LOCUS       NM_138733               1626 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens phosphoglycerate kinase 2 (PGK2), mRNA.
ACCESSION   NM_138733
VERSION     NM_138733.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1626)
  AUTHORS   Ghanami Gashti,N., Sadighi Gilani,M.A., Jabari,A., Qasemi,M.,
            Feizollahi,N. and Abbasi,M.
  TITLE     The Germ Cell-Specific Markers ZPBP2 and PGK2 in Testicular
            Biopsies Can Predict the Presence as well as the Quality of Sperm
            in Non-obstructive Azoospermia Patients
  JOURNAL   Reprod Sci 28 (5), 1466-1475 (2021)
   PUBMED   33507524
  REMARK    GeneRIF: The Germ Cell-Specific Markers ZPBP2 and PGK2 in
            Testicular Biopsies Can Predict the Presence as well as the Quality
            of Sperm in Non-obstructive Azoospermia Patients.
REFERENCE   4  (bases 1 to 1626)
  AUTHORS   Shen,S., Li,D., Liang,J. and Wang,J.
  TITLE     Testis-specific calcium-binding protein CBP86-IV (CABYR) binds with
            phosphoglycerate kinase 2 in vitro and in vivo experiment
  JOURNAL   Andrologia 51 (7), e13287 (2019)
   PUBMED   30972801
  REMARK    GeneRIF: Study of (Co-IP) and reverse co-immunoprecipitation
            (Co-IP) of CBP86-IV (TSCBP86-IV or CABYR) in human sperm cell
            lysate identified phosphoglycerate kinase 2 (PGK2) as interacting
            partner of CABYR. Protein interactions were further confirmed by
            yeast two-hybrid system.
REFERENCE   5  (bases 1 to 1626)
  AUTHORS   McCarrey,J.R., Kumari,M., Aivaliotis,M.J., Wang,Z., Zhang,P.,
            Marshall,F. and Vandeberg,J.L.
  TITLE     Analysis of the cDNA and encoded protein of the human
            testis-specific PGK-2 gene
  JOURNAL   Dev Genet 19 (4), 321-332 (1996)
   PUBMED   9023984
REFERENCE   6  (bases 1 to 1626)
  AUTHORS   Robinson,M.O. and Simon,M.I.
  TITLE     Determining transcript number using the polymerase chain reaction:
            Pgk-2, mP2, and PGK-2 transgene mRNA levels during spermatogenesis
  JOURNAL   Nucleic Acids Res 19 (7), 1557-1562 (1991)
   PUBMED   2027764
REFERENCE   7  (bases 1 to 1626)
  AUTHORS   Blanche,H., Zoghbi,H.Y., Jabs,E.W., de Gouyon,B., Zunec,R.,
            Dausset,J. and Cann,H.M.
  TITLE     A centromere-based genetic map of the short arm of human chromosome
            6
  JOURNAL   Genomics 9 (3), 420-428 (1991)
   PUBMED   2032717
REFERENCE   8  (bases 1 to 1626)
  AUTHORS   McCarrey,J.R. and Thomas,K.
  TITLE     Human testis-specific PGK gene lacks introns and possesses
            characteristics of a processed gene
  JOURNAL   Nature 326 (6112), 501-505 (1987)
   PUBMED   3453121
REFERENCE   9  (bases 1 to 1626)
  AUTHORS   Michelson,A.M., Bruns,G.A., Morton,C.C. and Orkin,S.H.
  TITLE     The human phosphoglycerate kinase multigene family. HLA-associated
            sequences and an X-linked locus containing a processed pseudogene
            and its functional counterpart
  JOURNAL   J Biol Chem 260 (11), 6982-6992 (1985)
   PUBMED   2987238
REFERENCE   10 (bases 1 to 1626)
  AUTHORS   Chen,S.H., Donahue,R.P. and Scott,C.R.
  TITLE     Characterization of phosphoglycerate kinase from human spermatozoa
  JOURNAL   Fertil Steril 27 (6), 699-701 (1976)
   PUBMED   1278465
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC038843.1.
            
            On May 17, 2019 this sequence version replaced NM_138733.4.
