LOCUS       NM_001258311           10914 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens piggyBac transposable element derived 5 (PGBD5), mRNA.
ACCESSION   NM_001258311
VERSION     NM_001258311.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Luo,P., Yang,J., Jian,L., Dong,J., Yin,S., Luo,C. and Zhou,S.
  TITLE     Knockdown of PGBD5 inhibits the malignant progression of glioma
            through upregulation of the PPAR pathway
  JOURNAL   Int J Oncol 64 (5) (2024)
   PUBMED   38577941
  REMARK    GeneRIF: Knockdown of PGBD5 inhibits the malignant progression of
            glioma through upregulation of the PPAR pathway.
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Yamada,M., Keller,R.R., Gutierrez,R.L., Cameron,D., Suzuki,H.,
            Sanghrajka,R., Vaynshteyn,J., Gerwin,J., Maura,F., Hooper,W.,
            Shah,M., Robine,N., Demarest,P., Bayin,N.S., Zapater,L.J., Reed,C.,
            Hebert,S., Masilionis,I., Chaligne,R., Socci,N.D., Taylor,M.D.,
            Kleinman,C.L., Joyner,A.L., Raju,G.P. and Kentsis,A.
  TITLE     Childhood cancer mutagenesis caused by transposase-derived PGBD5
  JOURNAL   Sci Adv 10 (12), eadn4649 (2024)
   PUBMED   38517960
  REMARK    GeneRIF: Childhood cancer mutagenesis caused by transposase-derived
            PGBD5.
REFERENCE   3  (bases 1 to 10914)
  AUTHORS   Beckermann,T.M., Luo,W., Wilson,C.M., Veach,R.A. and Wilson,M.H.
  TITLE     Cognate restriction of transposition by piggyBac-like proteins
  JOURNAL   Nucleic Acids Res 49 (14), 8135-8144 (2021)
   PUBMED   34232995
  REMARK    GeneRIF: Cognate restriction of transposition by piggyBac-like
            proteins.
REFERENCE   4  (bases 1 to 10914)
  AUTHORS   Helou,L., Beauclair,L., Dardente,H., Arensburger,P., Buisine,N.,
            Jaszczyszyn,Y., Guillou,F., Lecomte,T., Kentsis,A. and Bigot,Y.
  TITLE     The C-terminal Domain of piggyBac Transposase Is Not Required for
            DNA Transposition
  JOURNAL   J Mol Biol 433 (7), 166805 (2021)
   PUBMED   33450253
  REMARK    GeneRIF: The C-terminal Domain of piggyBac Transposase Is Not
            Required for DNA Transposition.
            Erratum:[J Mol Biol. 2021 Jul 23;433(15):167089. doi:
            10.1016/j.jmb.2021.167089.. PMID: 34127257]
REFERENCE   5  (bases 1 to 10914)
  AUTHORS   Henssen,A.G., Koche,R., Zhuang,J., Jiang,E., Reed,C., Eisenberg,A.,
            Still,E., MacArthur,I.C., Rodriguez-Fos,E., Gonzalez,S.,
            Puiggros,M., Blackford,A.N., Mason,C.E., de Stanchina,E., Gonen,M.,
            Emde,A.K., Shah,M., Arora,K., Reeves,C., Socci,N.D., Perlman,E.,
            Antonescu,C.R., Roberts,C.W.M., Steen,H., Mullen,E., Jackson,S.P.,
            Torrents,D., Weng,Z., Armstrong,S.A. and Kentsis,A.
  TITLE     PGBD5 promotes site-specific oncogenic mutations in human tumors
  JOURNAL   Nat Genet 49 (7), 1005-1014 (2017)
   PUBMED   28504702
  REMARK    GeneRIF: Previously undefined genomic rearrangements in rhabdoid
            tumors involved PGBD5-specific signal (PSS) sequences at their
            breakpoints and recurrently inactivated tumor-suppressor genes.
