 464-472 (2012)
   PUBMED   22135045
  REMARK    GeneRIF: DNA methylation of phosphatase and actin regulator 3 is
            associated with colorectal cancer.
REFERENCE   3  (bases 1 to 2736)
  AUTHORS   Farghaian,H., Chen,Y., Fu,A.W., Fu,A.K., Ip,J.P., Ip,N.Y.,
            Turnley,A.M. and Cole,A.R.
  TITLE     Scapinin-induced inhibition of axon elongation is attenuated by
            phosphorylation and translocation to the cytoplasm
  JOURNAL   J Biol Chem 286 (22), 19724-19734 (2011)
   PUBMED   21487013
  REMARK    GeneRIF: Because its expression is highest in relatively plastic
            regions of the adult brain (cortex, hippocampus), scapinin is a new
            regulator of neurite outgrowth and neuroplasticity in the brain.
REFERENCE   4  (bases 1 to 2736)
  AUTHORS   Bankovic,J., Stojsic,J., Jovanovic,D., Andjelkovic,T.,
            Milinkovic,V., Ruzdijic,S. and Tanic,N.
  TITLE     Identification of genes associated with non-small-cell lung cancer
            promotion and progression
  JOURNAL   Lung Cancer 67 (2), 151-159 (2010)
   PUBMED   19473719
  REMARK    GeneRIF: E2F4, PHACTR3, PRAME family member and CDH12 most probably
            play important role in non-small-cell lung cancer geneses
REFERENCE   5  (bases 1 to 2736)
  AUTHORS   Sagara,J., Arata,T. and Taniguchi,S.
  TITLE     Scapinin, the protein phosphatase 1 binding protein, enhances cell
            spreading and motility by interacting with the actin cytoskeleton
  JOURNAL   PLoS One 4 (1), e4247 (2009)
   PUBMED   19158953
  REMARK    GeneRIF: scapinin enhances cell spreading and motility through
            direct interaction with actin and PP1 plays a regulatory role in
            scapinin-induced morphological changes
REFERENCE   6  (bases 1 to 2736)
  AUTHORS   Worch,S., Fiedler,E., Hansmann,I. and Schlote,D.
  TITLE     Genomic organization and expression pattern of scapinin (PHACTR3)
            in mouse and human
  JOURNAL   Cytogenet Genome Res 115 (1), 23-29 (2006)
   PUBMED   16974080
  REMARK    GeneRIF: a highly conserved complex genomic organization with four
            different leader exons; Alternative splicing of exon 5 was found to
            be limited to human vs. mouse; expression seems to occur
            predominantly in the brain
REFERENCE   7  (bases 1 to 2736)
  AUTHORS   Allen,P.B., Greenfield,A.T., Svenningsson,P., Haspeslagh,D.C. and
            Greengard,P.
  TITLE     Phactrs 1-4: A family of protein phosphatase 1 and actin regulatory
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (18), 7187-7192 (2004)
   PUBMED   15107502
REFERENCE   8  (bases 1 to 2736)
  AUTHORS   Sagara,J., Higuchi,T., Hattori,Y., Moriya,M., Sarvotham,H.,
            Shima,H., Shirato,H., Kikuchi,K. and Taniguchi,S.
  TITLE     Scapinin, a putative protein phosphatase-1 regulatory subunit
            associated with the nuclear nonchromatin structure
  JOURNAL   J Biol Chem 278 (46), 45611-45619 (2003)
   PUBMED   12925532
  REMARK    GeneRIF: scapinin is a putative regulatory subunit of PP1 and is
            involved in transformed or immature phenotypes of HL-60 cells
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121908.16, BC108303.1 and
            AB098521.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_080672.4.