            
            Summary: This gene is intronless, arose via retrotransposition of
            the phosphoglycerate kinase 1 gene, and is expressed specifically
            in the testis. Initially assumed to be a pseudogene, the encoded
            protein is actually a functional phosphoglycerate kinase that
            catalyzes the reversible conversion of 1,3-bisphosphoglycerate to
            3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of
            glycolysis, in the later stages of spermatogenesis.[provided by
            RefSeq, May 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: SRR5189667.135441.1,
                                        SRR5189667.139664.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304801.6/ ENSP00000305995.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1626              BC038843.1         13-1638
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p12.3"
     gene            1..1626
                     /gene="PGK2"
                     /gene_synonym="dJ417L20.2; HEL-S-272; PGKB; PGKPS"
                     /note="phosphoglycerate kinase 2"
                     /db_xref="GeneID:5232"
                     /db_xref="HGNC:HGNC:8898"
                     /db_xref="MIM:172270"
     exon            1..1626
                     /gene="PGK2"
                     /gene_synonym="dJ417L20.2; HEL-S-272; PGKB; PGKPS"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1352
                     /gene="PGK2"
                     /gene_synonym="dJ417L20.2; HEL-S-272; PGKB; PGKPS"
                     /EC_number="2.7.2.3"
                     /note="testicular tissue protein Li 139; epididymis
                     secretory protein Li 272; phosphoglycerate kinase
                     autosomal pseudogene; phosphoglycerate kinase, testis
                     specific"
                     /codon_start=1
                     /product="phosphoglycerate kinase 2"
                     /protein_id="NP_620061.2"
                     /db_xref="CCDS:CCDS4930.1"
                     /db_xref="GeneID:5232"
                     /db_xref="HGNC:HGNC:8898"
                     /db_xref="MIM:172270"
                     /translation="MSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASI
                     PSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGA
                     EVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDV
                     YVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVA
                     DKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKN
                     GVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLI
                     VWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHV
                     STGGGASLELLEGKILPGVEALSNM"
     regulatory      1607..1612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGK2"
                     /gene_synonym="dJ417L20.2; HEL-S-272; PGKB; PGKPS"
                     /note="hexamer: ATTAAA"
     polyA_site      1626
                     /gene="PGK2"
                     /gene_synonym="dJ417L20.2; HEL-S-272; PGKB; PGKPS"
                     /note="major polyA site"
ORIGIN      
        1 aaaggcgtta gaagtcacca ccgacccagc ccctcaacag caagttggtt cttcagcatt
       61 aagatccagg tgtcagccta tgtctttata ttgtcaagat gtctctttct aagaagttga
      121 ctttagacaa actggatgtt agagggaagc gagtcatcat gagagtagac ttcaatgttc
      181 ccatgaagaa gaaccagatt acaaacaacc agaggatcaa ggcttccatc ccaagcatca
      241 agtactgcct ggacaatgga gccaaggcag tagttcttat gagtcatcta ggtcggcctg
      301 atggtgttcc catgcctgac aaatattcct tagcacctgt tgctgttgag ctcaaatcct
      361 tgctgggcaa ggatgttctg ttcctgaagg actgtgtagg cgcagaagtg gagaaagcct
      421 gtgccaaccc agctcctggt tcagtcatcc tgctggagaa cctgcgcttt catgtggagg
      481 aagaagggaa gggccaagat ccctctggaa agaagattaa agctgagcca gataaaatag
      541 aagccttccg agcatcactt tccaagctag gggacgtcta tgtcaatgat gcttttggca
      601 ctgcacaccg cgctcatagt tccatggtgg gagtgaatct gccccataaa gcatccggat
      661 tcttgatgaa gaaggaacta gattactttg ctaaagcctt ggaaaaccca gtgagaccct
      721 ttctggctat acttggtgga gccaaagtgg cagacaagat ccaacttatc aaaaatatgc
      781 tggacaaagt caatgagatg attattggtg gtggaatggc ttataccttc cttaaggtac
      841 tcaacaacat ggagattggt gcttccctgt ttgatgaaga gggagccaag atcgttaaag
      901 atatcatggc caaagcacaa aagaatggtg taaggattac ttttcctgtt gattttgtta
      961 ctggggacaa gtttgacgag aacgctcagg ttggaaaagc cactgtagca tctggcatat
     1021 ctcctggctg gatgggtttg gactgtggtc ctgagagcaa caagaatcat gctcaagttg
     1081 tggctcaagc aaggctaatt gtttggaatg ggccgttagg agtatttgaa tgggatgcct
     1141 ttgctaaggg aaccaaagcc ctcatggatg aaattgtgaa agccacttcc aagggctgca
     1201 tcactgttat agggggtgga gacactgcta cttgctgtgc caaatggaac actgaagata
     1261 aagtcagcca tgtcagcact ggaggcggtg ccagtctaga gcttctggaa ggtaaaatcc
     1321 ttcctggagt agaggccctc agcaacatgt agttaatata gtgttacttc cttctgtttt
     1381 ctgtccatgg cccttaagtc agcttaatgc ttttacatct cgatgtgact tttgttaaaa
     1441 tctactccta gatcaagacc tatgtaatgg acaagcagca ggccatcagg aactcttaat
     1501 atcagcacag caattcattt tagtttggtc acgcatttgc ctgttcaagt tctcatttga
     1561 acttcaccat tgtgctatct agggaggaca tattcttaag ttgcctatta aagaaagtga
     1621 gctgaa
//