            PGBD5 physically associated with genomic PSS sequences mediating
            PGBD5-induced DNA rearrangements in rhabdoid tumor cells. In
            immortalized cells, PGBD5 sufficed for transformation via specific
            catalytic residues in the transposase domain.
            Erratum:[Nat Genet. 2017 Sep 27;49(10):1558. doi:
            10.1038/ng1017-1558b.. PMID: 28951624]
REFERENCE   6  (bases 1 to 10914)
  AUTHORS   Henssen,A.G., Jiang,E., Zhuang,J., Pinello,L., Socci,N.D.,
            Koche,R., Gonen,M., Villasante,C.M., Armstrong,S.A., Bauer,D.E.,
            Weng,Z. and Kentsis,A.
  TITLE     Forward genetic screen of human transposase genomic rearrangements
  JOURNAL   BMC Genomics 17, 548 (2016)
   PUBMED   27491780
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 10914)
  AUTHORS   Henssen,A.G., Henaff,E., Jiang,E., Eisenberg,A.R., Carson,J.R.,
            Villasante,C.M., Ray,M., Still,E., Burns,M., Gandara,J.,
            Feschotte,C., Mason,C.E. and Kentsis,A.
  TITLE     Genomic DNA transposition induced by human PGBD5
  JOURNAL   Elife 4, e10565 (2015)
   PUBMED   26406119
  REMARK    GeneRIF: DNA transposition catalyzed by PGBD5 in human cells occurs
            genome-wide, with precise transposon excision and preference for
            insertion at TTAA sites.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 10914)
  AUTHORS   Anney,R.J., Lasky-Su,J., O'Dushlaine,C., Kenny,E., Neale,B.M.,
            Mulligan,A., Franke,B., Zhou,K., Chen,W., Christiansen,H.,
            Arias-Vasquez,A., Banaschewski,T., Buitelaar,J., Ebstein,R.,
            Miranda,A., Mulas,F., Oades,R.D., Roeyers,H., Rothenberger,A.,
            Sergeant,J., Sonuga-Barke,E., Steinhausen,H., Asherson,P.,
            Faraone,S.V. and Gill,M.
  TITLE     Conduct disorder and ADHD: evaluation of conduct problems as a
            categorical and quantitative trait in the international multicentre
            ADHD genetics study
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 147B (8), 1369-1378 (2008)
   PUBMED   18951430
REFERENCE   9  (bases 1 to 10914)
  AUTHORS   Sarkar,A., Sim,C., Hong,Y.S., Hogan,J.R., Fraser,M.J.,
            Robertson,H.M. and Collins,F.H.
  TITLE     Molecular evolutionary analysis of the widespread piggyBac
            transposon family and related 'domesticated' sequences
  JOURNAL   Mol Genet Genomics 270 (2), 173-180 (2003)
   PUBMED   12955498
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL133516.8, CX757968.1,
            BC036865.1, BC013901.2 and AL691479.15.
            
            On Nov 23, 2018 this sequence version replaced NM_001258311.1.