            
            Summary: This gene encodes a member of the phosphatase and actin
            regulator protein family. The encoded protein is associated with
            the nuclear scaffold in proliferating cells, and binds to actin and
            the catalytic subunit of protein phosphatase-1, suggesting that it
            functions as a regulatory subunit of protein phosphatase-1.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ311122.2, BC108303.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371015.6/ ENSP00000360054.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AL121908.16        29382-29388
            8-2676              BC108303.1         1-2669
            2677-2736           AB098521.1         2313-2372
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.32-q13.33"
     gene            1..2736
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /note="phosphatase and actin regulator 3"
                     /db_xref="GeneID:116154"
                     /db_xref="HGNC:HGNC:15833"
                     /db_xref="MIM:608725"
     exon            1..593
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     CDS             476..2155
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /note="isoform 1 is encoded by transcript variant 1;
                     scaffold-associated PP1-inhibiting protein; protein
                     phosphatase 1, regulatory subunit 123"
                     /codon_start=1
                     /product="phosphatase and actin regulator 3 isoform 1"
                     /protein_id="NP_542403.1"
                     /db_xref="CCDS:CCDS13480.1"
                     /db_xref="GeneID:116154"
                     /db_xref="HGNC:HGNC:15833"
                     /db_xref="MIM:608725"
                     /translation="MAASEDGSGCLVSRGRSQSDPSVLTDSSATSSADAGENPDEMDQ
                     TPPARPEYLVSGIRTPPVRRNSKLATLGRIFKPWKWRKKKNEKLKQTTSALEKKMAGR
                     QGREELIKKGLLEMMEQDAESKTCNPDGGPRSVQSEPPTPKSETLTSEDAQPGSPLAT
                     GTDQVSLDKPLSSAAHLDDAAKMPSASSGEEADAGSLLPTTNELSQALAGADSLDSPP
                     RPLERSVGQLPSPPLLPTPPPKASSKTTKNVTGQATLFQASSMKSADPSLRGQLSTPT
                     GSPHLTTVHRPLPPSRVIEELHRALATKHRQDSFQGRESKGSPKKRLDVRLSRTSSVE
                     RGKEREEAWSFDGALENKRTAAKESEENKENLIINSELKDDLLLYQDEEALNDSIISG
                     TLPRKCKKELLAVKLRNRPSKQELEDRNIFPRRTDEERQEIRQQIEMKLSKRLSQRPA
                     VEELERRNILKQRNDQTEQEERREIKQRLTRKLNQRPTVDELRDRKILIRFSDYVEVA
                     KAQDYDRRADKPWTRLSAADKAAIRKELNEYKSNEMEVHASSKHLTRFHRP"
     exon            594..755
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            756..833
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            834..1016
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1226
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1401
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1649
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1803
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..1859
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            1860..1921
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2062
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2139
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2736
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2707..2712
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /note="hexamer: ATTAAA"
     polyA_site      2736
                     /gene="PHACTR3"
                     /gene_synonym="C20orf101; H17739; PPP1R123; SCAPIN1;