            
            Summary: The piggyBac family of proteins, found in diverse animals,
            are transposases related to the transposase of the canonical
            piggyBac transposon from the moth, Trichoplusia ni. This family
            also includes genes in several genomes, including human, that
            appear to have been derived from the piggyBac transposons. This
            gene belongs to the subfamily of piggyBac transposable element
            derived (PGBD) genes. The PGBD proteins appear to be novel, with no
            obvious relationship to other transposases, or other known protein
            families. [provided by RefSeq, May 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The genomic coordinates used for
            the transcript record were based on RNA-Seq and homologous
            transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: KF670820.1, SRR1803615.26837.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000391860.7/ ENSP00000375733.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-603               AL133516.8         58624-59226
            604-1183            CX757968.1         33-612
            1184-1759           BC036865.1         207-782
            1760-3399           BC013901.2         165-1804
            3400-3758           BC036865.1         2423-2781
            3759-10914          AL691479.15        9742-16897          c
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q42.13"
     gene            1..10914
                     /gene="PGBD5"
                     /note="piggyBac transposable element derived 5"
                     /db_xref="GeneID:79605"
                     /db_xref="HGNC:HGNC:19405"
                     /db_xref="MIM:616791"
     exon            1..735
                     /gene="PGBD5"
                     /inference="alignment:Splign:2.1.0"
     CDS             405..1979
                     /gene="PGBD5"
                     /note="piggyBac domain related protein 5; piggyBac
                     transposase 5"
                     /codon_start=1
                     /product="piggyBac transposable element-derived protein 5"
                     /protein_id="NP_001245240.1"
                     /db_xref="CCDS:CCDS81430.1"
                     /db_xref="GeneID:79605"
                     /db_xref="HGNC:HGNC:19405"
                     /db_xref="MIM:616791"
                     /translation="MAEGGGGARRRAPALLEAARARYESLHISDDVFGESGPDSGGNP
                     FYSTSAASRSSSAASSDDEREPPGPPGAAPPPPRAPDAQEPEEDEAGAGWSAALRDRP
                     PPRFEDTGGPTRKMPPSASAVDFFQLFVPDNVLKNMVVQTNMYAKKFQERFGSDGAWV
                     EVTLTEMKAFLGYMISTSISHCESVLSIWSGGFYSNRSLALVMSQARFEKILKYFHVV
                     AFRSSQTTHGLYKVQPFLDSLQNSFDSAFRPSQTQVLHEPLIDEDPVFIATCTERELR
                     KRKKRKFSLWVRQCSSTGFIIQIYVHLKEGGGPDGLDALKNKPQLHSMVARSLCRNAA
                     GKNYIIFTGPSITSLTLFEEFEKQGIYCCGLLRARKSDCTGLPLSMLTNPATPPARGQ
                     YQIKMKGNMSLICWYNKGHFRFLTNAYSPVQQGVIIKRKSGEIPCPLAVEAFAAHLSY
                     ICRYDDKYSKYFISHKPNKTWQQVFWFAISIAINNAYILYKMSDAYHVKRYSRAQFGE
                     RLVRELLGLEDASPTH"
     exon            736..1163
                     /gene="PGBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1298
                     /gene="PGBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1479
                     /gene="PGBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1677
                     /gene="PGBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1783
                     /gene="PGBD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..10914
                     /gene="PGBD5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3729..3734
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGBD5"
                     /note="hexamer: AATAAA"
     polyA_site      3758
                     /gene="PGBD5"
                     /note="major polyA site"
     regulatory      10895..10900
                     /regulatory_class="polyA_signal_sequence"
                     /gene="PGBD5"
                     /note="hexamer: AATACA"
     polyA_site      10914
                     /gene="PGBD5"
ORIGIN      
        1 ggcagccgag cgccggaggc cgtggagagg gcgggcaggg aggcggcggg cgccgaggag
       61 acgcagcggc ggcggtggtg gcggtggtgg cggcggtggc ggcggcggcg cctggtctcg
      121 gccgcagcct ctgcgtcccg cccgctctgg gtcgcccctg ccagccccgc gcgcggctcg
      181 cctcccgccg cggcccgcag cctgcggcgc ccgagctccg agggcgcttc cccgggcgct
      241 gggcccgagg cggccaagac ggcgggccgg ccgggcacgg ggcggcgggc gctgtgctgc
      301 gggctgcgct ggtggggggc ggcgggccgc ggcgcgggcc cagggcccgg cgtgtgcggc
      361 tgggaggcac tgtgggggcg ggcgcgcggg cggcggccgg ggccatggcc gagggcggcg
      421 ggggcgcgcg gaggagggcg ccggcgctgc tcgaggctgc ccgcgcgcgc tacgagagcc
      481 tgcacatctc ggacgacgtg ttcggcgagt ccggcccgga cagcggcggg aaccccttct
      541 acagcacctc ggccgcctcg cgctcctcct cggccgcctc ctcggacgac gagcgcgagc
      601 ccccgggacc cccaggggcc gccccgccac cgccccgcgc cccggacgca caggagccgg
      661 aggaggacga ggccggcgcg ggctggagcg cagcgctgcg ggaccgcccg cccccgcgct
      721 tcgaggatac cggcggtccc acccgaaaga tgccccccag cgccagtgcc gtggacttct
      781 tccagctctt tgtcccagac aacgtcctca agaacatggt ggtgcagaca aacatgtatg
      841 ccaagaagtt ccaggagcgg tttgggagcg acggagcctg ggtggaggtg acgctgacgg
      901 agatgaaggc gttcctgggc tacatgatct ccaccagcat ctcccactgc gagtccgtcc
      961 tcagcatctg gagcggaggc ttctacagca accgcagcct cgccctcgtc atgagccagg
     1021 cccgcttcga gaagatcctc aagtacttcc acgtcgtggc cttccgctcc agccagacca
     1081 cgcacgggct ctacaaggtc cagcccttcc tcgactccct gcagaacagc ttcgactctg
     1141 ccttcaggcc ttcccaaacc caggtgctac atgaacccct gatcgatgag gatcctgtat
     1201 tcattgccac gtgcacagag cgggagctgc gaaagaggaa aaagcggaaa ttcagcctct
     1261 gggtcagaca atgttcttcc actggcttca tcatccagat ttatgtccac ctgaaggaag
     1321 gtgggggccc agatggcctg gatgcgctga agaataagcc ccagctccac agcatggtgg
     1381 ccaggagcct gtgccggaac gcggcaggca agaactacat cattttcacg gggcccagca