                     SCAPININ"
                     /note="major polyA site"
ORIGIN      
        1 agattaaagc aattgcaaga gcaaagattc ttccttttcc cttttttcct gggggggtgg
       61 ggggtggggt ggggggaggg agcgccccca gacattccag gacatcaccc cctgccccaa
      121 gcacgcaata aacactgaca agaaaaagtt tttatttcct ggttcaactt tttttttttt
      181 ccctggaata tagactgaag aatgggaata aacacgaata aataacaaag cgaggccgcg
      241 cacgccggga tgcgcctggc tgcagccggc gaggctattg tctccccgcc ctgaagccag
      301 ccccggcgtc tttctccagc tcgtttcctt tcccggcctt tttttttttt tttttttttt
      361 aattttcttt tttaaaaaga cgccccctcc agccccctcg ccggtgacct tggccgcctc
      421 ggatgctctg attccacgcg gctcgctcta acttgccccc gcgccggccg ggcccatggc
      481 cgcgtcggag gacgggagcg gctgcctcgt gtcgcggggc cgctcgcaga gtgaccccag
      541 cgtcctcacc gactcctcgg ccacctcctc cgcggacgcc ggggagaacc cagatgagat
      601 ggaccaaacg cccccggcgc gtcctgaata tctggtctca gggattcgaa ctccccctgt
      661 gaggaggaac agcaaactgg ccaccctggg caggatcttc aaaccctgga aatggaggaa
      721 aaagaaaaac gaaaaactga agcagacaac gtcagcgctg gagaagaaga tggccggcag
      781 gcaaggccga gaggagctca tcaagaaggg gctgctggag atgatggagc aggatgctga
      841 aagcaaaact tgcaaccccg atggaggacc ccgatctgta cagagtgaac cacccactcc
      901 caagtcggag acgctgactt cagaagatgc ccagcccgga agccccttgg ccactgggac
      961 ggaccaggtc tccctggaca agccactgtc ctcagctgcc cacttggacg atgcagccaa
     1021 gatgccttct gcatccagtg gtgaagaagc agacgctggc agcctcctgc ccaccaccaa
     1081 tgagctctcc caagccttag ctggggctga ctccctggac agtcctccca gacctctgga
     1141 gagatccgtg ggccagctcc ccagcccccc actgctgccc actccgccac ccaaggcaag
     1201 ctccaaaacc acaaaaaatg tcacaggcca agccacactc ttccaagcct ccagcatgaa
     1261 gagtgccgac ccttccctcc ggggccagct ctccacaccc acggggtctc cgcatctcac
     1321 cacggtccac cggcctcttc ccccaagccg cgtcattgag gagctgcaca gggcgctggc
     1381 cacgaagcac cgccaggaca gttttcaagg aagagaaagt aaagggtctc caaagaagcg
     1441 gctggatgtc cgtctgtcga gaacgtccag cgtggagcgg ggcaaggaga gggaggaggc
     1501 ttggagcttt gacggggcat tggagaacaa gcgaactgcc gctaaggaat ctgaggagaa
     1561 caaggagaac ctgatcataa attctgaact caaagacgac ttgcttttgt atcaggacga
     1621 ggaggcgctg aacgactcca ttatttctgg aacactgcca cggaaatgca agaaggagct
     1681 cctggccgtg aagctaagga accggccaag caaacaggaa ctagaagacc ggaacatttt
     1741 ccccagaagg actgatgaag aaagacagga gatccggcag cagatcgaga tgaagctttc
     1801 caaacggctg agccaaagac ctgccgtgga agagctggag agaagaaata tcttgaaaca
     1861 aaggaatgat cagacagagc aggaagaaag aagagaaatc aagcaaagat tgacaagaaa
     1921 gcttaatcag agacccactg ttgatgaatt aagagacaga aaaattctga tacgattcag
     1981 tgattacgtg gaagtagcaa aagcgcagga ctatgacagg agggcagaca aaccctggac
     2041 gagactgtca gcagcagata aggcagcaat tcgtaaagaa ttaaatgagt acaaaagtaa
     2101 tgaaatggag gtacatgcat caagcaagca cttgacaaga ttccacaggc catagagatt
     2161 ttcttctgag aagaatttgt gtttaatttt ttgataccaa cactgaacat tcatcaggga
     2221 actttcctga agttcagctc aagactaccc tacctgctgt gtttgtgaga agagtaggat
     2281 cacacacaca ggtgcaatct tgaccacact tacctgcaag aggagtaacc agaggacaca
     2341 cttccttcct tctttggtgt ctgaggagtg tgaactgttg gggtcagtta agacccaaca
     2401 taactctatc agaagaaaac tgttgtttgc ctttcaacct tgttttacag ttctgcagtg
     2461 taatggagga cgggcaacgt gcatgtgcag gctcaccact cccaggcctc tgacatgagg
     2521 gacatgtgac agtgtcattc agtattatgt tcaaaagaca tttttatcct gatcataatt
     2581 aatttgaaaa ctctttaagt tcatgttata caagatgatt tactgtatta tacttttcct
     2641 tttttatata atgtctaaca aaaaatacag ctgcaacatt ttgattcctg ttaattttgt
     2701 tctttaatta aatgactact tattgcagga aattaa
//