     1441 tcaccagcct gacgctgttt gaagagtttg agaagcaagg gatttactgc tgcggcttgc
     1501 tccgcgcgcg gaagagtgac tgcaccggcc tcccactgtc catgctgacc aacccagcca
     1561 cacccccggc ccggggccag taccaaatca agatgaaggg gaacatgtcc ttgatctgct
     1621 ggtacaacaa aggacacttc cgcttcctga ccaacgccta ctccccggtg cagcagggag
     1681 tcatcatcaa aaggaagagt ggggagatcc catgcccctt ggccgtggag gcgtttgccg
     1741 ctcacctgag ctacatctgc agatacgatg acaaatacag caagtatttc atttctcata
     1801 aaccaaacaa gacctggcag caggtgttct ggttcgccat cagcatcgcc atcaacaatg
     1861 cctacatcct gtacaaaatg tcagacgcct accacgtgaa gaggtacagc cgggcgcagt
     1921 ttggagagag actcgtcaga gagctgctgg gcttggagga tgcctctccg acccactgat
     1981 gctgggggcg caggactcgg tcaagggagg ggcaagagga ggaggagagc ctgccgttcc
     2041 aacttgccca tcagagaccc ggacacggcc tggtgtgtgg cttgctgcct gggagggatg
     2101 cacagggcct ctggagggac aggatggacc tggtcagagg acggttgctg tcctcatttg
     2161 cattccaaga agagcatgtc ctccctcgag aaacagtgcc gccggtgtga tgagcactta
     2221 cacccacgtt ctcaagggca gattctctca tgacatccgt ggagcttgcg aggcagcgtg
     2281 gactggtgac tgtgaaggaa ggcccccgtg gtagaatgag ctggagcacg ctctaagaga
     2341 gatgcctgct tcctaaagat ctacagcaat ctgggacgtg gttcaagttc aagacttgaa
     2401 ggaagcaaag acgccctgca tggttacaat ggctcaggtg tcaggggagg ccggagggtt
     2461 ttccagcatt tgcctcatgc cagcaccttt gaaccggtct cttagaagaa gacacacatc
     2521 ctgggtgtac agtggtgaaa tggggagtgg gtgcccattc tgaaaaacga ggcattcctg
     2581 ctcattccct ctgcttagct ggtgggcagg ggagagaggg aaatgccaaa aacttggagt
     2641 gaaggatgat gctatttttt atttttaaat atatcttcag gttattttct tactgttgct
     2701 tcagatctaa tgtaaaaggc agatgtcccc tcctctccac ccccgacgct gaccccggcc
     2761 tcagtcacgg ctctttgcat gatcacagtt ctgtgttctg gcctgtggca gggccgggaa
     2821 gggccgctgg cttccgaaca gacgtggttg ctctccacga ggcgcatggg gagcccgcgg
     2881 gccctaagct ttgtcgcaga tgtcatcatt ggcagaatta cttgtcttga aaaataagta
     2941 gcattgctga aacacacaac cgaattctct acgatggcca tttgctcatt gtctttcctc
     3001 tgtgtgtagt gagtgaccct ggcagtgttt gcctgctcag agtggcccct cagaacaaca
     3061 gggctggcct tggaaaaacc ccaaaacagg actgtggtga caactctggt caggtgtgat
     3121 ttgacatgag ggccggaggc ggttgctgac ggcaggactg gagaggctgc gtgcccggca
     3181 ctggcagcga ggctcgtgtg tcccccaggc agatctgggc actttcccaa cccaggttta
     3241 tgcgtctcca gggaagcctc ggtgccagag tggtgggcag atctgaccat ccccacagac
     3301 cagaaacaag gaatttctgg gattacccag tcccccttca acccagttga tgtaaccacc
     3361 tcatttttta caaatacaga atctattcta ctcaggctat gggcctcgtc ctcactcagt
     3421 tattgcgagt gttgctgtcc gcatgctccg ggccccacgt ggctcctgtg ctctagatca
     3481 tggtgactcc cccgccctgt ggttggaatc gatgccacgg attgcaggcc aaatttcaga
     3541 tcgtgtttcc aaacaccctt gctgtgccct ttaatgggat tgaaagcact tttaccacat
     3601 ggagaaatat atttttaatt tgtgatgctt ttctacaagg tccactattt ctgagtttaa
     3661 tgtgtttcca acacttaagg agactctaat gaaagctgat gaattttctt ttctgtccaa
     3721 acaagtaaaa taaaaataaa agtctattta gatgttgatt ctccgttaat acgtgaattc
     3781 gtttaaaagg tcagggaaca ggcggggtgc ggtggctcat gcctgtaatc ccagcacttt
     3841 tggaggctga agcgggcgga tcgcttgagt ccaggagttt gagaccagca ttggcaacat
     3901 ggcgaaagcc catttctaca aaaaatacaa gaattaggtg tggtggcaca tgcctgcagt
     3961 cccagctact tgggaggctg aggtgggagg atctcttgag ccactgcact ccagcctggg
     4021 tgacagagcc agaccctgta tcaagaaaaa aaacaaggtg aaggaacaaa aatacaggct
     4081 actcttttaa atacttgatt ttttttgtta tataatctgg tgtgtatgat attaactgtt
     4141 cttggtgtct caaaccactg agcagtttaa aaaagaaatg tttttgagtt tggctaatag
     4201 aaatacctct tgatgttgca gttgtatgag tgcagttgta tttgaggtgc ctgtttcatt
     4261 gaagacacta gcaggcctgc cttccggcct ggtggttttc cctcttttct tctagtttgt
     4321 gtcgtgtgtc attccagatg agcaggaaga ggtgctgttc tgacaggcag tgggtggctg
     4381 cattccccag tcatgagaga ataattgtaa cattgtaaca ctctgtttct aaaatggcag
     4441 ccacctgttg tctgatacat gatgggaaaa ggcagctgtt gagccgggtc taggggaggc
     4501 ttcagtggcc acttcaggtc tctggaggaa tgctgttgac tgagaagagt ttctcttctc
     4561 catccagaag gacagaatag catgtaggac ggactgacag aacctccagg gacctggacc
     4621 ttgccttaga gatgtgattc aaagtggctt tttggagtga ctctagttga agtgcatctg
     4681 gttccagtat ctccctgggt tcaacagccc tcaggttgat tcttgattgg ctttggagta
     4741 gctggccatc cctggggatg aggaaagagc atcgtaagtg gtcttatgac agagggagcc
     4801 agcatggccc tggctgagta tatggggctg gcaagcacca ctgcacacat ggcaagtgag
     4861 agcccattcc actcagatcc agccactaag ccaagccgat cccctgaggg atgtctctgc
     4921 tgcaggctct ggaaaaaaaa gatggagcaa tgagcttccc agccttatgc aaaacaaaac
     4981 acccacaaaa acttccaagc ttctccaaag agaatctgca tttccctgca actcacaggg
     5041 aattgaacct gcactgagct tcacggagtt tatgagacct tcccattact gcagttgtgt
     5101 tgagcgtccc gtgaggtcct tcttaggcaa atttaacttc attgagtgaa tgtctgtttc
     5161 tgtttggtgc ctacttgatc aggttccata ttcgactttc attgtctttc cccacactct
     5221 gctccctaaa gagcctcgat tttggccagg tgcggtggct cacacctgta atcccagcac
     5281 tttgggaggc cgaggcaggc ggatcacaag gccaggagat tgagaccagc ctggccaata
     5341 tggtgaaacc ccatctctac taaaaataca aaaattagct gggtgtggtg gtggacacct
     5401 gtagtcccag ctactcggga ggctaagcag gagaatatct tgaatccggg aagcagaggc
     5461 tgcagtgagc caagatcgca ccactgcact ccagcctggg tgacagagcg agactccaac
     5521 ccccaccccg ccacaaaaaa aaaaaaaaaa aaaaagcctc gagtttgaac aacgtcttcc
     5581 cgtggtccat gggacattat actagggaca tctttcaaaa gtcaggcaag tcatatggaa
     5641 aagtcttggt ggctgggtca ttctgagcaa acacatccag tactgactaa atcagatgag
     5701 gcttggtcac caaaattgag ttaactctgc tgcgtgcaag aagcaagaag attgcaggtc
     5761 cccaggacag agcctggaag gttctgaatc cttcctgtgt ggcccgtgtg tcaccatcct
     5821 ccccagggac ccaggctcag cccccccaac agtgaggggg ggtcaccaag catcaggctg
     5881 agaggaagca gggcaaaccc gggagggcca tggggccttg caactacgca agcctcacac
     5941 attctcctcc agtctgcatt catggcaaaa aaaaaacatt tccccacttg cctgggattg
     6001 gggcgagaac aagtcaacga cactgatggg ggagaaaggt gaccttgggt gtggtacctc
     6061 ccagatctgt ctcctgcatc ctcgcaggtg ctgtgtttga aaggtatgac agcggtggct
     6121 aacaggcagg cacacttcca ggcggcttgg ggtggccctt ctgccccaga gccttactcc
     6181 tcacagggat ccctgtgggt taggcatgga ctgtcaccac gcccagatgg agtggtgcaa
     6241 tggttcgccc aggtaagcag acccgggttt gcctgacgca ggaggccgcg tcctaactgc
     6301 tgtgggcact ggctgtcctg attcatcacg aggacggggg attttatccc tgacttacct
     6361 gacaactttg tgggggattt tctcctttcc atatccaatc ctctctgctg atccgaatac
     6421 tgaaaacaat cattatgaga tccgggacat caccctcccc ctgggtcctt ttcttgtcct
     6481 agatggagat gggtgttttg gagcttaaca actactgaag ctgaagttag cctggtaggg
     6541 agccactatt ctggggggca tttggacagg gcaagccctt ggccacacca gaccctgagc
     6601 cagaaagaac catagcgatt tcctcaccca gatggcccca gctcacagta tggggagagt
     6661 gggatggggt gggggagtgg agccagctgt ggggtctcat cagagtgccc tgggatctag
     6721 gccagaggcg ccgtcttcac ccacatgcca gctttgtggt tctggtccct cttccttaag
     6781 cgagggcttg ccaagctttg gctgtggaaa gccttagcct tgtgtagtgg gagttccaaa
     6841 cgaaagaagt ccccagaagt tcacaaggta gtggccaggg aaagtgtccc cagtgccccc
     6901 tccccaccgg tggcctctgc agcttgcttt ggaaaacagt tctgtctgtg aagaaatctc
     6961 tcctgttacg gccctgtgtc agtcaatgcg ggcttggctg tctgcccaaa taccttccgt
     7021 tcttatcagg aacatgactg aggcttctca cttttgactc catcacttaa tccctttttc
     7081 tccgtggctt ctgtgtgtct catcttcgag aatatttctc agccttcttt gcaggtatgt
     7141 tattctctcc caataagaaa taaagagtat caggtaccaa agtggcactt agagctgttg
     7201 ccataaaata gcaccagaga gtaagcatgc cctctggcca cgcagctcgc tgccatttag
     7261 ccatctctgt gtccctccct ccctggggtg cccagtgcgc accccagttc tccagcagcc
     7321 caggcacatt ctcctagctc tgacccctgt ctggtggggg ccgggcagcc tctcttgagt
     7381 gtagggcggt ggtgctggcg ctggtggcag ctctgtgtga actgtgttcc ggggagggtg
     7441 gggcaccttc acccttccgt gtatacccat gtatacacgg tcgtaatgag gagactcctg
     7501 cgggagactt gattgtgcgc tccatgtcac tagctactct acaagcagat actattcttc
     7561 aggttttttc cttagttgcc tggaagcttg accttgtgtg catgtatttg gagatgaaaa
     7621 cagaaagggc ggggaggaca tcggcttgtg tgaaagacct aggaagacga tggaggagtg
     7681 tgtgcggctt ccctcacgtg tcaagcctcc tggctccctc aggtggaagg ggcactttat
     7741 ggccaaggaa attcagtgag gagtgaaggg tgaggctgag cccgccagat aaatcagcct
     7801 cggccctccc ctccccaagg gtcagccccc ggcagcgcac ctctgttctt ggtgcgtctc
     7861 gctagtcttt gattcattct tctgccgtct tcacccaagc ctcgctggac taaagagtga
     7921 cagttaatgc acatggagca tttctgttcc agcaggtcac gtctccctgt atggttcctt
     7981 tgctgagtcc tcagcattgc cctgggagct gacaaccatc ggtccccctt ttgtgatggg
     8041 aaagtgacac tctgagagcc gtggtgactc tacagggtgt ggagccaggt tgaccctcac
     8101 tctgtctgct ccagggcttc ctggttctca gccactgctg cttctgaact gctacaggcc
     8161 aggcattgtg ctaagaactg ggcacgtgtt accccaacgt ggtctgtgcc cttgagggtg
     8221 ccatgactga aacacgttgg gggcacagag caggctgatt ctccgccgaa agaaggctcc
     8281 agccagaggc cgagaccccg tgtgggggag gtggcagtca ccattgctgc tggactcagc
     8341 aacccccaga ccctccacct tgcttggcag gcatcacatg cacctggctt agcacatccc
     8401 tgcaaacaca agagtctgag cctcctcaag aggctgcagt cacactgcat ttgaccagga
     8461 cttgccaacc tgcaacttgc cctgagagcc aggtatgcgt gtgtgtatct ggggagcacg
     8521 ccccaaacat gagcagggac ggtgttccca gctatccgag aggtcatgcc caagccaaaa
     8581 ggaaggctct agctcttaaa catgaataag tttattcctt tcatttccac agtgtcttat
     8641 gcttgcttct gttttctcca aggaagtatg gtgcccacct actcctccag cctgacgatc
     8701 catgtgaggg ttccttgttc ccagcccaat tctcatgtcc cacctttctc cactaagaaa
     8761 cagccaaatt ttggcaagag tcgtggtagg aaaaaaaaac aataattggg cagatgagga
     8821 tttttcactt tttgactagt tctttctcta gactttcctg tctttttaaa acttctagtt
     8881 tcccccttga gcgctccctc ccagtgggta gaccacggaa ggaatgaaca ggggatggaa
     8941 gcaggggatg cagtccctat tatttcaata gattggaaag atgggcccag aacaattgcg
     9001 tacggtgttc agtgtaaatt gaagatctgg agttgcagga ttgttgaggc aatttttagt
     9061 tgctttgctc catctaaaca caaggccata ggatagtgtg actttgtagc ttcatcaccg
     9121 tatccacatc agaagtacaa tgtccactta atacatatat acacatatgt acgtatacac
     9181 atatacacat gtgtacatat gtatgtatac acatgcacat gtgtacgtat gtatgtatac
     9241 acatacatat atgtgtacac atatacatat gtatgtatac acatagatat atgtgtacac
     9301 ataaacatat gtatgtatac acataaacat gtatgtatac acatacatat atgtgtacac
     9361 atatacttat gtatgtatac acatacatat atgtgtacac atatacttat gtatgtatac
     9421 acatacatat atgtgtacac atatacttat gtatgtatac acatacatat atgtgtacac
     9481 atatacacat atacatatat gtgtacacat aaatatgtat gtatccatat atgtatatat
     9541 atacacatgt atacagatat gcatctatat gtatacctct atatgtatgc acatatacat
     9601 atatgtatac atatatacat atatgcatac atctataata tatgtatgtg tatatataca
     9661 catacagtgt ccacttaata tatatatcta tcttgtgtgt atgtgtgtgt aaatatacac
     9721 acacatacac acacgataaa atacagagtc taccacatga tgagcctctg ctaggtcctt
     9781 agcaatcaaa ccacatgtcc agtcctggcc cccattctac aactaaacac atgggccagt
     9841 ttaggggtcc aggagggcaa gaatggtggg tccacgtaga aaccaggtga gggaggagca
     9901 gtccacaggg ctggggtgat gggctggtga agcagtgttc caggagggga actgctgctc
     9961 acagggctgt cctggtcgcc ctcgggatac agccagactt gatccgagtg gctcccgggg
    10021 ctgaatgggg accgccgggt gcatatccca ggaggcagcc ttcagctcag tggggaaagc
    10081 agtttccaac cttagagctg cccaacacta gcacagggca cctgagaaag gaggggcccc
    10141 tctgccttta ctctgtctcc cactagaggc agctggttcc ttcagggaac tctggtgggg
    10201 ggtgaggggg tggctggttc tcagtgggca ggggtgaccc tactggggtc agtgggctgg
    10261 caatgctggt cttcactaag aagagttgaa aatagccagg aagctaagcc ctggctcctg
    10321 ggctcctggg cagatgctta attaggagga agaaggaacc aaaatcatga acacaactgg
    10381 ctctctcagg gggaggctgt caccctccaa gctcttcttc cccttcctca aatggagatt
    10441 cacactcatc cctagttcag gagagccgcc attgatgatg aggaaatccg tgtcaaagaa
    10501 gctggaaaga ctgctattca ttgtgagaat tttgtttcca ctgctacatt acattgtttc
    10561 ttcttgtttt cccttccaat ttccagttaa gaatctttca cagaaaattt ttaattttat
    10621 cacaaactgc acagatatca cacagctgca cccccatttg gtgacacaaa gcataccctt
    10681 ctgtgaagat tttcacttta cgccaaggca tgattgtcac tttacgccaa ggcaataaat
    10741 ttttacaaat tttgtataac aggagctgaa ttctgggttc tcaaatgtga aatgtggcaa
    10801 aaaaaaaaaa aaaaaaaaaa aagatttaat tcaagcattt tgtcatgtgg ttcttatttc
    10861 ttcaaccaag tttgtttaca gtcactgcct ttgaaataca gtcaaataca tctg
//
